Today I fertilized the last time and for me it is always like a little before "graduation", no plan 😁 Well in any case they smell really very good and intense and there are still 2 weeks until harvest, I think, but everything works well I think I always leave the trichomes up to 50/50 tires anyway 💣😁🤤 Otherwise I will defoliate the week that comes and remove old leaves that are more than 60 percent broken depends on how they look. Until then, have a nice week😁👍💪

She´s smelling beautifully this week. Its pungent odor is getting stronger and buds are really starting to get covered by trichomes. Sticky af Day 73 - Feeding today. Will increase Atazyme by 1ml/L and gradually increase big bud up to 2ml/L (Today i fed 1,5ml/L Big Bud). Also will gradually increase Top-Max up to 4ml/L.(today i fed 1,5ml/L) Day 76 - Has been very hot these days. Today i arrived and soil was completely dry, but the plant was ok. She´s starting to show beautiful colors, lets see how this pheno flowers. :) I fed her today and just increased Top-Max from 1,5ml/L to 2. Day 77 - She is getting really colorfull and beautiful. Buds are growing great, i dont see any complaints from her. I am removing dying leafs to help light reach every bud. Has been very hot these days, at night aswell (≈22ºC).

Week 5 starts with recover from the previous FIMing. I'm surprised 😍 ------ Day 30: Found a better way to LST, the branches are growing much faster now :D Day 34: Sex clearly visible 😁 (female)

Das ist Weltklasse. Damit könnte ich international an Cups teilnehmen.

Flowering is coming along well.. each plant is starting to show signs of frosty fan leaves and the bud sites are starting to swell. I also took a video of both

Hi everyone :-) This week a lot of nice things happened in the flower tent :-) Everyone looks super nice, and is growing stronger and more beautiful week by week 😍👌. The blue cheese smells like a dream ;-) As usual from this variety 👍. The kosher tangie is also very, very tasty 👏🏻. Both got Pk 13/14 this week for the last time :-) Everyone else is developing very well 👍, That will be the last diary with several strains together :-) In future everyone will come individually 👌. I wish you a lot of fun with the videos, have a nice weekend, stay healthy 🙏🏻 and let it grow 😎👌

16.2.25: I decided to check out the lower canopies of all plants to see if I need to get rid of any foliage. I did get rid of a few small branches and leaves. However, whilst I was doing this, I saw more garden pegs from my LST remaining. There were about 6 or more in Pink Mist alone. Additionally, on Watermelon, there were some left in, too. I'm so annoyed to see that because the plants are really stretching, and I could've potentially disrupted this by leaving the pegs in for all this time without realising it. 🤞 that I haven't compromised things too much. We'll see. I watered today with 2ltrs of dechlorinated water PH'd to 6.3 containing the following nutrients; ♡ .8g Green Leaf Nutrients PK booster ♡ .5g Ecothrive Biosys I ordered quite a few things for the garden. I got Greenleaf Nutrients Sea K(elp) and Mega Crop Parts A+B. To go with their PK Booster I got last month. I'm excited to try it all together. Next run, maybe just using these. We'll see how it goes. 18.2.25: The plants are going crazy for water! Everything is getting used right up so fast! Today, I decided to add some more Black Strap Molasses to add some carbs and other micronutrients. I'll add the jar with the label in the photos section above. I watered a very small amount to each plant. What I put in: ♡ Black strap molasses 150g ♡ 2g Sea K(elp) Greenleaf nutrients. I dissolved everything in 4ltrs of dechlorinated water PH'd to 6.4. 19.2.25: I received the majority of the garden purchases that I made. I'm still waiting for the Ecothrive Life Cycle. I wanted to top dress, but it's been delayed unfortunately. I am using my Greenleaf nutrients products which I bought on Amazon. I got the Mega Crop 2 part system Part A and Part B. I have the Sea K(elp), and the bud explosion PK booster. I really wanted to get some of their sweet candy asking read many positive reviews. Unfortunately, for me, this is unavailable to buy currently. So that's a little disappointing. I needed to do a good watering so when my nutes were delivered today, I got excited 🤗 I watered 2ltrs of dechlorinated water per plant, PH'd to 6.4, containing the following nutrients: ♡ 1g Mega Crop Part A ♡ 1g Mega Crop Part B ♡ .5g Sea K(elp). The plants drank this up within a few hours. I'm going to try and hold off on watering in hopes that my Ecothrive Life Cycle will arrive so I can top dress and water it in then. 20.2.25: My Ecothrive Life Cycle arrived yesterday, and the plants are ready for their top dress and a good watering in. I have some Biobizz Light Mix, Canna Coco,and perlite. I'm going to use this as a base to mix my amendments in. I'm going to fill my 5 gallon bucket with about 4.5 gallons of my top dress mix. I will distribute this across 6, 4-5 gallon pots. Then I will water in well with Greenleaf nutrients Mega Crop Parts A+B and Sea K(elp). I've made a crude attempt to video mixing my top dress. Don't listen to the audio. lol, my YouTube didn't stop playing whilst I recorded this 😂 So anyway, I added the following amendments to the above base mix of 4.5 gallons; ♡ 3 TBSP Ecothrive Life Cycle ♡ 3 TBSP Vitalink Bat Guano ♡ 3 TBSP Ecothrive Charge ♡ 1 TBSP RHS Mycorrhizal Fungi granules ♡ 6 TBSP Ground Cinnamon.

