Begining week 5 of veg the Purple Goat Cheese I moving along all her tops are starting to come up after being topped an she is looking nice an healthy just a little bigger then the Strawberry Banana but a nice bush just like her The Purple Goat Cheese is doing good ending week 5 finally starting to pick up some size still the smallest tho but she's been catching up

When I was taking the weight I forgot to zero the weight with the RAW bags 😭 One bag is 32 grams, so my final weight is 168. At my next grow with these... I need to remember makes some support before they fatty up theirs bud 😄

Massive fattening this week from the Lemon AK, shes a big one. Another 2-3 weeks and she might be ready! Zkittles is maturing, but slower than i had hoped :( another week at least i think. but thats about what everyone else gets. Both of these plants are very true to their names and smell spot on. Lower nute intake seems to be working somewhat so im gonna keep it this way till the end.

Ahhrrrrrggggghhhh PH imbalances! Don't panic. We got this. After a few theories and a bunch of research what I originally thought was light stress then a Cal Mag deficiency. Then I saw leaf damage and thought of Thrips. Wrong wrong and wronger. Turns out the soil PH is all out if wack. By which I mean waaaaaay too high. Like almost 8. That's what I get for not thinking PH was nOT tHaT BiG a dEaL. Now equipped with my PH and TDS meters and a little PH up and PH Down from Standard Hydroponics we can get thing back into balance. Lots of leaf growth this week. Looks like flowering is right around the corner. This will probably be the last week I'll feed her Grow Girls Grow from DSN and switch to Bloom Baby Bloom next week. I've also done some defoliation this week to get light to some of the bud sites. Again I had no idea LST was this powerful, she's gonna have such pretty flowers Looking forward to Bloom 🌹🚀🌻😎

I experienced a 7.7 magnitude earthquake, and both electricity and internet were cut off, so my posts are delayed. We are still continuing with humanitarian relief efforts, and I am helping myself. Casualties and building damage have occurred in almost the entire city. With much sadness, I present the photos of this week

Last day of veg ( 18/11 ) all plants had a few leaves snipped here and there, plants looking the healthiest I’ve ever seen them, responding very well to the HST given throughout the week, all ready to switch 12/12 tomorrow morning!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Girls doing well this week will update soon.

Week 9 of flowering. Not a great week forecast.

Just starting to flower 😃 This week I foliar sprayed ChitoSal and Rubicon Marinis to help transition to flower. I also added nets to the rest of the plants since we've had crazy storms lately but they hole their own quite well. Roughly 30 gallons of soil inside cages wrapped in plastic about a foot and a half high amended with 3 cups Gaia 4-4-4 and 2 cups Gaia 2-8-4 and 2 cups Gaia Feather Meal 13-0-0. Topped up each month with a cup of each as well as worm casting/soluble kelp/molasses compost tea once a week. Also Fish Shit amendment added at recommended doses. Orange Kush Cake, Cherry Gar-See-Ya, Mac 'n Jack, Crescendo Rbx1 are all Ethos genetics at 25-35% THC Blue Afghan is from Jordan Of The Islands. Recieved clones in the mail in early May. Happy Growing 😌

Última Semana de Crecimiento, ya cambió ahora el fotooeriodo a 12/12 y que empiece la fiesta. Subí un pelin el juicio Grow de Advanced nutrients para que arreen el último estirón. Hice un mix de podas desde la típica apical a la fim y algún lst , os iré enseñando más imágenes pero en las fotos ya se pueden apreciar. Estos clones me están sorprendiendo están estirando genial y son bastante resistentes hasta ahora no hubo ninguna plaga. La potencia del foco actualmente está funcionando a un 50% , y ya está semana que viene empezaré aplicar abonos como si fuese floración. La semana que viene volveré actualizar , la verdad que estoy pasándolo muy bien con estos 2 proyectos. Buenos humos familia 💨💨💨

Hey everyone 😀. These weeks they have continued to develop very well 👍. They have made a huge leap forward since they were repotted 😃. They will be topped again this week, and will then move to the flower tent next week 😍. I am very curious to see how it will develop until next week. I wish you a lot of fun with the new update, stay healthy 🙏🏻 and let it grow 🌱🍀 You can buy this Strain at : https://sweetseeds.es/de/red-mandarine-f1-fast-version/ Type: Red Mandarine F1 Fast Version ☝️🏼 Genetics: Red Poison Auto®️ (SWS39) X Tangie (California Orange x Hybrid Skunk) 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

