Lst training and lolipoped

The addition of an enzyme has produced amazing results that were shocking and worrying at first. Massive rise in EC and PH every 24 hours from 5.3 - 6.8. Research yet no sign of any harm to the plants, in fact they seem very happy! The enzymes has created an environment friendly to the beneficial microbes and fungi that has lead to a feeding frenzy (Which wouldn't have happened ad I used Hygrozyme from the start.). EC rises a point or two as the microbes and plants expel waste and create a plethora of negative charged hydrogen ions. Thus, the PH rises. Never above 6.8, strangely. Because the microbes are eating and shitting so well, they have added a lot of chilated nutrients that are easier for the plants to uptake at a wider range of PH (Yes, some as high as 6.8). The fact that there are so many bunches of calyxes sprouting and exploding, filling in the flower space is proof enough for me. Blueberry Headband (2) from Humboldt Seeds. https://www.humboldtseeds.net/en/blueberry-headband/ Lighting https://www.horticulturelightinggroup.ca/products/260w-qb-v2-led-kit DWC Nutients https://generalhydroponics.com/floraseries FloraGro .5 ml/l FloraBloom 2.5 ml/l FloraMicro 1.5 ml/l Cal Mag 1.5 ml/l https://generalhydroponics.com/calimagic  Diamond Necter: N/A https://generalhydroponics.com/diamond-nectar SuperThrive: .5 ml/l https://superthrive.com/ Epsom salts Tarantula https://www.advancednutrients.com/products/tarantula/ https://hylineproducts.com/products/hygrozyme/#one Grozyme: 2.5 ml/ltr Brown Slime Root Rot Organic Microbe Tea 4 liters RO or tap water 5 ml (1 tsp) organic sulphur free molasses 5 ml Alaskan Organic grow fertilizer (or something comparable) 5 ml Alaskan bloom fertilizer (or something comparable) 5 ml Seaweed or kelp fertilizer 2-4 ml Advanced Nutrients Tarantula

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 36 since time change to 12/12 h. Hey guys :-) . The buds developed really well this week 👍. They get wider, bigger, and firmer. They are already starting to smell very tasty. This week it was poured 3 times with 1.2 l each (nutrients see table above). Unfortunately I found a couple of single trips that tell me that the trips still haven't completely disappeared. Since I'm not a neem at this stage I will inject more oil and the lady has already finished over half of her cycle, I will let her run like this and the newcomers will be treated again 👍. So I hope that at the next round all of them will finally be gone. I had the problem more than 5 years ago and it took a long time until everything was ok again and there was no animal left. Otherwise everything was checked and everything was cleaned. I wish you a lot of fun with the update. Stay healthy 🙏🏻 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. You can buy this Strain at : https://www.amsterdamgenetics.com/product/kosher-tangie-kush/ Type: Kosher Tangie Kush ☝️🏼 Genetics: Kosher Kush X Tangie 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + Nutrients : Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4

Every plant in garden day 56 Noticed yellowing mid week 7 don’t think it fade having some ph issues my run off is 7.2 after flush then watered again still up at 7 so hopefully going to water at like 6 6.1 from now till it goes down growth continues tho some buds getting big ☺️

Week 23 - 29 July 25 July - feed day 27 July - feed day (1.3 L)

Welcome back boys, A little update with those rock hard colas, the finish line is being seen not far away on the horizon. I hope they gonna fat a little bit, I think we all wish the big fattening deep down ... Stay tuned, stay stoned

I started the GSC seeds from HSC by soaking in a glass of water overnight then planted in coco coir under a humidity dome in my veg tent. I will plant them in flower box in a couple weeks when they can handle the feed that my BB clone is on right now. On day 5 my 2 other Gsc seeds rotted perhaps too wet but I have one good remaining for this run. I started White widow seeds and will run 2 phenos. Gsc is looking good so far.

With my 30x jeweler scope i barely see clear and it’s my first time inspecting trichomes so i hope she finishes at goal of 90%milky as in the eyes they look more clear but more attention to it i think it’s ready according to colors and fastbuds guide chart will intentionally harvest on week11 day1 (from seed) hope it’s not too early

week (16) 3 weeks into the flowering stage. Slight increase in the nutrients. This past week we had some hot muggy weather along with some nice rainfall. The girls loved soaking it up!

