To update, just keeping photos up to date...

Quite the week, finally got to transplant into a larger container, hopefully the roots open up and explore soon. First round of LST completed as well.

I managed to turn the negative heat temperature in to something positive by introducing co2 to the environment. As a result of the diy co2 method some of branches grew up by 1.5 inches in 2days! Will continue to utilise co2 in the box for now. I also had to adopt the defoliation method Way to many leaves could cause problems by raising the moisture level in the box. As a result of the defoliation a lot of light is reaching the bottom of the box. Which means I can grow other small plants in the box. I switched to bloom NPK ratios. Slowly introducing the raw mix. It’s doing fine and as allways no tip burns

🚨 Durbin Thai/Cinderella 99 week 12 update 🚨 🚨 week 12 of veg!! 88 days old!! 🚨 So this is a clone I received on Jan 31st. She has been transplanted from a solo cup 2 a 5 gal pot. She had 2 be topped in her second week of rooting do 2 the top frying from to much light in young clone stage. Since the topping this lady has come 2 life!! She has been defoliated 4 times! Most recent defoliation 3/24! She spent her first month feeding just off nutrients in fox farm soil. On 3/2/22 I gave her her first Gaia Green trio feeding!! She has exploded with growth since!! She was defoliated on 3/27 again. She was given her second feeding of @gaiagreenorganics on 4/02. This lady was defoliated on 4/10 and 4/19. This is what she looks like today!! 1 week till light flip!! My wife finished the Alice pot this week and she is now in Wackyland!! 💡 Grow Sponsor💡 @marshydroled__amazon @marsnier #marshydro #TSW2000 Marshydro equipment-- Marshydro- 4x4 grow tent Marshydro- Tsw2000 Marshydro- 6" inline exhaust fan with digital temp and humidity sensor Marshydro- 6" carbon filter Marshydro- 6" Black duct line Other companies in this grow- @foxfarmsoilandfertilizer - 100% ffof @gaiagreenorganics @inkbirdofficial - WiFi temp control @madmanplant- 6" Tent shelves Thanks 4 checking out my grow!! Till next week!!! Best of luck and Happy growing!!!

a plant very resistant to everything highly recommended...uff .... a knock out plant an earthy taste and pine ... with a dense smoke ...... and of compact and abundant buds ... a 4x4 all-terrain plant ... 100% recommendable ... with their healthy roots....00

I found seed foeming and bananas so i decided to cut my losses before it got out of hand im just glad they got to where the are now first time grow first time using dwc and i fucked this shit up for a first timer you beauty

Follow my grow to see full grow cycle. Happy with what I learned during this run. Only going to get better and better!

🤩🤩

Coming along, got the temperature down by moving the schedule more into the night and morning. Hopefully have it down enough to get some colour towards the end!

Ende BW 3 sind wir nun. Die Blätter sind etwas matt und die hatte Lichtbrand, davon hat sie sich jetzt aber erholt und fängt an schöne Blüten zu bilden. Es gab noch etwas zusätzlichen Blütendünger von Wizzy's Garten aufs Substrat, sie wurde ordentlich entlaubt und lollipoped und jetzt kann es weitergehen.

Hello friends hope all is well. Can’t say i can’t complain this tree is doing well. So far am waiting for to stretch some more. The buds are forming well. I’ll be giving her a organic compost low in N and big in P-K. So far my organic grown has been the best way to go. It more fun, and the learning experience is great to use with other gardens and plants.

Buenas a tod@s... Sexta semana de estás green poison de sweet Seeds.. de momento va todo en orden, la planta ya se hace notar con distintos tonos de verdes, olores y sobre todo resina... Van creciendo muy bien, está semana fue todo tranquilo... Con ganas de ver la etapa final y desgustarlas aún que todas las etapas se disfrutan, la final es la más power y ahí realmente ves si necesitas cambiar algo o no, de momento todo más q bien 🏻. Buenos humos para tod@s.. ⚡⚡⚡🤘🏻🤘🏻🤘🏻⚕️😎 🇦🇷🤝🏻🇪🇦

