This is my first induced flowering growth. I always grow weed outdoors with big satisfaction on yield and nature contact, But I have to say that training plants is real fun and I can’t wait to see the buds first appear and then fatten up. On day 5 of flowering she started to appear a little stressed, I think is a little overfeeding because her leaves are a little burned on the tip and a little underwater too but this morning end of day 6 appear to be recovering has the timelapse show on the end. Happy growing to you all brothers and sisters ☮️☮️☮️✌️🏼

Hello Growers and Tokers! 👋 👩‍🌾 🧑‍🌾.🔥💨 THE MOST PHOTOGENIC Thinking about changing the title of the diary to that.. 😂😂 I swear the camera loves this one.. And it's not the placement because I've tried moving them to that same spot and the pictures just don't come out the same. I guess the others just need to mature a bit more and they'll be more appealing in pictures because in person they really are.. 😍 Green sensation is really bringing it to the table. Buds are getting fat, stinky and sticky! I'll have to add a bit of support for sure because some are starting to bend a bit. Smell is just grrreat! She's definetly not ready yet so those 40-45 days of flower aren't that correct.. No problem with me though, i'm glad they've got more time to rippen up. Not much difference in nutrients.. I do use 2ml/L of enzymes every 10 days or so in flower.. Noticed it does great work in the roots during flower. Hope you enjoy the pics and vids. Taken on day 42 of flower. Check out the other strains i've got going as well. Take care out there! One love!

Hi all👨‍🌾👋 Welcome to my another week update Hope everyone keeping well 🧑‍🌾 Week 4 Feb 23 - Feb 29 Very good week. Girls are growing nicely. Finally a bit of work around my babies. On Feb 24 applied LST, girls responded perfectly. Leaves tucking on daily basis. 2 watering with 1L each on Feb 24th (half nutrients dose) and second one on Feb 28 alongside with 2 foliar feedings(100ml water and fish mix). So far this grow is just a pure joy. Both girls are strong and healthy. Wishing you all a wonderful week✨🍀 Much appreciate all your likes, follows and comments. 🙏💚❤️💜 Peace and love brothers and sisters 👨‍🌾✌️💚 Links https://2fast4buds.com/seeds/TROPICANA-COOKIES-AUTO https://www.biobizz.com/ https://fishheadfarms.com/

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Let’s Go Day 22 from seed for these 5 Ogreberry Autos from @twenty20mendocino and Bruce Banner from @fastbudsgenetics !! Today we turned the Scorpion up to full power and raised it to 38 1/2 inches above pots and getting a reading of 520 - 530 par ! gonna let the babies grow into the light now over the next few weeks till they hit into flower an keep a steady eye on it Incase I got to raise the light higher or lower !! Air temp an humidity has been great , stayin from 70-75 degrees through the day an humidity is staying around 58% ! So far so good Let’s grow lil Babies let’s grow!!! Hope you all enjoy an are ready for another amazing week ! Peace love an positive vibes to all you Growmies out there Cheers 😶‍🌫️💨💨💨💨💨🤙🏻keep them eyes peeled for next week y’all !! If there’s any questions please ask me ! Thank you all. 💚💯

So this lady has achieved such a beautiful shape and I think it's gonna beautiful to see her in full flower, very nice sativa leafs, fast growing plant that responds absolutely fantastic ti training methods and this lady has being flower on DECEMBER 22ND. So let's see how this beautiful bush of amnesia pro Performs!! Wish me luck Everybody!

Welcome growers I have finished the tirth week of vegetation with this young PINK KUSH 🌿 She's pretti healthy and faster in growing The lower internodes started to increase in size and they are looking for a lights ☀️ I hope to find some great colors with this one and some good Kush stone.. Trichomes city too ❄️❄️ Anyway.. In the beginning of the week I have transplanted into a 3l plastic pot.. Usually my choice of pot is the smartpot or the airpot 💯 And therefore, considering that the soil will obviously remain less aerated than usual, I have chosen to use a greater quantity of coco coir in the substrate I roughly prepared a mix of 50% light-mix (Canna professional and Biobizz), a 30% of coco coir (Atami), 10% worm-cast or humus (Atami) and around a 5% perlite wich it's already present in the light -mix.. I have also put a few micorizzae (Micosat F), like 10 grams, in direct contact with the roots and around 4 grams of Microlife (Bionova) in pellet form I will use this last one also for making an areated Tea, but during flowering.. About nutrients 👇 In this week i have giving their always organic/veganic nutrients.. Root-Juice (Biobizz) and Rizostimo (Biomagno) for the last time, plus a little bit of silicium (Bionova) and then Activator (Biomagno) / Crescita (Biomagno) / The Missing Link (Bionova) / X-Cell (Bionova) {This last two i have used in a foliar spray solution} Simply a little bit of everything at any watering 💧 About the lights 💡 I currently using for the first time, two Mars-Hydro SP3000, running at 25% of the power for now.. Next week im going to increase at least to 50% ⬆️ Onestly this lights looks pretty solid, definetly intense, with a spectrum really close ro the sun light One of the cool things about it it's that you can connected each one toghter with a cable and so you can controll the intensity of the lights very easy, in a precis way 👌 We will see in this months how they works And that's all.. See you next upgrade.. Thank you so much for stopping 😎👍 FC ✌️ 🇮🇹

