Dear Growers , Welcome to Week 9 or Flower week 6 // Day 63-70 from Sweet Seeds Pineapple Slush Xl Auto. Incase of Moving to a New Home . I Decided to skip some Weeks from every Diarie of the 12x12 Automatic Project . Everything should be Normal in the next Weeks . Whether you're a beginner or an expert, you are warmly invited to join, ask questions, and share your own experiences along the way! Project Setup & Conditions: • Brand/Manufacturer: Sweet • Tent: 222cmx150cmx150cm • Light: 2x 720 Watt Full Spectrum • Humidity: 50% • Soil: Narcos Organix Mix • Nutrients: Narcos Products • pH Value: 6 If you want Germinitation results like mine , check out Kannabia Seeds with my link [https://www.kannabia.com/de?ref=61966] and grab the germination device or the strains I used . Trust me – it’s worth it for sure ! Get another 20% Discount at all products using the code [GGD] at the Checkout . Stay curious and keep up Growing —we look forward to welcoming you back for the next chapter soon!

Top dress her also… trying out this new fertilizer so far so good bud sites everywhere.

I had a great feeling of accomplishment as I harvested Daphne today. She was the first plant I have brought to life in many years and I was impressed with how the A.B.L.E. Grow Chamber worked. I will let her dry out for a week then I will update more.......It has been a week and I am more than happy with her smoke. VERY SMOOTH no harshness. Great after taste. Even though she was a small plant she still did extremely well in all other factors,: great color, no disease, good tie down technique made for 6 tops with one main one. I will use a single 5 gal bucket for my hydro next grow to see if I get better root growth than with the 6 plant reservoir I used for this operation. The grow chamber allowed me to control the humidity, air temp, pH and PPM with relative ease. I am very happy with my first grow operation with the ABLE Grow Chamber and hope my diary was informative and entertaining for everyone. Main thing i did wrong was my fuck up with keeping the LED too close when she was a seedling. I had it at 10". (it was my 1st time w LEDs.) Secondly, I never got good root growth but not sure why. my theory is that light was seeping through the white plastic cover on the Rainforest 66 reservoir. The best thing I did was controlling all the factors needed to grow with my A.B.L.E Grow Chamber Prototype. It made great use of limited space and saved electricity with its low power usage. Look forward to using it one more time before we make the first salable model. 0

Everything still looks great 😀 The back row with the Victory Kush F1 need about 3-4 weeks. Everyone else is just starting to bloom 😎

Vamos familia, actualizamos la primera semana de floración de estas Black Rainbow de Seedstockers, salieron las 3 de 3, 100% ratio éxito. Aplicamos varios productos de Agrobeta, que son increíbles para aportar una buena alimentación a las plantas. Temperatura y humedad dentro de los rangos correctos dentro de la etapa de crecimiento. La tierra utilizada es al mix top crop, por cambiar. De 3 ejemplares seleccioné los 2 mejores para completar el indoor y trasplanté directamente a macetas de 7 litros, cambien el fotoperiodo a 12/12 y aplique una buena poda de ramas bajas, se ven bien sanas las plantas, tienen un buen color y progresan a muy buen ritmo por el momento, ya marcaron las preflores. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

6/2 Sat these girls are 26 days old from clone plugs to 4" pots, then to 3 gal grow bags on 5/18, then up plant to 7 gal grow bags on 6/1

28 Noviembre: Se realizó una defoliacion agresiva y Lollipop. Desde ahora en adelante, además de pasar a nutrientes de floración, se regará generativamente, con muy poco o nada de runoff, para tener un dryback más largo, de unas 22-23 horas, de está manera subir la EC del sustrato(pwec) tratando de llegar de a poco a 10EC, es un stress controlado para que queden plantas más compactas y espacio internodal menor, básicamente más puntos de floración más cerca unos de otros.

Iniziò fioritura,.oggi 2 agosto, secondo giorno a 12 ore...dopo aver sofferto anche lei 3 mesi dentro AD un vaso da un litro... adesso con questo vaso da 11 dovrei riuscire a portarla. A maturazione,,,💪😂

12/12/24 pulling her a bit earlier than i probably could but the largest buds are about 75% cloudy 20% clear and 5%