I'm running a little test here🤓, I have cut the upper tops of the plant, and left the buds that were getting almost no light. I'm curious whats gonna happen... The idea is off course that those bud will turn out thick and fat as well😋. I'm gonna keep a close eye on the trichomes, so nothing gets out of hand and turns to brown. The shock of the harvest will take at least a day or 4 for them to get back I think, but maybe even a week... After a week I'm gonna decide if I will chop, or give m another shot overdrive. It all depends on how the trichomes look and the overall look of the plants.🤓 Have a nice week and till the next🤙🏼

Then we start flowering. 💚

Hi all the happy people here in GrowDiaries. This is my second cultivation ever and it will be fun to try a bigger space than my closet grow. First, I'm just going to say I'm done with the construction of my new growroom. I put some pictures on the construction here in week one. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable theme in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 8 pcs 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. But there are no cultivation rooms to be displayed here, so I continue with what is most important. Today I have put my seeds in my moisture dome and hope the seeds have germinated within a few days. I am very excited to see how the new growroom will work and how this Blue cheese Auto from Royal queen seeds will turn out. Blue Cheese automatic cannabis seeds are a cross of Blueberry Automatic and Cheese Automatic that has been perfected over five years of breeding and careful selection. The result is a feminized, autoflowering strain with superior yield, flavor, and effects to either of its distinguished parent strains. THC: 16% CBD: Low Yield Indoor : 325 - 375 gr/m2 Yield Outdoor: 60 - 110 gr/plant Height Indoor: 40 - 70 cm Height Outdoor: 60 - 110 cm Flowering: 7 - 8 weeks Harvest month: 9-10 weeks after planting Genetic Background: Blueberry x Cheese x Ruderalis Type: Sa 35% In 40% Ru 25% Effect: Cerebral and uplifting Climate: Mild .............................................................................................................................................................................................................................................................................................................................................. Update 2017-08-15. All 4 seeds have germinated and planted in small pots inside the humidity dome. I'm so glad it worked so well and now it's just hoping they'll start growing and become 4 big healthy girls :) ............................................................................................................................................................................................................................................................................................................................................... Update 2017-08-16. I have mixed my own soil today. Its 40% sieved peat harrow H2-H4 0-30 mm, 45% sieved peat harrow H4-H6 0-30 mm. 5% sand and 10% of compost soil. And i use 15% of perilite and mix it all together. ................................................................................................................................................................................................................................................................................................................................................ Update 2017-08.20. Nr1 and 2 grow and thrive. Number 3 just got rid of the seedpod and is folding out the sheets. Number 4 is a bit shy and can only be seen if you look carefully into the soil. ......................................................................................................................................................................................................................................................................................................................................... Update 2017-08-21. Nr 1 and 2 is doing well. Nr3 is trying to catch up and Nr4 is still shy. -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-23. Transplanted 2 girls from humidity dome and the small germinating pots to 15 liter autopots. The other 2 girls have to wait a little more before transplant to the autopots. ................................................................................................................................................................................................................................................................................................................................................................................ 