The Grow Awards 2026 🏆
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@mulch
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8/4/17 Calling this Flower but still feeding late veg nutes until stretch is over. Feeding every other day is indeed a labor savor, just over 30 minutes to water and vacuum as long as everything is pre PHed and prepared. I bought a pressure sprayer to more evenly water, but it won't work as it took about 20 minutes to water 1 plant with 1.5 gallon.s Not efficient enough, sadly. Lights are moving up once or twice a day. The time lapse camera will have to be adjusted as the tent is becoming overgrown. We have pre flowers everywhere, and Kush is 1st to flower. A couple other flowers forming, but oddly not any on the auto speed buds. I bought a dehumidifier to deal with lights out and post watering humidity spikes, and it said it had a pump so I could pump the water into a bucket outside the tent, but it apparently is just gravity. Between all my gear and the newly expanding canopy, it is starting to get crowded in the tent. I may have to return it, but I really don't want to as the size has it fit right where I need it. I think I found out what was eating my plant leaves. It was me. I accidently hit a leaf on Reek'N today with the shop vac while I was cleaning the runoff and it tore the end off a leaf and looked identical to the damage seen last week. On 1 hand, this is good news as we are still pest free (knock on wood), but on the other hand, I was careless with my girls. Shame on me. I will be much more careful in the future. I( am noticing what might be nute burn on some leaf tips on a few plants. I have not increased the PPMs since going to an every other day feeding, and am getting consistently more runoff than I was getting, PPMs of runoff are mostly around under 200 more than I am putting in, so the frequency must be burning them. I may back off one day and see what that does and adjust the schedule as needed. I could use the break, too ;) As long as they are stretching, they get Veg nutes, then a feeding or 2 of transition, then start flower nutes. Getting nute burn prior to changing PPMs was probably a good thing to alert me to back off the frequency of the feeding a bit, since they liked this PPM before with no complaints when feedings were spaced further apart. 8/5/17 They did not feed today as I am still monitoring the minor nute burn. I am starting to think, since I did not raise my nutes, that this nute burn is the result of me slowly lowering humidity to reach flowering VPD, in which case, the lower RH is causing the plants to transpire more and take up more nutes, hence the very minor nute burn I am seeing. I didn't consider the humidity to be a variable until a few minutes ago, but that would likely explain the issue. If so, I am pleased I got so close to optimal nutes that a variation of 5-10% less RH would cause this and concerned with recalibrating feed to this. It's a learning experience, tho, fo sho. They are still happily growing tho, I have had to raise my lights twice in the last 24 hours and anticipate moving them up once more today, so I guess I am still mostly giving them what they want. The Kush is the tallest at just over 22 inches. The Mango Cream, the shortest at just over 14 inches. I may have to destroy time lapse continuity to better match heights with lights as in full flower, they move to around 12 inches (they have been at 18 inches through veg). I likely won't be too aggressive on light placement both for spread and to avoid light burn in flower. We shall see. 8/6/17 LMFAO I am starting to look for a home for 2 of the Auto Speed Buds. Nothing against them in particular, but I may need to make room and I have 3 of them and only 1 of every other strain. Things are going a bit bonkers in the tent and unless I want to see them fight to the death, I may have to make room. I will monitor things and decide. What I thought was burn might actually be a def. I have consulted on a forum and have been persuaded to up my ppm at the same ratios. It makes more sense than burn, tbh, so I am going to go for it, new measurements to come, first feeding with new ppm tomorrow. Going to go 50% stronger in late stage veg and see how they respond, and perhaps will pick up the every other day feeding again. 8/7/17 New feeding based on slight def, full strength GH base nutes, EC 1.6, PH with white vinegar to 5.7 FloraMicro 5 ML gal FloraGro 5 ML gal FloraBloom 2.5 ML gal CalMagic 2.5 ML Gal Armor SI 1 ML gal Roots Excelerator 0.5 ML Gal Drip Clean .5 ML Gal Photosynthesis Plus 3 ML gal SM-90 2.5 ML gal Ful-Potential 2.5 ML gal FloraBlend 2.5 ML gal Floralicious 1ML gal 1.79 EC after nutes and additives cross your fingers that it is not too hot Soon move to transition nutes, drop Roots Excelerator and add FloraNectar So the plants were drier than I anticipated and the PPMs coming out were LESS than going in! I think the bump in nutrients was a good idea. We will see how these five handle it. 8/8/17 Just fed the other 5 full nutes, no trace of burn on yesterday's group. Light still going up twice a day. I am finding it very difficult to water and handle the Auto Speed Bud #1 in the center. The canopy is QUITE thick. The Kush is now over 10 cm taller than the breeder estimated for an indoor at over 70 cm. I am going to continue to feed every other day until they force me to feed every day. I am also going to continue with Veg nutes next feeding. 8/9/17 still growing, keeping the new full dose feeding. Will likely start upping the GH additives too. Kush is now over 80 cm. 8/10/17 As we wrap up week 6, Kush is getting close to 90cm, 50% taller than expected. 2 of the SPeed Buds are still in preflower, oddly. Everyone is healthy and getting huge. Having issues watering the plants in the middle because of the thickness of the canopy. Last night my Raspberry Pi got into a boot loop. Since I run the time lapse with that, I have to do an emergency new setup to get the time lapse rolling again. I am putting together a tutorial to make one yourself and will have info when it is completed. There will be some disruption to the time lapse from thi missing time period, but honestly, I have been having to move the camera up so often because of the growth it will likely not matter much. The plants are so big, I may push the next feeding past GH recommendations. Most people advise not to go anywhere near that, but the plants are so big and thriving so well and already got a bit of a def, I want to see if I can push them a little more by late flower. I have given full strength nutes and GH additives today to 5 of them, so will monitor and likely increase base trio ppms if all goes well.
