Última semana de fertilizantes, una semana aprox ahora en lavado de raíces con Fawless Finish ✌️🏾

90x60x140 (Mars Hydro) FC-E4800 (Mars Hydro) Easy2go Aquavalve5 (Autopot) Living Soil (Demetearth) Bruce Banner (PEV Seed) Gorilla GG4 (Ganja Farmer) Cream Caramel (Sweet Seed) Gorilla Gelato (Ganja Farmer) Blueberry (00 Seed) Kalini Asia (Zamnezia) Biscotti Mintz (Barney's Farm) Blackberry Cake (Sensi Seed) Amsterdam Amnesia (Dutch Passion) Gelato Cookie (Ganja Farmer) Purple OG Punch (Ganja Farmer) Sweet ZZ (RQS) Rainbow Road (Paradise Seed) Substrat ~50L: 30L Super Light Mix Biotechnologie 18L coco 2L perlite 1,7kg lombricompost 350g guano de chauve-souris 350g Zéolithe 350g Basalte 170g guano vers de farine 170g biochar 0,7g Endomychorise glomus intraradices 0,7g Bacillus Amyloliquefaciens Paillage de luzerne alfalfa Arrosage avec 2ml/L de mélasse de canne 1x par semaine Pulvérisation avec 1% d’huile de neem jusqu'à la 2eme semaine de floraison

The smell is strong, healthy and fast growing.

Did some defoliating. ✂️ Hopefully they're done stretching. ⬆️ Fed them recommended nutes 💉 Moving happily into the next week.😊

New week. Started feeding 2days ago. 2part instead of 3. Managing till i get the last part in. Roughly 2weeks. Currently using. micro and bloom from advanced nutrients. Currently waiting for roots to show under the rockwool then i can move it into the 5gallon bucket.

Die Pflanze wurde Mitte letzter Woche an Tag 66 geerntet. Getrocknet wurden die Blüten 6 Tage lang bei einer Luftfeuchtigkeit von 53% bevor sie nun ins Glas kommen. Getrimmt wurde die Pflanze trocken.

Good evening to all growers :D! This week was a bit disappointing when I discovered 1 male plant, immediately moved to a separate box (I'm not a killer)... The two bad girls are showing their sex with pistils that are now visible, the largest is very evident while the other with the paint bucket pot has yet to develop... Odor for now nothing but I still have good intentions from :D!!

Día 22 de vegetación, se realiza poda FIMing a las 4 plantas, que ya tienen 6 nudos, se ven fuertes y de un color verde muy sano, se riega un día con 250 ml. y al otro con 100 ml. se va variando entre agua con calmag, microvida o fertilizantes para mantener baja la EC. No estoy corrigiendo el PH como parte del experimento. Día 25 de vegetación, las plantas se han recuperado bien de la poda FIMing, se riegan a diario y cada riego con fertilizantes diferentes o solo calmag, para mantener una EC bajo los 800. Se siguen doblando las ramas y podando los brotes inferiores.

Erste Blütewoche: Tag 55: Ich spreche quasi aus der Zukunft weil ich recht wenig Zeit hatte in den letzten drei Wochen…aber es wird wild 😝 wir sind aus dem Urlaub zurück und die Pflanzen waren gut 65cm groß, höchste Zeit für den 12/12 Switch! Und natürlich je 4l Wasser für jede Pflanze mit ph 6.2 und 1ml/l Calmag Agent. Ab jetzt heißt es erstmal die Pflanzen in Ruhe zu lassen und die Beleuchtung der Lampe auf 270w von oben und 40w von unten, um das Defizit von 6 Stunden auszugleichen und evtl den krassen Stretch zu reduzieren. Tag 56: heute 3l Wasser mit ph 6.1 und 0,5ml/l calmag agent (2von unten 1 von oben) Tag 57: heute 2l Wasser mit ph 6.2 von unten Tag 58: heute mal Ruhe 😊 Tag 59: 4l Wasser mit ph 6.2 und 0,5ml/l Calmag Agent (3 von unten 1 von oben) Tag 60: tote Blätter abgepult und weggesammelt Tag 61: 4l ph 6.1 von unten pro Pflanze Ende Blütewoche 1

These kush are really beautiful and have huge peaks, they have 13 internodes and each of them is full of buds, quite large. They are really huge touch the ceiling, maybe one is too close to the lamps and I'm afraid you burn a little bit

This is 1 of 2 clones named trying to start off of the mutation area of the pure ice cream I am growing. Hopefully it takes and grows well. Plus hopefully it carries the mutation of the color with it. Thank you Pure Instinto seeds. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

First week went perfectly. Environment stable, good growth for first week. Co2 going in tomorrow and a humidifier going in as well.

Hello my friends, ...June 7, 2022.. ..Day N°87... ...Flowering day N°31... My two Feminized Royal THCV are fine and beautiful, they are monster plants, the stretching seems stabilized and the flowers smells awesome sativa. #1...140cm #2.. 145cm I give them water with a tablet of Easy Micronutrients from RQS Organics Nutrients. They are under a MarsHydro TSW 2000 at 70% of power and at 20cm of the canopy. www.royalqueenseeds.com www.mars-hydro.com www.marshydro.eu Thank you very much for passing by. Wish you the best with your green projects, peace. See you soon 💨💨💨

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Divine Seeds 2025 Auto Contest 👉Sponsored Grow👈 AK-47XL Auto W6F2 The weather was rainy with thunderstorms early in the week becoming hot, humid and overcast. I fertilized with I49 grow 1 tbsp/gal & 2 tbsp I49 Flower. I added this as a preventive measure since I’m seeing issues with nitrogen usage in the lower leaves even though she is in super soil. AK47 must be a heavy feeder. With the extreme heat and rain, my AK-47xl. She grew a foot and is now 31 inches tall. She demonstrates resistance to fungal diseases and bug damage. In the background noise on the video is Cicadas. When they hatch, they crawl onto a tree or plant and molt. The adult has wings. This is the empty body casing. This year there are some but not a lot as in many years of their 17-year cycle. As always, thank you all for stopping by, for the likes and most of all growers’ love and support. Stay green, growers love 💚🌿 💫Natrona💫

Esta semana dei a primeira poda top! Na planta #3 e #6 tentei dar a poda FIM (primeira vez que tento esta técnica vamos ver como corre)

Läuft prima. 2 fat banana legen sehr gut los. Hab sie entlaubt, damit sie in der Blüte Sonne an den richtigen Stellen bekommt und sie gut belüftet bleibt. Auf den Bildern noch mit Laub. Orion macht weiter ihr turboDing ,ich füttere sie jetzt mehr.sie will es und ich glaub, bei dem Wachstum brauch sie es auch. Also läuft mit den verbliebenen mädels🙂 bis nächste Woche ✌️

These ladies continue to develop very nicely and lucky stopped stretching pretty much in the beginning of this week and the buds are forming super nicely. Since I also don’t observe a stretch in flower anymore, I decided to feed pk13/14 for the next week. It continues to surprise me how hungry these autos are, much hungrier than my glookies fro example. Unfortunately I realized that the plant on the very right of the box produces much smaller buds than the other two. I am pinning that on the fan right above her, so have pointed it slightly higher, but I guess the damage is done:(.