🌿 Woche 3 Eine weiter recht normale Woche. 🌱 Wachstum Beide Damen haben einen schönen Wuchs und Grünton. Die anfängliche leichte Verbrennung hat sich perfekt rausgewachsen. Nun sollte die Livingsoil den Job super ausführen können. Noch sehen sie beide auch sehr identisch aus. 💧 Only Water Ich habe mich dazu entschieden, meiner Livingsoil ein wenig mehr unter die Arme zu greifen. Ein Grow-Kollege hat mir verschiedene Dinge empfohlen, die man machen kann. Ich habe mich dann für 2 Mittel entschieden, die mein Only Water Experiment nicht aushebeln: 🔹 Effektive Mikroorganismen von Multikraft 🔹 Mycorrizae von R&R Beide Mittel gelten nicht als Dünger für die Pflanze, sondern als Booster für den Boden. Dementsprechend kann man diesen Run immer noch als Only Water bezeichnen 💦✅ 📟 Blumat und Gießen Ich freunde mich immer mehr mit dem Blumat Digital an. Ich wüsste nicht, wie ich ohne ihn durch den Mulch den Gießzeitpunkt ermitteln sollte. Rumwühlen oder den Topf heben ist leider keine Option ❌. Deswegen bin ich wirklich froh, dass es ihn gibt 🙏. Ich gieße weiter nach Fahrplan: 🕒 Alle paar Tage bei 110 mbar 🚿 2 l Wasser Diesmal habe ich etwas EM Bodenbuster mitgemischt. Damit hab ich dann den Boden mit zusätzlichen Mycorrizae geimpft. ☀️ Wetter Wenn man bedenkt, dass es tage­lang locker über 33 °C heiß war 🌡️🔥 und ich dazu keinerlei RLF-Unterstützung hatte 💨❌ – sehen beide Damen top aus ✅. Manchmal denke ich: „Oh Gott, geht das gut?“ 😅 Aber ich lass es drauf ankommen. Und bis jetzt läuft alles nach Plan 🛸✨ 🎶 The Grow must go on! 🌱🔥

finally the scorching sun came having moved the plants from under the canopy outdoors a downpour created a bit of mold in a small part of the skywalker and also to the little cherry i later removed it and sprayed the plants with garlic infused water now i'm the bomb i'm fertilizing with long awaited organic vermicompost, the plants are perfect, i think i will delay the harvest for the cherry to make it gain a little more weight at the expense of the flavors the flavors are amazing absurd...i ordered some clones by freakshow humbolt seeds I also made new transfers with old seeds tried to germinate and they went splendidly.honey cream and special queen by royal queen seeds

This plant only started flowering after I switched it to 12-12 Not an autoflower This plant also has a mystery graft! I grafted a few and only one survived but as I did not label them I’m not sure what it is Could be RQS royal Cush or northern lights automatic both indica plants suppose to be autos. Bare in mind this royal jack is sativa! It seams to be ok I will be providing it more light to incourage it to flower Looking forward to seeing what the flower will be like.

4 really health plants, this week time to place them in bigger pots. Update 7-1-23, transplanting the plants from the cups to a bigger cup. I was a few days late because the roots where forming in circles in the cups. Because the stem was not very stiff, i added a little bit ventilation in the tent. See you next week.

Hi all👨‍🌾👋 Welcome to my another week update Hope everyone keeping well and having a great week 🧑‍🌾🤤 Week 2 Feb 9 - Feb 15 Very easy and steady week. Kitana and Aphrodite doing absolutely fantastic. Watered 2 times this week with approx. 300 ml each and on Feb 12 introduced foliar feeding, at start with few drops of biobizz fish mix in 100ml solution. They responded flawlessly😁🤤🧑‍🌾 Have a great and successful week everyone ✨🍀 Much appreciate all your likes, follows and comments. 🙏💚❤️💜 Peace and love brothers and sisters 👨‍🌾✌️💚 Links https://2fast4buds.com/seeds/TROPICANA-COOKIES-AUTO https://www.biobizz.com/ https://fishheadfarms.com/

Vegetative Steering 71f/60% 6:30 220 P1 6:49 440 P1 7:08 660 P1 7:27 880 P1 7:46 1100 P1 8:09 1320 P2 10:45 1540 P2 11:04 1760 P2 03:00 1320 P3 Day 14 Plant training shows most here. I do not like the look of a doubled topped plant.(will continue to do one top per 6 week Veg) Day 15 Secret sauce microbes 1ml per. Day 16 Tricantonol Spray(150ppm) Did that spray fix the sick plants? I think so 15 of 16 plants have preflowers. the 7 week veg plants are my favorite. 7 weeks is good if i will be topping twice The third fan was crucial to humidity control Day 17 Sprayed 6 plants(pictured right) with Jas wondering if these 6 will have stunted growth. Either they will have a stress response and halt root growth to early OR they grow normal but are a but frostier These 6 will be sprayed a second time during week 4 BEFORE the bud sites are medium sized. The other 6 will be sprayed once wk4 qnd once wk 5

Cherry Cola diesel smell ⛽️

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.