Well one just wants to keep growing and the other one wants to keep bishing out so it’s going to be a bush grow going forward. Cheers to good weed and good friends 🍻🍾

Been a good week I have lowered nutes as on some of the plants the tips have gone a bright yellow type colour wasn’t sure wether this was because of my lights or nutes either way I have lowered nutes for the minute and will see how that goes , went with another trim during the week as some flowers have grown far beyond The others main aim was to free up light for the lower plants and aim for there bud sight it’s seemed to have worked so far as they have grew a little other than that everything else has been quite manageable 🤞🏾 Obviously missed a day which was watering day due to other responsibilities but hey it happens on the brighter side The jungle is still slowly coming along nicely happy growing 🤑

Hi Everyone ! Everything is ok. Plant are still healthy. specially the tank on the left, they drink 15L during this week and maybe less than 10L for the tank on the right Guess which one has a nice root system ? 🙏😉

I think they got one more week left

Bella bella sativa

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Heute beginnt die 3. Woche der kleinen 3 Z-Up's von FastBuds. 💚 Die Ladies waren inzwischen bei Ihrem ersten Friseurtermin und wurden getoppt. ✂️ Sie haben sich seitdem bereits gut erholt und wachsen bereits wieder. Ich freue mich bereits sehr auf die kommenden Wochen. Genießt euren Sonntag. 🌤️

☘️17/11 - La semilla estuvo por 24hs en agua para hidratarse. ☘️18/11 - Puse a germinar la semilla en papel humedo, el 21 de noviembre germino, aprox tenia 3cm. ☘️25//11 - Ya salió el plantin. ☘️27/11 - Ya tiene 3 días de vida, se encuentra perfecta. ☘️Voy a dejarla una semana en Enraizamiento, asi las raices se desarrollan un poco mas antes de pasarla a una maceta de 10L. ☘️Los productos que voy a estar utilizando en todo el cultivo son los de Advance Nutrients. ☘️En crecimiento voy a dejarla 3 semanas con 18/6 de luz. Una vez que ya entre en flora la luz la cambio a 12/12 y el banco de semillas recomienda hasta 9 semanas de floración. ☘️Voy a estar regando con un ph de 5.8 hasta llegar a 6.4. ☘️En estos días estaré publicando mas imágenes de como viene. ☘️Podes seguirme en Instagram como @bruweed_arg para mas contenido.

Day 100 Day 57 Flower 06/07/24 Saturday Flushed with de-chlorinated tap water today pH 6.0. Flushing using 10L of water with Flawless finish- I'll be using this every other flush. , getting 50% run off, I can't measure ppm, I have ordered the tool to start learning this way also. I haven't measured run off ppms before so will be interesting to see. Smelling pungent, deep greens of colour coming through, hoping over the next 2 weeks she swells as she is not as dense as I was hoping 😅 Day 10day 58 Flower 07/07/24 Sunday No feed /water today letting her dry off a little more. Updated with a short 🤩💚 Day 102 Day 59 Flower 08/07/24 Monday De-chlorinated tap Water 5L pH 6.0 + 3L Water +calmag 5ml to keep her from deficiency during 1st week of flush. Updated a short video 😁 She's getting there. Day 103 Day 60 Flower 09/07/24 Tuesday No water, letting her dry off again ABIT, noticing a huge amount of trichome production, updated a short to see 😍. Day 104 Day 61 Flower 10/07/24 Wednesday Another 10L flush today , de-chlorinated tap water pH 6.0 with flawless finish. 10ml flawless finish to 10L water. Updated pictures and video. Day 105 Day 62 Flower 11/07/24 Thursday Last day of the week, another big flush today! 10L de-chlorinated tap water pH 6.0 with 10ml Flawless finish 👌 Trichomes...😍 She maybe a 21 day flush we'll see how she goes next week with ripening. Video updated.

Feb 22 This guy really is FAST. I guess FastBuds was right. I'll need to attach a timelapse to show just how fast it's taken off.

So these girls are now 11 weeks old, and they are well through their flowering now. Buds are starting to swell and mature (for Red Devil and Pineapple Express more than Misty Mack), and their smells are pretty powerful. The end is approaching, and as a guideline my plan is to harvest Pineapple Express @ 13 weeks Red Devil @ 14 weeks Misty Mack @ 15.5 weeks So that means Pineapple Express' two week flush begins today.

Day 96, 15th of December 2020: Wao and wao... this is what I can say. A bit late I was on holiday I usually start the week with the first day but now with the last one. So let's start with the "black sheep" of this group as she is alone here RQS Wedding Gelato. Easy growing issue free little bushy lady. Absolutely great choice of grow. Pretty much ready for Christmas but she can be cut even now lol.... And now let's speak about the Original Sensible Seeds' girls... Well Black Ghost OG similarly to RQS Wedding Gelato is calm little bushy and issue free lady.... no problem all good ready for Christmas.... Runtz Gum the thick chick with heavy big buds and excellent appearance is really amazing. I placed a stick there and fixed her to look better... It was not necessarily but i have done it. She needs more time pretty much done ny Christmas or a bit after.... Gelato from Original Sensible Seeds is similar in terms of acting such as streching and all that ... The same situation applies not much to really say we wait ;) I am really happy to have these ladies.... Well, Do-Si-Dos OG is the craziest one ever... lol.... not only due her crazy kind of streching style but the trichome developement that comes along to the fan leaf "holder" stem is pretty crazy.... Those buds are like a diamonds sticky and she would be ready to harvest but need to wait because I need to go.... I really had to fix her by placing a stick there I broke one stem but fixed as it did not come off... so crazy especially now because of the weight of the buds... now she looks even more beautiful... I think she is the most challenging grow ever. Original Sensible Seeds genetics is unique really high quality just look at them. Royal Queen Seeds as well but this is my second grow from them my first was a Green Gelato which is the same calm littlw bushy plant. Amazing very good for newbies ;) I am going on holiday again and back on 28th of December so after harvest starts. Girls will be cut after. The 7th weeks starts with tomorrow 16th of December.... Only flushing and no more nutritient will be used. Soon full report comes. Lamp is the same 11 hours on and 13 off.... The last 4 days they will get 4 days darkness and only ventilation will be working no even more water ;)

The weather's finally starting to warm up. It feels so good to have the sun out and to open the windows. With all this extra sunshine, my pound cake plant is really taking off! It's growing so fast, and it's starting to look really full and healthy. I can almost see tiny buds on the ends of the branches - maybe it'll even have flowers soon!

WEEK 31 March-7 April. I just finished all setup afteer transplantation and so far all is working fine. Now she is comfortable and has plenty of space. I'm very proud of my AQUALOOP homemade system, that works perfectly. I'm going to increase nutes until 1.8 / 2.0 EC and go to make her blooming in a couple of weeks. Update 09/04: I've made a big defolation and strong LST. I decided to start soon the blooming stage, maybe during the WE.

🍪🥜🍬⛽️

Harvest time has come for my first lady which was a little faster, and had a completely other aspect than ther other 2. A little purple color and shorter. Also got some issues during veg phase with her. Anyway got 428g nice good smelling wet buds from her an they are drying now. Will post pictures from harvest next week. All of them are cherry cola. The remaining 2 ladies look very promising and will be harvested and of this week. Today 70 days from seed.

Have grown 3 gorilla cookies autos out to harvest and all three have been noticeably different in growth and characteristics

La plante grandit de jour en jour et ses bud commence a faire des superbes filament orangé ,aucune carence pour le moment... wait and see