THIS WEEK THEY ALL BOLTED. I GAVE ONE GAL AWAY TO MY FRIEND. SHE'S INTERESTED IN GROWING. SO NOW IM DOWN TO 3 RHINOS. GROW IS VERY GOOD. I DECIDED TO LST 2 OF THE GALS. AND I'LL COMPARE THEM TO ONE W/O ANY TRAINING. SO FAR GROWTH IS VERY GOOD. VERY GREEN. AND STRONG STEM. NOTHING STRANGE.

I skipped a week cause it just keeps growing and swelling. A few days ago the branches started falling over. I couldn't find half the plant. So instead of ignoring it I decided to stake this plant up. Great smells, definitely seems like a winner. Other plants will have a hard time competing against this blue cheese. Earlier this week I let this plant dry out for a day to stress it a little. The yellow leaves are from too much light and the drought stress. I expected similar heights from the plants but as you can see the difference is huge.😊

The stretching phase has been progressing nicely - It’s a bit hard to see due to the low stress training, but growth is steady. The middle plant is a bit ahead in terms of development, though it should even out with the others over time. I’ve been dealing with a heatwave this past week, with temps in the grow environment peaking at 34°C. To combat this, I increased airflow with an additional fan and opened the tent flaps. Temperature is now back down to around 28°C, but the downside is that humidity dropped to 50–60%, which isn’t ideal for this early stage. Still, we push on! --- Quick rundown on everything else: Watering: 1500ml every third day Fertilizer: According to the BioBizz schedule Light intensity: ~1000 PPFD VPD: Around 1.54 kPa Temperature: ~28°C Relative humidity: ~55%

Welcome to Veg Week 4 of Zamnesia Kalini Asia I'm excited to share my grow journey with you from my Zamnesia Project . It's going to be an incredible ride, full of learning, growing, and connecting with fellow growers from all around the world! For this Project , I’ve chosen the Feminized Photo Strain Kalini Asia : Here’s what I’m working with: • 🌱 Tent: 120x60x80 • 🧑‍🌾 Breeder Company: Zamnesia • 💧 Humidity Range: 60 • ⏳ Flowering Time: 7-8weeks • Strain Info: 22%THC • 🌡️ Temperature: 26 • 🍵 Pot Size: 0.5l • Nutrient Brand: Narcos • ⚡ Lights : 600W x 2 I would truly appreciate every bit of feedback, help, questions, or discussions – and of course, your likes and interactions mean the world to me as I try to stand out in this exciting competition! Let’s grow together – and don’t forget to stop by again to see the latest updates! Happy growing! Stay lifted and stay curious! Peace & Buds!

Elle sera définitivement classée comme une strain dure à faire pousser. Une semaine elle a trop de nutriment, et puis cette semaine, elle semble en manquer. Les feuilles jaunissent jour après jour et je n'ai même pas flushé encore. Peut-être que c'est les foliar feeds avec le BooBloom?. Au moins, elle a de belle couleurs mauves et jaunes que je n'ai jamais eu avant, dommage que ce soit trop tôt. Je crois que je vais la flusher 1 semaine avant la récolte au lieu de 2 et donner un peu de nutriments à chaque arrosage pour les dernières semaines avec une plus forte concentration de Bloom. Je la ferai tester au laboratoire après la cure et j'espère au moins dépasser les 17%. Les top colas sont vraiment énormes et denses. https://youtu.be/ekomXrzOF3A?t=165

Explosion des nugs et des couleurs ! Une odeur de bonbon très forte ! Résineuse et collantes a souhait

Me costó adaptarme a la cantidad de agua que necesitaba, prácticamente 1.5 litros todos los días. Temperatura 23-25 centígrados, HR 45%. Me pueden contactar por ig @federicocabreraf , por si quieren saber mas detalles o algún comentario que me quieran hacer, paz.

