2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Día 6 (29/04) Spray solo agua el top del sustrato. DLI 13 Día 7 (30/04) Spray solo agua el top del sustrato. DLI 13 Día 8 (01/05) Spray solo agua el top del sustrato. Subo a DLI 17 Día 9 (02/05) Elimino la cúpula casera de humedad y empiezo a regar con 300 ml solo agua Día 10 (03/05) Empiezo a luchar con una ola de calor inesperada para esta época, que me trae temperaturas exteriores de 30 ºC y humedad relativa del 30%... Mantengo la cúpula casera de humedad Día 11 (04/05) Hace muchísimo calor.. 30 °C. A ver como lo llevan Hago un invento casero para aumentar la humedad Día 12 (05/05) Primera alimentación con Aptus! 300 ml H2O + Regulator 0,15 ml/l + CaMg-Boost 0,25 ml/l + Startbooster 0,25 ml/l - pH 6.2 Creo que las plantas se ven un poco pequeñas para tener 12 días... esperando por explosión de crecimiento en los próximos días.... 💦Nutrients by Aptus Holland - www.aptus-holland.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

Smooth week. This is a beast of a strain, wile o have two on the pot, since i germinated two seeds, i have planted both, but the two are beautiful i cannot kill one and choose… i have always wanted to plant this strain, I’m very happy i did! It is growing beautifully. I’m using velokelp from Remo and Nature’s candy i had laying around. That is compatible with living soil, and Moringa extract once a week. And have planted some companion plants as a soil cover. The Fastberry is in a super soil, next time all will be in a super soil… So easy, top up with some good bacterial / fungae , humic fulvic, seaweed and it is all good. Or straight water! Very minimal.

Hello my friends 👩‍🌾👨‍🌾, This 8th flowering week was good 👊, maturation Buds continue 🌲❄️, I thought it will be the last week, but need one more. The end is for this week end I think, for moment trichromes not really full milky, and I like something like 20% amber. I've started flush with flash cleaner The senesence is started on the #3. 💦 1 Watering this week 1.8l/plant . Water + flash cleaner PH@6 Lamp @100% Bisous 💋😘, and see you next week. Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Mars Hydro - TS 1000 https://www.mars-hydro.com/ts-1000-led-grow-light Mars Hydro - FC3000 https://www.mars-hydro.com/fc-3000-samsung-lm301b-led-grow-light Mars Hydro - SP3000 https://www.mars-hydro.com/sp-3000-samsung-lm301b-greenhouse-led-grow-light The High Chameleon - Bisous Au THC 💋💋🌲🌲😘😘 https://www.thehighchameleon.com/shop/bisous-au-thc-83

MrJones Pink Sugar Breath F2 by VMAN - ViparSpectra XS2000 240W Infrared Full Spectrum LED Grow Light ´🌿⊱╮🌿╰⊰🌿 WEEKLY GOALS 🌿╰⊰´🌿╰⊰´🌿 🏡Indoor - 3"x4"x8" Custom Built Grow Closet 🌞Environment - 80F and 65% Humidity - using Humidfyer as needed. 🌾This strain likes to stretch during flower, so a heavy training regiment will be needed. 🗓️Vegging ⚱️1-Gallon 📊6.4 / 6.8 PH 💧 Feeding / Dry Amendments using Dr. Erth Live all Purpose and Using Up my Mr. B's Green Trees ORGANIC BLOOM 2-8-6 🌞ViparSpectra XS2000 240W x2 Infrared Full Spectrum LED Grow Light 🕷️ IPM - We will be using Green Cleaner" 1 OZ per Gallon, and CannControl from Mammoth alternating between products each month for Integrated Pest Management. 🌿╰⊰´🌿╰⊰´🌿 PLANT UPDATES 🌿╰⊰´🌿╰⊰´🌿 📝 Notes - A good friend of mine gave me some of his crosses to test, please read below for his amazing details, I am very excited to see what I can accomplish with these genetics and my cannabis gardening approach. ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 🗓️ Monday 09/12/22 Plants are doing very well, removed a few more fan leaves to continue to expose lower growth, getting very close to topping, this will allow the lower growth to catch up and start to bush out. Just using Dechlorinated H20 PH to 6.4 every few days. 🗓️ Tuesday 09/13/22 Went ahead and topped these ladies, now it's time for the side branching to grow up and the plants to bush out. 🗓️ Saturday 09/17/22 - The last day of week 4 has arrived, these plants are doing very well, with a very tight internode spacing, I am trying to embrace that, as these buds can stock up and exceed 12-inch heavy colas, I am in no hurry to flip these plants, I want them to grow out and fill the closet vertically, tomorrow I start my first round of IPM, I gro in soil organically, so will need to stay ahead of pests. Check out the amazing pictures from today after mild defoliation, I placed a few plant trainers on 1 plant just to see how it would respond I will be using CanCotrol.by Mamoth. ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 Breeders Notes by VMAN Pink Sugar Breath is a creation of Platinum Kush Breath x Pink Sugar cookies originally Bred by UFO Genetics. Pink Sugar Breath F2 is the second filial Generation that was hunted and creates by Visions. The vigorous cultivar takes 63 to 75 days to flower with various phenotypes. These phenotypes can express terpene profiles ranging from Bakery Dough, Sugar, Og Funk, Fruity, Gas, Dank, Chemical Floor Cleaners, and much more. Expect Purple and Pink Phenotypes to pop up fairly easily. Color starts to show within 7 to 8 days of flower for the purple and pink phenotypes. Green phenotypes have more of an OG Funk/Floor Cleaner presence ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿 ╰⊰´🌿

