so normal grown, i've made her some LST technique for a while and respond perfectly, nothing to complain, she wa so strong , if i do it again i'll do topping or fim for sure

Bene la prossima settimana farò le piegature dei nuovi rami secondari. Il tempo nell'ultimo mese è stato veramente caldo.. temperature continue di 35+ gradi. Faccio i complimenti a questa varietà per riuscire comunque a resistere.

Week 11 harvest Chop chop down she goes What an easy and rewarding plant, she done great for my mistakes also and every person NEEDS a sweet forgiving women Amen Nice sweet spunky smell I would grow again for sure

Love my grape ape really great dna..

Another week just passed. I have reduced the main flowering nutrient by 1ml, because I’ve introduced Plagron Green Sensation as an additional flowering nutrient. This thing increases your yield incredibly! If you start looking at bud formations starting this week, you will notice significant changes.

Le transfert avec Green planets c’est bien fait et je suis passé à la dose complète Elle gonfle a chaque jour! Jusqu’à présent Green planets est facile à utiliser et j’aime comment il agit J’ai tendance à la prendre en photo plus souvent que les autres…🔥

I'm 4 days into flower. Topped all the plants nice and even. Then removed all bud sites below the top 4 on each branch. Leaving fan leaves intact. I allowed them to recover for 2 days. Then flipped the lights. Gorilla Zkittles has huge indicia leaves. The plant is growing nice and has no nutrient burn or other defects.

Day 56 Well, the Kiwi wasnt done stretching 😂 Still same settings. Only switched to bloom nutrients. The icendnbaked couldnt recover sadly, since she swapped into flower.

This lady is going from strength to strength following last weeks slug attack! I'm sure it may have slowed growth just a little while she was repairing the wound created by the slug munch. The weather has been nice and consistent, all adding to the healing process and the low stress training encountered. 👍🏻

Week 3 males developing right on time! Pollen will be dispersing soon. So now trying to nail down who daddy was? Week 3 day 1 males looking very nice, no sign of either one showing female flowers( no hermaphrodite/ true male) but I will stress them more after reveg for severe stress test. For final results. Looks like I forgot to feed mom! Shit! But I did not.I used bio tone starter 5-1-1 by Espoma gonna feed in am. With tomato tone with myco 3-4-6 it's all I got and organic to boot 👍 Last grow with Captains cake went to 11 weeks 2 - 3 applications should get me to finish line. No flush should be needed, it's feeding the seeds growth. Looking at 07/01/2018 harvest. Fingers crossed! 05/05/2018 First burst of male pollen is flying in the air, gonna try to get video of it as I shake the males. I am brewing tea with worm castings unsulphured molasses,topped off with protilizer immediately after brewing it. Give them a little love (Tea) while they are making seeds👊

Esa familia , ya va quedando menos para cortar estas hermanitas skunk de Zambezaseeds, tienen algo de sobre fertilización de las últimas semanas pero llevamos solo agua 2 semanas. Ph regulado en 6,5 . Humedad por debajo del 40% , temperatura algo alta al trabajar con sodio pues en esta época sube vaya. Así que esto tenemos y las flores se ven bastante compactas. Nos vemos la semana que viene fumetillas.

6.11.24 Gave all 1L apart from Hubba got 0.5L Ph 9.9 2.5ml ph down in 10L = ph 6.3 10.11.24 Turned 600w light to 75% power 70cm ppfd 550 changed lights to 21/3 10.11.24 Gave all 1L apart from and lemonade got 0.5L Recharge 2ml/L Bio heaven 2ml/L Acti Vera 2ml/L Root juice Ph 7.8-6.4= 1ml ph down Transplanted 2nd lemon haze in same pot.

Pilot — Week 5 Flowering Pilot is progressing through week 5 with excellent flower stacking and increasing resin production. Buds are swelling along every branch, while the open plant structure continues to promote uniform light distribution and airflow. The flowers are becoming denser each day, with abundant white pistils and a clear increase in terpene expression. The plant maintains a healthy balance between vigor and flower production, showing strong potential for a heavy, resin-rich finish during the final weeks of flowering. Seeds by World Breeders Cultivated by Piperoots 🇲🇦 🌱🔥

Hello! The girls are in bloom as well, used to spray a little bit CannaBioGen Delta 9, and thinking about a little bit defoliation to give more light for the buds, no a lot of place and looks like the leafs covering a lot of buds, but not sure. Also according to the @DinafemSeeds webpage the California Hash Plant and Critical+ has the same flowering time (45-50 days), but, it looks like a mistake or i don't know, but it seems like California Hash Plant has longer flowering time than Critical+, as you can see on the pictures and video.. Maybe @DinafemSeeds could clarify this for me as well too :) Thanks for following me and have a good grow and no stress to all you guys!!

Best flower 🔝 Strong flower. I love qualitty of HSO. everyone recommend This strain. 💚 Thank for watch. ✌️🏼🔝🔝🔝

Coming back strong.. flipped the light schedule on 8/6 and changed the formula in the RES for transition. Plucked some big fans off that were blocking lowers. Will be stripping all fans in two days, and a full strip 19 days after. Going in tonight and cutting some lower BS off so it can focus it's energy elsewhere. I should have vegged longer so I can reach that 1/2lb goal. The tent is nowhere near full, so I doubt I'll hit it this time. Will be saving a cut from this pheno to run again in the future. -Flower day 2 she's looking real happy this morning. has been drinking quite a bit and eating as well. was on a 2-2-2 formula, now on a 2-2-3 as of this mornings top off. the main stem is nearly an inch in diameter and growing holy fuck. will be stripping the big fans off tomorrow, and trimming some lower shit off. will have before/after pics F Day6 just noticed my nutrients changed from GHE to something else?? TF? General hydroponics flora series not even listed anymore? Ah well.. RES clean/change today. Prob shoulda stripped more fans the other day, but shit happens and we learn from it right??

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2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

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