Day 37: Watered each plant with 0.8L with nuts 1516 ppm, 3221 us/cm, 3.2 EC Still giving sensi grow to the gorilla cookies Day 40: Watered each plant with 0.8L with nuts 1594 ppm, 3391 us/cm, 3.3 EC Still giving sensi grow to the gorilla cookies Did some defoliation

The plants are all doing well, unfortunately they are developed differently, in the future I will make a report per plant

Black Opium is looking gorgeous at this point she has been moving into flowering stage brilliantly, I can see flowers forming and emerging! Can’t wait to see her buds!

10/16 So the girls are doing well. They have been watered twice now and #1 just had what looked like a tiny bit of nute burn but took much better to the second watering. They are absolutely exploding veg wise and raising the light was necessary as #1 was much to short, will be updating with transplant soon! 10/18 Ok so transplant happened today. They were growing so quick we needed to move them as soon as they were water ready! They got transplanted into 5g fabric style pots. Did 2/3 Fox Farms "ocean forest" and 1/3 perlite mix. Used same nute mix for watering on the transplant, will change that up soon. See pics and video for more info! 10/22 There seems to be a nitrogen lockout. I'm guessing it was the unneeded cal mag add. Learned our lesson! Upped our grow big and giving the first water in big pots. Other than that they are growing amazing!

Tapering nutes off for the flush. Also added a small amount of Barley as a top dress to help her finish off.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

They been eating a drink very fast, I’m refilling auto pots every 3-5 days

Dopo 6 settimane di vegetativa per le nostre piccole talee, ormai velle grandi, arriva il momento in andare in fioritura e continuare a crescere e fiorire!!. Seguiranno aggiornamenti. Martedì 20/02/2024 Inizio controllo 2027 ec 5.5 ph Cambiamo soluzione e iniziamo con i nuovo dosaggi da prefioritura Aggiungiamo 75 lt demineralizzata Base 285 ec 7 ph Aggiungiamo i nutrienti per 80 lt 160 grow 160 micro 120 bloom 90 sensizym 90 calmag 90 rhino skin 90 bud candy Otteniamo 2146 ec 6.3 ph Aumentino al 100% la nostra mars hydro fc6500 e la teniamo a 40 cm di distanza.

Another amazing week with these beauties I am still feeding the same monkey nutrients couple seem to be burning through the feed quicker so I have upped the doses back to the full doses as I had dropped them slightly previously but they are so sticky 🙏🙏 so I am trying to ensure the air circulation stays constant and the humidity stays at around 42 with the increased temperatures we have had here in the UK I have turned my extraction fan up to near jet engine status 😂😂 thankfully its not really any louder than a fan so it's not been to bad and the carbon filter is doing a really good job at keeping the danky smell in the tent personally I love the smell not sure the neighbours would so much 🤣😳 in inlet fan and circulation fans are working hard but managing to keep temps from going above 29 there was one day I set Zues pro at 75% just to help temps drop The buds are really starting to get heavy now and there is literally no room left for anymore the highest tips are probably 4f 8 the tent is only 6ft 6 so the Zues pro 600 is as high as it can go now so thank goodness the home stretch is not that far away now. So I am really talking to them lots giving them lots of love and attention and preying these beauties continue to do there thing and grow there buds nice and fat with no issues 🙏👌 Thank you for reading stay blessed 💚

Week 19 (21-10 to 27-10) 21-10 Temps: 20.1 to 24.6 degrees Humidity: 51% to 61% Watering: Both 600 ml. 22-10 Temps: 17.2 to 25.2 degrees Humidity: 47% to 60% Watering: Both 1000 ml. 23-10 Temps: 19.8 to 24.8 degrees Humidity: 51% to 57% 24-10 Temps: 19.4 to 25.1 degrees Humidity: 59% to 50% Watering: Both 650 ml. 25-10 Temps: 19.7 to 24.6 degrees Humidity: 50% to 58% Watering: Both 500 ml. 26-10 Temps: 19.8 to 25.1 degrees Humidity: 50% to 59% Watering: Both 500 ml. Dry Weight #1: 3.4 kg. #2: 3.5 kg. 27-10 Temps: 17.4 to 24.9 degrees Humidity: 47% to 66% Watering: Both 500 ml. Dry Weight: Both 3.6 kg.

Last week before chop! I took a tiny test bud off and it smoked up real nice. you can tell by the buds that its going to be a rompa of a strain, thanks fastbuds grab your own at there site.🔥 2fast4buds.com I seemed to get the phosphorus problem under control, i made the mistake of not ph'ing my water after adding ph down.

Tried to FIM the plants not quite sure it worked. Decided to top them instead and see where it leads. Also added a net to support the stem from falling over.

To my haters: I love oxygen, so put a bag over your heads. Temps are dippin. They drink half gal a day! Hooo wee mofos so this week i got too high and over corrected ph up and down a medium bap cytokinin spray basically did what i want stunt height but also kinda burned it 😅 The trichomes did go bonkers so i dunno. Seems kush mints bottom right doesnt wanna move fast.

Smells amazing, buds are greesy to the touch…serious genetics from fast buds!

Finally into the last week of flower. It's been a long road, but the end is near and I couldn't be more excited. She has definitely bulked up over the last couple of weeks. The trichomes are milky with a few Amber's showing. I am busy flushing. I'm going to put her into 48 hours of darkness before I chop. Wish me luck.

Tag 42 - Ende der 6. Woche. Der Stretch ist beendet und die Pflanze konzentriert sich auf die Buds. Deshalb habe ich in dieser Woche den Dünger etwas erhöht und die Menge an Litern die ich gieße. Ich bin gespannt wie sie sich in der nächsten Woche entwickelt. An Tag 36 wurde die Pflanze gedüngt. Nährstofflösung: 2 ml/l - Voodoo Juice Advanced Nutrients. 4 ml/l - pH Perfect Sensi Bloom Part A 4 ml/l - pH Perfect Sensi Bloom Part B 2 ml/l - Bud Candy Advanced Nutrients. 2 ml/l - Big Bud Advanced Nutrients. Davon hat die Pflanze 1l an Tag 36 erhalten.

I topped and trained them two more times. I also lolipopped them, that is cut the bottom 2 sets of leaves and branches.