J'ai sorti la plante quand il y avait un rayon de soleil, sinon, elle passe le plus clair de son temps à l'intérieur. Je n'ai pas arrosé de la semaine. Elle avait été trempée avant par de fortes pluies. La plante a pris un coup de froid. Ça se voit sur certaines feuilles de la tête principale. Je fais attention maintenant de la rentrer le soir. Bonne semaine, dans l'ensemble.

Some of you may have wondered: Why do I use CO2 at average 950 ppfd μmol/m2/s (moral flow)? The answer is quite simple. Because of lack of space in some regions of my cultivation area, I simply cannot keep the ideal distance to my Sanlight high-performance lamp, due to some height growth of various strains. And so some of the main colas have ppfd values of 1250 μmol/m2/s and even more... So this is how I manage to achieve and compensate for such high radiation levels even with a CO 2 balance. And I have to say, my strategy to avoid various light stress symptoms works just fabulously. In combination with CO 2 implementation, my babies are simply unbeatably insensitive to light. Thats it! Beginning of 3rd week flowering: Again feeding my babies by 36 hours fermented potions of Bio Tabs Kompost Tea PK-Booster (15 g pro Liter) and added: 5ml Orgatrex/Liter 1 Spoon of Bactrex 1 Spoon of Mycotrex 1 Spoon of Mycco-Vital 1 Spoon of Dynomyco A little tip for those who are interested in small modifications that have a big difference or influence - on the result - effect - beauty - health - taste! Before adding microorganisms or beneficial bacteria or Mycorrizae and Trichodermas, please use oxygen-saturated water. On the one hand, unwanted chlorine gases evaporate and the small world of the microbiome becomes even faster and more rewarding in compost tea to sprout. Last but not least for this week, I would like to introduce my reasons, why I prefer growing biologically and sustainably. First of all, it’s something which suits very well in these times/days we are living now. Sustainability is a big need and task for our planet. 🌎 Nature means life. Our home, the air we breathe and everything that surrounds us. Not just today. Hopefully tomorrow as well. Maybe I'm starting to protect our environment on a small scale, but maybe I can also make a big difference at all. If you change the way you look at things, the things you look at change... I thought about what’s the difference, between Mineral Feeding and Super Soil Feeding. It’s very easy. Biologically Growing is a similar process than the natural soil activities out there in the lap of nature’s. So plants has to work and interact with the microorganisms and microbiomic communities in the soil. So the plants will never get lazy like the lazy ones of mineral feeding growers. If you grow biologically, you will feed the soil first and the microorganisms will support every parameter next to your plant conditions. And that will generate an unbelievable spectrum of Terpenes and Trichomes you will never forget. It’s the same comparison, when you daily visit McDonalds and you eat only fast food. How does your body and mind react on this shit for money?!?! May I invite you to think about it… See you next week dear Growmies! Have a nice Weekend and take care… Peace out! Addendum for Day 53: At the moment we unfortunately have another winter onset here in Germany. This means that I am forced to take additional heating measures due to structural facts in order to be able to keep the temperatures constant. After all, just tonight the thermometer climbs again to -1 degree Celsius. In addition, my exhaust air system runs out of my bedroom terrace and I therefore grow winter and summer with the patio door open. Well, sometimes I experience real weather-related challenges. But all in all, no problem... "Where there's a will, there's a way." Addendum to pouring out the fermented PK tea: I always administer half a liter of lukewarm aerated water with 3.5 ml of cannazyme per liter to each plant. This means that the "root machine" is not supplied with supplies unprepared and the nutrient solution can thus also be better distributed in the soil. Since I also work with cloth shoes, I spray them evenly moistened everywhere with water that is also warm before pouring them out from the outside. This has the advantage that the moisture stays where it should: in the pot! ... I did the math today ;-) We are still in week 7 until this Friday. And week 8 starts on Friday! OMG... still so much time yeahh! Today my Fast Buds Sour Jealousy and Sweet Seeds Big Devil and Dark Devil Automatics arrived. I'm looking forward to it. This time Fast Buds next Time Sweet Seeds. Love them too. Very beautiful genetics. Today a review video of the beginning of week 3. At the time of the pictures, I had minor signs of nitrogen excess. (Light peaks first at the crown of the roof and then slightly continuing to the middle section.) I then painstakingly racked my brains as to why this could be. I found that very slight dry spots had formed and therefore the root found small accumulations of nitrogen that caused its problems. But then, when I carefully homogeneously checked the moisture content in the substrate, the problem evaporated again. However, they had not shown any loss of growth rate during this time. Nevertheless, they developed as expected. They Strawnanas had no problem with that. In the end, I always have a hard time killing them. But I guess that's the way things go. We live and die. We come and go. But it's not there yet. ;-) Tomorrow is day 56. Tomorrow’s updating day! Can't wait to see their progress. Have a good time and see you tomorrow… 🏽🕊️ 🏽 ☮️🕊️