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@mulch
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8/30/17 It's been an emotional week, sorry for the lack of updates. I finally had to admit that 4 of my plants, while fully pre-flowered, did not auto-flower under 20 hour lights. The decision was made to remove them from the tent. It was a tough decision made easier as Auto Speed Bud #3 reached over 130cm and was touching the light, blocking light from plants that WERE flowering. There was really no choice at that point. So I spent 2 days trying to find them a new home. Yes, I had grown attached. Yes I found them a home. They have been transferred to soil and will be going under 12/12. I don't really know the guy well who ended up with them (a friend of a friend), so I don't know if I will ever know how they did, but they were part of my first grow so I am glad they got adopted rather than me having to kill them. In other news, I have had to start adding stakes to keep buds from falling, notably on the Kush (my superstar and only LST plant) and the Mango Cream, which seems to have hollow stems. I was concerned about this until I read up on it and hollow stems are a sign of no lockout or deficiencies and good nute uptake. They certainly look beautiful, so this is good news. However, so far 3 stakes have been added to the Mango Cream, so I hope I didn't damage the roots too much. I am looking into an alternative to stakes for the next go round. I didn't expect buds that heavy, but I ain't complaining! Slight Changes in nutes: Current feeding FloraMicro 4ml gal FloraGrow 1.25ml gal FloraBloom 8ml gal SM-90 2.5ml gal Cannazym 2.5ml Gal Ful-Potential 2.5ml gal Liquid Koolbloom 2ml gal Floralicious Plus 1ml gal ArmorSI 1.5ml gal CaliMagic 3.75ml gal (still running it high, but after seeing water report, I might have been wrong to use GH hardwater micro) FloraNectar 5ml gal Drip Clean 0.4ml gal Flora blend is out this week, Grow is out next week. Switching from Ful-Potential to Ful-Power when I soon finish the bottle. Nothing wrong with Ful-Potential, I just keep hearing about how good the chelation in the Ful-Power is, so I am game to try it since I need more Humics/Fulvics anyway. It is worth noting that I am now running ALL the GH nutes at full strength with no hint of burn. I am tempted to push them, but being my first grow, I don't think I will. However, this is a solid foundation for me to tinker with formulas for my next grows. The medium and my setup has performed wonderfully so far, and if the harvest is a success, I will certainly be more confident to experiment further. I may, however, use full strength Ful-Power which recommends reducing nutes by 10-20%. Instead of increasing nutes, full strength Ful-Power may give me the boost I am looking for while minimizing burn potential. I love that coco helps buffer and flush things, by nature, that the plant isn't using. I am so stoked that I ended up choosing coco for my 1st grow, but that is another topic for another day! I have edited the diary to reflect the loss of 2 strains and the title to reflect the current reality. Perhaps I should have left them unchanged. I am not sure. However, the diary title and strains are correct at this point. So there it is. I am now attempting to feed all 6 plants every day. There will be some days that I miss, but so far my 10 gallon bucket produces enough runoff on 6 plants if watered daily and most people who end up adopting this late stage strategy often would double/quadruple it if they could spare the time and/or afford the nutes. Once a day seems a good enough way to take advantage of the benefits of the medium without diminishing returns. I wish that I had the type of life that allowed me to set up proper control groups to test this stufff. I have also permanently started emptying the dehumidifier into the nute bucket as it has a PH of around 5.3 (my tap is around 8) and a ppm of around 8 (my ppm varies from 150-200 since i have been monitoring), 1-2 gallons a day to help out. I am loving it as much as PH down with white vinegar. I also obtained a water quality report and found that although my agency adds chlorine, they do not add chloramine so airing the bucket out for 14-16 hours after filling from the tap and cutting it with dehumidifier water should remove any threat to the beneficial living things that I have added to my girlies, so this is good news. 8/31/17 Man, I wish I used stakes from day 1. Every additional stake I put in makes me cringe and more stakes are becoming necessary. I am just lucky they are so healthy.
