2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Day 36 she keeps stretching and looks very happy i'm also very happy because soil PH is finally good, now it's 6.5PH after watering with 5.2 PH'ed water(with nutes in it) for the last several waterings. Light is 30cm away from the canopy ~850 PPFD Cut some leaves from center of the canopy for better air circulation and light penetration. Day 38 : watered with 2.5 L of water with nutes and 5.6 PH water, runoff water PH is now perfect 6.5 Day 39: She's been stretching very well, can't wait to deflate already(maybe tomorrow) Day 40: deefoliation day finally has come! And i'm so happy how she turned out after all. I did some LST to even out canopy for better light penetration and air movement! Note: she uses much Calcium - Magnesium (cal-mag) soo you definitely need to add it in your water.

Week ended on flower day 19

End of day 35 flower Had an issue with cal/mag deficiency with the White Widow so I had to flush her and increase cal/mag to 5ml/gal. So she should be back on track to produce fatter buds. Royal Gorilla isn't fattening up so hopefully she will this coming week. Also gave her a good flush with some florakleen. Defloiated so the buds on the bottom can get more light. Thanks for taking the time to check on my grow. Peace out!!

Ya visteis , cultivo fácil, de principio a fin, pocos errores una pérdida tan solo de 4 ejemplares y por dejadez. Pero enserio si todo se hace correctamente vais a flipar lo rápido que van y después lo buenas que están. Humedad en el secado por debajo de 45% y temperatura media de 23 grados. Hasta aquí es todo fumetillas, que paséis un final de año de lujo entre buenos humos.

Very easy to grow, excelent yield (could be better but because of the grower poor experience hehe). Makes you euphoric and very very relaxed. Is excelent for day and night use after a hard work timeoff.

Day 91 - Fed lady 1 with water amended with only Cal-Mag. I ended up cutting off her lower colas that were dying due to not getting any light. Lady 2 is fading more and more everyday. Her trichomes look cloudy with the occasional glassy and amber trichome. Still thinking she's about 5 days away, waiting for her to fade more as her trichomes look good now. She's starting to show purple colors, which is awesome. I moved lady 1 directly under the light now to take full advantage of the mighty 100W (/s) light. Lady 2 will finish in the tent with indirect light. Day 92 - Lady 2 is getting closer and closer to harvest day. I'm thinking less than 4 days away now. Five of her 8 big colas have now either faded or started to fade, so once all 8 are in some phase of faded I will chop her down and begin drying. I'm expecting her to dry in about 7 days. Hopefully I can keep the planned drying closet between 50% and 70% humidity... Day 94 - Decided to chop down lady 2 today. She was on day 70 of flower. Fed lady 1 with water amended per this week's diary. Day 96 - Took some leaves off of lady 1. Day 97 - Fed lady 1 with water amended with only Cal-Mag.

Will grow again for sure shout out to @420fastbuds for the strain what a all round great plant!!!!

un poco de lst para ir probando

Apollo is in early flower. I water and fertilize Tuesday, Thursday and water on Saturday or Sunday. Even though the plants started within 2 weeks of each other, Apollo is in flower now. The Vpd for veg is much higher than for flower. So on 10/17, I moved the Friends ( Zkittles, Godzilla Cookies and GrandDaddy Purple) to my 3x3. Stress training done on friends: Zkittles -tie down GDP -topped Gorilla cookies no stress training as this plant is the runt of the friends. Pics of Apollo's friends before moving tents. Friends, Royal Queen Seeds Is offering a discount on purchases 👉**GROWERS20** for 20% 👈 Https://www.royalqueenseeds.com Link to Apollo https://www.royalqueenseeds.com/us/56-f1-hybrid-cannabis-seeds Variety: F1 Hybrid auto Genetic Background: Lemon x Black Domina x Sugar Mangolia THC: Very high Yield: XXL Height: 55 - 75 cm Flowering time: 45 - 50 days Harvest: 75 - 80 days after sprouting Effect: Calming, Creative, Motivating , Physically Relaxing Flavor: Citrus, Herbal, Lavender, Pine, Vanilla Major terpenes: Caryophyllene, Farnesene, Myrcene, Terpinolene Climate: Short Summers ==================================== Equipment: AC Infinity CLOUDLAB 844 – Advance Grow Tent 48”x48”x80” CONTROLLER 69 PRO – Grow Tent Controller CLOUDLINE LITE 6 - Inline Fan 6" IONBOARD S44 – LED Grow Light Board 400W CLOUDRAY S6 – Oscillating Circulation Clip Fan Carbon Filter 6” Pots: 5 gallon Air pots Soil Fox Farm Happy Frog amended with worm castings, dolomite lime and mycorrhiza. Seeds provided by Royal Queen Seeds Check them out 😎 Https://www.royalqueenseeds.com Link to the seeds https://www.royalqueenseeds.com/us/56-f1-hybrid-cannabis-seeds use Discount Code 👉 **GROWERS20** for 20% discount 👈 Nutrients: Total Plant Solution >TPS1 >Canopy >Signal Amended soil recipe #2 used for soil prep. https://www.paramountseedfarms.com/super-soil-recipes/