*****Week 17 growth – March 27 to Apr 2, 2021 – Week 8 flower ****** We are in the first full week of flush now. Color change time and more swelling😃👍 GDP x GSC should have some purple in her......there certainly is in both. I have missed CL1 though, due to my actions. Pushing the nutes and lights is showing signs this week and both are fox tailing now. CL2 is holding it together though better than CL1........she said F you and shot her tips way up. Tails coming up the side of the bud as well, not excessive but they are prominent. They feel dense, yeah I squished a couple😏, so they should still have a good finish. CL2 is the looker right now and will have more dense buds I suspect but CL1 is showing that she can make some chunky LARGE buds if given what she wants.......without excess🙂 Frost levels on both girls is very high and looking sparkly😎 Aromas have been coming out more now and I pick up sweet and kind of floral......F I don’t know fully on these ones right now but it’s freaking good......smells like good weed!!! Running these both again I think. I was going to let CL1 go but color isn’t everything....CL1 should have fatter buds and I think she deserves another chance👍 CL2 is a given she will be run another time as well🔥❄️ 🔥❄️🔥❄️🔥 The girls are drinking well with being watered almost everyday. Moving into the phase of letting the pots dry right out before adding more water to ensure they are finishing all the nutrients in the media. Again, really just keeping them watered when they want it and taking pictures at this point😎 Little more detail….. Mar 27/21, Day 50 - 4L plain water at 6.0pH - Mar 28/21, Day 51 - 4L watering today with RO water. - 30ppm and 6.1pH Mar 29/21, Day 52 - cold in the tent today so turned the heater back on to brin up to 77 degrees. It was holding at 74, a little low. - nothing watered. Mar 30/21, Day 53 - 4L watering with tap water at 5.8pH - Mar 31/21, Day 54 - watered late in the day with only 3L of RO water - 25ppm and 6.1 pH Apr 1/21, Day 55 - dry out day Apr 2/21, Day 56 - light power reduced again and down to 300 watts. - plain water with tap. - 3L each 280ppm and 6.1pH Through week 8 and going into 9 now. Still some nutrient to keep working on in the leaves......give them a little more time💪

Only few pics on this week---- see ya and the first photos of eleven roses. These two clones made from outdoor plant was smaller but the resins on the buds are enormous !

8/17 Week 5 Flipping Lights set to 11-13 for the first few days then 12-12 Nuets will transition accordingly Took two cuttings for clones Reducing feeds to once a day during the switch Intro Cocotek Bloom a/b at 5ml/gal each Reduce Grow to 10ml/g each Next feed half and half 8/19 Switching to 12-12 from 11-13 think its done its work changes are afoot 8/20 Colas forming quickly may have to flip the week to Flower, lets hope so 😉 They getting a dose of Kangaroots 5ml/g and Fish Shit 2ml/g at intervals during transition purely as plants seem to need. Pics tonight 8/21 Not there entirely yet but wont be long at all Vertical growth has picked up strongly, 2" in 3 days, though I really wont call it stretch yet Continued light defoliation keeping center clear Nuets as is for now