Exotic Genetix: Event Horizon (x3) y Toasted Toffee(x3). Thug pug Genetics: Peanut Butter sunset(x3). Una ves sean transplantadas a su macetero definitivo(5 litros) se comenzará con riego automático, drip to waste, y protocolo Crop Steering. 20 Octubre(15:30): 9 semillas en remojo, solución de 2 partes de agua de ósmosis + 1 parte de peróxido de hidrógeno 3%, 5.8pH, 0.6EC, 28°C~30°C. Antes ser puestas a remojar se lijaron las semillas para favorecer la absorción del agua. 21:30: puestas en toalla de papel con la misma agua de remojo, dentro de un contenedor de vidrio hermético y sobre una alfombra de calor a 28°C~30°C, para aumentar la actividad metabólica. 21 Octubre: Pre carga del coco, con solución de nutrientes Athena en 2.0EC y 5.7pH, saturación hasta lograr drenaje, el drenaje fue de 1.5EC y 5.6pH. 21:50: Todas las semillas presentan radicula de distintos tamaños(1cm aprox) son inmediatamente pasadas al sustrato. 22 Octubre: 21:10, se aprecian todas ya brotando, se mantiene el ambiente en 26°C~28°C y 70%+ de humedad. 23 Octubre: 9/9 semillas brotadas, se ven algunos Cotiledones. 24 Octubre: (9:30)8/9 cotiledones abiertos, altura entre 3cm~5cm, etapa de plantula iniciada. Toffee n3 se atrofió por mala manipulación, espero que crezca aunque sea lento, si no, tendré que seguir solo con 8 plantas. 25 Octubre: Toffee n3 está viva!!! Estuve a punto de quitarla, y está mañana apareció sin su capucha, cotiledones afuera. Yujuuuu!! 26 Octubre: hoy tuvieron su primer riego con nutrientes Athena, 2.0EC y 5.8pH. Se regó hasta alcanzar drenaje, el drenaje fue de 1.9EC y 5.7pH. 27 Octubre: Se desarrollan a buen ritmo, algunas han estirado más que otras, alturas entre 4cm~7cm. 31 Octubre: Segundo riego, 120ml casa una, 2.0EC y 5.8pH, runoff de 2.3EC y 5.7pH. 2 Noviembre: Creciendo a buen ritmo, alturas entre 5cm y 9cm. 4 Noviembre: Riego 180ml cada una, 2.0EC y 5.8pH, runoff 2.8EC y 5.8pH, desde ahora se regarán más seguido, removí las primeras hojas, solo por comodidad para regar, de igual manera los primeros nodos y esas hojas se terminan podando pronto. 8 Noviembre: Riego 2.0EC, 5.8pH, 240ml cada una para aumentar el runoff, valores de runoff de 3.0EC y 6.0pH. Alturas entre 9cm y 14cm.

