Weekly update for these lovely ladies. They've grown and grown since the last update so much I had to raise the greenhouse. Cinder blocks under the frame gave me an extra 16in. They're growing probably a foot of week and still reaching for the stars. I did switch to Athena's Bloom A & B and P&K booster this week giving 5-10ml per gallon. I really couldn't be happier with how they've grown up to this point. All in all Happy Growing

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Noticing trichomes more than ever. From what I’ve read the last two weeks are when buds start to swell up. Let’s hope that’s the case lol I really pushed the ppm’s this week only because I accidentally let her go over 1000 ppm’s during this week. I would have never have done that but she let me know she was ready for a full dose of nutes. Today is D81 and I’m expecting she will go over one hundred days. We shall see... thanks for listening!

Could have done her better.

Helloing 👋🏻friends and visitors. Welcome to my Pro-Mix HP+ Open Top Grow Bag QCS - Auto Fat Bastard grow diary. Feeding: Sun 5thNov: 2L with nutrients ph'd 6.25 Wed 8thNov:3L with nutrients ph'd 6.23 ---------------------////////------------- Always make sure to give a feeding of water only, once a week.😉 ---------------------///////------------- Did some defoliation of bigger fan leaves here and there, but probably will cut off some branches of the lowest shoots ..🤔 we'll see. Other than that it was a smooth sailing week. Increased her feeding from 2L to 3L and she loved it. next day her leaves were all spiky pointing up ☝️🏻 all happy to be🙃. This is it for this weekly update folks! Thanks for stopping by. Likes and comments are welcome and appreciated 😉 Keep on growin! Keep on tokin!!! 😙💨💨💨💨💨

Week 2. Just the normal stretch. Nothing eventful. After this week, I'll be giving random weekly updates due to my busy schedule.

Starting of the stretch getting way too tall lol

True Hash plant structure from the moonshine and flowering vigour of the Bruce are just KILLIN it. The terpene profiles on the parents are fairly similar so it should blend out to something unique. Seems like a daily battle between fruity and just a funky dank skunk smellwise. Some paleness and light chlorosis of bottom leaves persuaded application of Terp Tea. Soil conditions seem optimal since she was healing within a day.

This is the start of flowering! I started 5 days ago so we should see some bud formation really soon done some heavy training on GG#1 . Waiting on GG#2 to shoot out abit more then i will start the sliding stake technique on her as well . Waiting on the more serious formation of flowers before i give a good PK spike . Other then that things are looking good in the garden and we are well on our way to the finish line . Thanks everyone for your support keep rocking out! -Happy Growing!

Lets see

Switched from 24/0 to 12/12 at the end of day 14, also switched the 730nm Led's on. Its been 7 days the plants have started growing a lot faster but there's still no pistils showing. I started feeding biobizz grow @ 2ml/l At the moment there getting watered every 3-4 days. The light is showing 25000lux on a lux meter app.

I don't have single complaint. I love this strain and will absolutely be growing more in the future! Great bud structure, never a yellow leaf, just overall badass plant. Very happy I took a clone lol.

Took the ScrOG net out since I had messed up and let the plants get too bushy. Maybe try a full ScrOg next time. This time I think we’ll finish without it. Defoliated a little bit last week and took it slow. Tent smells strong this week when opened, very thankful for the carbon filter in the tent. Hopefully I get better bud development in the coming weeks. Currently watering each plant about 1/2 gallon every 24-36 hours each. Flushing this week then adding new ferts and lowering dosages.

Siguen estirando y formando muchos brotes gordos!!

It was a good run considering how it started. I wish I put her in a bigger pot and vegged her for maybe 2 more weeks and I would of ended up with another ounce. I also think I defoliated a lttle too much to early in fear buds werent getting enough light. Jusst under an oz is fine. Im doing a 2 week and 1 month cure so I will split it in half and update later on.

Week 4 day 1: Happy 4th! 7/4/25 Fed with nutrients : 7/7/25

Great strain. Will post more pictures as it cures and update the smoke report :) Going to aim for a 30 day cure and I’ll post how it looks

400W LUCALOX HPS + 100W DIY purple led