Hi everyone ! everything is going well, except i have burn some leafs with light ( i was too greedy and put the light too close ) but it's only on 2 or 3 leafs so it's ok. i can't wait !! i wanna taste it 😍😍😍

Fotos con 6 semanas y 2 días a 12 horas, esto son 44 días, la primera ya murió y durante la siguiente semana han ido muriendo, a 28/5 quedan 3 que dejaré hacerse un poco mas. El olor en general de las que corté era mejor de lo habitual, había mas plantas con olor fuerte que otras veces.

Harvest week coming up. Plant looks really good and Colas have gotten really heavy

So excited that this grow went well. Just making sure the dry tent stays the right conditions for a slow dry and will trim and weigh these girls before putting them in jars for a few weeks to cure. Sticky and with a earthy fruity berry smell. Maybe a hint of citrus. Can't say enough good things. Happy to luck out with good genetics and optimal conditions during the grow cycle.

The bud's are not like I wanted it because the room temperature more than 34 degrees 4 weeks ago I think 2 0r 3 weeks flowering to harvest

update after cure

OG 4Q24 Flower Week 9 Orangegasm (Fem) [ IRIE Genetics ] 12/12 @ Bolt (Day 21) Germination: 20 November 2024 #3A Earliest Harvest Date: 9 February 2025 #3B Latest Harvest Date: 19 February 2025 _________________________________________ Start of Week: [Wed Feb 5, 2024 CR2 4Q24 57:F:8:1] End of Week: [Tue Feb 11, 2024 CR2 4Q24 63:F:8:7] OrangeGasm Fertigation: - MAX: EC: [ 2.4, mS] #Ramping Down - LightIntensity MAX: [ 850, µMol/m2/s] ______________________________________ OBJECTIVES - Ramp EC from 2.4 - 1.0 - Start rolling off Primer A&B/Silica Skin to Resin Bloom . . . __ Wed Feb 5, 2025 OG 4Q24 57:F:8:1 EC: 2.3 Notes: #5 looks like a 60 day plant, if so - consider Saturday Harvest … Runoff - [x] Amount: [ 0.125 , gal] - [x] EC: [ 4.4 , mS/cm] - [x] EC∆: [ 2.5 , mS/cm] # Fried Yet? ;-} Harvest Dehu - [x] Amount: [ 1.5 , g] __ Thu Feb 6, 2025 OG 4Q24 58:F:8:2 EC: 2.3 Refresh Reservoir - [x] Amount: [ 2 , g] - [x] EC: [ 2.3, mS/cm] - [x] Primer A&B: [ 31, ml] - [x] Silica Skin: [ 15 , ml] __ Fri Feb 7, 2025 OG 4Q24 59:F:8:3 EC: 2.3 __ Sat Feb 8, 2025 OG 4Q24 60:F:8:4 - [x] Disassemble, Clean Reservoir and Pumps, Reassemble Refresh Reservoir - [ ] Silica Skin: [ 15 , ml] - Dropping Silica Skin due to (sparse to medium) granular precipitate - [x] Amount: [ 6 , g] - [x] EC: [ 2.1, mS/cm] - [x] Primer A&B: [ 100, ml] EC: 2.1 @1900 - Clear Manifold Filter. (Mainline should be cleared on 1845 feed __ Sun Feb 9, 2025 OG 4Q24 61:F:8:5 - [x] Dilute to EC 2.0 - Flooded Tent, 2 Gallons PLUS of Runoff. Happy Plants. - Shout Out Shop-vac. ;-} EC: 2.0 __ Mon Feb 10, 2025 OG 4Q24 62:F:8:6 EC: 1.8 HAND APPLICATION Prepped One Gallon of 12-8-7-5 @ 1.8 mS/cm w/ PhotoSynthesis Plus, SLF-100 Reclaimed Dehu Water, ~2 Gallons Refreshed Reservoir, 2 Gallons Rooted Leaf Hydro no Silica Skin, EC: 1.8 mS/cm Reduced watering to 5 min, then 1 - 1 -1 -1 -1 -1 -1 -1 (It’s not like they’ve been under-fertigated) __ Tue Feb 11, 2025 OG 4Q24 63:F:8:7 EC: 1.7 - [x] EC: [ 1.7, mS/cm2] - [x] Refresh Reservoir - [x] amount: [ 2.5, gal] Runoff - [x] Amount: [ 0.5, gal] - [x] EC: [ 3.6, mS/cm2] - [x] EC∆: [ 0.9, mS/cm2] # Better than 2.5! PLANT POSITIONS 3 5 1 7 4 2 6 8

It’s getting frosty as in the tent , nice colour and beautiful smell coming from the chunky bud , she’s a slow mover and still has a few weeks to go but no complaints here it’s gonna be a bumper yield of some great weed

Going smoothly, got a 3rd Trellis in case they grow to high

Day 101, been cutting off fan leaves on lower nodes, adding ice cubes and spraying cold water on buds. Expecting about 5 more weeks till harvest but I don’t know just guessing Also I don’t know how to change the question so I’m gonna put it here, has anyone tried to defoliate some smaller colas then tie them together will that be beneficial for bigger bud(promote one another) or is it better to leave oxygen to pass through, thanks