2017-08-27. The girls have settled in to there new home of the 15 liters autopot after a little transplant chock. Now the real week 1 starts for me and the girls. -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-28. New pic and movies. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-28. Nr3 is transplanted in 15 liter autopot. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-29. New movie of the girls. Its no water for 3 days now for Nr1 and 2 so the roots develop more and match the plant above the soil. The temp controlled fan is awesome, its easy to set what temp you like to have in the room. Right now its 28 celcius. And humidity is 56%. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 2017-08-29. Hello to you who read my diary, I just want to say that I am pleased that you have chosen to check in with me and in my diary. I just want to say that I do this for myself and for a steady flow of my medicine. Everything you read and see in my diary is 100% honest and I will never distort or beautify anything here. I document my crops so that I can learn from my mistakes and also to look back at those different crops. I try to update with pictures every day and with text if something special has happened in the garden. This is my strainhunt for the best medicine and the beginning of my journey with cannabis and the cultivation of it. ----------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-30. Cleaned the room this morning, just vacuuming and cleaning with chlorine solution. Im testing the fan to control temp and humidity, it works great. Added some pics with measurements and movie. Everything is looking great right now. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-31. New pics and videos. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-09-01. New pics and videos. Gave each of the girls with 2 ml Alg-A-Mic, 4ml Bio-Grow, 4ml Bio-Heaven and 10 ml Formulex mixed in 2 liters of water. I hope they like it :) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-09-02. New pics and the girls liked the water and nutrients yesterday. ------------------------------------------------------------------------------------------------------------- 2017-09-03. New videos of the girls this morning. Nr1 and 3 looks great and nr 2 is looking a little funny, maby a little stressed. Kl. 16.00. Did some defoliation on the girls. Added video. Kl.. 23.00. New videos, defoliation and leaftucking. -----------------------------------------------------------------------------------------------------------------

Everything looks great...going to transplant next week!!!

Hey now, hope everyone is haing a great weekend. The Sundae Driver is doing pretty well, she is 2 weeks from getting wet under the Spectrum X which is only cranked up to 30% so she is putting out close to 230 Watts for the 7 seedlings. I will only keep 5 of them and give a couple away as I think even 5 mainlines will be tight in my 5X5 tent. My plan is to transplant them in a couple days, and then top them for the first time at the third node around day 20, and then hopefully top her again around day 27, then again hopefully around day 30, and then flip her to flower by then end of week 6 or 7 at the latest. If i go much past there I think I will run out of room but we will see how it goes. First time ever running a Royal Queen Seeds Strain, but I have smoked this Sundae Driver before and she was one of the tastiest strains I have ever tried, so very excited for this run. I hope everyone has a great weekend, thanks for stopping by, Stay Safe and Blaze On!!! 💪 Website: https://medicgrow.com/ https://growdiaries.com/grower/medicgrowled

Que pasa familia, vamos con la segunda semana de crecimiento de estas FBA2506 de FastBuds. Las 5 semillas una vez germinadas son plantadas directamente en maceta de 5,5l La tierra que utilizamos que está en la publicación anterior es top crop all mix, Por supuesto el ph se mide en cada riego y se mantiene en 6.2, regando cada 48 horas e intentando mantener la humedad un poco alta al principio. Las próximas semanas vamos viendo cómo avanzan. Hasta aquí todo, Buenos humos 💨💨💨

Day 1 Week 10. Added AN Big Bud to the feeding as per Advanced Nutrients feeding calculator. Still fighting to keep the stretch under control. And the RH is hitting 80% leaving the door partially unzipped. Ordered a small Dehumidifier to help. Changed the cover picture in celebration of an election. Day 3 week 10. Added a dehumidifier to help bring the RH inline. Sofia and Claudia are starting to show their flowers. Happy girls make happy grower. Day 5 week 10. Each morning a defoliation, and the next day grown in again. The girls have grown enough to see where they are joined from their single seed.

