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Blüten werden inzwischen sehr Harzig und der Duft nimmt zu. Dem Giesswasser wird täglich 1:10 Jauche zugegen. Zu dem jedes zweite Gießen Pro Sugar von Grow Pro ein Kohlenhydrat Blütedünger(von der Mary Jane ein Tester). Zu dem gibt es ein bis zweimal in der Woche Seafeed Xtra von envii. Die ersten Blätter haben sich unten entfernt daher gebe ich gerade wieder Stickstoff zu den Pflanzen. Auch wenn es nur noch diese Woche war danach nur noch Blütedünger. Zu dem habe ich Basilikum gepflanzt, Dill, Ringelblume und Koriander gesäat am Anfang der Woche und zum Ende noch Lupine und Rot Klee.
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I had alot of Fun with this Grow, and a lot of Growers, had a Story about the Strawaberry Cough. Thank you all for sharing that with me!!! I learned alot about the heritage of this Strain and its appearing in Movies and Music. Mr. Kushman itself had a great Story about Strawberry Cough, and how he got them as a Present But back to the Grow fast Germinating, Germiating Rate 100 Percent, fast grower, and then the Journey began...... One Pheno turned out with Cottoncandylike Buds , The other Pheno with Fistsized Dense Buds Booth showed a wonderful Scent, and they were a Pleasure to look One Plant is still growing Thank you @Miister_lee , for the opportunity to grow them And LEGALIZE WORLDWIDE
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@Aedaone
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The temperatures, humidity, height, and watering volume(if measured) in grow conditions are all averaged for the week. The pH is soil pH. Any watering done by me is well water which is 7.6 pH and 50° F. Any listed nutrients are ml/gallon of soil spread evenly across the top of the soil. Day 1 we had a high temperature of 79° with mostly cloudy skies. The nights have been dripping below 69°F, creating an ideal environment for powdery mildew. I watered about 3 gallons of well water. I treated with Arber biofungicide. Lady bugs were released and are having an aphid feast. Day 2 we had early morning rain followed by mostly cloudy skies. High temperature was 77. The rain took care of the watering today. I'm treating with arber again and more defoliation. Day 3 we had a high temperature of 81°F, skies were partly cloudy, and I watered 3-4 gallons from the hose. Day 4 we had a high temperature of 83° with sunny skies. I watered 4-5 gallons from the well. I removed leaves with powdery mildew and treated with Arber. Day 5 we had a high temperature of 88°F. Skies were clear and sunny. The humidity was around 90% today. I watered 4-5 gallons from the well. I treated with Arber again today. Day 6 we had high temperature of 89°F. Skies were sunny. I watered about 4-5 gallons with well water. I've been treating these with Arber biofungicide and it's had some good effect. I stripped all the infected leaves.I'll get the third tomorrow. I'll treat these tomorrow with growers ally fungicide and see how that does. Day 7 we had a high temperature of 86°F with partly cloudy skies. We're expecting rain tonight and it's super humid today. The weather is not cooperating while I'm treating for powdery mildew. This week was a success. The plants gained on average 3 inches in height. They've slowed down their upward growth and are transitioning into flower. The weather has been atrocious for cannabis. These girls are super healthy but the humidity and cool nights have caused perfect conditions for powdery mildew. I began treatment with Arber biofungicide and it's working pretty good. I'm switching now and will treat late day 7 with Growers Ally fungicide. I removed the infected leaves and defoliated to help light penetration.
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@Viridios
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Hi all.. Lady's are very happy..still stretching a bit..looks like the push is almost over. Very faint sweet smell starting to come form them. Im going to do another light defoliation soon. Humidity is a bit high and i want to avoid WPM. Sorry for all the wierd couler photos , i try take picks as the lights come on.. I will upload some more picks on friday. Other then that..Perfect! 😁 420 for life.
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She grow big and i decided to let her flower. Lets see what the next weeks bring. Have a nice week
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week intel: its time to harvest some of plants the ones that is mature enough. indica dominant plants will done first always so we should harvest them first because my base nutrients and one of boosters was salt based, i'll do flushing this week to get some relieve to plants in the last days stresses : flushing Drought stress via watering only one time with flushing this week feeding: day 1 : i flushed them with Advanced Nutrients Flawless finish and adjusted ph day 3 : no more feeding from now on day 5 : no more feeding from now on guide of the week : i harvest in 2 parts : first i harvest top of the branches and will let the lower buds to ripe another week then ill harvest the second wave. indica dominant plants will get done 1 or 2 weeks sooner than sativa dominant plants that will often takes more than 8 weeks so be aware to harvest them sooner. my dry and cure style is this: 3 days of hanging upside down to get water activity lower to around 0.6 in 50% humidity and 26 C temp (i know its a little high but we are in a hot summer right now and i cant get it lower even with air conditioner) and then after 3 days of drying i remove leaves and stalks, trim buds and move them to jar for the rest of their life :D . and in the first 4 days of curing i open the jar door and let hem get some fresh air in the jar for about 5 minutes and close the jar door again, after 4 days of curing like that buds are smokable but they will get better as they getting cured about 1 month. im happy as hell with this harvest :D.
