Day 77. Harvest day for that amazing plant. Dry trim chosen. Will update after drying. Thank You Zamnesia for that chance ! Day 86. Girls has started curing process. Happy Growing !!!

Baby 8-11 week

Amazing how Criticical Orange Punch took some advantages of the nice weather. She s growing hard. Did some defoliation on her. She did an extra week in vegetation due to several defoliation sessions. Milky Way is doing great although she's not that tall as I was expecting. Smell is mix of lemon and mint with some spicy tones. Realy nice. 🤩 Some top layer for both with plagron bat guano. They had some treatment with compost tea last week. Now just watering every 2nd day.

The twin Organic grow has been harvested on 18.8.25. Here we about a week behind that one, as I did not feed well enough. Hopefully next week she will be done, but lets see! This is definitely the smaller plant. 17.8.25: I watered with 3L of dechlorinated water PH'd to 6.5 with; 💜 2ml Trace PH: 6.5 PPM: 355. Thanks for stopping by and hanging out 😁💚 ✌️ 🌱 🙏

The girls are fully in flower this week which of course is a GREAT sight to see when opening the tent. The smell in the tent is so sweet and fruity it's already making your mouth water!! So this week I feed the girls with a little of the living soil veg so they are still getting a helping hand. They were also given the living soil bloom and some barley which I will admit is the first time using it but so far me plus the girls are very much enjoying!! I have done the necessary light de-foliating on all 4 girls over the past 2 weeks and more LST. Happy toking guys and girls!! Will keep you all posted with more videos through the week so please keep checking back 🙂

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

She's still going! I have no idea what's going to happen, but I'll continue feeding her. She's nothing special to look at but that's fine. I was removing a withered fan leaf and I ripped off one of the flowers with it so I feel horrible. I put her under the roof in the Cure's place since that got chopped. I don't know what else to report. I'm still nursing her along, and I'll continue to do so until she says she's done.

With this beautiful lady just days away from hanging I must say she is a sight to behold I dont think my cam is doing the job right but I'm working on that for future grow. The buds are rock hard with a purple and gold color on her buds, she is already cleaning up awesomely so flushing should take it a notch up stay tuned.

Another good week, i love how red some buds get. Never saw some buds purple like that, but i hope they Taste like they look.

Прошла 6я неделя вегетации, пора переводить девочку на цветение. За все время вегетации пару раз возникали проблемные моменты с питанием, но все было достаточно быстро нейтрализовано, поэтому эта "вдова" к началу цветения выглядит превосходно!!!

Another good week!! I need to LST and defoliate... probably going to do that tomorrow. I fed them this week as well as watered. I will add the flowering nutrients going forward!! Otherwise, I hope this grow continues to go well!! Cheers!!

It was a good week one of my plants went into flowering at the last days of week 5 and she is showing pistils It wasent expected i thoght it vegetaites at least another week or so but we have diffrent phenoes and we should have diffrent expectations the other 2 are still in vegitaition They had a minor nitrogen toxicity i hadent grow automatic plants till now and i was giving them a little too much fertiliser bud i found out soon and flushed them so they are ok now Nothing too do more except doing LST evary day by pushing big branches away to expose the little ones to light and keeping the main stems and the little stems in same hight Thank you for your time and thanks to the growdiaries.com for letting us growers have this great experience together 💚 Keep growing🤘🏽👽