24/01/2024 Spread her out! Time for the show. Welcome to my "Barrel Of Laughs" look what shes grown in lol 25/01/2024 Lights on and moved the pot to the left side of the tent, then sorted out the canopy with the scrog! Fed her with extra seaweed to the mix. Unfortunately, she got burnt. I made the mistake of moving the light too close, overfeeding, and overusing 12% food-grade hydrogen peroxide. Conclusion: Well, onwards and upwards! I need to sort this out. Since I used hydrogen peroxide, flushing is a bad idea. I don't want to reactivate what's settled in the leaves and soil until it's no longer active. I removed all the badly damaged leaves, likening it to a necessary amputation, but luckily I'm at the early stages of flower. I'll keep a close eye on her. 25/01/2024 Mid-day Not looking good; the new growth is the same after the flush. 25/01/2024 Lights out! Can't believe I spoiled the grow, but she'll hopefully recover. The lights, particularly the sk600 and Cropmaster, are too intense and powerful. I'm wiring up a dimmer switch to reduce it to 420 watts. The Cropmaster will be removed until maturity as having over 1000 watts of LED in a small tent is too much. 26/01/2024 Mid-day Check Time! She's looking a lot better. Taking photos is a good calf workout and lower back exercise when shooting at 0.6x at 12m. There are positives to all the negative occurrences during the growing, raising, and breeding of these plants. Weed can inspire creativity, debate, contemplation, and intelligence. It's important to focus on the positive aspects these plants can bring. 26/01/2024 Temps are more normal now since removing the Cropmaster, which was very powerful. Sleepy time: 24-25°C; Daytime: 27-28°C. 27/01/2024 In recovery and doing well. Bright moon tonight. Mid-Day: She's now ready for the full tilt. I've slowly guided the colas out and all the branches looked similar in size, which is why she's ready to grow straight up. There will be a beautiful green screen underneath! I'll coin this technique 'The SpaceProvider' as it maximizes the canopy and undergrowth space. It took me two hours to sort this tonight, and she will straighten up properly in three days. The dimmer didn't work due to incorrect volts, but I managed to reduce the light to 10% today by tapping its relay wires. Mid-day: Looking a bit better. I sorted a few branches out to rearrange and ensure they will grow straight. There are definitely no free radicals left in this plant, and the fresh new growth is a positive sign. 24/01/2024 Preparing for upcoming growth spurt during flowering phase. 25/01/2024 Adjusted the scrog to accommodate the impending growth spurt. Allowing the plant to stretch freely. Will need to reposition a few colas as needed but expecting smooth sailing from here onward. Late-day temperature check: 27-28°C. 26/01/2024 Daytime temperature inside the tent remains in the 27-28°C range, similar to pre-Cropmaster lighting. Planning to implement the Cropmaster light closer to the bloom phase as we have a way to go. Committed to only guiding the upward growth and not interfering with the flowers. Each bud requires a leaf for optimal growth. Embracing a playful wordplay: "I will need to leave the flowers alone, just a little friendly play of words." I'm looking through the window, the window to your heart! https://www.youtube.com/watch?v=1nw_ob4IeOo 27/01/2024 That was a lot of supercropping today, my goodness! Even my thumb and index fingers are aching now, but hey, thumbs up for a job well done; I'll store that in my mental index book to reminisce about later when it's all over to click through! Peaceful mind 🧠, at least the canopy will be flat, unlike my occasionally bouncy head - Slowly walks quietly away to Mediate. It looks like it'll add an extra week to the harvest date. Instead of the 8th of February, we're looking at the 15th to 17th of February for when she'll be ready, but you just can't predict with Hazes; they seem to need like 13 weeks at times. I mean, March for harvest 😆. I'm no comedian, but everyone seems to be laughing at me now. Sometimes, we have to poke fun at ourselves; it's good for the spirit 👏 🙌 ✨" "I've got two extractor fans set up, one bringing in clean air and the other pushing it out. Yesterday I noticed that the extractor out fan was off before bed, due to the weight of the cables pulling it loose. The kettle lead must have come loose, but it's all sorted now." "I know what you might be thinking; he's going to get popcorn nugs by fussing and stressing the plant in the early stages of flowering. I did mention in the article that there might be a little risk, with a pun about nuggets. Honestly, I believe I'll still yield 1lb just because of the light and growing technique used, and the extended time in long veg." I accomplished what i stated using this DIY Budmaster GoD 9 250w for the vegetative stage and 450w for flowering, drawing only 320w wall power, achieving a yield of 11 ounces. Conversely, the SK600 draws 610 watts from the wall. If I can avoid further mistakes and ensure proper feeding during maturity, I anticipate a minimum of 18 ounces. With over 80 colas, the close proximity to the light, and abundant breathing space, I've developed a unique growing technique which I've dubbed "The Space Provider," due to its ability to ensure every corner of the tent is utilized for cola growth. As a shorter version, I've considered naming it "SpaceyPro." 