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Dane, We all should help one another. Human beings are like that. We should live by each other’s happiness - not by each other’s misery. We don’t want to hate and despise one another, share the Joint. And mother earth is rich and can provide for everyone. We can Grow enough Happiness, In this paradise, there is room for everyone. We only exist to bring joy into the world and The way of life can be free and beautiful, but we have lost the way. Grow High and Give the world A smile. At the end we own nothing more then all our memories, lets make them amazing for everyone, nothing to loose only everything to win. A last kiss goodby, a second one, softer and long as a sign, that you are woth it. That Everyone worth who loved and give. Enought Hippie Talk, now have a nice day and an even better grow, thx for watching by.
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Buenas a tod@s... Otra semanita de floración para estas hermosas.... Han crecido bastante, las veo muy bien, riengo generalmente cada 2 días, el pH perfecto, absorben muy bien los nutrientes, el sustrato muy bien desde q lo eh cambiado este cultivo... Esty muy contento y el resultado va tomando forma, trabajando duro se obtienen buenos resultados a la larga, hay algunos fallos para mejorar pero de momento las niñas están bien y van creciendo y engordando favorablemente... Me gustaría tener más espacio y más material tecnológico para el cultivo, pero de momento es lo q hay y nos apañamos bien... Buenos humos para todos 🏻💨💨 😎💀 🇦🇷🤝🏻🇪🇦
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Hey hey growmieezzz ! It’s time to burry this RS11 🌈 🍦 into a bigger pot : this 7gallon (28L) smartpot will be her last home! I’m back to an old mix a did few times and it was best results of my runs : - Pachamama soil - Batmix @plagron_official 🦇 - worms Humus @biobizzwwo Last dayyyy! I replace spiderfarmer sf1000 by 2x mars hydro sp150 ! I’ll wait a little bit the root get stronger and I’ll turn it into flower next week! If the height explode, I’ll turn her sooner, but for the moment, I control the size !
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Day 17 17/07/24 Wednesday De-chlorinated tap water pH 6 with calmag 5ml -5L. Very humid week, noticed a green turning colour on top of soils where the humidity has been far to high. I have installed door netting and recently left open all day and night now so they can condition over night in colder temps. Growth wise, explosive little seed 👌💚 Day 19 19/07/24 Friday Lite Feed today, 250ml each pot small run off. Seeing excellent start to these babies. Let's get it 👌💚 Day 21 21/07/24 Sunday De-chlorinated tap water pH 6 with calmag 5ml to 5L. Watering in 1L each day from now. Updated video
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ONLY 3 PLANTS LEFT IN THE 8X8. LESS THAN A WEEK EVERYTHING WILL BE DONE 🤞 ALL THE PHOTOPERIODS ARE IN THE 8X8 NOW. THE STRIVE NUTRIENTS IS INSANE.. BEST FERTILIZER PRODUCTS EVER MADE. I USED IT 4 TIMES IN 13 DAYS AT FULL STRENGTH AND THERE IS NO NUTRIENT BURN AT ALL AND THEY EXPLODED. AND I HAD A FEW THAT WERE TURNING YELLOW, I THOUGHT IT WAS ROOTBOUND OR CALMAG DEFICIENCY BUT THE STRIVE NUTRIENTS BROUGHT THEM BACK AND THEY ARE SUPER HEALTHY AGAIN.... ATTENTION GROWERS:::::: YOU MUST TRY STRIVE NUTRIENTS. BY FAR THE BEST FERTILIZER PRODUCTS EVER.. I PROMISE YOU THAT AND IF YOU USE IT YOU WILL NEVER USE ANYTHING ELSE FOR EVERYTHING MARS HYDRO VISIT: www.mars-hydro.com www.marshydroled.ca PROMO CODE: rocknroll VISIT THE ALIEXPRESS STORE: @marshydro_aliexpress Instagram: @marshydropenny https://youtube.com/channel/UChU5ZWwNi-DvnO3oWVU3ceA (NEW) STRIVE NUTRIENTS 💪 Thanks for taking the time to read and check out my diary🤜🤛 FC-E6500 LED GROW LIGHT: FC-E6500 grow light with 3978pcs OSRAM BridgeLux Diodes & Moso Driver, market-leading PPF 2.7 µmol/J, Max 2.5g yield/watt with 30% higher Average PPE, more cost-effective when compared to same style type lights. Only consumes 650W, perfectly cover 5x5ft (personal), 4x4ft (commercial) grow space. Innovative Precise Uniform Lighting-Built-in removable bars interval & dimmability enable the exact & dynamic PPFD control over each of the plant growing stages, FC-E6500 detachable bars deliverSuperior Heat Dissipation: Real Aluminum heatsink together with 6 slim profile passive cooled bars (THINNER & LARGER surface) and detachable Meanwell Driver, create less heat. save 50% hydro than other old grow lights! Driver can be mounted remotely on the side of a grow tent/rack or on an adjacent wall to remove heat from grow space. Full Spectrum UV IR LED: With Yield Max