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@BiggBloom
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Dropped these babies in water on Friday around 10AM. By Sunday, 4 out of 5 seeds had little taproots, so all 5 went into solo cups half filled with pre-washed Canna Coco. I buffered that coco with gypsum + dolomite lime prior to using it and mixed 2 tsp of Gaia Green AP to the gallon of it that I used on these seedlings.
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@Chubbs
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Wow, another week done. It's become an absolute jungle in my greenhouse. A few of the GG4/Sherbets tops had to be mainlined since they're hitting the top of the greenhouse at 7ft8in tall. The smell is definitely getting stronger every day and it goes from super pungent, to sweet, to almost tropical. They're all still looking nice and healthy. The Athena Blended Line works wonders with my well water as the plants couldn't be happier. All in all Happy Growing.
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@Hashy
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Most of this week I was away so the tent was on autopilot. When i got back i was pleased to see all 4 strains where still around the same on development. The strawberry pie is the smaller of the 2 fastbuds autos. She is starting to throw out some nice colours now. I'm confident this one is going to hit my 13 weeks scheduled for it, so not long left. Notes Day 64 nothing away Day 65 autofeed last weeks nutes Day 66 Got back, everything's fine Day 67 manually water Day 68 light strength from 75% to 87% PPFD was 527 Dli was 34.2 now its 716Ppfd 46.4Dli. Day 69 autofeed this weeks nutes Day 70 653Ppfd 42.3Dli Back in a week Take it easy.
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@Naujas
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She is much prettier than she was a week ago :) I remembered that I have my first grow light, which is more compact and it will give me more space, so I changed my light, now the girl's side branches get light too:) I add a lot of video memes, because I really want to win Iphone16 pro ;) and those who don't take risks don't drink champagne:) good luck to everyone.
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@JBalz
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Pros: Well bouyz after 2 weeks of beasties and following a good flush the nugz are stacking up nice and dense. Blue dream have awesome, thick towering branches and no doubt will lead to some monster buds. White widow had some good growth but slower than the others but the trichrome production tops the others. The center gold leaf is still taking the lead on size and will most likely produce the most. Cons: the behemoth gold leaf in the corner would not slow down and is now, I believe, rootbound and possibly suffering from some rot. Select leaves in the center are turning yellow with brown spots and falling off. It doesn't seem to be affecting flowering but I took precaution by giving it a shot of root doctor. The white widow in the center is showing similar signs but at a way lesser extent. Could just be me overreacting but a few of the leaves are yellowed and wilted with small black spots (usually a sign of root problems). I usually leave some foliage randomly hanging at the lower section of the plants to remedy any problems that may arise before it gets out of control. Hopefully these issues don't progress and I can reap the rewards of a bountiful harvest. 4-5 weeks remaining.
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Transplanted Into Micro PC Grow Room On 1/21/19 At 10 am. Experienced Mild Stress As The Leafs Indicated Slight Wilting. 2 Hours After Settling Into Her New Spot. Observed Stress Relief. As The Leaf's Indicated By Stretching Upward For More Light Intake. Will Be Adding Growstones Tomorrow. After A Full Days Rest. All In All Not A Bad Transition Overall! As She Seems To Like Her New Spot. Air Temp Maintaining At A Comfortable 70 Degrees F. With Water At 60 And A Ph At 6.3! Day 9 Second Nodes And Fan Leafs. Roots Are Exploding! Got Here Hooked like A Crack Head On Emerald Goddess Green Crack. Splash'd Her With R.O. Water! Progress Is Stable And Looking Good! Tomorrow Jan 29 begins week 3 and top'n along with low stress training. Will be customizing a metal net file basket into a scog system for the begining of week 4 as I will be training her for vigorous sideway grow to maximize yield. I am just hoping I have the time as i think it will be flowering sometime in the late 4th to middle of the 5th week! Will update with week 3 photos soon!
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@GrowGuy97
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First auto run! Fastbuds , Seedsman & Victory seeds! Never done autos before so excited to see how this goes! Day 5 and 2 of the NHL’s from Victory seeds still hasn’t popped😫😬
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I had so much fun learning how to grow, and how many different ways there are to grow! I love the community here!
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Continued growth on day 105, as October gets closer the days get shorter and the nights a bit chillier. The plants have continued to develop beautiful trichomes and the smell is scrumptious. I still find a few caterpillars here and there though i have prevented most of them from destroying the plant. Please do not neglect to check up on the state of each bud if growing outdoors.
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.