7/11 Got half in of rain last night. Glad I didn't water. Ph of rain water is very acidic. Added supports to the blueberry cheese in the 50. I just used string and went diagonal and attached to the cage. Wrote a ton but it disappeared. Anyway looking at previous diaries I was wrong about senescence. It wad the life cycle of those earwigs that did that to my plants (see other diaries). This soil mix is amazing. These plants gave only gotten like two small feeds of big bloom. I showed my commercial buddy and I'll keep it between us what was said but it made me feel really good. I'm considering either expanding the cage in one direction with some lumber I already have or moving a couple outside tge cage. That way I have more room. He said he's seen plants structurally similar reach huge heights (14f) so I'm just trying to avoid future problems. I at least need to get the trellis on. I hope poor that's food enough. Did a real nice video this morning but nothing wanted to upload and it just uploaded the same one twice. I'll try again and hopfully it will go up. If not I'll put it up tomorrow. 7/12 Dad has surgery this morning. I did a quick video and took some pictures. I need to do some rearranging with the plants and cut a pallet to fit in the back. Then I can put a vertical trellis up. Haven't decided if I'll add on to the cage, remove a couple plants or just rearrange things but I'm leaning towards the later. They will need water again and I'm wondering if I should start nutrients but everything looks so good. By this time on past diaries I'd be losing all my leaves bu now. MI think it wad part ear wigs and part to many nutes. This soil mix is taking these plants through veg. I'll probably do a feeding soon but it will be organic and it will he small like a big bloom or ancient amber. Im leaning towards not using growbig this year as my plants seem to be doing great without it. We'll see. I'll keep this updated. UPDATE: Went back over and gave plants a full watering. Some were slightly drooping. I was going to add nutes but decided against it as I didn't see any deficiency. So far NO Earwigs! I comed through the plants and I did find a jpn beetle which I happily killed. I mixed up 8 gallons of water and gave it to the 11 plants so it wasn't quite a gallon a plant. I need to rearrange the plants so I have room to move around. I also need to cut another pallet and use the spaces I'm not. Trellis needs to go up. 7/13 I think I've got the watering amount down. Now just to find how often which will depend on weather. I've watered very little this year. They loved that gallon. I was going to use big bloom and kelp me/you but looking at my garden I decided not too. My buddy asked what i was addingvthings for and to wait fir what i added to do what I wanted it to do. I see no nutrient deficiencies so why add anything? I think this soil mixture will get me all tgexway through veg. I dont plan on using much in fliwer either. Definitely good genetics. I really need to cut that palley and move the 1 10gallon to the far back coener. It will open thibgs up so much better. The garden looked so beautiful this morning. Getting very aromatic. I started untangling trellis netting but had to leave. I'll update what I do. Happy growing folks UPDATE: Went back over as I had a slight intuition that I needed to check the garden. I found and killed at least 7 jpn beetles. That's what's been making holes. They tried to escape and bounced off a tarp but I got it. I went through the interiors and found two pillars. If these beetles are gonna be around I'm ordering a net. I'm also close to positive I'm going to extend my cage in the back a few feet. Things are getting unmanageable in here. My buddy said I'll be having problems soon if I don't do something. I'll document what I decide to do. 7/14 Found ONE jpn beetle in the garden this morning. I can manage those well enough. The birds help too. I check my garden multiple times a day so I manually remove many pests. However I've noticed some thrip damage on a leaf and a leafhopper damage on "A" leaf so there are "some" pests around but not enough to spray shit. A couple wasps were doing there job while I was there. Supposed to get thunder storms after three and tonight. Supposed to get over a half inch of rain tonight. I lifted the bags and decided to hold off and let mother nature take care of it as the bags weren't totally dry. Only problem I really have is space. I AM moving that 10gallon (that's the same size as some 20's) in the back. There's 27in not being used and a few feet the other way. My buddy cautioned me that I'm going to have problems since my plants are so crowded. I agree with him. I spoke with me father and we have most materials to extend my cage four feet in the back. I think that's my plan. I'll extend the structure before the stretch then I can put up the supports. We'll see how this goes. UPDATE: Went back over to check the girls as I had a feeling I ought to. When I got there I saw that a couple of the blueberry cheese were pretty light (liftng the smart pot) but the others seemed to be fine. ONE 10th planet was light like that and the purple punch in the 10 gallon was as well. Each plant thar needed it got at least a half gallon of water. I'm waiting to see if we get the thunderstorms and the half inch of rain. I watered the MASSIVE blueberry cheese in the 50 but I only gave it 1 pitcher which is like 1/4 gallon or so. Don't know why I even gave it that. Looked fine but the soil WAS pretty dry. Next year I'm giving myself way more room. I was running trying to chase these jpn beetles. This time I have the dawn and water and a measuring cup to knock them in. This ain't my first rodeo. I did notice some bright yellow streaks on a leaf edge and I'm hoping it's not septoria. I doubt it but I have an anxiety disorder and I worry. I hope I can get the cage extended sooner rather than later. It's getting hard to move in there and more importantly I can't take any more plant pathogen problems. I'm considering starting a plant doctor regimen just to be safe. 7/15 Got a bunch of rain last night. No jpn beetles in the garden and not really much damage. I did notice this (I think