This week I decided it was time to employ some LST. Really I should have just done LST and not topped them, aside from the largest one which responded well. They weren't putting out fast enough growth to take to topping well, and I've learned my lesson. That's why my autos haven't done so well in the past. I'm still learning😬 So in order to get as much as I can out of them as we enter flowering, we're doing a little LST this week. Earlier in the week I did have to bring them in the hoop house on 7/11 because we got a lot of rain that day, our basement flooded and the water got so high in the stream that it washed the bridge out. So I think I made the right decision otherwise they may have gotten lockout. I also changed their feed a bit. I added in some Neptunes Harvest Fish Fertilzer 2-4-1 with the Alaska 5-1-1 in equal portions on the 13th and will continue with it tonight. I may add a little molasses as well. Most of what I was trying to accomplish with each plant is explained in the video. Enjoy!

Today I made a new portion of food with guano seaweed and palm ash. Plants develop well and are already in the middle of flowering.Everything is going according to plan and I look forward to harvesting.

As you can see, our macro lens arrived, we were able to take several photos and see the trichomes, we had never seen them so close before. We also noticed that many pistils have turned brown in the last days of this week but the trichomes are clear yet. The smell is getting stronger, reminds us of fresh fruits, mango and a little citrus. We are excited, she is healthy and smells good, but a little concerned with the development of buds and if they are within the standards for this week. Despite having many trichomes and the pistils turning brown, we are not noticing a significant fattening of the flowers (correct us if we are wrong). hahaha, noob stuff =)

Juste avant la récolte

Now planted in a 2 litre airpot.

Starting a new cicle. One of new money is 1 week younger. The Mid cicle is ready to flip The final cicle is near from harvest, maybe on 14th february. some crosses of a male Girl Scout cookies with : White widow, Industrial plant, White diesel and (White fire og x bubbakush )all made in 2014.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Another week has passed and this lady seems to have recovered from last week's sudden problems. I just kept feeding with clear water, I suspect low PH and my solution fixed it. I also raised the light a little more, this SP 3000 is a true beast of a light, and especially the Kush in the middle couldn't handle it that well. Thank you @MarsHydroLED I did some final defoliating, only bundled up leaves and leaves covering major bud sites. This Orange Hill Special is something else though, Im happy to grow this, she is pretty and sophisticated in all, almost majestic. The buds are speckled with orange hairs, the shape of each like a round ball of ice cream. She was pretty from seedling on and looking back, I would have loved some offspring of this pheno. She is a thrill and no signs of male parts on this one, although it can happen with this strain. So glad I have some more seeds and cant wait to put a tentful of this. Don't let its low score dissuade you, yes there is a chance she hermies but when she doesn't my my my... Check em out here @DutchPassion_Official She smells of orange and tangerine with a very strong hazy undertone. This combination promises a great smoke and frankly, I cant wait. Thanks for reading up on me and thank you for the comments and likes again! Hug Bud

Нам уже мало места, много новых стволов после lst, жду на днях новый гроубокс и лампу, так же через 2 дня начну вводить GHE nutriens

d33 Hoy subo fotos unos días antes de esta semana, mejor, tendremos más fotos. Hoy me he puesto a revisar un poco las plantas y me he dado cuenta de que las Lemon Krystal son hermafroditas, la que estaba en la parte de dentro, solo tiene unos pocos sacos de polen que todavía no están maduros, se los he arrancado y cortado 2 ramas que tenían mala pinta con eso, el resto de ramas parece estar bastante bien y son flores macho, la Lemon Krystal que esta mas cerca de la puerta del armario, esta bastante mal y la tendré que desechar ya que no quiero que polinice al resto de plantas. También una Kandy Kush, la de la derecha, le he quitado 2 flores macho, no parecía tener más, unas flores espontáneas, pero semillas a granel a veces pueden pasar estas cosas, no es lo normal, pero parece que la cepa de Lemon Krystal esta un poco jodida... Para añadir, la LK que parece que podremos sacar adelante(pero bajo supervisión) tiene una rama que tiene 4 cogollos en vez de 2, mola, pero si no se pone a sacar flores macho, la podremos continuar haciendo seguimiento. Tenemos un cogollo de la Kandy Kush(planta del centro) que es doble. Puede ser que tengamos algo polinizado en el Indoor, pero pues es algo que ha pasado, espero no tener muchas semillas mermara la calidad... d35 Unas fotitos rapidas... Parece que estan progresando las deficiencias, a final de semana añadiremos un poco m'as de abono a ver como responde

The girls are starting to wrap up their cycle. I have been watching the trichomes closely, and we are getting very close..Trying to pick the best day for both of the strains I have going at the moment, the Apple Betty are a little closer than the cookies, but they are very similar in readiness… I’ll be checking daily, I did get a wireless microscope for Christmas so it has become much easier to tell👨‍🔬 I’ve got mostly cloudy, with a few clear, but I am waiting for a bit more amber to give them the couch lock that I like when I smoke. Boxing Day was the end of week 8, so any day now! Happy Holidays Growmie’s! ❤️🇨🇦😎🎉

Finally found a balanced Nute mix 3-4 ml cal-mag 3-4 ml floranova bloom. The smell outside is wonderful where the vent exhausts. old leafs are being used up for energy, as the buds fatten looks normal for this plant. Whole this is top heavy now when it comes time to water the 3gal pot hardly keeps it upright. This is a 20% soil mostly coco mix so watering daily 1-2 times with 1.25gal.

Last week only watering one time and let the plant dry our for 3-4 days. There is not much to trim as the buds are rock solid and big.

Week 7 Apricot left 4 Strawberry Gorilla right 4

Recap.. Medium: Coco and Leca mix in 1L grow bags transpl to 5L bag Lighting: Greenhouse light by day, 600W LED by night Nutrients: EHG Micro & Grow, Biodyne, CalMag pellets, Phytha tonic, diluted fish tank water Training: Starting early LST Plants are now entering Week 5 of veg and responding well to the environment. The roots are well established in the 1L bags, and new growth is looking lush and vibrant. Started light LST to encourage lateral branching and even canopy development. They're drinking more now, so I’m adjusting watering frequency accordingly and alternating between fish tank water + Biodyne and a mild EHG Grow mix. Sprayed Neudosan as a preventative measure against spider mites picked up on Blue Cheese. No signs of pests or stress so far on the Supercheese clones. CalMag pellets are helping maintain strong leaf development and overall vigor. Planning to up-pot soon if growth continues at this rate. Everything’s on track – healthy, bushy, and ready to explode with growth in the coming weeks. 📸: Close-up of the canopy, LST in progress 🌱 Vibes: Green and clean

This week I just watered the plants with PH’d water and dechlorinated it as well. Going to give recharge when they dry up enough but I did notice the Purple Lemonade has gone a bit lighter than expected with its colors, like a light green leaf rather than dark healthy green. I’m thinking it could be a small deficiency of nitrogen since she did have a better stretch than the LSD’s, I’ll be topping her off with a small amount of 444 all purpose from Gaia greens lineup. Other than that the plants seem happy!