So we have harvested the wedding cheesecake abruptly as I think one might have started too herm possibly as I saw signs hence the abrupt chop of all as only days away from end of week 8. There are no seeds or any disinformation yet I saw under scope slight growing signs of possible coming g of herm so didnt waste time too chop... will inspect once dry all should be okay 🤞 The buds smell absolutely delicious theres more of a creamy cakey aroma bursting through more than anything like a creamy woft through the air. The girls grew amazingly considering I went from auto feed too hand feed although didnt seem too have a negative affect end result shows so I'd like too re run the wedding cheesecake ff again solely by hand/autopot this time all way through. Buds form grameat with great resilience too conditions out of control as I myself have a heatwave and they seemed too handle well. Over all I would strongly recommend this monster of a grower too all, she is a beautifull strain with such a great growing process. I will keep one girl for flower and the not so perfect girl for hash or too be pressed once cured for a month or so... These were flushed only a week unfortunatley due too early chop but as in coco I'm sure I'll be fine after a cure even for the hash girls.

Remarque : nutriments à 50 % de la dose recommandée. Peu d'évolution, se développe très lentement.

Day 29 Mon Refresh water and add nuts 10 L PH 5.8 EC 1.1 DLI /13h/ PPFD/ Water 20c Day 30 Thu PH 6.5/5.8 EC 1.1 DLI /13h/ PPFD/ Water 20c Day 31 Wed Add water PH 6.5/5.8 EC 1.3/0.9 DLI /13h/ PPFD/ Water 19c Day 33 Fri PH 5.9 EC 0.9 DLI /13h/ PPFD/ Water 21c Day 34 Sat Add water PH 5.5/5.7 EC 1.0/0.9 DLI /13h/ PPFD/ Water 18c Day 35 Sun Add soda bicarbonate for Ph-up PH 4.8/5.8 EC 0.9 DLI /13h/ PPFD/ Water 18c

Light up to 70 %power. Run off was 1200ppm organic still so will feed next time for first time bloom nutes. White preflowers starting to shoot up all over the place. Will continue with a bit more lst throughout the stretch period.

good day :) here she is, the long-awaited girl cookies:) in another week, she will travel to a 40x40x120 cm growing box, and 50 w light:) it will be interesting to travel with her :)

2025-10-02 And again a weather change- to clear weather and some sun- thats fine in the mornings some fog- and then sun until evening and quite cold nihgts perfect see you next week

Had a ph problem last week as my pen decided to out of the blue go wonky. Made my plants basically drown till I caught it but these girls actually didn’t take it to bad. Only got a bit of leaf damage and plants seemed to bounce back overnight. 2 of 4 are tied down now and the others will be done this week as well. I will also be installing my Scrog screens at the end of the week.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Start of week 6, day 45, 7th oct. All topped, and have had a problem with little caterpillars, switch to flower soon, so far so good.😜

This week I am still working on my slow defoliation, to get some light intake to some of the node sites, while putting the plant under as little stress as possible. I've still been using the molasses, Mykos WP and Fish Sh!t mix every other watering. I also top dressed another 1/4 teaspoon of BioAg TM-7, which once again seemed to help both plants keep their leaves held up longer between waterings. I assume the TM-7 is improving the water retention of the Build A Soil Lite I'm using. I still have the Viparspectra 100w boards, set to about 30% power kept about 14 inches away, this seems to be a good spot for the plants. I'll probably be taking the bottom branches off and cloning them this week, so I can back up these genetics. Update: I went ahead and cloned these ladies to back them up, they are my first plants from seed, so I wanna keep the genetics if I like the flower! I used clone-x, root riot cubes, my AC infinity Germination Kit with 80w led bar on setting 2. I sprayed the clones and inside of the kit with distilled water. Hoping I get some clones rooted soon!

I have a hard time adjusting the room temperature because of the air conditioner failure. I started growing with one plant, a week later I added two more, and the next week 2 more.This is why my job is really hard. AK 49s have not started blooming even though they are in their 6th week!DNA genetic seeds and I bought them 3 years ago. In my previous cultivations they flowered in 4 or 5 weeks.With the 12/12 light cycle I encourage them to bloom.Since they're all at 6 weeks of auto seeds, I've started adding the bloom fertilizers. I guess I just need a little luck and good wishes! Please send your beautiful energies...Thanks :))

Day 14

The girls have chilled right out growing up and are growing out an phat , I’m seeing some purple there . Big defoliation coming up as soon as I can move that auto out which is looking chunky as but a bit on the small side prolly due to the 12/12 , still odds on to have some fresh Chrissy smoko .