🤔 Bring in the reflective mylar mat for under her! *_______________*_____________*_______________* #A Brief Article Summary of This Week's Discussions & Disasters! ______________________________________________ Title: From Defoliation Disaster to Cannabis Cultivation Wisdom: A Cannabis Grower's Saga So, there I was, thinking of performing a routine defoliation on my prized Forgotten Haze Cakes, a 100% sativa strain with a long flowering period from Terpzy Mutant genetics and feminized seeds. Little did I know that this seemingly simple task would unleash a comedy of errors, leading me to discover the vital importance of precision in organic cannabis cultivation. As I lovingly tended to my plants, I reached for some hydrogen peroxide - 12% food-grade, of course - to keep everything clean and healthy. After all, research has taught me that using hydrogen peroxide can be beneficial in preventing root diseases and promoting healthy root growth. However, in a classic case of good intentions gone haywire, my well-meaning application ended up in a rather unexpected chemical reaction. Who knew that bacteria from my hands and a bit too much light would turn my peaceful grow operation into a psychedelic mishap? As I gazed in bewilderment at the unexpected brown shading on the leaves, I couldn't help but laugh at the absurdity of the situation. It was as if my plants had suddenly developed a rebellious streak and decided to rebel against my well-intentioned care. I mean, really, who needs a science experiment when you have cannabis plants with their own flair for drama? Unraveling the aftermath of this defoliation debacle, I realized that beneath the chaos lay a valuable lesson in the art of cannabis cultivation. Precision, my friends, is the secret ingredient. It's all about applying the proper measurements and foreseeing the potential consequences before diving headfirst into the world of organic plant growing. Now, about using hydrogen peroxide on cannabis plants, it's crucial to dilute it properly. A popular recommendation is to prepare a solution of 3% hydrogen peroxide mixed with water in a 1:5 ratio for watering your plants. This provides the benefits of oxygenation at the root zone without harming the plants. Timing is also essential; it's typically recommended to administer the hydrogen peroxide solution during the watering schedule, ensuring that the roots receive adequate oxygenation for healthy growth. Following this incident, I delved deeper into my research, seeking advice from seasoned growers and multiple reputable sources. The experience instilled in me the importance of thorough investigation and informed decision-making when it comes to plant care. I learned that even the most well-intentioned actions can have surprising outcomes, but with extensive research and knowledge, one can minimize the chances of such mishaps. To complement the hydrogen peroxide, I also learned the benefits of diatomaceous earth, natural and effective pest control and soil amendment. After researching extensively, I discovered that applying a thin layer of diatomaceous earth to the soil surface helps deter pests like aphids, mites, and other unwanted visitors while promoting healthy root development. To apply diatomaceous earth, lightly dust it around the base of the plants and on the soil surface, taking care to avoid excessive buildup. As I embraced the twists and turns of this growing process, I couldn't help but ponder the amusing parallels between cannabis cultivation and life itself. We all aim and miss at times, but it's all part of the growth process - mind the pun. Just as in cultivating cannabis, it's essential to adapt, learn from our experiences, and continually strive for improvement. In the midst of all the chaos, amidst the pre-flowering stage of my beloved Forgotten Haze Cakes, I found myself inadvertently learning the intricate needs of my plants. And what a wild journey it has been! I can't help but see the humor in it all. After all, every mishap has its own little nugget of wisdom, right? Now, armed with this newfound insight, I plan to pass on this treasure trove of knowledge to fellow growers and those venturing into the enchanting world of cannabis cultivation. Precision, chemical reaction unpredictability, and the foresight to foresee potential consequences - these are the valuable gems I've gathered from this unpredictable turn of events. So, to all the aspiring cannabis cultivators out there, remember - every mishap holds a lesson. Embrace the unexpected, learn from your plants, and be ready for a surprise twist in your grow. Who knows, it might just turn your next gaffe into a hilarious story to share with your fellow cannabis enthusiasts! In the end, what started as a defoliation disaster has blossomed into a valuable experience, reminding me that even in the wildest of mishaps, there's a silver lining or, should I say, a luscious green lining? In the aftermath of the hydrogen peroxide mishap, a deeper understanding of the science behind its effects on the plants unfolded. Hydrogen peroxide, often used as a root boost and cleaner during or after feeds, should be approached cautiously in organic cultivation. When used inappropriately, it can have unintended and adverse effects on the delicate ecosystem of the cannabis garden. The chemical composition of hydrogen peroxide causes it to act as an oxidizer, releasing oxygen upon degradation. This reaction can be beneficial in certain contexts, such as in soil aeriation, but if incorrectly applied, it can result in detrimental outcomes. During the mishap, the hydrogen peroxide