spectrum (660nm Osram enhanced Deep Red,2800k-5000k,730-740nm IR,380-410nm UV LEDs), Average PPFD the FC-E6500 LED grow lamp enables cultivators to produce BIG FAT Dense Buds, perfect for full-cycle hydroponic, soil, grow tent/grow room. Dimmable Commercial Grow Light: Cost-efficient, easy dimming & daisy chain With a max up to 20 lights, IP65 Waterproof, master light easily controls the rest. Perfect for commercial & home horticulture. FC4800 LED GROW LIGHT: GET THE MAX FROM MARS👍: Welcome one of  the newest members of the Mars Hydro family🤜🤛🤘. The Mars Hydro FC 4800 480W LED Grow Light can accommodate up to a 4' x 4' area for flowering. It has OVER 2000 LED chips consisting of the best selling Samsung LM301B diodes which has a Luminous Efficacy of: 220 lm/W 0.2 W, 3 V mid power, Osram 660nm Diodes, Infrared light and UV LIGHT👍. It boasts the incredible efficiency of 2.8 µmol/J, maximum 2.5gram yield per watt with 30% higher Average PPFD. This High-intensity spectrum is beneficial for plants at all stages - from veg to bloom, and creates Big dense Buds👍. achieve 50% higher yields & quality compared with the old lights! Only consumes 480W for 4x4ft personal grows, and 3x3ft for commerical grow. It comes with a dimmer and daisy chain function with a  maximum of up to 15 lights, the master light controls the rest👍. Wide 6-bar design offers even & wide canopy coverage and can maximizes your grow space. slim 180° FOLDABLE DESIGN... YES I SAID FOLDABLE🤘🤣 , making it compact and easy to install, it's IP65 WATERPROOF and will give you no worries while growing in a humid environment and makes it a flexible high performance commercial grow light for enormous  SUPER FROSTY yields.😛😛😛. MARS GETS EM FROSTY BOYS👍 AWESOME HEAT DISSIPATION: The Low energy consumption FC4800 growing light has 6 passive-cooled bars and an aluminum heat-sink a removable driver  you can upgrade later or have it outside your tent. with zero noise  you can save up to 50% in your electricity bill compared to HID's or HPS lights! It also has a removable driver so you can upgrade it in the future or have it outside your grow space for better heat control in your growroom👍 UPGRADED FULL SPECTRUM: Yield Max Spectrum with enhanced red (Top-Bin Samsung 3000k-5000knm, Osram 660nm Deep Red), speed up blooming & fruiting, perfectly suited for full-cycle growing. The FC4800 LED grow lamp was born for Big Buds, perfect for commercial, grow tent, grow room, hydroponics, and all soil grows.👍 THE BEST SAMSUNG DIODES: THE FC4800 has Top Bin genuine Samsung Osram Diodes, combined with remarkable 2.8 µmol/J Efficacy, 30% higher Average PPFD, 50% less energy consumption, to maximize your yields. They give you a TRUSTED 3 years warranty 100% Satisfactory guaranteed!!! 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Over 90% light energy can be absorbed by plants, it will have higher intensity in a MARS HYDRO grow tent with diamond design mylar, or by crossing over using multiple TSW2000 HIGH EFFICIENCY FULL SPECTRUM INDOOR GROW: 3000K 5000K and IR 660nm 760nm RED, makes it close to natural light, highly engineered to provide improved growing performance for plants at all stages of growing, rapid plant response from seed to flower; Uniform and wide-beam light distribution for your plant's optimal growth 8x8 1680D GROW TENT The MARS HYDRO 8x8 Grow Tent interior is made of a unique diamond design mylar which is highly reflective. (Other companies mylar and reflective rate is as low as 50%, and it won't provide efficient light. They use thick 1680D oxford canvas, and being double stitched, it is tear proof and perfect for locking in the light. Their grow tent keeps 100% of the light inside the tent and helps your plants absorb more light. EXTRA-THICK material means No light leaks or rips at all.👍 Strong Stable metal corner adapters and poles are the trustful supporters of the tent. It's very sturdy, neither rusting or paint flaking off. quick and easy tool-free setup. They use heavy-duty metal zippers, with double layer black lining, by far the best on the market which creates a light-proof seal. The Easy observation windows are made by double layers of cloth. Observe the growing conditions of your plants without opening the tent and avoid disturbing your plants. The observation windows also serves to dissipate heat and ventilate the air. The tent includes 2 removable waterproof floor trays to hold soil and water. The removable tray can be taken out to wash easily. They guarantee the grow tents are easy to install even if you've never done something like this before. Clear Instructions and no tools needed! They also offer 30-day unconditional refunds for quality problems. Just contact their friendly 24 hours customer service 👍