4.7.25. What’s up growers! Just finished up with germination and seedling stage so here I am at vegetative! Plant is at 3rd internode in 1st week of veg so time to tie down method. Everything looking good so far! Only using about 1/2 strength of nutrients until an increase is needed! Thanks for checking out

Saudações Iniciar diários fast buds Encomenda chegou dentro do prazo, muito discreto. Dia 16/11/2023 Selecção de 5 sepas, colocando em papel toalha Dia 17/11/2023 Colocando em vaso final, com um cúpula para manter umidade alta. Agora é esperar que corra tudo bem Não se esqueça de ver novidades em https://2fast4buds.com

The buds smell a little like standard weed not so special but that doesn't surprise me as the plant looked in bad shape almost the whole grow. Exited to see some return on the hash, will be making fresh frozen so that should be cool.

Both Barbarian (=AK-47 x Barbara Bud) plants are filling up more and the first pistils start to get brown, which shows the plants start to ripen and go towards harvest time. The smell they exude is STRONG now and VERY PLEASANT. The aroma is sweet, peachy with sandalwood undertones and is MOUTH-WATERING. The plants are TRICHOME MONSTERS and are covered in crystals, any little touch and your fingers stay sticky for hours.

2 of them really starting to cannibalise all their fan leaves.. on day 56 and wanted to make day 70. Not sure if I should cut down a few now or let them all keep going til the end. Got the gnats under control. Days are going by very slowly 😆 3 different genres of music to go with each strain haha. Smells are out of this world when I unzip the tent! 24k and sour kosher all smelling gassy with lots of earthy undertones and the gelato just smells straight gasoline.

Week (19-5 to 25-5) 19-5 Temps: 19.5 to 25 degrees Humidity: 39% to 60% Watering: 1000 ml. 1.6 6.3 Light set from 80% to 90% strength. 20-5 Temps: 19.9 to 25.1 degrees Humidity: 37% to 60% Watering: 1000 ml. 0.5 6.4 21-5 Temps: 20 to 24.4 degrees Humidity: 39% to 60% Watering: 1000 ml. 1.6 6.3 22-5 Temps: 18 to 24.1 degrees Humidity: 38% to 55% Watering: 1000 ml. 0.5 6.3 23-5 Temps: 18.1 to 24.3 degrees Humidity: 41% to 57% Watering: 1000 ml. 1.6 6.3 24-5 Temps: 18.4 to 25.9 degrees Humidity: 46% to 57% Watering: 1000 ml. 0.5 6.5 25-5 Temps: 19.3 to 26 degrees Humidity: 43% to 61%

This week I had to Lollipop again (I know is very late and I hope I didn’t stressed the plant to much). My canopy is so tick and so many overlapping leaves and buds in the center of the plant that don’t receive enough light or any at all , n combination with the RH swings that I got going on (45 and 63 RH), I’m very afraid of mold. I should have did a better job at Lollipoping from day 21. I’m inexperienced grower and I didn’t knew better. I decided is better to risk to shock the plant a little bit risking a reduction in yield then get mold or pests and lose it all. My 30 pin dehumidifier works great with the AC but sometimes when the AC( compressor) stops my RH jump at 62% -63%, witch I don’t like . I’m trying to keep the humidity around 50% but in such a small room between the AC and Dehumidifier I got big swings in temp ( 75-82) and RH ( 45%-63%) witch is not ideal but is nothing that I can do. I played with the AC and Dehumidifier setting a lot trying to keep humidity around 50% and temp around 80 degrees and that’s the best I can. My AC is set at 82 degrees and my Dehumidifier is set at 45% RH. Also during the lights off (day time) is very hard to keep the temperature In control when outside is 110-115 degrees with my portable AC (1400BTU) in the 4x 6 closet. Is crazy but I do my best to keep temp under control. This is another rocky mistake I made is trying growing weed DWC in the middle of summer where temps are 105-115 degrees constant in a closet without centralized AC port and at second level in the building. LOL Also I think I underfed her (630 PPM TDS, PH 5.8-5.9) ) and I got a Ca deficiency witch I think is the reason my flowers developed slower then I anticipated. Hopefully I’m wrong and everything will be good. P.S. I got a little bit of calcium deficiency caused by salt buildup (to much PK boost) despite my earlier belief that is underfed. I increased the PPM from week 4 (630-640TDS) to 680 TDS) on week 5 while I kept the PK booster at 3+ml l per gallon. Slightly my PPM’s was rising in my reservoir for few days until I decided that my plant can’t take that much nutrients (PK). 2-3 days after I increased the TDS I’ve noticed more spots on the lower leaves with tip burns on top ( first signs of PK toxicity). One day later found spots and yellowing of the leaves on top of the plant that indicates Ca deficiency caused by salt buildup ( PK toxicity) and reversed osmosis (hope I’m not wrong). Going into week 6 I lowered the PK boost at 1.5ml / gallon and total TDS to 640 PPM. Seems like leaves damage to stop and my PPM to stay stabile at 630-640. Trying to keep PH at 5.8 max 5.9. I will post pictures of my Ca deficiency/ PK toxicity. If you guys have any suggestions please leave me a comment.