2024.09.12 D64 Its been two busy week, as i trim the 1 pond mother, get aknowledge that my wife use my grow sniper two pruned is badly infested rose bush, since 2 week and never proper clean it whith alcool.😱😱😱😷 in result a massive PM & mithe contamination in my grow tent... 😱😭😷😭 all plant have been shoot with H2O2 at 0.5% and set ouside for 2 day, wich turn to be rainy as f***, until i clean and serilized everything, twice and run the hepa filter .😷😰 the plant look ok, lightly over wartered, but no more pest or PM sign as they return in the tent.. 3 days later PM hardly bonce back, no choice to use more agressive treatment so they all have been shoot with saffer's defender 3 in 1. after 3 shoot in 24h and another tent desinfection we should have get rid of it 🙏 8 day laters everything look fine👍, all damage leaf have been removed, i also prune some unrelevant lower and inner grow

Everything after germination has been pretty smooth. The plants in veg had a couple small brown spots due to under watering but no extreme signs or any abnormalities.

What can i say about this amazing success story from Barneys Farm. They were a breeze to grow and took whatever i theew at them without a hitch. the bigger pheno girls were amazi g with growth spurts and went easily more than double in stretch. I ran out of silicon and calmag which did hold them back a little during flower. In true Gorilla strain style , they were floppy with their weights from about week 6 and i suggest staking early on for a better control of their bud position. Without my lazy growing practices ( being resolved for the fast bud run shortly !) , i feel this strain is a winner. rhe smaller phenos were unfortunate but still gave nice buds to smoke. I would recommend this stesin to growers at all levels. easy if you want it to be but would meet you every step of the way with more complex training methods too. Love Gorilla Skittelz. Thanks for sticking around and commenting. Would happily share a bowl with you all.

What a awesome journey !!! I love this strain in every possible way, its so tasty !!! 295g DRY WEIGHT !! thats crazy, love it ! Will be growing more FORBIDDEN RUNTZ without a doubt soon ! Papaya Cookies and Strawberry Gorilla from FASTBUDS will be the next diaries, please follow 😎

Diese woche war ziemlich stillstand. Denke auch das sich nichtmehr viel tun wird. Tippe das sie in ca 3 wochn fertig sind. Werdn noch ein bisschen an gewicht zulegen denk ich. Aber viel abwerfen werden sie nicht. Sind aber ideal für extrakte. Sind ab tag 14 komplett mit resin überzogen umd wird von tag zu tag mehr.

Final stretch, let’s hope I can figure out when to harvest at max potency.

It's a snow storm going on in this tent! Just luv how frosty the buds are getting 😀

Jetzt war für mich der optimale Zeitpunkt zum toppen. Sie hat es gut verkraftet, was man auf folgenden Fotos sehen wird. Ich habe versucht so gut wie möglich im 45 Grad Winkel zu schneiden. Aus allen Cuts mache ich aktuell Clones. Ich liebe Clones aus den Main Cuts. Daraus mache ich dann Mutterpflanzen. Die Mutterpflanzen von den besten Phenos bleiben.