28.04.2024 Continue into week 9 Clean nutrient tank for the last time...This is last week of flowering for almost all girls. Electrical Conductivity (EC): 0.8 for this week.. Wedding Cheesecake: one girl finish this week, two others probably in middle of 10 week. Massive plants, tons of yelled. Creamy undertones, sticky buds. Love it! They starve whole grow, didn't have possibility for separate line just for them. Love odor and bud structure. Tropicana Cookies: Second phenotype will be harvested within couple off days. Insane plant, so frosty. It getting more colorful every day Purple Lemonade: First pheno is insane! Shape, size ,color, bud hardness, appearance, frostiness' all attributes to become photo model! Beautiful and high odor in the room! Lighting Parameters: PAR Map: Maximum PPFD is 800, minimum PPFD is 550. VPD: Ranges from -0.1-1.1 Daytime Temperature (DT): 23-24°C. Nighttime Temperature (NT): 19-20°C. Leaf Temperature: 21-22°C. Light Distance: 45-55 cm from the plants. CO2 Levels: 700+ ppm. HLG Scorpion Diablo: Set at 60%, positioned at a 45-55 cm distance. Girls drink less water, so for this week and till the end of flowering new misting timing's Daytime: ON for 50 seconds, followed by 30 minutes OFF. Nighttime: OFF for 50 seconds, followed by 45 minutes OFF. Nutrient Tank (NT): pH: Maintained between 5.80 and 6.00. Stable PH, small drifting up and downs EC: 0.87 Temperature: 19-21°C 01.05.2024 Cruse mode , observing girls, looking trichomes under phone microscope. Still have to wait. Purple Lemonade is almost ready all three girls. This is last week for them for sure. Second Tropicana Cookies will be harvested in day or two.. First and second Wedding Cheesecake are finishing this week. Third still need some time.. Almost all pistils still white, only clear with some milky trichomes Lighting Parameters: PAR Map: Maximum PPFD is 800, minimum PPFD is 550. VPD: Ranges from -0.1-1.1 Daytime Temperature (DT): 23-24°C. Nighttime Temperature (NT): 19-20°C. Leaf Temperature: 21-22°C. Light Distance: 45-55 cm from the plants. CO2 Levels: 700+ ppm. HLG Scorpion Diablo: Set at 60%, positioned at a 45-55 cm distance. Girls drink less water, so for this week and till the end of flowering new misting timing's Daytime: ON for 50 seconds, followed by 30 minutes OFF. Nighttime: OFF for 45 seconds, followed by 45 minutes OFF. Nutrient Tank (NT): pH: Maintained between 5.80 and 6.00. Stable PH, small drifting up and downs EC: 0.89 Temperature: 19-21°C 04.05.2024 EC start to raise slowly and PH is dropping . Low water consumption, girls finishing Tomorrow is harvest day for second Tropicana Cookies and all three Purple Lemonade. Last day off this week is going to be last day for Weeding Cheesecake also. Without AC unit I'm not able to additionally control temperatures.. For drying it will be little higher temps, but it is what it is.. Water keep cool for whole week, from 18c to 20c, This week watering routine is changed to 45s ON time and 45min Off time Every day perform one long misting cycle, 2min ON... Nutrient tank is top-up with pure RO water + Hypochlorous [email protected] 05.04.2024 Second harvest day.. Purple Lemonade all three girls, second Tropicana Cookies also is harvested. Lots off hard buds on this girls, frosty, fat, insanely hard buds. Odor in the room is fantastic 😋 Tomorrow on last day off week 9 , Wedding Cheesecake harvest, first Girl No.3 , then day after girl No.2. Third Weeding Cheesecake is wait for a couple off days or full week. This girl is so massive, its a pounder in yield I think!

The girls develop properly, even "Freako" promises to be interesting despite the hard experiences 💪

I get closer to the end of the cycle, the flowers ripen and smell beautiful. I flushed with water and hadn't fertilized for two weeks. There will be a scythe in 7 days. Do you agree?

Just trying to bend some branches and defoliate some bud blocking leaves. Getting close to starting a flush as she should be done in around 2 weeks. The tropical fruity funk is really some unique and great smelling can't wait to see how the buds fatten up. Happy growing 🌴

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Planted in 25L Pot with pre-fertilized compost soil and a little bit of coco, perlite and little pieces of old woodsticks. As a fly protection i use spiders and there web to solve this problem because thats the only one i have. So when you see in my tent, webs and the little bit of soil on the bottom what keeps the tentfloor dry and is also good for the spiders, please keep that in your mind.

No food just Water. Flower 12/12 on 9-12-24 = (44 days) or (6 weeks 2 days)

Overall great experience. Felt that these auto were real easy to grow, with not many issues to fix.