Semana 5 dia 35 . La planta se desarrolla de forma claramente índica. Como se puede ver en las imágenes la poda apical ha generado que de nuevo vuelvan a brotar hojas deformes aunque la salud general de la planta no se ha resentido. Esta será la última semana de vegetativo, deberían haber sido cuatro, pero la primera semana no se desarrolló correctamente. He colocado la malla scrog para ir guiando las colas para sacar idealmente 19 colas. También he cortado dos hojas grandes para favorecer la penetracióin de luz y volveré a hacerlo probablemente en la semana 7 o finales de la 6 para favorecer la ramificación dia 36 - He cambiado el ciclo de luz a 12-12 con la malla bastante poblada, también he cambiado la dosis de abono que añadiré en la siguiente semana.

Finishing out week 5 of flower and continuing my daily treatment for PM. Using Athena Mildew Control, an organic reactive treatment spray that you can use on the entire plant up to harvest. Realizing PM is systemic and is like herpes...it never goes away! All you can do is spot treat on a daily basis, the outward symptom of PM, which is the white patches/spots. Treating primarily Mimosa, but also parts of the Bling as well. Napali Pink - As usual, is impervious to this type of PM and is thriving, with huge fat buds that still continue to get fatter and tighter by the day. They haven't even fully swollen up yet either. If the smoke is great, which I am sure it will be, this is a strain that I highly recommend growing. It has excellent branching structure with upward growth with very little help. Takes nutrients well and not too fussy or needy with any of variations of NPK or micros. I would say based on the looks of the buds and trichs, she will be a last to finish, but well worth the wait, as I have so. Many. Fat. Ass. Buds!! Mimosa - such an amazing daytime strain that I would loove to always keep on hand, as this strain is on of my All Time Favorites! However, with such prized buds and terp profile, she is vulnerable to PM. She has had it the worst, and always continue to spot treat it daily. She is gaining this cool hue of purple along the outer edges of the leaves, as well as what I thought was PM, but surprisingly turned out to be a shit ton of trichs! Buds are fattening up nicely and think she will be the 2nd to finish out of the bunch. If I could grow her again, I will, but will be sure and do a preventative treatment for PM during her entire life cycle. The Bling - she has been a trip growing, as she is very viney and lanky, but her branches firm up nice and strong. Practiced some Kushman Chiropractic on some of the stems with success. Had to spot spray, but not as bad as Mimosa's PM. Since I defoliated all leaves covering bud sites, along with huge fans, the buds have actually grown in height and girth! This one never ceases to amaze as she has come back from a bad HST. She is so heavy with trichs, it looks like it snowed on the buds. I am hoping to get a good yield but between the three ladies, she will come in last for that. Used bamboo stakes to secure all branches/bud heads; used zip tie and paracord to secure to bamboo. Now every single bud gets light! Also testing out a Diminishing Light Schedule and now down to 11 hrs of daylight this week. Will continue to reduce by 1/2 hr each week until I hit 9 hrs of daylight. I will hold at that. Otherwise, patiently waiting for harvest!

Dec 26 @8pm/End of day 16: LST Begins and one top leaf defoliated for light penetration Dec 26 @10pm: Turned bloom lights off (cobs) and left veg lights on. Lowered lighting to 12". Giving her a short break from the heat and time to stretch while I LST her. Her humidity levels are up around 54-60% with cobs off and humidifier running. Dec 27 @4:20pm: Turned veg and bloom lights back on and left light at 12". Dec 27 @9:30pm: Watered her with 2.5L at a PH of 6.56, soaking the whole pot through for the first time. PH runoff was 6.79. Defoliated another leaf for light penetration.

Esa familia , estamos otra vez y aquí actualizo la sexta semana de floración de las BlueBerry Cheese del banco de semillas ZambezaSeeds. Estas tienen buen color y el proceso de floración es el correcto su progreso es increíble. Llevo una semana aplicando el ExplotaCogollos de Agrobeta que es Brutal como compacta las flores , palabra. Que ganas ya de acabar estos proyectos y empezar con cosas serias. Os adelanto que lo próximo que que viene son Gelato y Lava Cake, y después Titán F1. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Las maximas de temperatura no superan los 26 grados y las mínimas no bajan 20, así que no me puedo quejar. Los niveles de humedad también son los correctos van entre 50%/65% de humedad relativa. Por supuesto el Ph lo estamos dejando alrededor de 6. Hasta aquí es todo, buenos humos 💨💨💨.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Blue Dream Auto is doing good. She has about 2 weeks left. Everything is looking good. Thank you Spliff Seeds, Medic Grow, and Athena nutrition. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g.

Not much to do this week. Plants are doing great ! Since a few weeks the leaves have small spots. They are not insects, not spider mites. But I don't know what it is. Started to add BioFinale PK 0.5ml/500ml water/24l pot