WEEK 2 DAY 14 Since last week the babies have taken off! They didn’t have much light until yesterday when I added another 100w led and since then I’ve seen a huge difference ! They are looking super healthy the roots said fuck the pot and grew right through it I’m actually super interested in seeing how these grow up.. The 3 clones that were separate last week are now moved into one single pot to further develop the experiment. They are on the same feed as the mother with flora grow/micro/bloom and cal-mag . And In the last picture you can see my ghettos man way of cloning .. going to see which 2 out of 4 are better rooted and I will move those to a pot in about a week or so. If you guys have any questions or want to know where I got these exotic seeds message me!! Or comment below !

No rain from April, it rains the day before the harvest........WTF Perfectly ripened, this half of a plant will become among the prettiest buds ever grown. See U in few days for harvest. Spoiler: great smoke!

Just another update for the King's Juice by Green House Seeds, I have uploaded a video for you guys which gives you guys a better look and how I'm progressing and how I'm training the ladies. Any questions guys just ask. Happy Growing 👍🏾🌱💚😎

OG 4Q24 Seedling Bolt III Orangegasm (Fem) [ IRIE Genetics ] 12/12 @ Bolt (Day 21) Germination: 20 November 2024 #3A Earliest Harvest Date: 9 February 2025 #3B Latest Harvest Date: 19 February 2025 _________________________________________ Start of Week: [Wed Dec 25, 2024 CR2 4Q24 1:B:1:1] End of Week: [Tue Dec 31 , 2024 CR2 4Q24 7:B:1:7] OrangeGasm Fertigation: Max EC: [ 2.4, mS] _______________________________________ Week’s Objectives: - IFF We’re still HAND WATERING, ADD First Resin Bloom to Full @ 4ml/gal. - We can’t replace RLA’s Carbon Component with/out ~300 ml/Gal Ful-Power (Fulvic Acids) We WON’T be doing that. Rather, we’ll see a diminution of Carbon provided, look for 3-5 days to stabilize after switch to auto-irrigation. EC Starting Water: ec: 0.0 mS/cm, pH: ~7  __ Wed Dec 25, 2024 # Christmas Day OG 4Q24 15:B:2:1 Watered late tonight (~2200). - NO RUNOFF - Barely the beginning of Burnt Tips - Grew 2” overnight - raised light 2”, Canopy Distance is 19.5 @ 77% (770 ppfd) After Watering - With REDUCED Light, Plants are FRISKY Leaves raised (but no fingers in light sockets) - # We’ve got Pistils (Noted Yesterday) - Ceasing Foliar . . . IT’s been good. ;-} Runoff: NO RUNOFF Fertigation: - [x] Primer A&B: [ 10.2, ml] - [x] CalMag Fuel: [ 6.8, ml] - [x] Silica Skin: [ 6.8, ml] - [x] Lush Green: [ 5.1, ml] - [x] Root Anchor: [ 5.1, ml] - [x] Peak Bloom: [ 5.1, ml] - [x] Photosynthesis Plus: [ 6, ml] - [x] Quillaja 60 Powder: [~scant~] - [x] SLF-100: [ 5, ml]  Standardizing Lighting __ Thu Dec 26, 2024 # BOXING DAY OG 4Q24 16:B:2:2 Scotoperiod: [ 12, h] EC: [ 2.4, mS/cm] Temp: [ 79, °F] RH: [ 68, %] VPD: [ 0.85, kPa] NOTE: If we’ve no runoff today, Water AGAIN at Midnight, Install Auto-Fertigation Tomorrow Raised Light 6 Clicks - Grew 2” Overnight - Light Distance ~20” @1900 - Fertigation Fertigation: - [x] Primer A&B: [ 10.2, ml] - [x] CalMag Fuel: [ 6.8, ml] - [x] Silica Skin: [ 6.8, ml] - [x] Lush Green: [ 5.1, ml] - [x] Root Anchor: [ 5.1, ml] - [x] Peak Bloom: [ 5.1, ml] - [x] Photosynthesis Plus: [ 6, ml] - [x] Quillaja 60 Powder: [~scant~] - [x] SLF-100: [ 5, ml] IPM: - [x] Zymes Bomb - Top to Bottom, including Airpot Sides.
Foliar DRENCH w/ Zymes at 1:31 water dilution. Drenched top and bottom of leaves, stems, JOINTS, Coco Top, and spraying down ALL Airpot Side Holes, and BOTTOM OF AIRPOT. Letting the girls run up to 82°F, RH @ 69%, VPD ~9. Plants are HAPPY w/ EC: 2.4, w/ Moderate High Lighting (~770) If they’re happy before lights out, and I know it - Let’s raise light to 80% (800 µMol/m2/s), adjust airflow for overnight Temp (Humidity Down). And call it a night. After Raising light to 83%, REDUCED again to 77% @0600 __ Fri Dec 27, 2024 OG 4Q24 17:B:2:3 Runoff: [ 500, ml], EC: [ 1.7, mS/cm] Raised Light 6 Clicks (Grew 1.5” Last Night). Light Distance back to ~20” 1.5 Gal fertigation - let’s do 1900 & 1200  Fertigation: 1.5 Gal # 2000 & 0100, .75 gal Each, EC: 2.5 - [x] Primer A&B: [ 14.8, ml] - [x] CalMag Fuel: [ 9.9, ml] - [x] Silica Skin: [ 9.9, ml] - [x] Lush Green: [ 7.4, ml] - [x] Root Anchor: [ 7.4, ml] - [x] Peak Bloom: [ 7.4, ml] - [x] Resin Bloom: [ 4.9, ml] - [x] Photosynthesis Plus: [ 9, ml] - [x] Quillaja 60 Powder: [ 0.125, tsp] - [x] SLF-100: [ 7.4, ml] After 2:30 Feeding - Raised light to 83% __ Sat Dec 28, 2024 OG 4Q24 18:B:2:4 Temp: [ 82, °F] RH: [ 68, %] VPD: [ 0.96, kPa] Light Distance: [ 19.5, “] Fertigation: RLA Heavy, Flower Week 3; EC: 2.4 Notes: - Evaluate 83% Dimmer at Lights On - Back off to 77% if any indication of Light Stress - Let’s ramp to EC: 2.7 mS As max. Beyond here, we probably don’t have sufficient CO2. Light Intensity (IDEAL): 83% # ~830 µMol/m2/s EC: 2.4 422 ml/plant, runoff, EC 1.5. Fertigation: 1.5 Gal # 2000 & 0100, .75 gal Each, EC: 2.5 - [x] Primer A&B: [ 14.8, ml] - [x] CalMag Fuel: [ 9.9, ml] - [x] Silica Skin: [ 9.9, ml] - [x] Lush Green: [ 7.4, ml] - [x] Root Anchor: [ 7.4, ml] - [x] Peak Bloom: [ 7.4, ml] - [x] Resin Bloom: [ 4.9, ml] - [x] Photosynthesis Plus: [ 9, ml] - [x] Quillaja 60 Powder: [ 0.125, tsp] - [x] SLF-100: [ 7.4, ml] 420 ml/plant op Crinkly LOWERS - EC Too High Too Fast? __ Sun Dec 29, 2024 OG 4Q24 19:B:2:5 EC: 2.3-2.4 The Max Hydro comes in at 2.36 EC. RLA Hydro Chart set 3.4. How Can Be? DROPPING SILICA SKIN from RLA Hydro (Eliminate Brown Patina, and Small Orifice Build-up) RLA Hydro  Nutrient Element Ratios ppm mS/cm NER N 262 0.5 21.8% P205 143 0.3 11.9% K20 476 1.0 39.7% Ca 214 0.4 17.9% Mg 48 0.1 4.0% S 57 0.1 4.8% Si 0 0.0 0.0% C 333 TOTAL 1533 2.4 100.0%  
RLA - Full - [x] Complete Irrigation Install Defoliation I We want to eliminate All Vegetation from TOPPED NODE (3) Down. - [ ] Count NODES FROM SPLIT - [x] Measure Plant Heights: 11 - 17” - [x] Remove Each Pot and Trim OUTSIDE OF TENT (Get it RIGHT!) - [x] Can we adjust the pot positioning to get ONE UNDER CANOPY Fan? - [x] Eliminate FANS and Growth tips NODES 1 - 3 (3 is TOP SPLIT) - [x] Eliminate FAN LEAVES from First Node of MAIN STEMS - [x] INSTALL DRIP RINGS (No Manifold, Yet) - [x] Apply LABEL to Each Plant - [ ] Consider Eliminating FAN LEAVES from Second Node of Mains _______ Fertigation: 1.5 Gal # 2000 & 0100, .75 gal Each, EC: 2.5 - [x] Primer A&B: [ 54.1, ml] # EC: 2.4 MAX - [x] Photosynthesis Plus: [ 24, ml] - [x] Quillaja 60 Powder: [ 0.25, tsp] - [x] SLF-100: [ 15, ml] Time Set Initiate Complete Event 1 18:45 18:45:25 Event 2 20:05:00 20:05:25 Event 3 21:25:00 21:25:25 Event 4 22:45:00 22:45:25 Event 5 00:05:00 00:05:25 Event 6 01:25:00 01:25:25 Event 7 02:45:00 02:45:25 Event 8 04:05:00 04:05:25 Event 9 05:25:00 05:25:25 CHANGED TO 25 SEC EVENT (from 20) Tuesday - / / / __ Mon Dec 30, 2024 OG 4Q24 20:B:2:6 - [x] Harvest Dehu Water Continuing to RAISE LIGHT ~ 2”/day (6 Clicks of the support ratchets). Runoff: - [ 422, ml, plant!!!!] # Reduced Event duration to 20 Seconds (from 30). - [ 1.4, mS/cm] Lowering light to 70%, see if lower fans relax. They’re electric. :-). They relaxed. Inner fans surrounding apical stems are laying @ 30°, rather than Reaching for 60°. I have perhaps been overnighting. HUMIDITY IS CRANKING. It’s impossible to get VPD above ~8.3! Trying to keep humidity below 68 drives Temp below 78. I forgot about the part. ;-( Dehumidifier is ON, Doors are Closed, Room Vent is CLOSED. Dehumidifier is delivering 50%RH to the room. Orange Gasm is quickly filling the air with moisture. At MIDNIGHT, Raised light to 77% ( ~770 µMol/m2/s] pH Adjusted 2 Gallons of Filtered Tap to pH: 7.2, EC: 0.3 (Used for Dilution when refreshing reservoir with DEHU and Fresh Supplements. __ Tue Dec 31, 2024 OG 4Q24 21:B:2:7 @0630 Took a peek in the tent. SCANT RUNOFF @ 20 Sec Feed Events. JUST Touched TIPS (Burning). Bumping Fertigation Events to 25 Seconds, after Feeding 9 * 5sec (45 Sec) To bring DAYS delivery to 25 Seconds. Check Reservoir: - We’ve 4 - 5 Gallons. @LIGHTS ON - Reset Light Height - Reduce light to 73% (See if we can avoid more tip burning) Harvest Dehu: - [x] Amount: [ 5.7, l] - [x] Primer A&B: [ 27, ml] # For EC: 2.4 mS/cm, Nailed it, First Try. ;-} Orange Gasm likes to SPREAD when Topped. - Which makes her a funny candidate for SOG. ;-} 

Diese Woche habe ich meine Pflanze nur einmal gedüngt, ansonsten nur mit Wasser, CalMag, SuperVit und GreenSensation versorgt. Ich sehe immer noch leichte Anzeichen von Überdüngung. Allerdings entwickelt sich die Pflanze wunderschön und verströmt einen intensiven, fruchtigen Duft nach Orange und süßen Aromen. Zudem ist sie extrem klebrig, wie man auf den Bildern sehen kann. Auffällig ist auch, dass die Blätter dunkler geworden sind. Dieser Effekt ist für mich neu, da es meine erste Sorte ist, die so reagiert. Es ist wirklich faszinierend!😅🤤 This week I only fertilized my plant once, otherwise only supplied with water, CalMag, SuperVit and GreenSensation. I still see slight signs of overfertilization. However, the plant develops beautifully and exudes an intense, fruity scent of orange and sweet aromas. In addition, it is extremely sticky, as you can see in the pictures. It is also noticeable that the leaves have become darker. This effect is new to me because it is my first strain that reacts like this. It's really fascinating! 🍊💐🎂

Week 7 we can see the beauty behind exotic genetics creation. Strawberry candy terps present heavily at this point.

This week, we will transplant the plants into large 19-liter pots and begin training LST immediately. Once they have recovered from the stress, we will proceed with fimming."

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Here we are :) the are finished

Les filles ce sont bien adapté a leur nouvelle maison.