inadvertently reacted with micro metals in the diatomaceous earth, which was being used as animal food grade. This led to an unexpected chain reaction, causing the release of free radicals and a bleaching effect on the plants' leaves. Additionally, the application of hydrogen peroxide resulted in the burning of bacteria present on and within the leaf veins. This process, similar to the effect of light burn, contributed to the browning and twisting of the leaves. When leaves are exposed to excessive light or heat, they can become brown and subsequently twist up, exhibiting signs of distress. These combined effects not only altered the aesthetic appearance of the plants but also had a profound impact on their nutrient uptake, as I had elaborated upon earlier in the article. The intricate interplay of chemical reactions and biological processes within the cannabis garden underscores the necessity for precision and comprehensive knowledge in organic cultivation practices. It is a compelling reminder that every action, every component introduced to the garden, must be carefully considered for its potential consequences. The delicate balance of life within the ecosystem demands an astute understanding of the science behind each element employed in cultivation practices. This incident, though initially wrought with challenges, has ultimately deepened my comprehension of the nuanced relationship between the cannabis plants and their environment. It underscores the profound interconnectedness of the elements at play within the garden and serves as a testament to the resilience and adaptability of the cannabis plants. Thanks for reading 📚 ______________________________________________ "A picture is worth a thousand words, so here is a thousand pictures! For the Future readers looking back on this grow 👀" - more Mental Pictures for my Dubconscious Mind 📸 ______________________________________________ 28/01/2024 "Anyway, this is it! I might need to remove some colas because there's just way too many in this 2x4 space. She'll need air circulation to grow big colas. I'll assess the colas as I start eliminating them in early flower. For now, she gets to recover and heal from the stress, snapping, and poisoning! Low light levels for a few days. Staying within 10.0DLI to prevent stretching. She'll be sprouting new pistils 🔫 next week. 29/01/2024 Mid-day: just a peek! Temps up to 29c. Should be 👍 👌 ''In The Centre Of The Maze, is the One Mother Connected too it all! That one mother that's connected to everything, in the centre of everything; like an old mob boss!" Or look at it like "The Queen Cola' City with her soldier's beside her leading them into battle" 29/01/2024 I had funny sparks for a very interesting idea for product lines. "The Adams Family Tree! Crazy Genetics from TerpyZ! " - coined it 😉 😜 Mid-day: She's looking swell this evening, took a window photo like I promised myself. She's springing back to life. Onwards and upwards from this vertigo abruptness 🙌 ' - The Stalker's Of SteMetil Prochlorperazine's The stalkers of SteMetil Prochlorperazine are here, to take the die off Elijah. Peck till I'm full, till the red dye stained blood orange is beneath her.. Having practiced for quite a while pouring my thoughts round here, whinnin' is my main feature. Loosing the hatched, despite scoring life backwards. Conquering our spectacule life fears. Under the hues of the Technical Sky Mirrors. As we gaze at the Mirrors, complementing the Maze which it infigures: The image behind this Window becomes clearer when you smear the vintage velvet. While the Brown Orange slowly fades and vanishes its hell-pit! New found knowledge is helpin', To Ground that hair-spin, that's looking all alone on this barreled mountain! A mountain formed in that of mistakes They just kept on mountin' Time to send them of on a horse and mounted, Over To a distant land, leaving behind her banishments'. Down to a 4inch scissors for cuttin' these Mother Fu#kers, taste of my own inner medicine. I debated waiting a little longer to flower her Forgotten cake then be over yarnda', I wipe the slate clean, this is over steering But she turned Queer like the average human being A beautiful layer of green resembling spears, Soon we'll be seeing, Will replace with flowers displaying such splendor and grace. Not brittle and dark and full of hate. A couple dropped off' and died What a fate! She seems spineless, her name is Mindless, 'The Queen Sophie' with such Sativa Elegance. She's round like the 'Galaxy of Spheres', and the Atom Elements. She's the round Table of Relevance that we can't comprehend; It's like we stumbled and dove headfirst into a well of mints. She possesses stable genetics for intelligent plant development; She's the last sound you hear before passing out until next year. Release all your fears, She'll help you journey back through the years. What's her name?? - 'The Queen Sophie' "Is that God? Or just me looking back on myself or not? I herd these sheep like ''Houghton heard a WHOOoo?'' . 30/01/2024 Sorted out the colas and arranged them freely as they grow naturally. One or two haven't fully healed from supercroping the other day, tied them up for support. Took off the main ties holding the stems back. Will keep a close eye on her pull on the Scrog during week 11. She's in her finally state now, ready to grow and bloom. Few of the flowers are showing signs of fresh new pistils. ''Crash & Sonic: Did a Dashing, Smashing Job Cleaning up'' Applied a fresh layer of top soil and 4 Litres of Distilled Water with Hydrogen Peroxide 1:5. Did a Spray Feed with Aloe Pro Natural Nutes 1;5. 