got some Ph problems because i fckd up mixing water with BioBizz

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

All good

Alle 10 seeds sind gekommen und sehen soweit ganz gut aus außer eine von den Eldorado og die sieht leicht mutiert aus und häng auch im Vergleich zu den anderen hinter her bin aber im Großen und Ganzen zufrieden mit dem Wachstum !!

395g from 2 autoflowers and boy is it the FUNK. My house smells like the strongest OG kush imaginable from p2 and p1 is already starting to get extremely citrus smelling in its jars. Great job mephisto.

Buds are not as big as I'd hoped going into the 6th week of flowering. Not sure if it's got anything to do with it, but it's been quite warm this week. It's cooling down next few days so hopefully can get the temp back down below 30c with the lights on. A couple more of the lower leaves have yellowed and dropped off, and now there's a clear canopy area between the leaves at the base of the colas and medium, which should help with airflow. There's been no noticeable vertical growth beyond colas getting taller so I've dialed the biogrow back a bit more, and will probably stop using it all together from next week onwards.

great strain, it has an awesome scent and taste, average yield but really great buds! it was a pleasure to grow it, i harvested a bit early but due to bad weather and high RH i had to harvest

This grow cycle was one of my best from start to finish I had 5 females 4 of which were brilliant all shown colour all fruity gassy and amazing only the one was bland and green in colour and smelt different really good nug atructure but the smell wasn't there for me ... this is around my fifth grow and I'll be running these strains again to get it down to one pheno of these genetics

Everything going good. I only give the Holganix once a month

When i arrived i saw that lady twos cage had fell right over her... So some lower branches broke in half.. i tryed to fix it but whatever.. and i took away all the yellow leaves. Also added some organic biobizz nutrience. They r looking good flowering! Weak smell but smells wonderfull. Any tips or advices feel free to comment! Cheers homies!!

Maybe last week for Mary Jane. My first love I will continue to give her only RO water and ripen, maybe sometimes some flora bloom. Checking continuously to understand the best moment to harvest Any suggestion on that would be appreciated! Good Luck to myself @26/06 Added 2l with 1ml of PH+ EC: 1160 PH: 6,26 @27/06 Added 2l with 1ml of PH+ EC: 1177 PH: 6,18 @28/06 Added 2l with Ripen and flora bloom EC: 1330 PH: 6,1 @29/06 Added 2l of fresh water EC: 1210 PH: 5,5 @30/06 New res 10l with 15ml of Flash Clean for the final rush I'll harvest on friday.

Did grow a lot…i think these big pots allowed the plants to get this big

Day 43 12/08/24 Monday Feed today, it was warm again so they drank the whole 300ml each so I have topped up another 150ml to see run off to prevent salt build up. All thriving 😍✌️💚 ACOP and Melonade Runtz structure is stunning 💪 Divines Overdose is getting chunky and showing nice stack potential 👌 Leaving these to just run there natural course, will allow me to best pick my next SOG run with micro autos indoor 😎 Video update Day 45 14/08/24 Wednesday De-chlorinated tap water pH 6 only today. Day 48 17/08/24 Saturday Another watering today using de-chlorinated tap water pH 6 only. Orange bud is tall!! Top her next time. Critical orange is lovely and compact. Overdose is nice and compact, Melonade Runtz nice compact structure also. Picture and video update ✌️ 💚

Sono bellissime! Più o meno era questo il risultato che volevo, non so se raccolgo di nuovo 400 gr ma ci dovrei andare vicino, devo comunque migliorare la fase vegetativa, sono lontano dal mezzo kg che ho raccolto lo scorso anno. Ad ogni modo sono soddisfatto, i tricomi iniziano a diventare ambrati, soprattutto la pianta di sinistra, che è sicuramente più avanti di maturazione rispetto all'altra, come si può vedere anche dal colore delle foglie. La pianta più matura penso che la raccoglierò tra una settimana, l'altra vediamo. Nel frattempo ho di nuovo svuotato i vasi e messo acqua liscia con flawless finish, tra 3 giorni la cambio di nuovo e questa volta dovrebbe essere migliore di gusto rispetto all'ultima volta che era tanto bella ma un pochino forte. 🍀😎🎉🎊🍀🍀🍀

During my previous grow, I harvested and made some bubble hash. Afterward, I dropped the used, wet buds onto this coco, and it seems some seeds sprouted from the leftover material. I’m not even sure if they’re photoperiod or autoflower, but hey—let's roll with it! Both my pH meter and PPM meter are broken. I have no idea why they keep breaking so often. They’re ADWA meters, and while you can replace the reading elements, the replacements cost half the price of a new meter. It’s frustrating, and I really don’t want to keep buying them every time I start a new grow

Ooops I made some mistakes lol all gravy teachable moment …I really think I got a ghost in my grow op lol….anyone recommend an exorcist …im scared lol

Bravo, I am surprised by the fast buds 420, quality seems Both are 46 days old Gorilla Zkittlez Auto And Gorilla Cookies FF let's see what happens next week 🌲🌱💚🌵🌧️🌦️

Ginger Nut Cookies & Aussie Music Videos Fastbuds GSC x Barneys Red diesel 6 weeks of flowering completed 9th April 2020 Hi Everyone I hope you are all feeling well and keeping busy during the lockdown. It's raining cats and dogs here (lucky there's no chinese around LMFAO) and the humidity is 100% so today Ive chopped this little lady down and she is now drying up in my shed with fans going 24/7. I was quite pleased with the amount of bud I got off her and it's smelling fantastic and is very sticky.The trichomes were very milky with a few amber as well. 😍 very easy to grow no fuss and no problems but we'll have to wait and see as to whether she is a good smoke or not . Now for this weeks Aussie Music videos 👊 Because we are almost at the end of this grow I thought I had better add a few extra songs this week to make sure I get a decent selection of Diffrent music videos for you all to check out. So for this weeks selections I chose Empire of the Sun 👊 Walking on a Dream https://youtu.be/eimgRedLkkU Big Pig 👉 (from Bill n teds excellent adventure) 👊 I can't break away https://youtu.be/f_rfXVdYelA Peking duk 👊 Reprisal https://youtu.be/4GboBAg2Syo The white stripes 👊 Seven nation army https://youtu.be/0J2QdDbelmY Grinspoon 👊 Chemical Heart https://youtu.be/58mXxWuUqYw The hilltop hoods 👊 Leave my lonely https://youtu.be/yKifJ4Q5ph0 And Silverchair 👉Not bad for a bunch of 16yr olds which they were at the time this video was made https://www.youtube.com/watch?v=RZD982yrmx4 The Next update will be the Final harvest update with the dried n trimmed buds etc. I will also have to add another heap of tunes in that I had wanted to play , so keep an eye out for my next Update of Ginger Nut Cookies & Aussie Music videos .😀 Until then Stay safe and Keep lit And we'll all get thru this Together 🙏 👍