July 26: Lemon Cream Kush is doing just fine. Nicely recovered from me breaking one of the two main stems several weeks ago. I managed to tape it and she recovered quite well but it delayed things a bit and left her a bit asymmetric. Did a fourth lazy compost tea, see video explaining how i do it without an air pump and using cheap ingredients like blackstrap molasses and Epsom salts. July 29: defoliated lower fan leaves and small lower bud sites. Aug 1: added another round of malted barley and Power Bloom because the cannabis plants are showing signs of P deficiency. Blue-ish leaves and reddish petioles on another plant should be fixed by the malt and Power Bloom.

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 8 since time change to 12/12 h. The lady is doing very slowly this time. It's been like this since the veggie phase. I'll watch her for another week now and if she doesn't accelerate she'll be kicked out. But since it is so small, I can also let it run on the side of the tent as an experiment. Next week we'll see if it's still there 😅. until then have a lot of fun and stay healthy 🙏🏻 You can buy this Strain at : https://www.zamnesia.com/de/5165-zamnesia-seeds-kalini-asia-feminisiert.html Type: Kalini Asia ☝️🏼 Genetics: Black Domina x Purple Kush 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

📅 D119/D03 - 13/03/24 🚀 Drying 🎬 Added 22 pics and the timelapse of the harvest ________________________________ 📅 D120/D04 - 14/03/24 🚀 Drying ________________________________ 📅 D121/D05 - 15/03/24 🚀 Drying ________________________________ 📅 D122/D06 - 16/03/24 🚀 Drying. First trim : 3 g and a tiny flower placed in the jar for curing ________________________________ 📅 D123/D07 - 17/03/24 🚀 Drying ________________________________ 📅 D124/D08 - 18/03/24 🚀 Drying. I've made the very first try and she's very promising 😍😍😍 @Pure_Instinto_Seed_Bank ________________________________ 📅 D125/D09 - 19/03/24 🚀 Drying. Trimmed 7 grams and start curing of about 10 g for now. She's very sticky and very fragrant with an intense smell. Lot of rosin stucked to the scissor, good sign ! 😊 ________________________________ 📅 D126/D10 - 20/03/24 🚀 Drying. She's quite ready for trimming, I'll start tomorrow ________________________________ 📅 D127/D11 - 21/03/24 🚀 Drying. Trimmed 14 g from one of the main bud.... Less than expected but very dense, high quality buds ________________________________ 📅 D128/D12 - 22/03/24 🚀 Drying. Trimmed 11g ________________________________ 📅 D129/D13 - 23/03/24 🚀 Drying. Trimmed 22g ________________________________ 📅 D130/D14 - 24/03/24 🚀 Drying. Trimmed 11 g ________________________________ 📅 D131/D15 - 25/03/24 🚀 Drying. Trimmed 12 g ________________________________ 📅 D132/D16 - 26/03/24 🚀 Drying. Trimmed 16 g + 20 g + 16 g = 52 g. So far 132 g in total and 2 buds missing ________________________________ 📅 D133/C01 - 27/03/24 🚀 Trimmed last 15 g - Total outcome is 147 g. First day of curing