it's leaf hoppers) that leave those dots close together on a leaf so it appears I've got a variety of pests. I'm considering how to approach this. I mean the damage is very minimal but I don't want it to get out of hand. Another thing I need to look out for is leaf septoria or any other fungal pathogens. I believe that has been part of my problems in the past. I think that's what caused my earlier grows to drop all their leaves so quick. I think I'm being overly cautious but its very crowded in there. With my father just getting out of surgery the girls will need to wait a little bit before before I can extend the cage. I could still cut the pallet and move the 1 10gallon and that would give more room. I rearranged a LITTLE BIT so they have a little more room but I've really got to get this cage extended. UPDATE: IT Rained so hard I had to pull the car over. We hydroplanned the whole way home. After working ob my house I went to see what the damage would be like. NOT A SINGLE BREAK THAT I COULD SEE. I took a video but since the wifi here sucks I'll have to upload it tomorrow. Walking around in the cage even if I cant get it extended I think I'll be OK. It obviously will open me up to lots more issues but at the very least I can reorganize before I build on. The 10 gal purple punch would fit perfectly in the back and I have a pallet I can cut to fit it in place. Putting that one back and pulling the others forward will be much better than what I've got going on now. I'm also thinking about running an extension cord and putting fans under the canopy or at an angle to keep the wind moving. Just thinking outloud. However after that storm the girls looked as happy as I've ever seen them. All happy praying to the sun, thankful for the much needed rain. Mother nature does a pretty good job with out me messing with it. I've noticed a couple interior lowest leaves turn yellow and die like a nitrogen deficiency but everything else is fine. Also noticed a leaf that looked like a p deficiency but again, it was the VERY bottom leaf on ONE plant. Again the rest of everything looks fantastic. I'll keep an eye out for anymore nutrient deficiencies and if anyone that reads this sees some please let me know. I should've taken stills since they looked so good but I got it on video. 7/16 It POURED last night and throughout the day. TORRENTIAL rain. The branch breaking sheet rain that us outdoor growers learned to fear. My plants aren't trellised currently. I know what I need to do now. I have a pallet to put in the back corner and I'm moving the purple punch in the 10 there. And pulling others forward where there's more room. Then when I extend it (it's gotta be done this week) everything g will be in their proper place and I can just throw on a vertical trellis. I also noticed more (leafhopper) damage on a leaf. Different leaf of the same plant so I'm considering spraying something. I have a number of products but I was trying not to use them. Luckily I have these diaries so I can look back and see certain plants reactions to certain nutes or fungacide/insecticide/nutes and the doses used. I haven't been using much but if my plants will remain cramped I'm going to start the plant doctor. I'm seeing more pillar damage too but BT is super narrow so I'm thinking when I fo spray for pests I might use cap jack and be done with it. Then I can apply the BT in flower if it's necessary. I took a video but I have to wait until tomorrow to upload. I took a quick snapshot though. 7/17 Despite the torrential rain I don't have any breaks. I'm noticing more pest damage though. Another leaf on the same plant had those closely shaped round circles. I forget what pest it is but it's there. Caterpillars are there I'm sure so I may do a preclcentative spray. Just unsure what I'm going to use. I lost a COUPLE very bottom interior leaves that look like they just got used up. After this rain I think think the plants might benefit from a feeding. Probably next water after they dry out. I REALLY need that cage extended. I expressed that today and it should be done this week. I'm looking for pallets today. I have the little one that I can put in the back which will allow me to move the 10 gallon and move the other forward. That will help some but I need more room. I'll update as I go. UPDATE: GOT A SMALL PALLET AND IT FIT PERFECT IN THE BACK ROW. I MOVED THE PURPLE PUNCH IN THE 10 GALLON ONTO IT. I SHIFTED A BUNCHVIF THINGS AROUND. I ROTATED ON BLUEBERRY CHEESE 180 DEGREES SO IT WOULD FIR BETTER. CROP ROTATION IS GOOD ANYWAY. I TOOK VIDEOS AND YOU CAN NOW SEE THE ROWS MUCH BETTER. 2 WITH 3 and 1 WITH 4. IT'S SIGNIFICANTLY BETTER THAN IT WAS. I CAN GET AROUND ALL SIDES OF EVERY PLANT NOW. GRANTED THE LARGE 50 IN THE BACK IS GOING TO HAVE SOME TROUBLE BUT ILL JUST STAKE IT TO THE CAGE. IT WILL GROW TOWARDS THE SUN ANYWAY. IM SUPRISED I DIDN'T LOSE A BUNCH OF LEAVES AFTER THIS RAIN. MOVING THINGS AROUND AND LOOKING ON THE INTERIOR OF PLANTS I FOUND A COUPLE LEAVES THAT HAD BEEN USED UP. I REMOVED A COUPKE LEAVES THAT HAD DONE THEIR JOB. I'LL UPDATE AS I GO ALONG.

All is going well!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

3° settimana di fioritura. Il profumo che emanano è già bello forte... immagino che l'ultima settimana sarà concentrato di aromi dolci e fruttati 😂🤣👍💪✌️🙏

Week 7 is going smoothly so far. The flooding issue has subsided and the clones seem to be really taking off now. I'm excited to get back from vacation and really see my girls and my reservoir conditions after a 2 week hiatus.

The girls are moving along nicely just watering with water ph to 6.4 this week as I top dressed with Gaia green 4-4-4.Spider farmer Se7000 dialed in at 30 percent and to the ceiling.Noticed some minor deficiencies Day 12 and check soil slurry 50/50 soil/water and the ppm was at 780 lil high at this early stage and the culprit.Going to work on lowering that a bit this week.I did add a top layer of fox farm ocean forest as well which runs a little hot with nutrients rather than the pro mix.I have to remember with these beautiful auto flowers less is better :)

Georgeus strain smell taste energetic and beautiful buds with bloody red with tricomes snow 😎🙌🏻