Big textwall incoming homies: Here Im doin some shit i knew from owning low to middle branded smd grow panels* how is that usually they come with both uv , ir, far red even newer quantum boards supplements itself with diverse chips extending their photosynthetic spectrum or making specific accesory bars / modules yet my purporse is rather focus on UV as this 15w bar (advertised as nominal 9 x 3w powered led) with UV-A 390-400nm spectrum – something that trigger some reaction to the plants with some other color son the flowering phase and trying to establish functional a ratio , as a bit of blue green and yellow have specific purposes instead of providing absurd quantities of red light , as there is some lateral cold bulbs in smaller but necessary ratios (as blue spectrum is still required on flowering , yet the original purpose was to complement mid to low branch development) in this shitty set up bonding with the upper bulbs and their colour temperature schema, every light has it's own schedule as how i suppose is a morning to night as something that cause plant resistance response (kinda noticed something i need to stimulate as i see a bit of fox-tailing but now with the uv-a i propose to build resistance and activate defense mechanisms like resin and of course, plant morphology according to its stage making bulkier , tankier , according to outdoor’s atmosphere i want to replicate as optimal weather as well – i hope this hardship make them more resilient and increase their health points as here i’s a HOT GIRL SUMMER- even indoors it rises my base temperature makes smell notorious, but as is not the usual weed smell make it and advantage in terms of discretion Killer Kush presents slight sweet tones not very prominent, the only herbal smell can be associated to pine undermining the refined coffee-like smell that is the dominant note (this selection of kush lack of lemon notes up to this point i can say it’s earthy with a bit of sour & sweetness) . Alas, as their late flowering phase and feeding schema should make chunky girls ,but the hot girl summer can become really a issue here, days and heat is something to be deal and prevent more light and food that they can chew , and this timing make me not over-do this niggas with excess of light, heat, disbalanced spectrum or extractions. So automatizing it with some timers ( a very good hint to repurpose old shit in your house!) im doing a Schedule that turn off some lights through the course of the day switching the intensity and the spectrum itself , making a sweet morning to afternoon look alike , , making that timer looks really like a basement like a nice dream to my plants, not exposing them to very disfavourable conditions and yet controlling my electric bill peaking , by the same reason , ventilation is also measured as im relying just on ventilators to distribute and disperse heat and also doing the role of intraction / extractors, there are 9 usb fans in various levels of this weed maze <3 . It's noticeable the quick evolution of these plants as there is no other factors to be aware except heat management as plants seem with not negative traces except some burnt leaf from previous weeks so i expect that they should be ready at the end of the month by my a personal mania of ripping shit all at once in order all to have that space at disposition for new projets , i will report stability eventually as they seemed quite rare enough when before putting to flower all, there was one already flowering AND THAT WAS FUCK THIS SHIT AND BECAUSE ITS NO CHEAP BULK TO EXPECT THIS SHIT!** ), and that shit become tears in the rain as that put me in some shit i can’t control or overcome, and just deal with in this strange lst bondage metal succubus nightmare that will be a nightmare to unmount too. Yet, i want the best conditions for her as all plants except her are ok so now the lateral bulbs also switched to give her more light (there is one on the left, right and on the back side, but i think better results with a neutral colour and using t5 led tubes with a water resistant case in each corner of the tent if i try to re do lateral lights ! ) *I still own the panels, in a next future, but not in this run i can use them as i think how i can supplement them and make them a team player as beyonding spectrum shaman shit i know now , and thankful to the chinese kids that made these fucking cheap as hell blessed bulbs,as they made me allow quickly adapt the spectrum on the main setup is 3000-4000k at the same ratio, with the addition of a cold light of 6500k on the idea of middle branching development too , and a small pint of unholy uv doing early 2000s cyberpunk shit with old trash janitor with compulsive hoarding stuff with some big gas leaks attitude niggas . **I do second runs of plants in order reach a better result through practice - but is noticeable the lack of stability on these bastards as i was very happy with previous strains i can say this strain is a big no! , unlike their famous ones, like black Jack or sweet cheese , the former ones fast version are way more stable and they didn’t presented me autoflowering phenos in the batch … i don’t hate autos, but i hate to mix them with feminized on the same run, its like having a hermaphrodite indoors for me. 😫😫😫

TThis week I decided to move from my small ass bedroom and pre fab me a area in my nice ass basement with great element and ceiling. I'll have a couple little videos. Still not done revamping that spot. But I'm hanging 3 mars hydro I just ordered from my beautiful rafters. Lay some mylar and a platform of a sort. Use some reflective tarp to outline my space. Switch out the basement lighting with 3 green led lightbulbs. 24/7 access to obsession. You're welcome. Still got the 4x4 pulled up there so everything is accessible. check in later

🍼Greenhouse Feeding BioGrow & Bio Bloom 🌱GARDEN OF GREEN SEEDS ⛺️MARSHYDRO The ⛺️ has a small door 🚪 on the sides which is useful for mid section groom room work. 🤩 ☀️ by VIPARSPECTRA (models: P2000 & XS 2000)

I'd like to say a big hello to all and thanks for taking a look at my first grow! all feedback is appreciated and I am happy to experience growing this plant even if I do not succeed this time. Apologies for the bad picture quality, I will improve this! without further ado lets give this Dwarf a happy life! Pre soaked the canna pro plus coco with plant start at 2ml/1ltr No activity from the plant in the first three days. using a big baggie as a humidity dome which has helped keep humidity high for the 🌱 Noticed on day 5 that the root has made its way through the soil bigtime and was desperately reaching for the light... immediately placed under the phlizon 3000w (630w true power draw) COB LED using only the LED supplemental lights (270w)and started a 2ml/ltr dose of plant start Day 6 - Plant has started to recover from being in the dark too long, starting rhizotonic today also at 2ml/l alongside 2ml/l vitalink plantstart. Regularly misting the humidity dome to keep humidity as near 70% as possible (sometimes with rhizotonic spray) Temperature is between 23c and 27c throughout the day. Day 7 - Nothing much to report other than healthy looking seedling, I've been alternating between the COB (330w) and LED (270w) lights throughout the day, have not to wanted to put them both on together incase I fry her :D Tomorrow I'm starting my week 2 and im seriously considering transplanting to my final pot (smart pot) which is a 5gallon/19liter pot... (will seek advice below) and switching from 2ml/ltr vitalink plantstart 2ml rhizotonic schedule to canna A +B 2ml/ltr canna rhizotonic 2ml/ltr.

End of week 10 and coming on well now smells starting to get strong now on these especially the papaya cookies she’s frosting well and the branches are starting to bend so will need to hold them up soon gorilla cookies #2 had some problems but hopefully will recover nicely onto next week and happy growing peace

I tend to forget about her as im mesmerized by others, she’s the one with the best perfume currently in the room. It’s very possible she starts busting late flower, trellis is up show me what you got girl. Coated top down she is some sticky icky oooooh weee! Yep hydro still in Veg Ive topped for a second round shit will be bonkers and going in the flower tent after this round. Will most likely initiate flowering in aboit 2 weeks or until she blows the top off this tent. Stay Safe Stay Fresh GHL

cloudy week grew 7.cm and looks healthy but slow ... the temperature has dropped ... I think she will be even slower outdoor ... .

This is a super strain no hesitation go get this one you won't regret