Got these harvested, and wow oh wow do they smell like dessert. I decided to hang dry these since the weather has been almost perfect conditions. All in all a super easy strain to grow that produces amazing flowers good for either flower or extracts. Happy Growing

25th April day 46 4 plants stripped I will give them a few days to recover and flip to the 12/12 cycle

Servus Moin! Die Pinapple Slush XL hat sich wohl erstmal erholt. Diese Woche ist super Wetter und das nutze ich, sie gleich draußen hinzustellen.

got some nice smell now! shes growing slowly but surely. have some nice little flowers and they are slowly growing. another 2-3 weels until harvest. cant wait!

The weight is coming guys

Es läuft weiterhin gut die hintere Reihe verhält sich aber besser von dicke der Knospen etc. Die vorderen sind etwas kleiner als die anderen aber denke sie ziehen noch nach. Ab Mitte nächster Woche wird nur noch Ph-reguliertes H2O gegeben und auch eventuell einmal geflusht.

11/9: I took some photos and added a 35w 3500k LED to the top section and have it pointed towards the backside of one of them and my little Stardawggy. 11/10: Today, I super-cropped about 10 different mains and branches on various plants. The canopy is now much more even. They'll be ready for a feeding tomorrow. 11/11: I fed them all about 1/3 gallon each with big bloom, tiger bloom, beastie bloomz, cal-mag, bembe, humic acid concentrate, myco/tricho, signal, and a little grow big. 11/12: I spent a couple of hours tying down branches and tucking leaves to get more light down into some of the plants, and rotated the edge plants. 11/13: I rotated the edge plants and added a TNB Naturals CO2 enhancer to the closet. I raised the thermostat on the ac infinity to 86f, so as not to exhaust it all, but with the cold air intake, it's rarely even coming on anyway.👍 11/14: Their buds are swelling up nicely! I fed them again today because I'm still seeing some signs of potassium and phosphorus deficiencies. I went 1/2 strength with the grow big, but full strength with everything else... 11/15: Last day of week 6 and I'm happy with their progress. I plucked off the dead shit and tried to reposition some of the lower branches to get more light. Took some photos and shot some budporn video..sexy ladies!😍

Smells like Rubber bands coated in a berry sugar or berry sryup very interesting on the nose! Harvested day 67 ish maybe a couple more hard ot tell outdoor Split the harvest into drying buds and some to made into fresh frozen hash. i can dry weigh the fresh frozen buds and put that weight in the wet section and i will put the to smoke buds in the dry weight section so total between the two will be the whole plant Drying was a big mishap got condensation around day 10 of drying and it caused some mold 🤦‍♂️ right around the time i was going to cure! Found a peroxide soak that ill post butt it seemed to take off all the mold without affecting tricomb heads! Had to re dry for another week afterward i feel like the re dry maybe messed with the terps because it definitely never got the smell back like i thought but who knows slmost 3 weeks into cure coukd get better but doubt it

Week 2: The Growth Phase Begins The germination phase is officially behind us, and it’s time to kick off the vegetative growth phase. I’ve uploaded a video that showcases the entire start of this grow diary, from germination to where we are now. 🌱📹 Be sure to check it out for a full recap! This week marks a critical step in the journey: Pheno Selection. I’ll carefully evaluate the three seedlings and choose the one that will move forward in this competition. So far, the plants are neck and neck, but slight differences are starting to emerge. By the end of the week, the strongest contender will take center stage. In addition to the video, I’ll be sharing photos of the seedlings as they settle into their new phase of life. Stay tuned for updates on their progress and my final decision. dein-onlineheadshop.de/ Use my referral code 420Shop at checkout to get a discount on your purchase. By using this code, you also support me, as I receive a small reward for each use. Thank you for your support!

Whats up GD !!! ------------- GROWTH------------- Topped both plants, the bigger one, it is 3 days younger than the other, and seems to have faster development, more vigorous.. I think the reasons could be the pheno or the level of root development, i ll explain it : C#1 was in a yogurth as seen on photos and having all the sun. C#2 was in a wider recipient, lower in height, and in a room with poor light. I watered both plants at the same time, C#2 got curvy leaves when watered, i think maybe she likes near dry soil, or cant handle that level of humidity in the roots ... in my opinion she has less developed roots and the pot hadnt enought holes to let the air come in, the oposite of C#1, that seems to handle better humidity and is eating all she can, getting fluorescent green below sunlight. Both plants have same feeding and soil composition. ------------- IRRIGATION------------- Watered both ladies, around 0.2-0.3l each one with Deeper Underground. ------------- NUTRIENTS------------- Tall cheese C#1 : Deeper underground 1ml/l Short cheese C#2 : Deeper underground 1ml/l + Micro Vita (it only had Canna Pro soil ) --------------------------------------- Good vibes !! _12/04/2018_