All going well topped four of them I think the wedding cake at the back left is doing the best💚👊

Week 13 (24-4 to 30-4) 24-4 Temperature: 23.8 degrees (lights on) 18.9 degrees (lights off) Humidity: 66% (highest) 53% (lowest) No pictures. Opened the reservoir for a couple of minutes. 25-4 Temperature: 24.7 degrees (lights on) 18.9 degrees (lights off) Humidity: 66% (highest) 52% (lowest) Increased the strength of the light from 60% to 65%. 26-4 Temperature: 25.2 degrees (lights on) 19.5 degrees (lights off) Humidity: 66% (highest) 51% (lowest) No pictures. I emptied the reservoir, there was 2750ml left. I made a 15L new feed and added it to the reservoir. Opened the reservoir for a couple of minutes. 27-4 Temperature: 26 degrees (lights on) 19.9 degrees (lights off) Humidity: 65% (highest) 47% (lowest) 28-4 Temperature: 26.4 degrees (lights on) 21.5 degrees (lights off) Humidity: 64% (highest) 47% (lowest) Opened the reservoir for a couple of minutes. 29-4 Temperature: 26.4 degrees (lights on) 20.6 degrees (lights off) Humidity: 64% (highest) 39% (lowest) Increased the strength of the light from 65% to 70% Opened the reservoir for a couple of minutes. 30-4 Temperature: 27.4 degrees (lights on) 21.1 degrees (lights off) Humidity: 62% (highest) 47% (lowest)

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

Switched the lights to 12/12.

Week 10 6th week of flower. Light settings 12/12 on at 9.00am off 9.00pm Light power= 75% 184w Radiator settings LIghts on Turn on if temp drops below 22.5c Turn off if temp goes above 22.5c Lights off Turn on if temp drops below 18.5c Turn off if temp goes above 18.5c Extractor settings temperature 23c with 1c steps Humidity 50% with 0% steps Max speed 10 Min speed 4 VPD aim 0.8-1.2 DLI aim 30 Wednesday 29/3/23 Day 64 (Day 36 flower) Nothing Thursday 30/3/23 Day 65 (Day 37 flower) Nothing Friday 31/3/23 Day 66 (Day 38 flower) Autofeed 5 15 minute runs Normal pump 4 drops ph d Feed 10L Ec=1.58 Ph=6.4 3L left Roughly 3.3L each. #2 Height= 51CM Dist=43CM DLI=26.4 Went away and left on autopilot Saturday 1/4/23 Day 67 (Day 39 flower) Away Sunday 2/4/23 Day 68 (Day 40 flower) Away Monday 3/4/23 Day 69 (Day 41 flower) Away Autofeed 4 15 minute runs Normal pump 5 drops ph d Water 30L Ec=0.22 Ph=6.3 Est. 24L left Roughly 2L each. Tuesday 4/4/23 Day 70 (Day 42 flower) Away I was only around for the 1st part of this weeks grow and most of that the tent was just running on autopilot. #2 Tropicana Cookies FF seemed to kick into another gear before I went away and looked like she may stretch a bit more. Power used for week Light (2.22+2.22+2.21+2.22+2.22+2.22+2.31)=15.62Kw Extractor (0.58+0.58+0.57+0.58+0.58+0.58+0.67)=4.14Kw Fans (0.97+0.96+0.98+0.98+0.97+0.99+1.01)=6.86Kw Dehumidifier (++++++)=Kw Radiator (1.41+0.67+0.92+3.24+2.08+2.71+2.69)=13.72Kw Water pump. (0+0+0.01+0+0+0.01+0)=0.02Kw Total for the week=40.36Kw Back in a week. Thanks for stopping by.

2 weeks left maybe 3

Off to a good start 2/3 seeds popped but starting another one and hopefully it’ll follow a week or so behind these two. Have a few purple gelatos flowering beside these a week or so out from harvest for and then I’ll be acclimating the room fully towards these two :)