3. März Mal wieder Montag: Es beginnt Woche 7 für die Orions bzw. Woche 6 für die kleine Crit. Sieht alles im allgemeinen sehr zufriedenstellend aus. Die Fadenbewässerung war bereits wieder halb leer. Die Töpfe waren allerdings gefühlt sehr leicht und nicht so schwer wie sonst. Habe das Gefühl dass die Bewässerung seit dem letzten Mal befüllen nicht richtig gearbeitet hat. Dennoch ging das Wasser schnell leer. Ich gehe nicht davon aus dass es einfach nur verdampft ist, da die Luftfeuchtigkeit sich im Rahmen gehalten hat. Keine Ahnung… Habe die Fadenbewässerung erneut befüllt und dieses Mal die Fäden explizit nass gemacht. Zusätzlich gab es 1L Wasser+ 10ml Bio P-K 5-8 für jede Pflanze von oben gegossen + 0,5l Wasser nachträglich pro Pflanze zum „nachspülen“. Das restliche Wasser der Woche wird nun hoffentlich wieder von unten durch die Fäden bezogen. Dieser blöde rotklee geht mir so unglaublich auf den Geist!!! Ich hab’s zwar schonmal protokolliert aber doppelt hält besser: nie wieder soviele Samen aufeinander pro Topf benutzen!! Vor allem bei der Crit ist es sehr schwer von oben zu gießen weil alles voll mit dem kack Klee is. wuuuussssaaaaa

I did alot of fuck ups so ended up with a small yeild but ended up having really dense nugz the smell of her is amazing sour apples smell with the taste being musky with grapefruit and cheese taste

11.07. Rainbow Sherbet 1# Day 77# Rainbow Sherbet 2#Day 75# On rs2#, during the storms, one branch at the very bottom broke, it grew like this, I didn't want to tighten it, it will probably break already during the next storms, but nothing serious. Today is the end of the eleventh week for the plants. There were storms all week and with the fact that they spent almost the whole week under stress (they were also under heat stress a couple of days before), I am certainly satisfied with the progress. On Tuesday, they were watered for the last time, with clean water, then it rained, and the next day it rained all day, since then they have not been watered, as soon as the soil dries I will feed them. Below in the table is the food that they received last time, but it is included in this week, and I introduced that as well. Stay High and Keep Growing!!!

This little lady has grown so fast I haven’t even had the chance to take pictures. All try my best to keep up on her. Have her a nice bend or low stress training see how she goes.

Tropicana had to go back indoor because the temperature outside is the opposite of tropical. I took off two leafs and gave her time to relax after. Everything is fine and I can already smell something when I‘m near to her. 🤭☺️ Where is the sun? Preparing for sun storm!

She is pumping out so good now. When I opened the tent before I was blown away with her scent. A very pungent sweet citrus smell with hints of berry. Will be doing another flush tomorrow. And will be preparing for the harvest. Am absolutely over the moon with her. I see where I can make improvements for a better yeild next time but the quality of her is outstanding by the eyes and nose.

Just starting to see the autos flower today, so the stretch is on. All topped and lst except for 2. Photo veg is on, waiting on some clones to root. Room will be filled with 3 top cultivars: 1) Pink Sugar Breath by Visions (GD growmies, top cross, check him) 2) cactus breath by Barrie Grower. Amazing cut 3) Slurricane by Inhouse. If you know you know Special appearance in a scrog net made by Peanut Butter OGKB gifted to me by Original Sensible Seeds. These are one of the very few breeders here on GD that I will sprout. Amazing every time Hope everyone has a good week, except you bots. Bots go fuck yourself

Super schöner Grow :) Etwas unterschiedliche Farben, wahrscheinlich wegen unterschiedlichen Lichtanteilen in den LEDs

Excelente experiencia, volvería a cultivarla nuevamente.

