Taken all 3 down today 01/09/22 looking very good 👍 looking forward to trying it

Update 11/04/2022 (Day 57) Hello All! the start of yet another week!! it's been 2 weeks ago since I Flipped to flower but I have a feeling they are really slow in this process. it's only today I really start to see some pistols forming! Since these are my very first Photoperiods i am yet again on unknown territories. Might be that at this stage my TSW2000 just isn't juicy enough. lucky enough I've got a new FC-4800 coming in from Mars hydro. this will have a much better coverage and and some more wattage I might have to do another Lollipopping session once I really see pistols all over the place. The stretch on these girls is crazy at almost an 78CM as of today. I only have +- 80 CM left in my tent and that also means I gotta figure out a way to get my lights up tight against the ceiling and rearrange my extraction fan. 1 plant is kinda on the slow side I accidently topped that one in the early stages and it feels like it never really recovered from that. Having a look at my clones well 4 out of 8 survived and are now starting to develop some nice roots. Thats it for today. hope too see some nice buds forming this week. Update 14/04/2022 (Day 60) Yesterday the new light arrived so I got busy. in order for the new light to be hang as high as possible I had to move the extraction fan outside the tent it now sits on top of the tent. the driver from the light is also placed on top of the tent. I must say this new FC4800 is a very welcoming upgrade it really has a much better coverage in my 4x4 and more wattage :D after the switch I got busy with some scissors to apply a final lollipop session. also transplanted the clones in some bigger pots gotta keep them under the Secret Jardin Tneon 2x55watt lights 6500K till beginning of may than they will be moved to some friends garden.

Well, what we feared, became a reality, one of the regular XTrakush plants is a male. This is why I planted two and I'm happy it seems to be only the one. But with the canopy going nice and full it hurt to get it out. Of course, I hoped to have two females but nature went its course. Considered leaving it in, to pollinate the others, or keeping it somewhere, but I do not have the space or light for it. Thus with pain in my gut, I went about getting her out. I notice a nice developed root system, very healthy, which was very hard to get loose. After shaking the soil off I have a rootball the same size as after my whole grow last time. I could lift the whole 57 ltr bag by lifting the plant. Sturdy stem, thick solid branches. This is what I was aiming for and it works. The soil again is proving itself to be a winner. The light this low gives some higher temps as expected, but manageable within ranges. The SP-3000 is performing really well and I cant wait to see the bud development coming weeks. I'm sure it will deliver a great amount of bigger buds looking at all the flower sites and the thick branches that support them. Remember to check in on www.mars-hydro.com, they have awesome deals now for Black Friday! I'm giving more water now, once in two days instead of three. They seem to need it with the higher temps. The plants seem to take the light well on only 45 cm. I've started adding the BAC Organic Bloom Nutrition. It is designed to work with the microorganisms I added with the micro life. With this I do a little bit of Calmag, but not so much, since I don't see any evidence of shortages, again proving my soil is dialed in for growing under LED perfectly. All in all, even with a minor setback, all is ready to pull in a mega harvest. Thanks for checking in again!! Hug Bud & Sunshine.

Last week I think. All cloudy trichomes and more ambers everytime I check. Leaves are yellowing themselves they've faded a lot in the last few days. The main stem split from 2 top branches so I taped it up. Dropped the terra bloom and gave just dragon force and sumo boost last feed. Going to start flushing this week and see if I can go a week at least. May have left it a little late. Smell of blackcurrant is amazing, got a really strong almost sickly smell to it aswell though, not the most pleasant hahaa mext update will be harvest thanks for sticking through on this one ✌️🏻

So this is how things are looking for the ladies at the end of Week 5 of Flower, I have uploaded a video for you guys with all the information, any questions just ask away 👍🏾👊🏾😎

Good seeing the difference in each done some LST with them all but the middle 2 in the 25L I’ve did both of them different to see the outcome Fiming with one topping on the other and some defoliation on one and not the other Newbie goals practice make perfect 😂✌️🏻

So were on day 26 of flower there looking good getting a nice smell starting to throw out trichomes Have down loaded a thew videos of before and after removing fan leaves

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Anche lei è l'ultima settimana di lavaggio radici.......oggi 29/09/2024 taglio questa Ayahuasca purple...ha un profumo fortissimo.... Oggi probabilmente inizierò a pulire tt le cime e poi.via un taglio netto 😂🤣😜👍💪

Bienvenidos de nuevo, esta semana empiezo a reducir la EC, por razón de no quitarle todo el alimento de golpe, probablemente empezaré con el flush en breve, debería quedar 1 semana para la cosecha. Como trabajo con un sustrato inerte(coco), pienso que no es necesario un lavado de raíces, y creo que a partir del flush no necesito dos semanas de riegos con agua porque creo que con una es más que suficiente para que quede limpio, así las niñas comen una semana más de lo previsto. Iré actualizando durante la semana. Un saludo!