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@MeaCulpa
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8th week of flowering. There is a light at the end of the tunnel. After a tip from here, I put all the LED lamps I had in the tent. Doesn't matter how cheap they are. The two light bulbs produce 6500k daylight... And it really bears fruit. The resin production around it is clearly visible. I'm excited. I realize that the trichome photos are a little too early, but I'll test the photo quality.
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I had to harvest both sbc.. they produced seeds caused by too high EC in the Coco. The EC of the GP was even higher but she seems to handle it better and performs normal. A EC of 1.8 in the Coco was too high for the sbc. EC of the GP was 2.4. I flushed the GP, it was now possible since the sbc wasnt there anymore and therefor was no danger of getting the stacked pizza boxes wet which would let the sbc sink down like in mud. GP will be harvested in two weeks and right after that i will start a new diary with 3x sbc. I then can flush probably and will not run into this problem again.
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@yvet_te
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Finally have a day off and got the babies into 3 gallons and into the tent today! I used Fox Farm Ocean Floor to repot. Running behind schedule in veg, but ready to see them take off. Also taking them off 24/0 light schedule and putting them on 18/6. Last grow I had a spider mite AND a mildew problem (sad face) so I sanitized the heck out of the tent and got a new oscillating fan. You can see it blowing the plants around in the video, making them all strong and shit lol.
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Pretty happy with the grow and the harvest, the drying went well also! Each grow highlights changes for the next and this has been no different! I'll be making more training adjustments in the next runs to try and refine the vertical scrog and get to the point where it's a wall 1 bud thick! I know it will get there in the end it's just like relearning how to do a horizontal scrog! I just need to predict the way the plant will respond when the light is from the sides! Bravery and brutality during pruning I think is the key! 😂✌️💚
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@Roberts
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Baked bomb is doing great. She got a few spots on the leaves from getting wet. It is not anything I am worried about. She will only get so big in this potter. I imagine she will still turn out nice size if I keep up on her. She got her first lst and selective defoliation. She has been getting fed about every other day with ph water put on to keep wet between feeding. She is good to go. Thank you Bomb seeds. 💣🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g
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Week 9 for Moby dick by fastbuds, She was fed once last week at the start of the week with regulator by Aptus & FPJ too. Then the stress of the week it rained so she was just fed from that i guess😂 I did a tempt to place her indoors when it was raining heavy before night time but didnt catch her every time. I think shes going to be a long living auto😂 Because theres no way she will be done in the next 3weeks. Lets see if she proves me wrong
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2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.
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Day 141. Girl is thriving, but now nobody will close greenhouse for nights and watering will be very limited... In two weeks I am back and will give her all TLC she will want. She is the healthiest plant in all greenhouse ;) Happy Growing !!!
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Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 36 since time change to 12/12 h. Hi guys :-) The buds develop quite well on the mini plant :-). I gave her another 1 g of GHSC Bio Bloom during the last pouring added per l coco and 1 g enhancer. That is enough defensively until the end because I would rather fertilize the little lady less, even if she shows deficiencies. I am curious to see how the experiment will progress and I will keep you up to date :-) Stay healthy 🙏🏻 You can buy this Strain at : https://www.zamnesia.com/de/5165-zamnesia-seeds-kalini-asia-feminisiert.html Type: Kalini Asia ☝️🏼 Genetics: Black Domina x Purple Kush 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .