Guten Morgen liebe Growfreunde 💗 Und herzlich willkommen zu woche 16🤗 Es geht langsam dem Ende zu und ich bin so nervös und gespannt😅😅 Zum Verlauf kann ich nur sagen alles läuft supi, wieder keine Probleme gehabt in dieser Woche. Das ist sicher mein entspanntester Grow. Bin mit dem Autopot One Pot 9mm System mit den 15 Liter Töpfen sehr zufrieden. Lass das Wasser nicht dauerhaft an, entscheide nach Gewicht der Töpfe. Wenn ein Topf noch zu schwer (nass/feucht) ist, dann wird er im Untertopf unterlegt, damit er kein Wasser sagen kann 😉😎 so lässt sich das ganz gut steuern. Ich denke wenn man nur 1 oder 2 ähnliche Genetiken hat dann müsste das System noch besser arbeiten. Aber wie gesagt, kann es nur empfehlen, da es eine Menge Zeit und Streß spart, für euch und eure Pflanzen. Wie immer wäre es toll, wenn ihr kräftig kommentiert, liked und folgt. In ein paar Tagen geht die Ernte los. Es wird 6 Harvests geben. Jede Sorte bekommt eine eigene Ernte Dokumentation. Kommt auch gerne in meine Facebook Gruppe: "Cannabisanbau für Anfänger und Profis". Ihr seid alle herzlich eingeladen liebe Growfreunde 💗 Also dann, bis zum nächsten mal👋 habt euch lieb und tut gutes liebe Growfreunde 💞💞💞 schlechtes gibt es da draußen genug🤔

Just a beautiful east growing plant. Added supplemental lighting due to the large canopies. I take a very hands off approach, let the gentics and the plant do it their way. Looks like it will live up to its name, so many buds on this girl.

The plant has a few retarded leafs for whatever reason, but it's healthy in color and still growing. Nothing to report about though.

These one was a big messy jorney, everything happened from almost kinling them, to almost no space for them to grow, LED broken middle flower and still perform like the queen champion she is!!! I am felling her mature over cycles , i am thinking of her like a wine vine that gets better over time , these one in my humble opinion its a must in everyones garden <3 <3 <3 Wen harvesting these one i felt the need to break it all apart to prevent bud raw or anything we do not want on our medicine, i like to hang them all and let them dry but in these case there was no chance i was taking that big of a risk with these big fruits all over the place, what a blessed run, my rack is completely full, there was no more space , i mean no more space at all hahah amazing , thank you thank you thank you <3 <3 <3 As always thank you guys for your love , your time, your support and it all, i fell blessed and i am truly thankful <3 <3 <3 All info and full product details can be find in can find @

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#aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #zamnesiaseeds #growerslove

With true love comes happiness <3 <3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 

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GooD Luky everiyvan💚🌱🌱🔝

First week back with my plants and they’re wayyy bigger than I thought they’d get! Decided to do a big defoliation since it’s about the 3-4 week in flower. And they’re getting stanky

Week 13 harvest! The girls are getting 12 hours of darkness before the hang! I can’t control my humidity yet when it’s completely dark otherwise I would’ve gone for a bit longer. My stinkiest frostiest prettiest plants yet…very excited to jar them up!!! And i’ll clean my fan soon too hahaha

I deliberately sent my 2 ladies into flower 2 months earlier. roughly the 12/12h rule. Sometimes it was 10 hours in the sun, but sometimes 12 hours. In my region it will soon be rainy and cold. I'm toying with the idea of harvesting them before I catch mold or something else. The trichomes are all milky. About 15-20% amber colored.

Day 100 - (yes I know it's late) She is getting near the end now. Loosing a few leaves. Trichomes milky but not amber yet. Pistils still white in places. I added a 500ml Monster can for scale 😂 hope it's allowed............ 😋

14 dias floração

Welcome to Bud Boutique Grow Diary - really appreciate all your love and support :) Dont forget to check out my other current grows! 🗓️ THE END: - After 14 Day of drying with around 15-16°C and 60% rh its finally time - LETS TRIM - beautiful woody kush smell while trimming - bud full of Trichs all over (shinning like diamonds) - 1/3 Phenos Ive didn't catched up that good with calmag had pushed out their Sugar out of the Buds. Really beautiful to see - never saw this before - really rare to see! - Curring them in Grove TerpLoc Bags - Watch out for my upcoming smoke review! Thank you for staying with me - This wonderful Journey finally come to an end. Next run is waiting already 💚 ___________________________________________ --- 🌱 Strain --- 🏷️ OG KUSH by Barney's Farm https://www.barneysfarm.com/og-kush-646 ---💡 Lighting --- 💡LUMATEK ZEUS PRO 600 https://lumatek-lighting.com/zeus-600w-pro-29/ --- 🥗 Nutrients and Feeding * 🍸 PLAGRON Algae Baseline grow/bloom + Additives: Power Roots, Sugar Royal, Pure Enzym, Silic Rock, Power Buds, Green Sensation * 📅💪 baseline grow/bloow: 4ml/l & additives: 1ml/l each https://plagron.com/de/hobby/produkte --- 🏭 Grow Setup --- * 🏠🌿 Indoor: Homebox 120x120x200cm (4x4) * 📐🌀 PrimaKlima exhausting Fan 1180m3/h (running on 60-80%) & Can Light Filter 800m3/h & 1x Fanbox 1x Dyson fan for Air circulation https://primaklima.com/de/shop/ventilatoren-de/ec-ventilatoren/pk160ec-tc/ https://canfilters.com/products/filters/ All Likes and comments are highly appreciated!!! don't forget to check out my Instagram: budboutiquee - Bud Boutiqe

guys this plant is monster!!! that is all i can say!!! cant wait to taste her one bad news, i have to take her out of the box for about 2 weeks before my Mephisto testers finished stretching, i have very small box ((( soon will move to the tent in house ✊👌

finally it reached the size needed to start training. topping done on the 5 knot and lst in the form of a star.

I loved growing this plant. I have learnt so many new things. This is the first time I topped, defoliated, attempted to mainline, and everything seems to have worked out pretty well. I have made some mistakes here and there, but that's part of the learning process and lead to a pretty interesting grow :).

It's flowering time or breeding time lol...Welcome the pre flowering stage I can't wait to cross her with the SKUNK #1 pollen. So far she I looking good.

2017-09-11. Kl 12.00. Week 4 starts. I have cleaned the whole room for the new week and gave the girls water and nutes. Added videos and pics. Girl is 21 cm high. ---------------------------------------------------------------------------------------------------- 2017-09-12. Kl 10.00. New pics and video. She has grown from 21 cm to 25 cm in 23 hours. ------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-09-15. KL 10.00. New pics and video. The girl is 30 cm high and has grown 9 cm the last 4 days. ---------------------------------------------------------------------------------------------------------------------------------------------------- 2017-09-16. Kl 10.00. She is growing like crazy and needs to get defoliated every morning. I added 2 new videos and gave her 2 liters of water and nutes. ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.