420 Fastbuds FBT2304 Week 7 Hello growmies, I hope everyone's having a good weekend. Week 7 update for these two. One is definitely taller than the other but overall structure seems to be pretty close. Colors are good and starting to smell. Still keeping up the same feeding schedule of notes every 3rd water and just ph'd water and calmag for the other 2. Seems to be working great. All in all Happy Growing

2 Pheno still in veg living da solo cup life

Week 02 Introduction This week has been a bit of a struggle. Had my first experience dealing with slime and brown algae. But I think adding enzymes and increasing the beneficial bacteria helped to fix the issue. Also experienced nutrient burn for the first time. My nutrient solution strength got as high as 671 PPMs. So backed it down to about 430 PPMs during the end-of-week reservoir change. Also cut Hydroguard (expired?), CaliMagic, and Superthrive from this grow. Now she's still stunted, but is starting to recover. Hopefully... [START OF WEEK 02] Day 01 - Monday - 08/10/20 - Total Days: 008 ----------------------------------- - [0830]: Still looks a bit yellow. But getting larger! --- Solution strength was at 248 PPMs. --- Just topped off with a bit of distilled water --- PH went up to 6.1 so PH'd down to 5.8 --- Ran the top feed for a bit to wet the hydroton. - [2130]: She's looking even more yellow now. --- Even the new leaves are really yellow. --- Decided to add more base nutes. --- Added 4ml of Sensi A --- Added 4ml of Sensi B --- Now at about --- Solution Strength went up to 304 PPM. --- PH was at 6, --- Also raised the water level a bit more. --- I really hope it's a nutrient deficiency and not an over-watering issue. --- Please live! Day 02 - Tuesday - 08/11/20 - Total Days: 009 ----------------------------------- - [0830]: Looking less droopy. But still yellow. --- Decided to do a reservoir change. --- 4.5gal of distilled water --- 8ml of Sensi Grow A --- 8ml of Sensi Grow B --- 8ml of Voodoo Juice --- 4ml of B-52 --- 4ml of CaliMagic --- 4ml of Hydroguard --- 4 drops of Superthrive --- PH'd down to 5.8 --- Solution strength: 302 PPM --- Since I didn't want to risk over-watering the rockwool, I did not to do a top feed, may do one tonight after the sleep cycle (lights are still set to go off at 10am and back on at 4pm). --- As I was doing a reservoir change, I noticed that the tubes and bucket were a bit slimy. Didn't really stink or anything. Is this normal? Maybe due to the Hydroguard and Voodoo Juice? --- I hope she gets better soon! - [2215]: Getting larger! But still yellow. --- Also can see a root growing through the net pot! Tiny little thing but it's there! Will have to start dropping water once more appear. --- After looking over a few DWC diaries using AN nutes, seems like my solution strength is still too low. Most around this stage average around 500 PPMs with about 4ml per gallon. --- Top fed for a minute to wet the top of the hydroton. --- Solution strength went down to 263 PPM. --- Added 8ml more of Sensi A --- Added 8ml more of Sensi B --- Now base nutes are at 16ml for 4.5 gal of water. --- PPMs went up to 411. --- Still lower than 500 PPMs, but that's okay. --- PH was at 6. Not sure if I should adjust PH using AN nutes. So going to not ajdust unless PH goes above 6.1. Day 03 - Wednesday - 08/12/20 - Total Days: 010 ----------------------------------- - [0830]: Looks like more root growth! --- She still looks yellow. But leaves are perky. --- PH was at 6.2 so lowered down to 5.8. --- Solution strength was at 394 PPMs --- Since roots are still growing, I decide to lower the water level a bit. Now water level is a bit below the net pot. - [2200]: Root is getting longer! --- Slime is returning. Not sure why. --- Solution strength: 357 PPMs --- PH: 6.1 --- Decided to add an enzyme along with more beneficial bacteria to hopefully cure the slime. --- Added 16ml of Sensizym --- Added 5ml of Hydroguard --- Added 8ml of Voodoo Juice --- New solution strength: 430 PPMs --- New PH: 6.1 --- Top fed for a minute. --- This slimy residue is really freaking me out! I hope the added enzymes will help eliminate the slime. --- Going to do a full reservoir sterilization/nutrient change over the weekend. Please survive until then girl! Day 04 - Thursday - 08/13/20 - Total Days: 011 ----------------------------------- - [0930]: I think the enzymes worked! There is little to no slime now! --- Although I think the root was also dissolved in the process. It's now just a little stub sticking just below the net pot. The enzyme is also suppose to dissolved dead roots too but guess that root was too weak? Not sure what happened there. But I think the slime is cured! --- Solution strength went down to 395 PPMs. Decided to add more base nutrients. --- Added 8ml of Sensi A --- Added 8ml of Sensi B --- Added 4ml of B-52 --- Solution strength went up to 598 PPMs. --- PH was at 