Very happy with how both plants turned out in the end! Pheno#1 is a definite keeper, but il be hanging on to Pheno#2 for at least another run or two. Both plants have a very similar smell it’s like a tropical cherry punch, #1 smells the strongest, you only have to nudge it and then whole room stinks. #2 has a gassy edge that #1 doesn’t have. Both smell incredible! I’ve cut both plants into individual branches and I’ve hung them in my drying tent. I’ve removed all fan leaves and any lead with a visible stalk. I’ll dry them for 10-14 days, my RH stays roughly between 55-60 and the temperature fluctuates between 19-24*c weather dependent. I’d like to thank everyone who has had a look, liked or commented on this diary, I hope it’s been helpful 🙃 Big shoutout to In-House Genetics for another 🔥 strain, can’t wait to try this one! Il post an update with some bud pictures once she’s dried and curing. Catch you all soon, until next time 🌱💚

Tannin Trail Swag

Start of week 3 Update 2/27: they have been fed with a dose that was cut in half (fox farm dirty dozen)

Ganja Farmer Seeds Caramel Auto In @highpoweredorganics new ISLAND-BLEND. Fed water soluble powders. AQUA-MARINE Grow Formula 5.1.4 paired with OCEAN-POTION and RHIZO-MOJO in dechlorinated water. Alternating ANTI-MATTER & DARK-MATTER feds weekly. Foliar fed GREEN-SUPREME daily Following High Powered Organics Island-Blend Feed Guide. https://www.highpoweredorganics.com/ Happy growing friends 🇦🇺🇦🇺🇦🇺

Plants Flipped to flower on a 12/12 light schedule. EC dropped slightly to try and reduce any major stretch. These plants should have been lollipoped last week before putting them into a scrog but unfortunately I was unable to, so I have to remove the Scrog to make it easy to lollipop and then place them back into a Scrog once lollipoped. When the Scrog was placed back on it was more for support as I'd already built the structure of the plant the prior weeks. Although a few lanky branches did get bended back over. Another Scrog layer will probably added later in flower for further support when buds start forming.

Nice start to the week, enjoying the growth on these so far. The Carl #2 seems to be a triploid mutant, should be fun to grow. Been experimenting with time lapses over the last little while and think I may finally have it down at this point. /Updates as they happen/ -Carl is more than a triploid, both seeds have four-leaf node sets -Had to support UKBS 2 with some garden ties as it got too leggy and started falling over -Watered with plain water, the seedlings are suffering under the low humidity but growth remains steady

So, this is my first grow, just starting out. After NJ passed recreational weed, I was like you know what... Went on an amazon spending spree and got some seeds from cropkingseeds and this is it! I let the seeds soak for 18hr in deer park spring water w/ 1 drop of Organic Liquid Seaweed and Kelp Fertilizer Supplement by Bloom City. At the end of soakage, it didn't look like any had cracked maybe one did not so sure. So I did up the papertowels according to the video and have to say I feel a little nervous/anxious that there is too much water/weight on the seeds and feer drownage. I'm going to keep it on there though. I check the towels a few times throughout the day and am surprised they're very moist throughout the day and next morning still somewhat damp. 24 hours in the towels I check my babies and one had cracked but the rest were still sealed up tight, unwilling to share the goodness that we all so desire, cmon babies crack that shell for papa! 11/12 looked like 1 seed popped, yay! I remoistened towels a bit but didn't wanna soak them. I also put some rapid rooters in water to soak overnight gunna throw the seeds in them in AM... I was going to do it tonight but figured I should wait see if some pop out more. 11/13 OMG upon checking seeds at 07:10 I found the papertowels like bone dry OMG WTF!!! Panic sets in but I just set up the little terrarium with 6 plugs and put the seeds in and tore some plugs off bottom of a plug to cover seedhole with. 11/14 Got a sprout! YES!! The plugs look nice and moist just going to let it go! 11/15 Woke up this mornin, saw a new sprout peeking thru and then another about to! I got ansy and took off the little top plugs and found all the sprouts were coming up! Leaving the top plug off, hopefully thats not a bad idea.

Week 16 Nothing really moved this week. It’s something like between low tide and high tide: not my favorite moment … waiting for blossoms and flowers 🙄🙏. Good point is that every plants are healthy ( except a parasite problem on Psicodelicia) and well prepared for flowering. For now it’s bad weather outside: 20 degrees max, with lot of wind and rain (cf video). The green house is the best option despite of less exposure. Morning soon I moved the plants outside to sprayed them with potassium soap (natural insecticide). I cleaned the grow space inside the greenhouse (didn’t touch spiderwebs…good insecticide too). I removed the automatic watering and get a few liters of rain water during this days. Watering 1L every 3 days -Calmag + Cannazym + Alga mic + Root booster -Flora Series: preflowering quantities. Daylight 14h30: 6h50/21h20 Blackberry Moonrocks is still healthy; no problem of deficiency or pests, diseases. This strain is resistant and vigorous: no more stretch, waiting for flowering.lot of blooming sites along the branches.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.