30/01/2024 Mid-day: Spread her out more and re arranged again. I need to leave this plant alone. I will prefect this growing technique, I see a few mistakes I made on guiding her, rushed it a little. Can't wait to see the back-end of this plant though, lol. All the mistakes are my fault, not the plant's obviously, but you know how it goes in the garden. Little voice: "I'm taking stock of all the errors and looking to learn from them. I know I've made some missteps with my organic soil and feeding schedule, and it's time to address these issues before my next attempt" I think the timing of my feeding schedule and letting the soil dry out has been off, which may have led to the proliferation of bad bacteria in the soil. Additionally, I suspect that the wire I've been using may be causing the plant to react negatively, resulting in what looks like a poison-induced response on her stalks and stems. I've also noticed some open wounds from overdone supercropping on my part. Researching on Cannabis Organic Soil Making: After some research, I've found that making quality organic soil for cannabis cultivation requires a deep understanding of soil composition, nutrient cycles, and microbial activity. Using organic compost is essential to provide the right blend of nutrients and beneficial microorganisms to support the plant's growth. By making my own organic compost, I can ensure that it's free from any harmful contaminants and tailored to the specific needs of cannabis plants. Furthermore, I've learned that the soil needs to be well-aerated to ensure proper drainage and air circulation for the roots. This is important for maintaining a healthy microbial population in the soil, which facilitates nutrient uptake and overall plant health. It seems that my soil may have become compacted and lacking proper aeration, which could be contributing to the issues I've been experiencing. According to my research, the feeding schedule for cannabis plants is crucial, especially when working with organic soil. I've realized that I need to be more attentive to the timing and frequency of feeding to prevent the soil from drying out excessively, as this can disrupt the balance of beneficial microorganisms. From what I've read, trying to maintain a consistent moisture level in the soil is key to ensuring the well-being of these microorganisms, which in turn supports the health of the plants. Concerning the use of wire, I've come to understand that certain materials can have negative effects on plants. In my case, it seems that the wire I've been using may be leaching harmful substances into the soil, resulting in the plant's adverse reaction. I'll need to look for alternative methods for supporting the plants to avoid any further issues. Dealing with a Sensitive Haze Strain: I've also discovered that the Haze strain I've chosen to grow can be quite sensitive to its environment and feeding regimen. This can make it more prone to reacting negatively to any irregularities or contaminants in the soil. I've definitely underestimated the level of care and attention this strain requires, especially when it comes to feeding. This is a valuable lesson to keep in mind for my future growing endeavors. Moving Forward: Taking this step back and doing the research has shed a lot of light on the mistakes I may have made. Going forward, I plan to make necessary adjustments to my approach. Firstly, I'm going to focus on creating a high-quality organic compost to ensure that the soil is rich in beneficial microbes and free from any harmful substances. I'll pay close attention to the moisture levels and aeration of the soil, as well as refining my feeding schedule to better suit the needs of the sensitive Haze strain. In addition, I'll seek out alternatives to the wire support I've been using, to eliminate any potential sources of contamination for the plant. In conclusion, I'm encouraged by the opportunity to learn from these challenges and improve my growing techniques. As frustrating as it can be to face setbacks, I believe that overcoming them will ultimately make me a better grower. I'm looking forward to applying these insights and making the necessary changes in my approach to achieve better results in the futurE. 31/01/2024 Midnight for the plant time: I've lowered temps: 21c at night, 24C Daytime: should help will healing and recovery. I'll be checking in again next week – hopefully! See you all then! Let's go!" ___________________________________ 1. Why did the Forgotten Haze Cake weed stalk get arrested? Because it was caught "joint-handed" trying to high-tail it out of the garden! 2. What did the Forgotten Haze Cake weed stalk say to its friends at the party? "I may be a little stemmy, but I'm still the life of the bud!" 3. How do you describe a Forgotten Haze Cake weed stalk in a suit? "Distinguished, with a hint of earthy undertones and a subtle note of sophistication!" 4. What does forgotten Haze Cake say' when santa stop delivering her presents due to bad weather? "I guess when Santa stopped delivering presents, Forgotten Haze Cake thought, - 'Well, if Rudolph isn't real, he should have at least hired a fog light specialist that foggy hazey Christmas Eve!' "Distinguished, with a hint of earthy undertones and a subtle note of sophistication!" I hope these jokes bring a smile to your face as you read through this diary of 😠madness! https://youtu.be/ZttOQlqgiGA