6.1 --- As long as the PH is between 5.5 and 6.3, I'm not going to use PH Up or Down. But still will monitor PH levels. - [1715]: Checked up on her after her nap and shes looking alive but stunted. But no slime! --- Decided to add some sugars to help maintain the enzymes and beneficial bacteria. --- Solution strength was at 587 PPMs. --- PH was at 6.2 --- Added 8ml of Bud Candy --- Solution strength went up to 671 PPMs. --- PH also went up to 6.3 --- Lowered water level a bit to hopefully promote more root growth. --- Top fed for a minute. --- She is getting bigger, but still looks yellow. Day 05 - Friday - 08/14/20 - Total Days: 012 ----------------------------------- - [0915]: Leaves look a little burnt on the tips. Still no slime. --- Going to back off on the PPMs to hopefully fix the nutrient toxicity. --- Solution strength was at 632 PPMs --- PH was at 6.3 --- Added/Removed water until PPMs were at 536. --- PH was still at 6.3 --- Raised water up a bit, then top fed for a minute. - [2200]: Looking better. Still yellow though. --- Solution strength: 514 PPMs --- PH: 6.5 --- Added PH down. --- New solution strength: 518 PPMs --- New PH: 5.2 --- PH was kind of high. So decided to PH down. Had to use quite a bit to bring it down. But eventually got it to drop down to 5.3. A little low, but so far, the PH has always gone up so wanted to start it really low. --- Ran the top feed for a minute to re-saturate the top of the hydroton. Day 06 - Saturday - 08/15/20 - Total Days: 013 ----------------------------------- - [0830]: She looks stunted. leaves are still yellow. --- Solution strength: 514 PPMs --- PH: 5.6 --- Top fed for a minute. --- Not sure what to do at this point. Going to do another reservoir change soon. Hoping she lives until then. - [2145]: Looking pretty much the same. --- A bit of slime has returned. Since I've been diluting the nutrient solution, the enzyme ratio has dropped. So I guess this is expected. --- Also noticed that slime build up was on the air-stones. Never really checked the stones so not sure how long that has been there. Looks brown too, which can't be good. --- Going to do an H202 sterilization of the system (minus the net pot) tomorrow so did not add anything. --- Lowered the water level to below the net pot. --- Then top fed for a minute. Day 07 - Sunday - 08/16/20 - Total Days: 014 ----------------------------------- - [0900]: STERILIZATION DAY! --- Decided to skip today's night cycle for a deep clean. --- I'm hoping that skipping a night cycle won't be too stressful. --- Temporarily moved her to a new bucket. --- Used about a half of gallon of straight distilled water to flush out the net pot. --- Drained the system and wiped up all the brown sludge. Also removed the air stones and cleaned up all the goo. --- Filled the system with 2 gallons of distilled water and at least 30ml of food grade hydrogen peroxide (35% strength). --- Yea its a lot, but since I'm not going to sterilize the system overnight, I went with a really high doseage. Basically I kept adding until I started to see little bubbles start to form. --- Then I ran the water pump. - [0915]: Boiled the air stones in tap water for about 10 min. Then reconnected them to the airlines. --- I let the air stones and hose soak in the sterilization solution for about 5 minutes so the peroxide can absorb into the stones. Then ran the air stone to help mix and agitate the solution. --- Occasionally, I'd repeat the process of turning the air pump off for a few minutes to let the stones soak. - [1000]: Covered the top two row of holes of the net pot with some aluminum tape. Hopefully this solves the light leaking in through the hydroton. - [1200]: Used an old toothbrush and scrubbed the pump clean. Then drained the system. --- Refilled with more distilled water and peroxide. Again added a lot. --- Ran system again to sterilize even more. - [1530]: Drained the system and refilled with distilled water to rinse. --- Ran the system for a few minutes, then drained and rinsed again. --- Did this a few more times over about an hour. - [1630]: Added 4 gallons of fresh distilled water. --- Added 15ml Sensi Grow A --- Added 15ml Sensi Grow B --- Added 12ml of B-52 --- Added 16ml of Voodoo Juice --- Added 16ml Sensizym --- Solution strength was at 430 PPMs --- PH was at 5.6 --- I was going to add Hydroguard, but I think the bottle is expired. Since 2017 or 2018!? I think Hydroguard is good for up to 2 years from the date on the bottle or 6 months after opening. So not going to use from now on. I wonder if this could have contributed to the slime build up. --- Also removed the CaliMagic and Superthrive too. Going to only use AN products to eliminate the chance of incompatible nutrients. --- Placed her back in the bucket and top fed for a minute. --- Here's hoping for the best. Going to let it ride until morning! Please feel better! [END OF WEEK 02]