Beautiful purple buds there are only 2 out of 5 phenos of bslck cherry punch 🍒 👊 that have purple flowers. The aroma just like her sisters is ver very sweet for real. Very nice fruity sroma, it gives you very good vibes man. I'm in love with this strain, basically because the 5 bslck cherry punch have the exact same aroma, and that's awesome because obviously these are not clones as you can see,all of them started from seed, and I think I've found a very good híbrid to my favorite list. She's been grown 100% organically without any sinthetic nutrients, no bottle nutrients. Just 100% organic living soil with very rich in beneficial bacteria and fungus thanks to the use of Florganics FLO, Silicium flash by biotabs that contains bug shit very rich in life. And also bat guano and seaweed powder by guanokalong. Peace everybody! 💚✌️❤️👨‍🌾

Gracias al equipo de Anesia Seeds, XpertNutrients y MarshHydro, sin ellos esto no seria posible. 💐 🍁: Dankberry es nuestra nueva variedad premium con la inconfundible fuerza y singularidad de Anesia Seeds. Esta variedad de cannabis te ofrece un subidón excepcionalmente fuerte e impresionante y un delicioso sabor a bayas maduras y nueces - igual que un desierto extraordinario. Tras mucho tiempo de duro trabajo de cría, presentamos con orgullo esta variedad feminizada que volverá a establecer nuevos estándares en el mercado del cannabis. Se originó a partir de un cruce y selección de Little Devil y Skunkberry, dos cepas únicas que establecieron puntos de referencia para la producción de cannabinoides. Dankberry tiene 2 fenotipos ligeramente diferentes en cuanto a crecimiento y tiempo de floración. El tiempo de floración de la variante más achaparrada y de crecimiento más oscuro es de unos 60 días, mientras que el fenotipo más brillante y de crecimiento más alto necesita entre 5 y 10 días más. Alcanza cosechas por encima de la media de hasta 550g por m2 en interior. 🌻 🚀 Consigue aquí tus semillas: https://anesiaseeds.com/product/dankberry/ 💡 Mars Hydro TS 3000, como la lámpara de cultivo LED más grande de la serie TS, ofrece suficiente cobertura para un área de 4 × 4 pies con un precio asequible y rendimientos de calidad; a cambio, se puede aplicar tanto al cultivo doméstico como al cultivo comercial. Potencia - 450w Cobertura Vegetal – 5×5 pies Cobertura de flores - 4 × 4 pies La opción abrumadora para la mayoría de los productores que la aplican en tiendas de campaña. Consigue aqui tu lámpara: https://marshydro.eu/products/mars-hydro-ts-3000-led-grow-light/ 📆 Semana 7: Última semana de crecimiento, esta semana pasada se han mudado a la nueva carpa de Marshydro 1.50x1.50x1.80 y necesito algo mas de tiempo para cubrir el espacio vacio. Practico poda apical para equilibrar el tamaño con las mas pequeñas. Aplicación de jabón potasico via foliar para prevenir plagas. Aplico 1/4 de la dosis de nutrientes recomendada por el fabricante, todo va bien.

Estamos con el lavado de raíces y esperando al ámbar. Hubo unos días de mucho viento pero aguantaron perfectamente gracias a la red de scrog.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Un altra settimana è passata e tutto procede bene.per fortuna non ho più trovato nessun nanners 🙏✌️💪..belle piantine mie, continuate così che farete delle belle pannocchie.... 30/8/24 gente ho combinato una bella cazzata.pur avendo esperienza, mannaggia a me,mi sono portato a casa dentro il box, il cazzo di ragnetto rosso... fanculo... Ho già iniziato a ripulire tutto, box e piante con Neem...li sterminerò tutti 😂..