This one is probably finished by the end of next week😎

For the most part the girls did well other than the Durban Poisons getting some rapid purple leaf on both plants at the same time. One morning temps hit 15c due to an error with my new heater. Hoping that is what the cause is. Got tired of chasing fungus gnats so I decided to fight fire with fire and be a little proactive and release ladybugs and a few million nematodes 🧐🤓. Nematodes will become a weekly part of all growing in the future. As will ladybugs just to ensure they help keeps mites and gnats at bay. No obvious bug damage so far but I don’t want to jinx it as I have 3-4 weeks left on these 🤔🤔. Check it out at thebuglady.ca if you don’t want to use sprays! Till next week!

WE ARE SO BACK PEOPLE!! 🎉🎉 Welcome welcome back to my weekly update, we got some good and some bad news this week. Bad news first: I will postpone harvesting due to the trichomes remaining clear which I am pretty frustrated about. BUT good news: we got some new light! I updated to a FC-1500 EVO from MarsHydro which got some insane efficiency compared to my last light. I can hang it 10 cm higher with a better light spread and a PPFD increase of 200 (now staying at around 900 PPFD throughout the tent) and all this while taking 10w less! Truly a technologie of all time! 😂 However I will harvest on Wednesday in the upcoming week, because it allows me to have my desired 10 days of dry-time while also maintaining my schedule with starting the Cup-grow February 1st. I also included some Bud Porn this time around because I felt like messing with DavinciResolve again, nothing too fancy though :) 14.01.: - watered all plants with 1L/plant - increased max. temperature to 26 Celsius - decreased the light to 20 cm height ➡️ all of this to boost the metabolism of the plants for them to produce cannabinoids faster and making my trichomes milkier 16.01.: - changed the lamp to MarsHydro FC-1500 EVO - height at around 30 cm while maintaining the temperature levels 17.01.: - watered all plants with 1L/plant - this is the last watering they will receive

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Week 3 Veg Update, (Please see the following) Both Plants ( Seedsman -Critical +2.0 - Blimburn (Applefritter) Thus far both plants are coming along nicely WIll be using Bud clip bender this week to utilize PPF light usage. *Mid-Week Update* Introduced CaliMagic this week LST and Bud clipped Light Defoliation Introduced Element Nutrient (Flower Fuel) 1 week until early Flower. *End of week recap* 08/21 Light Defoliation Bud Clip tie down Thus far all is running smoothly, No changes from above.