Showing lots of white hairs on the bud sites it’s alive

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Double Dunked got harvested 16/11 and I'm planning to let her dry for 14-21 days before trimming and put them in glass+boveda (58%) and grove bags for final cure. Drying conditions are as follows: 16-18°C 55-60% RH

Sanitas y creciendo muy bien.

They should be ready in two weeks.

Favorite N Best Structure N Nug Stacking N Density Tropical Aroma Strong Terps Frosted Colors Starting to pop

Being away over the new years I was unable to tend to the ladies. However, we received an update from friend

Alles gedeiht, aber es bahnen sich CalMag Probleme an.. normalerweise habe ich bisher erst in der Blüte auf CalMag zurückgreifen müssen, wahrscheinlich ist die Wachstumsphase mittlerweile aber auch sehr lang und sämtliche Speicher werden aufgebraucht sein. Ich werde vorsichtig damit anfangen, CalMag aufzudüngen. Bin aber relativ zurückhaltend, da ich in der Vergangenheit so oft Salzstress ausgelöst habe

Grown it many times, clear favorite. Sweet baked gas, spicy, hashy, some have a subtle smooth lemon sherbet aftertaste. Relaxing happy and mellow effect. 22-28% Rosin return

Flipped to flower to see what she can do because so many clones took! I'm so super new that it's still so exciting every time I figure something out. Definite pistils at day 3 of flower

Thex "Sensi Seeds" for this grate genetics. She's growing so fast and have really nice structure 😍😍

Here comes the first Preflower Update

Spoted no more herms! decreased UVA to 6h again because some budtips turn white (light bleach) even the lights are 40cm away...last time they bleached too but with light 30cm away... 2 weeks to go

Things are actually looking better 💪🏼🎆🎄🍻 Happy new year to everyone ! Hopefully everyone is healthy and all ur wishes come true.

Day 71 - Starting off week 11 with 2 gallons of water and 15ML of Cal-Mag. She’s definitely starting to beef up and getting pretty frosty and smelly now. Had to remove a few more leaves towards the bottom that we’re dying off and just tucked the rest once she was out back in the tent. Day 72 - Not much happening right now, she’ll definitely be ready for water again tomorrow and tomorrow’s dose will be with nutrients. Just kinda fluffed her up a bit and tucked the leaves before putting her back into the tent. Day 73 - She was dried up again today so she got 2 more gallons of water and nutes. The smell is really starting to get strong, like a sweet funky cheese. Day 74 - Nothing new today, just took her out for new pictures and out her back in the tent and tucked some leaves. Noticing a two branches that just aren’t getting much light so thinking about chopping them off but not sure because it could be too late to do it and don’t want her to be stunted this late into the grow. Day 76 - All looking good now, she was a bit droopy today because I didn’t get a chance to water her yesterday but within an hour of getting water today, she perked right up.

Me encantó la cepa y su facilidad de cultivo ! Aun que tuve muchos problemas ambientales y climáticos . Me atrasé unas 3 a 5 semanas con estas nenas , pero nos recuperamos bien ! Usamos malla scrog chilena #kanovicultivo , lo mejor que he usado en malla scrog , no se le comparan , su espacio preciso! . Las 4 comenzaron en pequeños vasos , luego de 1 a 2 semanas , trasplanté a macetero de 5.8lts , finalizaron 3 en maceteros textil 19lts y 1 en macetero de 20lts AirPot ! Comencé a brindarle 20hrs de luz / 4 hrs oscuridad , a la 3era semana 19hrs de luz y a la 5ta semana bajé hasta el 18 / 6 . Usé un panel QB de 480w regulables , chips marca Samsung de 3500k , chips epistar UV 660nm iR , el mix completo gracias a mi bro MKleds chile ! Se pasó ! De todas maneras recomiendo una potencia mínima de 600w ( QB LED ) para espacio de 120x .. Usé un bio estimulante chileno , que a mi parecer fue uno de los mejores que he visto y usado ! El grande #trichomemania , te cuento que este producto activa un mecanismo de defensa en la planta (SAR) , haciendo que reaccione y se trate de defender con mayor producción de resina , terpenos y tricomas ! Es increíble ! A los 24hrs tienes resultados , un saludo grande a los hermanos ! El mejor producto 10000000%.... Grande eva seed , buenisima esta cepa , me dí cuenta que si se deja madurar un poco más , su sabor es más frutal candy , exquisita cepa !.... Estamos probando con un papel de blunt organico 100% libre de tabaco marca *jamaican buzz*...al fumarla su efecto es notorio , enseguida viene el efecto psicoactivo y cerebral , se viene a la vista un tanto distorsionado el entorno ...la vision un poco más brillante , y a la vez relajado .. Hoy 18 de agosto estamos probando con un papel de celulosa organico marca lion rolling circus ( foto al final del album ) , quema bastante bien , al parecer ya está casi en su totalidad secado , combustión lenta , buen sabor afrutado citrico con toques candy diesel ...uuff efecto despues de 3 quemadas ya por los cielos , totalmente cerebral y estimulante , un gusto charlar con amigos ! Al manipular su fruto , la planta expele un olor afrutado con limon maduro y skunk , con finales de candy terra , una delicia , muy buena cepa , ahora mientras escribo me siento un tanto "mareado" con esa mitad de blunt ! Buenisinaaa!... ____ La verdad cuando comencé a cortar cada planta era como llenar "infinitamente" el armario donde dejaría secandola , se llenó casi la mitad y un poco más sólo con 1 planta cortada ! Increíble producción , yo estimo unos 90g por planta aprox , ya he consumido un buen peso además ...veremos en estos días que esté por completo su secado ! Esta riquísima , sus efectos fuertísimos ...100% ... ___ Una cosecha buenísima, muy parecido como obtengo resultados en comparación con el sodio , todo bien me gustó la experiencia !.... Además tuve la oportunidad de hacer extracción en seco de su materia vegetal . Obtuvimos entre un 18 a 20% de retorno ! Para mi primera vez , geniaaaal ! Su utilizó prensa de 12 toneladas , de apretó a 80°C por 4 minutos . . . Usen lemon king ! Una delicia .

I cannot believe how much growth this week!!! For the first time I've had to higher the light fixture. It is now almost as high as I can put it. I'm kinda regretting not FIMing her now...... I hope she does not grow much more in height!

Just because i realized im not really providing info about my potted grow but I have been using Mother Earth coco plus perlite i added worm castings, bat guano, n vegetable garden 4-4-4. just p.h'd water at 6.50. once in flower i would water with a diluted solution from my dwc and PPM under 200PPM.

Lollipopped the widow x clones since they’re monster cropped clones. Lots of defoliation. All plants are in 12/12; I no longer have a veg tent. I do not have room to allow the plants to continue to veg. After smoking some of the mother widow x plant; I firmly believe it is white widow. The seed I originally planted came from a mixture of berry white and white widow. I wasn’t sure which bud the seed had come from so we labeled it “widow x”.