First week of flower in the bigger tent and on the Autopot xl smart pot system. Trying a new product on this run which is rock resinator which starts on Monday. Will leave these now to grow through out each week till day 20.

This fifth week started off kinda rough. I had to remove some sick leaves that got hit by leaf miners. I’ve cleaned up all the damaged parts from the plants, including this one. On Monday, I’m gonna spray them with a ready-to-use mix that has Neem oil, nettle extract, and horsetail. I also noticed a slight nutrient deficiency, probably because until now I was only watering with untreated tap water — didn’t check the pH at all. Even when I fertilized, I still used water with no pH adjustment. I’ve only just started using pH-controlled water like two or three times now, since I finally bought a pH meter. Now I’m watering and fertilizing with everything adjusted to 6.5, so that’s something I need to keep in mind from now on. I’m planning to bump up the fertilizer to 1 ml per 500 ml of water. I’m using Biobizz Bloom, and I wanna keep the dose like that because the pot is small — just 3.2 liters — so I don’t wanna overdo it with 1 liter of water. I’ve noticed that 500 ml works just fine, so I’ll stick to that. Other than that, everything seems to be going okay.

Not much to say about this lady. She has a lovely structure and has a great canopy for only being topped twice. Her buds look fatter and wider than the others. Can't wait to see her develop.

Defoliated and in its flowering pot, switched to 12/12 on day 35 she's stretching out nice. Will start feeding from week 7.

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July 7th makes week 11 for these ladies. They were supposed to have been harvested this week but we're a ways away from that. We have a bit of maturation and swelling to do yet. Maybe I stalled them, could be genetics, could be a combination of both? We'll see. I'm just happy they look as good as they do and to have had the helpful growmies I've had here giving me tips and help along the way. Thank you!

Normally I allow two days in the paper towel germination. This one after only 24 hours.

ladies are doing fabulous!! growing incredibly fast. loving the cob light. ww/bb you can literally watch grow every day. the white widow didn't like that I took a few fan leaves I took off, seemed to make her grow a tad slower. the ww/bb with the 3 axillary buds grew out perfect and the bottom node grew just right to get even, and make a nice flat canopy. same with the king tut. on crazy anomaly was one branch on the ww/bb has 5 auxiliary buds! never seen or heard that before. trying to decide If i should top there too. check out the video to see it better thanks for following any tips please lmk!!

Finally got the big tent and got the environment dialled in. With the AC in the tent, it's way more stable now; this also means I can close the distance between the light and the plant and she's definitely growing better.

Day 57 - Starting week 9 off with a gallon and a half of water and nutes today. She’s doing really well and getting big. Finally really starting to bud up a bit and not just pistils here and there. Not really doing any defoliating until she’s buds up a bit more but she’s getting there. She’s still stretching like crazy and when she seems to slow down, she gets watered and starts putting on the inches again. Day 58 - Didn’t really do anything with her today. Just took her out of the tent to give her a look to see how she’s doing. It looks like she’s definitely starting to bud up and should be an amazing harvest, when the time comes. Day 59 - Looking good and starting to stack up nicely. Still has got a long was to go but all is looking well. I did chop off a couple of lower branches that are completely covered and I’d rather her focus her energy on the ones that are getting the light. She’s gonna need some water again by Saturday. Day 61 - Gave the girl about a gallon and a half of water and nutes today and removed a couple fan leaves as well. She’s coming along nicely and buds seems to be forming nicely as well. Not really putting out much of a smell yet but hopefully she’ll starting frosting up soon so I get get a good smell from her. Day 62 - Not a whole lot new going on today. Removed a few dead fan leaves at the bottom and checked her out under a scope and looks like the trichs are starting to form. Day 63 - It’s the end of week 9 today and she’s drinking like crazy. Going to give her some more water and nutes tomorrow. She’s really budding up nicely and looking pretty damn good. The stretching has slowed down a little so hopefully she’ll start packing on some weight and getting frosty.

Una cepa muy fácil de cultivar, y muy agradecida de los cuidados y alimentos dados... En sus últimas 3 semanas engordaron muy bien llegando a formar muy lindos apicales centrales.

Pineapple Express was an exciting plant to grow. It grew fast and stretched nicely. Purple Kush was just a runt. It did not like the hot climates from the previous week but grew it out anyway. By weeks end was feeding Remo's full flower lineup. Pineapple - 8ml per 3800ml Ph between 6 - 6.1 Purple - 5ml per 2000ml Ph between 6 - 6.1 No change in light scheduled as with Auto's they do not require the 12 hour darkness period to flower. **Spider Farmer lights generate a lot of heat.

Día 69 (25/03) Su hermana Gorilla Cookies FF muestra una ligera necrosis... me preocupa... Día 70 (26/03) Necrosis en Gorilla Cookies muy avanzada en solo 2 días. Hablo con el gran @dogdoctorofficial y me hace ver que estoy regando mal... He dejado que se seque demasiado el sustrato! (como en la fase vegetativa) Y la Gorilla Cookies FF al ser la planta más grande y que más agua pide, está demasiado sedienta... Voy a modificar los patrones de riego para que el problema no persista en Gorilla Cookies FF ni aparezca en las otras plantas! Riego 0,5 Litro H20 sin nutrientes. TDS 225 PPMs - pH 6,6 Día 71 (27/03) Riego 1,25 Litro H20 + Wholly Base 3 ml/l + Solid Green 1,75 ml/l + Early Flower 0,75 ml/l + Big Bloom 1,5 ml/l de Gen1:11 TDS 1150 PPMs - pH 6,2 Día 72 (28/03) La necrosis en Gorilla Cookies FF se ha detenido 😁 y las flores siguen creciendo Observa alguna mancha de necrosis también en Wedding Cheesecake FF y decido a subir un poco la lámpara Ahora el PPFD en apical es de unos 830 PPFD Día 73 (29/03) Riego 0,5 Litro H20 sin nutrientes. TDS 225 PPMs - pH 6,6 Día 74 (30/03) Riego 1,25 Litro H20 + Wholly Base 3 ml/l + Solid Green 1,75 ml/l + Early Flower 0,75 ml/l + Big Bloom 1,5 ml/l de Gen1:11 TDS 1150 PPMs - pH 6,2 Día 75 (31/03) Detecto una ligera necrosis en Purple Lemonade FF y en Wedding Cheesecake FF. Voy a aumentar un poco la distancia de la lámpara para tener un DLI de 37, por si fuese estrés por luz. 💦Nutrients by Gen1:11 - www.genoneeleven.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

Ya ya baby these things are monsters!! Bent a few over they were asking for it LOL

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Today marks day 47. I ended up talking to The Green Sunshine Co. They agreed with me and thought I should top dress early, before the 30 day mark. I just did so yesterday, day 46. I think I have one photo from that day of the top dress. Anyway, I am concerned about the yellowing. She's way to early for that to happen in flower naturally. Hopefully the feeding takes care of it.

She seems to be doing a lot better since the foliar spray last week. Leaves are a dark green now and the stalks are more malleable due to the silica. Did another Silica foliar spray this week as well. 12 gal of water and it is supposed to rain on them today. Fingers crossed!

The central line was eaten by a caterpillar :/