2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

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Not much to say....happy growing

2nd time doing Brix levels...some ladies went from a Brix of 4 to a Brix of 10 while 2 only went from a Brix of 4 to 6. I am pleased with their progress upwards. Dam just learned you should take leaves for testing 2 hours after lights on....I took leaves from dark period 😣. Will update with new Brix numbers taken with lights on. Chopping is coming very soon. I will tag the Brix of each plant when I hang to dry so I'm able to keep track of how the higher Brix relates to taste, smell and potency😎 😁The ladies noticeably got fatter over night!(Nov 11th). Man they really enjoyed that last watering, hope this continues into next week. Just read to take your Brix leaves in a "Z" or "W" pattern as older leaves will contain higher carbs. The refracted light tells us the amount of carbs in the "juice or sap" and minerals too like calcium, proteins and fats (lipids). High Brix enables weed to produce secondary metabolites = greater medicinal value. Sucrose/sugar (Carbs) & mineral levels in the fruit or flower translates or relates to taste. A tasteless 3 Brix tomato from the grocery store or a 14 Brix tomato from OMG you grow delicious tomatoes. Death to covalent bonding! (My goal a 20 Brix😀 Won't be happening with this grow but will be the next😁). Read that most people can detect a half point difference in Brix in the flavour. Lots of smell coming from these Gorilla Girls😵 I can only go so far with plant chemistry and then I get a headache and get lost😔

Unfortunately, the plants have numerous leaves which look unhealthy (discolored, droopy, crispy/decayed). I removed the 3-4 worst looking leaves from each plant every other day, although I know that can be harmful to autos. Started adding in "Sensi Cal" this week to hopefully rebound from potential mineral deficiencies. Still using Recharge microbes once a week. Nutrients are now being administered accurately and at 1/4 the recommended level. Despite the unhealthy state of the plants, the pre-flowering bud sites look pretty good to me. I'm happy to see healthy looking pistils. Needless to say, I'm very eager to get these girls back on track and looking vibrant and verdant ASAP.

There is not much to say about week 10.. I just followed my feeding plan

Buenas noches familia, de nuevo actualizamos el diario, y acabamos la cuarta semana de floración, vaya brazos laterales que están tirando, se van a poner hermosas, se las ve sanas y vigorosas, nota mucho la forma sativa de sus hojas así que veremos lo que tardan en florecer. Ph controlado a 6,5 y humedad por debajo de 45% not bad las condiciones temperaturas mínimas de 20 y maximas de 27,5 Grados. Al atar las puntas los brazos tendieron a ir a la luz y gracias a eso la planta está tomando la forma de la esquina.

Giving these girls some good flushing out they only have 5-7day till harvest

D22/V20 - 14/09/23 - CO2 Dispenser is working good 💨💨💨 D23/V21 - 15/09/23 - Added some LST to bend her down 🌿🌿 D24/V22 - 16/09/23 - EC raised up to 1.0. 📒 pH trend is down, so I'm going to change the water in the next days 🧠 D25/V23 - 17/09/23 - Now CO2 is provided by Batman 😋 🦇🦇🦇 D26/V24 - 18/09/23 - Added LST and made some defolation 🌿🌿🌿. EC is 1.1 and pH is 6 📒 D27/V25 - 19/09/23 - LST and new bending 🌿🌿. EC 1.2 pH 6.2 📒 D28/V26 - 20/09/23 - All seems ok, but I noticed some strange spots on her. I'm going to investigate ️

A happy home and these girls are doing what nature intended! 🚀🚀🚀 ______________________________________________________________ The time has gotten away from me! I was over here using a dropper on these ladies when i realized the needed a full-blown drain to waste FEED!!!! I consulted the feed chart and stuck them on a week 2 regimen. I learned the 3gal pots hold 2 containers in veg and 3 in flower. good to know for measurements. There was a little yelllowing of the leaf on 2 plants but i think its from the neglect! Lets see what a delicious meal brings about!!! ______________________________________________________________ They aren't 100% happy, but I'm hoping they grow out of it! Not sure why one plant has all they yellow spots when 3 others don't They also arent drying out fast, i think it was a bit to early for a drain to waste. Struggling to decide when to water again

This plant has CRAZY stacking on it. It hasn't grown up as much as it has branched out. This is a strain I'm growing to treat symptoms of MS, so with the stacking it may turn into a mom plant for cloning, but we'll see. It's received some feedings of liquid seaweed, black strap molasses, and URB in R/O water.

Siamo arrivati al giorno del raccolto della Monster Zkittlez 🗿💙... Il bud principale è maturato un po prima del resto della pianta e nn so come mi son trovato semini, non ancora completamente sviluppati, qua e la per i vari bud. Il profumo ricorda l uva americana 🍇... Prendiamo le forbici e facciamo andare le mani☺️💪🏻✂️

They’re growing well. One of them is a bit behind.

papaya cookies has now completed her stretch. typical for papaya, she develops purple/pink pistils. such a beauty. hopefully the weather will stay good enough for her bulk.

I started the week by selecting the 3 best plants to put them into their final 26l pots with some Dynomyco. I made the selection based on their sizes, shapes and roots. I increased the light output from 25% to 50% and started the week with 23h of light per day. After a few days I noticed what I think are light burns on every plants so I reduced the number of hours/day to 18h and it seems ok now. I checked the pH, not optimum but alright for soil and I always have this 7.0 with this soil and this pH meter (don't know if it works properly or not because it's always 7.0 in this soil, never 7.1 or 6.9). The 3 plants grew a lot this week, it's one of my best growth rate so far so I'm very happy 😁 The Wedding Cheesecake is by far above the two others, it already developped 5 nodes so I topped it (first time it happens to me that soon). As usual with this soil, I think they have a bit of nitrogen toxicity but nothing critical. Plants heights at the end of the week : ------------------------------------------- Gorilla Cookies : 18.5cm Purple Lemonade : 15cm Wedding Cheesecake : 22cm

I did the last and final defoliation; I also I tutored. Looking forward to flowering.😗

Hello everyone, I would like to thank you all for your likes and comments, it really motivates me to continue. This time it will be a special diary for me because I received from FastBuds all the girls who participated in it as a prize in the competition. @Fast_Buds The seeds began their adventure on cotton pads, and I used distilled water as the medium. After only 20 hours, cracks appeared, and after less than 3 days, the sprouts were over 3.5 cm long and ready to be put into pots.

25/01 Still having problems with the heat, I've build a exaust with 2 120mm computer fan, this week has arrived the humidity controller and I noticed that is to low for the temperature. Now I waiting for a mist maker to build a DIY humidifier(using a simple water spray for now) I belive this will be the solution to get a better VPD and control this fucking heat inside the tent 😎 30/01 The temperature is reaching more suitable levels, between 25-27°C. Even with the rainy days and higher humidity outside, the grow is stabilizing well at around 65% humidity.