Die Ladys entwickeln sich Planmäßig,auch die Ersatz Pflanze gibt jetzt gas . Nachdem die Ladys so extrem Buschig untenrum wurden hab ich sie ein wenig Entlaubt.(Lollipoping)

Week 7 there hasn’t been any changes for either of the girls. Girl #1 is still looking the same. It seems she has finally stopped stretching. Looking forward to seeing her bulk up in the coming weeks. Girl #2 still hasn’t showed any signs of flowering. I’m gonna continue to be patient with her in hopes of her just being a very late bloomer. Everything has been running smoothly. I will start to introduce bloom nutes in the next coming weeks. I didn’t expect girl#1 to get so big but I’m not complaining. This has been such a pleasing and educational experience. Thank all of those who have viewed my diary. Thank you again for your support.

Week 2 and nice and green, we just gave some more organic nutrients like palm tree ash and some guanokalong powder, for the moment its seems to be all good, in 2 weeks I'll be adding some sugars and some more PK

Week 9 for Kalini Asia by Zamnesia seeds, She really bounced back from her last topping to 16 tops. now just keeping her levelled in height. Because of the messed up light schedule + massive shifts of temps from 28+ to 6... outside the tent so really for 7 hours their growth is completely stunted, but I'm making due with what I have 😂

Vegetation Week 3: Steady Gains, Environmental Harmony & the Art of Boring Update: 30.03.2025 What’s up, my Growmies? Week 3 with the Blueberry and Durban Poison was blissfully… ordinary. No drama, no breakthroughs—just the quiet hum of plants doing their thing. Here’s the low-key recap! Current Observations: Subtle Wins Leaf Development: The first true fan leaves are finally unfurling—wider, darker green, and more serrated. My Blueberry lady is slightly ahead of my Durban Poison babe! Stem Thickness: Those once-spindly stems now feel sturdy and stable, thanks to their exposure to light winds. Environment Report: Stability Wins The weather’s been a steady 10–16°C with occasional clouds. Plants are now outdoors soaking up direct light as long as possible (still bringing them in at night—better safe than sorry with these chilly temps!). The "Boring" Philosophy No news is good news! This week’s mantra: Don’t fix what isn’t broken. Watering Rhythm: Sticking to plain pH’d water (~6.3) only when the pots feel light. No overthinking, no overwatering. No Nutes Yet: Biobizz Light Mix is still feeding them plenty. No rush to disrupt the balance. Future Prep: Laying Groundwork Topping Plans: I’m planning to top them next week for the first time! I’ll decide spontaneously if the girls look stable enough, but I’m leaning toward waiting until Node 5 to minimize stress. Slow and steady wins the race—no stunting allowed! Week 3 Takeaway: Slow, stable growth means the plants are content. Sometimes, boring is beautiful. Until next time: Stay grounded, stay growing… and stay blazing! – Smoking_Joe_Frazier

noto que estan comiendo mucho, osea se secan rápido que es que estan comiendo bastante de lo comun. Las ojas tornandose color amarillo para cambiar a color vino. se ven muy bonitas y estan fewlices. No he cambiado mucho entre los nutrientes (Té y Recharge), he sostenido alimentarlas simples y he tenido muy buenos resultados. muy contento con todo, sigas adelante, buenos humos y feliz 2021

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Harvest time for Mandarin dreams round 2. Both phenos did amazing during round 2 under the Mars-Hydro TSW2000 light. The buds are incredible and the way these ladies grew was very impressive. Hopefully they are heavy buds, will know in 7-10 days if they have the dry weight to back up their awesome appearance. April 16 update - MD2 - 70 grams, MD1 - 73 grams. Again results are close to round 1. The TSW2000 light delivers!

HELLO FARMERS , ITS YOUR FRIENDLY BARN RAT BONEY ! Glad to be back for another week, not too much different with my watering techniques. Just getting into some training techniques and defoliation. Been messing around with 432 Hz half an hour twice a day .