Look out for my next grown fast budz gorilla cookies and a other crack at blueberryz

Die Pflanzen haben deutlich mehr Durst. Eine halbe Stunde nach ihrem Sonnenaufgang sind sie zweimal in Folge sehr trocken gewesen. Die Spitzen der Stempel an einigen Blüten haben sich leicht braun gefärbt. Daher die Wassergabe jetzt auf 3,5l Täglich erhöht. 2,5l eine halbe Stunde nach "Sonnenaufgang" mit normaler Düngung und ca 5h später nochmals 1l jedoch mit halber Dünger Dosis.

Very easy grow so far hardest part is trying to control room tempreture....plants really like the mid 70s for tempreture and a bit cooler at night...plants are growing so fast you can notice every time I look at them

So, eventful week. Prior to putting my seeds into my system, my light and rdwc system was left on as I was dialling in the growing environment before placing the seeds as a final home. My only issue was I had a light leak BEFORE putting the foil top covers on the clay pebbles. Long story short, I got algae which ended up turning to root rot. I spotted it very swiftly and did a res change adding beneficial bacteria to the system along with the base products of remo nutrients. A few days later new growth and recovery is taking place. Day 16- Fully back on track, new roots shooting out into the water and the growth rate has picked up as it should. Foliar feeding twice a day for a little boost until the root system establishes a bit more. Huge changes in just a day. Can't wait to see what a week's growth looks like. Really loving the sunset/sunrise feature on the trolmaster, something I got to experience watching recently! Watching by closely to make sure everything stays in track. Stay tuned for the next one 😉

[Tag 6]💧💡 Zum ersten Mal mit Dünger (pH 6,0 / EC 0,8mS/cm) gegossen, ca. 5,5 Liter. Eventuell zu hoher EC- Wert, vielleicht ist ein EC- Wert von 0,4mS/cm am Anfang optimaler. Beleuchtung 25% (80 Watt), Abstand 65cm, 18 Stunden (08:00-02:00 MESZ). [Tag 7]💧 Hinten links etwas nachgegossen, ein paar hundert Milliliter. [Tag 8] - [Tag 9]💧 Auf der rechten Seite wurde das restliche Gießwassers nachgegossen, ca. 0,5 Liter. [Tag 10]💡 Beleuchtung 25% (80 Watt), Abstand 50cm. [Tag 11]💧💡 Mit Leitungswasser (pH 6,0 / EC 0,4mS/cm) gegossen, ca. 10 Liter. Beleuchtung 50% (150 Watt), Abstand 100cm. [Tag 12] Alle Sämlinge entwickeln sich sehr gut.

day 15 of blooming... streching is on full force and getting bigger by the day.... tent is filling up nicely. at the end of the week 3 will do a defoliation for a better airflow and light exposure.

On Dez 25 I flew to Thailand so my father had to gave the planst nutriens and water I Wrote him a what and how much he should give .

These gals are growing fast however I think I should have fed them more calmag this week. Their feeding week 6 will be heavy calmag and I will be bringing up the bloom nutes.