This is a disappointment. I got 1 out of 10!!!

8/24 Week 6 Flower Bea is the big girl of the tent, not by a lot but significant. Hero is not far behind and looking great. Reducing Grow nuets will Increase Bloom as we go. Want a lower EC with lots of runoff for tonight, been pretty high and its going up from here. Looking good what to say? Stretch has started but its very reasonable 8/25 They added an inch and some over night Nuets changed to all Bloom 8ml/gal A&B and Koolbloom 1ml/gal EC at 1500 see if we can keep this until ripening 8/26 Resuming twice a day feeds 8/27 Giving a dose of Kangaroots for their early flower root building this is the last of that. Defoliated lowers removing all under growth Tent is getting pretty full... maybe they are like goldfish... 8/28 Growing @ 1/2 - 1 inch per day still

10 semanas desde germinación día 11 de abril hasta corte viernes 25 de junio (día 69 corte) cultivada en maceta de 11 litros con tierra plagron all mix, inicio de floración en semana 5, día 20 de mayo

Plants are really flowering still small but plant one has a lot of tops now and plant 2’s buds are already getting dense.

Legend Timestamp: 📅 EC - pH: ⚗️ Temp - Hum: 🌡️ Water: 🌊 Food: 🍗 pH Correction: 💧 Actions: 💼 Thoughts: 🧠 Events: 🚀 Media: 🎬 D: DAY, G: GERMINATION, V: VEGETATIVE, B: BLOOMING, R: RIPENING, D: DRYING, C: CURING ______________ 📅 D29/V25 - 14/05/24 ⚗️ EC: 1.0 pH: 5.2 🌡️ T: 20°C H: 50% 🌊 🍗 💧 💼 🧠 🚀 Back Home 🎬 ______________ 📅 D30/V26 - 15/05/24 ⚗️ EC: 1.0 pH: 5.0 🌡️ T: 20°C H: 50% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D31/V27 - 16/05/24 ⚗️ EC: 1.2 pH: 5.0 🌡️ T: 20°C H: 60% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video very short + 1 TL video of LST ______________ 📅 D32/V28 - 17/05/24 ⚗️ EC: 1.2 pH: 5.0 🌡️ T: 20°C H: 60% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D33/V29 - 18/05/24 ⚗️ EC: 1.2 pH: 5.0 🌡️ T: 20°C H: 60% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D34/V30 - 19/05/24 ⚗️ EC: 1.0 pH: 5.0 🌡️ T: 20°C H: 60% 🌊 🍗 💧 💼 Big work to prepare her new home 🧠 🚀 🎬 ______________ 📅 D35/V31 - 20/05/24 ⚗️ EC: 1.0 pH: 6.0 🌡️ T: 20°C H: 70% 🌊 16 L 🍗 CalMag - Grow A-B, B-52, Bud Candy, Rhino Skin, Hydroguard 💧 💼 🧠 🚀 The new home for Rey in now ready 🎬

Ende Blütewoche 8. Die zwei Seitentriebe sind mir ganzschön abgehauen, richtige Brecher. Sie hat auch einen sehr süßen leichten Duft bisher, geht etwas neben der Pineapple Express unter, bin deshalb sehr auf das Glas später gespannt. Overall aber eine richtig tolle Pflanze mit den fettesten Buds im Zelt :D

unfortunately it was a very rainy week. i hope that no bud rot will develop. due to all the water one branch broke under the weight. but i tied her tightly and she is still doing well. she smells incredible and it is getting more and more intense. let's hope for a few weeks of sunshine until harvest and that everything goes well. power buds from plagron is definitely good for her.

Day 8 Finally got to check out the plant. Still on same nutrient plan since I'm concerned still about the white dots that are visible on the video I posted. Still very happy to see the growth on the plant. Plant is now receiving about a third gallon of water every other day-ish with 15 ml/gallon of botanicare kind grow nutrients. Day 9 Saw evidence of spider mites. Don't know how they got in since I'm in a grow tent. Got a treatment the guy at the hydro store said would work best since I'm in a small er tent. Sprayed leaves heavily with the spider mites killing solution and turned the lights off since the remedy calls for it and fed 8fl oz water with ~1 ml botanicare. Also bought Canna Coco A+B since I was at the store anyway. Day 10 2-28-18 Evidence of some type of nutrient burn can be seen on some leaves. Possibly a reaction to the anti-spider mite treatment. Any info is welcomed. Opting to keep light on instead of treating for spider mites since I think the spider mites problem is less of a problem than the new discoloration on the leaves Day 12 3-2-18 HELP!!! Anyone please look at the picture I posted and help me with what I need to do asap. TGIF because maybe I can spend the weekend saving this plant. Day 13 3-3-18 Removed the cup from around the plant and added some soil. I think it looks healthier than it has and think it's going to make it.

Shes looking beautiful 🌸💕