This week I started her LST and bent her top over as far as possible without snapping and locked her in place. I use soft wire "Gear Ties" sold in the hardware section of most places. I got mine at Home Depot. I highly recommend these for anyone serious about Lst. I kept her in this position for about 4 days before adjusting placement and pulling the main top back down below the lower branches. I have been maintaining the same feeding schedule "week 4 hydro" 3/4 str main nutrients, 1/2 str Open Seasame. She started showing preflowers on me even though it's been in an 18/6 light cycle. So that's why OS and week 4 feeding was opted. Even though the plant was technically 2 months old, she's only been fed and treated right for 2 weeks now. So week 7 from seed, I started at the week 3 schedule.

One more week to go before transplant on site 3.

Very hot weather, girls are managing better than I thought. Cleaned up all the girls extra leaves, really needed to get some more airflow through it Cut out 2 small nugs that had a little bit of spiderweb mildew Done nutrients and done instant compost tea

Day 8 - A week since they poped & 4 plants are now fully viables , the 2 others aren't really strong so let's see if they survive. 6 plants out of 12 seeds : 50% germ rate Day 10 - They doing weel , only 1 is still weak but the 5 others are coming along nicely Day 12 - Leaves are bigger. , next week they'll go in their final pot 7L or 11L for a full Outdoor run. No feeding , just clear water Day 14 - They are now strong enough to be transplant in their final 7L pots. I'll also start my feeding schedule on day 15.

Flush is on!! Now we wait on a fade 😊

Blue dream. Je poznat ze o proti OG ztrácí tsk týden což je pořad super. Moc se těším až s ni začnu LST. JE to skvělá odrůda od Humbol. ❤️🌞

Today 7/30/19, day 57 and the start of week 9. I'm still continuing to alternate between days with my pesticide treatment (especially after finding that bug living on one of hulkberries fan leaf) and thrive alive green daily foliage spray as a prophylactic solution against any possible pests or mold going into these last few dog days of veg. I will flush them with distilled ph'd water for two days this weekend in preparation for flower. Then I'll start them on some "flower fuel" bloom booster on top of my secret for success flower recipe at 1/4 of the recommended strength. Outside of that still no adverse issue to report for the end of the 8th week. After my last trim, they both have responded very nicely, I was kind of worried about fat banana stalled growth. But one thing I noticed when I topped her at the 6th node, yes the main stem stopped growing but it developed many bud sites up and down every stem. Whereas hulkberry was topped at the 8th node, she has mainly focused on developing long stems and only 2 or 3 bud sites along her stems. Even though hulkberry in structure, is much bigger than fat banana and has a way bigger root system. Fat banana is bushier and has developed way more bud sites than hulkberry. I can't tell which strain will yield the most at this point only time will tell! Also, a big shout out to "J"@RQS, thank you for the excellent customer service. Another reason why Royal Queen Seeds is top 3 in breeding!!

ZAMMIGROW2024 Zamnesia provides monster hybrids so much fun growing no hiccups cants wait for these girls to fully bloom they smell amazing already pineapple express is the best ine there

Eccoci qui... Finalmente dopo uno stop per il lavoro torno ad aggiornare il diario con il capitolo finale... Personalmente una delle crescite più belle che hanno avuto le cime davvero da far venire l'acquolina in bocca e prenderle a morsi.. Grazie ad Anesia Seed e ad Xpertnutrients per la collab e a tutti per il supporto🔥🌲❤️ NE VERRANNO DELLE BELLE

Uma planta estava muito travada e pela minha experiência não vale a pena o custo da manutenção dessas plantinhas é chato mas preciso do espaço e do tempo para cultivar plantas que deem resultado. Coloquei um gancho leve em cada planta hoje para ver se aumenta a área verde.

One week and harvest big day Purple Punch Auto Barney's Farm. Grow Your Own! Love purps buds.. Thanks for watching.

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