6/19 Knowing it was going to be over 100° for the majority of the day I WATERED the garden with 3 1/2 gallons. I went to check around 4 and everything looked amazing. We got some hard rain from a thunderstorm at 4pm but it seems like these girls like it rough and did fine. The take a beating and come back stronger. Knock on wood but I haven't seen any problems on the plant I snapped amd dict taped.. Everything seems to have toughened up and the leaves are an awesome healthy color. They grow observable every day now. Tomorrow is supposed to reach 100 again for most of the day before weather goes back to semi normal. I took a video but it will take way to long to upload. Tons of other pics didn't upload either. 6/20 Another day that's going to be over 100°. Plants seem to love it lol. I can't believe what I'm seeing. Bags still had some heft so I didn't water this morning. I figure I'll check them around noon and then plan on watering tonight if they need it. Hopfully they'll get some rain from thunderstorms. This crop has been through a lot. I think that has helped them significant deal with this unpredictable weather. 6/21 We had a MAJOR thunderstorm with sheet rain yesterday. I missed it but it was intense. I saw some videos. First time dad seemed concerned. Fortunately there was no damage. I was going to water today but I decided to hold off due to the look of the plants and the "praying leaves" at 4pm and feeling some moisture in the soil. Bags seemed to still have some heft. I'll probably water tomorrow. Need to transplant the 1 gals and need to figure out what I want to do for nutes this year. So far I've put in much less effort and I'm still doing great. I just don't have as many plants as usual. 6/22 Rained last night. Not much but the bags were heavy still so I held off watering. Plants looked happy and I can fix under watering much easier. It will rain tomorrow. I'll check throughout the day and update but things are going good. I should transplant those 3 seedlings in 1 gals. 6/23 It Rained last night and is suppised to rain today. It was raining when i went over. Just the kind if rain the plants love. I'll check again before church but I'm eventually going to have to water them. Those thunder storms helped a lot. 6/24 Rained about an inch yesterday. Plants loved it. Bigger everytime I see them. One seedling showed a pistol and I'll transplant it with the rest. It was the only one I was worried about. I've seen a FEW leaf hopper punctures and some other bites that could be anything. Time to get the ipm 6/25 Looks like its gonna be a nice sunny day. 75° at 10am. Plants are doing amazing and growing at a rapid pace. Haven't done anything ipm as of yet but any damage I've seen is localized to "A" leaf on "A" plant. I'll be paying attention and watching for signs of deficiencies. As of now though they LOOK great. I'll try to transplant the 3 seedlings today.

- DAY 57 (30/07) First of all I would like to thank NobodysBuds for the time they have given me, giving me tools that I was not aware of. This week I’ll be back to feeding NPK. So, yesterday I started applying 4-way style LST. Everything that I will do relevant in the days of this week I will update it here in Week Comment. I am open to advice, I would be happy if you comment with what you would do in my place. - DAY 58 (31/07) FEEDING I reintegrated the power supply by decreasing K in favor of N I irrigated 2.5 liter of water with: - 2ml Micro - 1.25ml Bloom Solution PH: range 6.6/6.9 - DAY 60 (02/08) DEFOLIATION Cut some leaves at the bottom Day/night air temperature on average for this week: Day 31-36 °C. Night 22-26 °C.

Week 16 (Harvest) 1-2 Drying day 1 Temperature: 24.5 to 19.5 degrees Humidity: 65% to 50% The girls are ready to be harvested! Before chopping them down, i took a lot of pictures. I was able to remove most of the soil from the Biscotti #2 to see the rootball. I hang the plants as a whole to have a slow dry. The exhaust fan is on setting 4. 2-2 Drying day 2 Temperature: 19.1 to 17.5 degrees Humidity: 62% to 57% I changed the exhaust fan to setting 2 because setting 4 was a bit high in my opinion. 3-2 Drying day 3 Temperature: 19.9 to 18 degrees Humidity: 62% to 59% Today i checked up on the drying plants, and i see there are some buds touching other buds, so i chopped the plants in half. The buds are still to the main stem for a slow dry, but are hanging more spreaded out to prevent any budrot issues. I changed the exhaust fan to setting 1, as someone told me that it was enough air movement for drying. 4-2 Drying day 4 Temperature: 19.8 to 18.5 degrees Humidity: 62% to 59% 5-2 Drying day 5 Temperature: 19.9 to 18.8 degrees Humidity: 62% to 59% Today i checked on the drying plants and the buds are shrinking a bit, they are getting a little bit crispy on the outside aswell, I hope they wont dry as fast, and i aim for a 12/14 day dry. For the next grow i already germinated 2x Chemdog (GreenHouseSeeds) 2x Gorilla Kush (GanjaFarmerSeeds) and 1x Deep Candy CBD (GreenHouseSeeds) And I have some upgrades planned aswell! I bought a autopot kit with 4 15L pots, this way the plants will have water and air 24/7 whenever they need it. I have seen alot of great results using autopots so i am very exited to try it out! And I bought a small camera so i can shoot some weekly timelapses! Feel free to Follow me if you like to get notified for the upcoming diaries! Thanks for checking out this diary, peace! (More harvest updates coming in the next couple of days!) Huge thank you to Zamnesia for the seeds, and Plagron for the collection of nutrients, and the opportunity to participate in the POWERBUDS contest! It was a fun and learningful adventure!

Welcome to week 12 and finally the last week of my grow diary. As predicted, the weather has taken a turn for the worse, so I've decided to harvest. If the weather had been better, I would have given it another 1.5 to 2 weeks, but unfortunately, there's nothing we can do about that. The outdoor season has therefore come to an early end. It would be great to live in a place where it works all year round without any problems. A brief summary of Sour Jealousy: This strain is simply fantastic. Super easy to grow. Apart from a minor incident with mildew, I had no problems. The citrusy, creamy terpenes are right up my alley, and I hope the effect is too. In the end, the plant grew bigger than I had imagined, but I was able to see from other diaries how the plant would develop. Chubbs Sour Jealousy was even bigger. This strain will definitely end up in my indoor tent again so I can get the max potential out of it. See you next week for the conclusion of the diary!