First week of flower👏 They got 2 hours extra darkness before flip. Not because of scientific evidence, but simply practical. Still looking back to the first weeks of veg. WHAT A MESS and WHAT A MIRACLE 🙏 Coming into flower, they had two defoliation and the latest one was 7/10. Last feed 8/10 Introducing Biobizz CalMag(0.5ml/l) + BioBloom(0.25ml/l)+Topmax(0.25ml/l)+Rootjuice(0.25ml/l) Still 2ml/L Fishmix+BioHeaven(Fishmix will be switched out with BioGrow) 👽👉10/10 VPD is 1.2,-1.3. good airflow above and below canopy. 👽👉11/10 Mild signs of light stress. They will get used to the VPD. Can't wait to feed in a couple of days and see the reaction. Im sure they are really hungry! 👽👉12/10 Waterday** Introducing higher doses of bloom nutes. The Run off PH and EC are all within the limits 👽👉13/10 They lit up after some nutes. They look happy! Let the stretch begin 👽👉14/10 Already see fast growth. Just monitering factors, to make sure the climate is optimal and their are no symptoms. 👽👉15/10 Waterday* Thirsty ladies 👽👉16/10 Last day of week 1 of flower. Everything is going fine, Good airflow, None of the small symptoms has gotten worse, so overall happy ladies! How ever one has a little stunned growth or maybe just a bit behind of going into stretch

17-05-2021: As you might have seen I have added some reflective foil to reduce heat on the water. This helps a lot. Growth is as expected but they are a bit on the bright side. Well yesterday the set EC was raised from 1.3 to 1.6 so probably new leaves will be a bit darker. Also added a bit of Calmag. Still great to see that as soon the roots touch the water the growth is exploding. Well yet 13 days till 12/12 so I am excited to see how big these ladies will be by then. Hoping for a seventh node before that. 18-05-2021: Today I dosed 18ml of H2O2 (50% silver stabilized). I have seen a few algae so if they are gone by tomorrow I keep this dose, if they are not I will give them a shock treatment of 50ml. In this system I don't have to worry about clogging (50mm tubes) but I also believe that if it is not necessary I shouldn't do it. Dosing is advised from the supplier based on tomatoes by the way. 19-05-2021: All survived the H2O2. Today I have added additional 18ml. Just to see what happens. Growth seems a bit faster last night 22-05-2021: Blue is not doing great... I hope they survive. Red is still OK

Going nice;))))

Semana 4 (5Nov-11/11) 7Nov - Regada com 2L d água

Día 15: 🔹 Primer riego con toda la gama de productos BioBizz, una EC ajustada a 900 y pH a 6.2. Le agrego Rootbastic para una mejora de las raíces. 🔹Sigo pulverizando las plantas con una EC de 400ml, con acti-vera y Rootbastic. Van de miedo, sin quemaduras ni signos de alguna carencia. 🔹Le incorporo al armario un higrostrato, que va conectado al extractor y al humificador que también incorporo para mejorar las condiciones del cultivo. Día 16: 🔹Pulverizo las plantas 2,3 veces al día con Rootbastic a 0,1ml/ y Acti-vera BioBizz a 1ml/l Día 17: 🔹Riego según la tabla de nutrientes con una EC de 1000ms y pulverizo las plantas cuando se despiertan con enraizante y una EC de 300 Día 18: Aplico foliarmente 2/3 veces al día una solución de Rootbastic a 0,1ml x litro. Día 19: 🔹Trasplante a maceta definitiva de 11 litros 🔹Aplico Special THC Plus foliarmente en base mínima establecida por el fabricante, ya que en unos 10 días aprox. la pasaré a floración. 🔹Riego con una solución suave de abonos EC de 900 y un pH de 6.2 Día 20: 🔹Aplico foliarmente 2 veces al día una solución de Rootbastic a 0,2ml x litro. 🔹Aplicación tierra de diatomeas en las nuevas macetas para evitar futuras plagas. Comentar que es la primera vez que la utilizo y ha sentado genial al cultivo. Espolvoreo una cucharada por cada maceta por encima del sustrato. Día 21: 🔹Descanso. Comentar que el trasplante las ha sentado genial.

It's been a while but the ladies are doing great. Some pretty nice budformation. 1 of the ladies has some special looking buds, I wonder how they'll finish.

Plants are looking ridiculously dank rn. Buds r coming in nicely. Lights on 11.5 hours on and 12.5 off I believe that will speed up flowering. I also gave it 36 hour lights off period b4 I started flower. As u know I gave it a top dressing first day of flower. Well it’s been 3 weeks so I gave them a feeding of coconut water. And not the cheap stuff either. Next week I’ll give it organic fish emulsion, then molasses, and last will be a half molasses half honey mixture. I’ve had no issues mixing sugars as I believe different sugar sources have different benefits. Based on what I read about each of them. Molasses is supposed to be the best. Everyone says that. But I believe coconut water will battle that. Who knows maybe when I grow outdoors I’ll do an experiment and test which sugar will work best. I’ll even test a couple plants with mixed sugar which my hypothesis is that that one will work best.

Week 11. Day 35 roughly, most of these are 63-70