The harvest is like I wanted very happy with the harvest of the blue dream up to the next diaries hydro grow pure coccos

Chop day. Everything is looking and smelling incredible. The range of smells I've got so far are: floral, grape, blueberry, gas, burnt rubber, tangerine, funk. Everything appeared fully mature beside the Cambodian x Meat Breath and Pak Man so decided this was the best time to cut them down.

This week is packing on some nodes insane! Starting to get a little resin production going going to look like some fat nugs off this plant! Just been doing straight water with molases 2 tablespoons a gallon amping those sugars threw the roof for those oils in the plant! Burned it a lil bit pushing it to the max but seems to be fine packing it on. Has a crazy smell to it idk where to put it yet. Stay tuned stuff is gana get fat and frosty!!!

Semana segue bem, planta ainda bebe bastante água.

Sie ist ein sehr kräftige Pflanze die echt schnell wächst. Mal schauen wo das hinführt...

Sorry to later publish. Done more LST

These ladies are growing great. After being transplanted last week they've definitely doubled in size. I did some defoliation today taking off the first set of branches and fan leaves that were at the soil level. All in all Happy Growing

Good morning growers🤗 End of week five.. Four days ago I transplanted this beautiful DOS SI DOS # 33 into its final 20l geotextile pot🌱🌲.. It has direct contact with the roots and I have placed some mycorrhises (MICOSAT F) which will have an action in a span of about five weeks.. Always with the aim of trying to get the best from the cultivated strain I created a potting soil that will potentially increase Brix levels🤞.. As a base I used light-mix soil of biobizz in most, more than humus and inert material, coconut and zeolite in addition to the perlite already present in the light-mix.. To this base I then added various ingredients of organic origin, but almost nothing from animals.. My recipe includes alfalfa, kelp, calcium carbonate, gypsum, phosphorite, azomite, zeolite leonardite, basalt, krill powder, and neem soil.. I will not be here to list exactly how much I put of one element and how much of another because it is a test for me too and there is everyone who tries for himself, even because the quantities to be used will vary from strain to strain🙂.. I have already made such a recipe once, but being lighter with the ingredients.. Fantastic results🤩.. The exposed plants looked like "more natural", I don't think it's a term correct but I don't know how to say otherwise🤔.. Really high quality of flowers, especially as intensity and characteristics of the terpene profile🍋🏵️🍎🍉🍇🍓🍭🍪.. But in words it is difficult to make the idea correctly.. Anyway.. From now until the end I will simply give water, without worrying about correcting even the pH.. The only external additions that I will make will be a little fish emulsion + molasses (fishmix), unsulphured molasses, probably half-flowering banana macerate and maybe I will also make tea, but I will see week by week how to behave.. For now, that's all🙌.. Thanks for reading😉.. See you in seven days.. Ciao✌️

Gorilla Glue from AMS is doing great under the Spider Farmer G3000 light. I just changed light times to 12 hours today. So let the flowering phase start 💐. I think I am past most my previous issues. My feed is pretty strong already. So I am not adjusting it yet. I will be going into more of a potassium rich diet while trying to reduce nitrogen levels once stretching is done. I defoliated today and will again more aggressively in 2 weeks. I wanted to open it up for the stretching in hopes it stays more in a bush or more even canopy. I will likely have to hst them if they stretch a lot. Time will tell of course. Thank you Spider Farmer, and Amsterdam Marijuana Seeds. 🌱🤜🏻🤛🏻🌱💪🏻❄️🌱 Www.amsterdammarijuanaseeds.com Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Spider Farmer G300w: https://amzn.to/3S2zvsd Spider Farmer 10X20 Heat Mat Kit - https://amz.fun/lsa0J Spider Farmer Amazon Store: https://www.amazon.com/spiderfarmer Spider Farmer Official Site: https://spider-farmer.com Discount code: saveurcash

Esta semana fue genial las plantas crecieron todas muy parejas..teniendo por ahora mucha homogeneidad en el tamaño y forma de las plantas

Semana 8: La Grease Monkey está en modo bestia esta semana. Los cogollos han seguido engordando con fuerza y cada vez se ven más densos y cubiertos de resina. Las flores principales ya empiezan a tener ese aspecto compacto y brillante que promete potencia, mientras que las hojas cercanas están totalmente salpicadas de tricomas, como si las hubieran rociado con azúcar glas. La alimentación sigue basada en XpertNutrients, sin cambios drásticos. Solo he ajustado mínimamente las proporciones para no frenar el ritmo que lleva. El riego sigue afinado al detalle: lo justo para mantener activa la microbiota del sustrato y evitar saturaciones. Los Adlite continúan haciendo un trabajo brutal. La penetración lumínica está ayudando a que incluso los cogollos de las zonas medias e inferiores mantengan una buena densidad. Todo el dosel está funcionando como una unidad, lo que se traduce en una floración muy equilibrada. Las condiciones ambientales siguen bajo control: 22-25 °C de temperatura, y humedad en torno al 55%. Estoy reforzando la ventilación para prevenir cualquier susto ahora que los cogollos empiezan a cerrarse más. El aroma se está volviendo todavía más intenso: una mezcla cremosa, dulce y con ese fondo diésel que le da el toque agresivo típico de esta genética. Los tricomas siguen lechosos en su mayoría, con alguna señal de maduración incipiente, pero aún no es momento de pensar en tijeras. Crecimiento firme, flores con presencia y resina a punta pala… ¡Seguimos creciendo fuerte! 💪

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Die gorilla zkittlez befindet sich mittlerweile in der blütephase

Super easy trim✂️ The buds are super dense & sticky🤩 For the smell it’s cherry & blackcurrant with a gassy touch the Cherry Cola Diesel ⛽️🍒

We are officially in full bloom. The have been enjoying 6-7 hours of sun light per day for about 4 days now, then brought back in under the cobs. The girls are all exploding with growth and stacking what I expect to be huge buds.

Starting week 6 Tent is being slowly full A little bit too hot for the next days as the weather outside will climb to 36-38°C, I thnk I will bring them some CO2. I will check with the growshop. Any idea is welcome. The stretch is really impressive, 1 of them get 20cm in 7 days Waiting for the buds 😁 Day 39 I have now added CO2 with an organic boost, it is linked it to the fan. Should be a little bit better with that temperature

Day 22 25/07/24 Thursday Still no Feed/water since Tuesday. Been a humid and overcast two days so not as much evaporation as normal. All plants happy and healthy 💚 Video updates Day 23 26/07/24 Friday De-chlorinated watering pH 6 with 5ml calmag to 5L water. Watered each with 250ml and had small run off. I will continue to feed/water with small run offs to help prevent salt build ups. Video update. Day 26 29/07/24 Monday Nice feed today, using de-chlorinated tap water pH 6. They each had 300ml with small run off. All looks incredibly happy and healthy! The Auto Kabul that was mutated and twisted has pulled herself through and is just a bit shorter than the others. Day 28 31/07/24 Wednesday End of week😁 De-chlorinated water pH 6 today with Plagron pk13-14 5ml to 5L Plagron power buds 5ml to 5L Used 300ml of the 5L solution. I will be using these on water days from now on as they are advised to be used every irrigation. We'll see how they perform 💪💚

Week 4 begins for LSD and Green Crack. Moved both ladies to their new home, 4x8 vivosun tent. They look happy and healthy. Did a heavy defoliation this week. Thanks for stopping by growfessors 👽🌳💚