18.12.24 BT#53 Servus liebe Growmie's ✌️ Die Purple Punch x Lemon Drizzle von Barney's Farm macht sich einfach nur gut. Jetzt gibt es für Sie nur noch Ph angeglichenes Wasser lasse sie noch bis zum Blütetag 60 dann kommt der Cut. Sie riecht so stark nach Zitrone was mich auf den ersten Test freue lässt,generell was ihr Geschmack mir auf der Zunge bereitet. Sie ist jetzt mit dem Purpeln was die Blätter angeht auf den Höhepunkt, auch Ihre Buds haben noch mal richtig zugelegt. Die hellgrünen Buds sind überzogen mit Tricome die jetzt alle Milchig sind aber noch nicht genügend Bernsteinfarbende. Aber es sind noch 7 Tage da kommt noch was also freut euch auf das nächste Update bis dahin stay High. Mögen all eure Ladys Gesund und prächtig wachsen und euch mit schmackhaften Stuff versorgen 🍀😋 ■■■■■■■■■■■■■■■■■■■■■■■■■ 18.12.24 BT#53 Hello dear Growmie's ✌️ The Purple Punch x Lemon Drizzle from Barney's Farm is doing just fine. Now she only needs pH-adjusted water until flowering day 60 then the cut comes. She smells so strongly of lemon which makes me look forward to the first test, generally what her taste gives me on the tongue. She is now at the peak of her purple leaves, and her buds have also really grown again. The light green buds are covered with tricomes which are now all milky but not yet sufficiently amber-colored. But there are still 7 days to go so look forward to the next update until then stay high. May all your ladies grow healthy and splendid and provide you with tasty stuff 🍀😋

Que pasa familia, vamos con la tercera semana de floración de estas F.U.H. feminizadas de Seedstockers. Vamos al lío, las plantas se trasplantaron a macetas de 7 litros. El ph se controla en 6.5, la temperatura la tenemos entre 24/21 grados y la humedad ronda el 50%. El ciclo de crecimiento puse 12h de luz, el foco está al 70% de potencia. Me gustaría estar más encima este cultivo pero la salud me está impidiendo un poco estar 100% con el proyecto. - os dejo por aquí un CÓDIGO: Eldruida Descuento para la tienda de MARS HYDRO. https://www.mars-hydro.com Hasta aquí todo, Buenos humos 💨💨💨

Changed to flowering nutrients DWC changed led to 12 hrs on/off Watering every other day about 2 litres each soil pot = just ph and mammoth p Also ARE YOU SERING THIS ? These look awesome - on 80% power Soil plants always grow quicker at the start , but one r the roots are established in DWC , well you’ll see 😉 Got some serious eating going on , watered soil 2l each Tuesday

Purple plant good. Other plants not so good, herming a bit. Glad they are in separate tents. Don't grow bag seeds...

Starting the sixth week of my ladies. Cleaning the bottom and removing the « suckers » (future popcorn buds at the basis) to get more energy to the top !

Che ve ne pare? Ha iniziato prefioritura giusto una settimana fa, la prossima credo sia molto interessante. La pianta non mi ha portato nessuno problema fin ora, neanche in ambito fitofagi. È in forza e pronta a mordere e la cosa che piu mi appaga è il non usare fertilizzanti chimici o organominerali. La strada ora è tutta rosa e fiori contento e soddisfatto qualsiasi sia il risultato. Concimazione con compost autoprodotto + My banana biobloom/macerato e compostato+ algabloom a stimolazione. Grazie per la visione,per info rispondetemi.

Day 79, 28th of November 2020: Here we go! Nice little plant! Nothing bad to say great smell also. Nothing much to even report all good I am happy great genetics. Fertilization is still the same every second day with the rationand mixture above stated. The lamp is on 11.15 min and off 12.45 min. Last week was 15 min longer light cycle.... So every week 15 min shorter light cycle until the 5th week. So far -45 min. It switches on at 6 am and off at 17.15 pm.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Heute ist ein aufregender Tag im Zelt! Meine beiden Hybriden Brain Damage und High Mars scheinen sich unter den Mars Hydro FC 1500 Lampen pudelwohl zu fühlen. Die Blätter sind sattgrün und kräftig, ein Zeichen für die optimale Nährstoffversorgung durch das Hesi Hydro Zuchtschema. Der pH-Wert liegt bei perfekten 6,2 und der EC-Wert bei 1,7. Die Temperatur im Zelt ist mit 25 Grad etwas höher als ideal, aber die Klimaanlage läuft auf Hochtouren, um sie zu senken. Auch die Luftfeuchtigkeit von 65 % ist noch etwas zu hoch, aber der Luftentfeuchter ist bereits aktiv. Die beiden Ventilatoren sorgen für eine gute Luftzirkulation im Zelt. Heute Abend wird es dann noch mal spannend, wenn der Chiller für die Wassertemperatur eintrifft. Ich bin gespannt, wie sich die beiden Damen in den nächsten Tagen entwickeln werden!

👉The plant really faded fast during this week. I harvested the plant from the top down and staggered the process out over the week, fininshing today, 7 days later. The Queen Cola branch was giant. The calyxes had purple tinge too them. The queen took 7 days to loose 75% of its moisture and is now in its jar and stable at 62%Rh. I pruned and weighed each branch after trimming. I then hung each branch on a rack. After two days I put into a paper bag. The aroma at harvest was diesely. Very strong and you can smell it outside. The buds are dense and frosty. The trim wasn't to punishing because the plant didn't have many sugar leaves and those that were were covered in frost. I hung all the main branches in individually, and the secondary branches i put on a hanging net. There was minimal popcorn or larf and was not included in any of the weight. The plant was well beyond average size. For the pictures I used my cell phone. THe backdrop is a darker green. But like most cell hones the colors can be slightly skewed in the process. I did some adjustments on my computer to adjust for the color drift.

Decided NOT to water one more time. Put into the dark around 1:20 P.M . Usually do 48 hours, might just do 24 this time. Leaves are still a great color and very healthy. Took the light out of this tent. Going to attempt to rearrange things AGAIN! Spider Farmer 2x2 was just at 90 rh! Thinking of moving that light ( Alphapar) back into the Alphapar tent. Can't remember why I moved it in the first place, think it was for drying another.

Eccoci qui... Tutto va per il meglio, questa settimana non ho applicato stress alla piccola, ma ho guardato l'evoluzione che ha avuto dando i nutrienti. Ora ha un odore davvero intenso ed uno strato di resina pauroso, vediamo come si riprende dalla defogliazione che ho fatto settimana scorsa. Manca poco, settimana prossima controlliamo i trincomi. Seguiranno aggiornamenti, grazie a tutti per il supporto🔥🌲❤️

Starting to stretch a lot 3 plants 32 in the rest about 24. Have about 2 feet left until max light height. Do you think they will get that tall?

Hey people. So far everything looks good. I highly doubt that any lady here will be as productive as one of my “Runtz”. But we'll see. Have a nice week everyone.

Hello everyone, my friends! this week we will do more with the rinsing of the roots, waiting for these magical nuggets to swell even more 😉🍭