Week 5 of flower 9/6/24 After neglecting to check runoff EC, the reading was 3 and PH 4.7, it's crazy that the plant did not show any signs of damage besides one branch. After flushing the growing medium with RO water + FloraKleen + H2O2 (PH 6.5), the runoff PH was still to high. The problem persisted after watering with 50% dilution nutrient solution and still getting low PH readings and high EC. The solution was to put something underneath the fabric pot, to create a gap for the runoff water to drain freely from the bottom part. Before, the fabric pot was sitting on the plate and the runoff was draining from 2 little holes in the middle to the drain compartment. Right after creating the gap and watering, all the readings were corrected. Fortunately, all salt build up got washed away, and the plant showed no more problems. :) On day 26 days of flower, the nutrient schedule was changed to fit the fifth week of flower. Note to self: check PH and EC every 3 waterings.

All good so far, just sprayed them with anti-insect just in case (thats why the leaves looks shiny or oily) also noticed that the one i have in the right corner streched more the first week than the other 3 but that one is bigger since the beginning. Added 120g per plant of biobloom (3g per liter) and increased the light to 315w 😁

Fastbuds Meme Video Contest

These plants are exciting! 🤩 (Today is day 22 (from sprout with green). We have two plants here. Both are G14’s. Plant #1 we topped on day 11. Plant #2 we are letting it ride out to see what it does, but with a bit of help from LSTraining. Plant #1 is measuring around 7.5 inches but that’s on an angle as pictured, next time I untie it to rearrange I’ll try to get another measurement. Plant #2 is around 5.5in. Both are just doing great and changing daily! Even from night to day we notice a difference! Both are receiving fox farm plant food and calmag each watering. I removed those super big fan leaves today so I’m hoping they do ok with that. 🤞🏼💪🏼🤩 As I’ve said in my other journals, I take a lot of pics lol. I’m here to learn and to help when I can and I love to see everyone’s baby’s grow 😍 Thanks for checkin mine out! Cheers to another week 💨 💨🤞🏼🤞🏼💪🏼💪🏼🤩🤩

Day 42 | #1 is developing well | #2 is doing best | #3 is a runt | Lots of trichomes on #1 and #2 Lots of catching up to do for #3 Harvest expected by Halloween

Week 6 was smooth. Especially after correcting the signs of deficiency. The plants have bounced back quickly after being fed and are thriving in the environment. New growth shows no new signs of deficiencies, and old growth did not get any worse. Luckily it was caught early enough and corrected. At the end of week 6 I continued to tie down shoots and continue the LST throughout the canopy. Light defoliation was also done around prime bud spots, and the lower skirt near soil. Technically also removing lower growth that won't amount to much, aka "lollipoping." Plan for week 7 is to continue to water when dry to a PH of 6.2-6.6 and LST with light defoliation where needed. Monitoring the environment and running dehumidifier to 45% RH. Fans are blowing and these seem like they will be big girls. These are some of the bigger autos I have seen personally. *UPDATE* One plant is showing nitrogen toxicity. Leaves are curling bad at top. I took her out and flushed her, as I may have built up too much with run off when fixing the phosphorus issue. Continue to monitor and hope it recovers quickly. But have also seen a lot of other grows with similar leaf traits with downward curls, might be genetics. None the less, will let dry and water thoroughly all plants next time with PH 6.2-6.5 water. *UPDATE 2.0" So the girls soil was not good. Turns out my calibration was off on pH meter and was flushing with around 7 pH water. Read the soils and one plant, that had the claw and dark leaves, was above 7.0 soil pH. Others were below but still a bit high. Have been adjusting pH of water to about 5.8-6.2 pH for next few to help lower it. One girl is not happy. We will get the train back on the rail though.

Week 3 of flower for Space Panda Pheno 2 in flower: Shes showing some purpling from the cold nights they are having unfortunately. Pheno 1 in veg: Probably going to flip her to flower next week.