Thank you. Gave her a cocktail to help with stress. Added 1st net for lateral support, not so much now, but for later. Blue light is absorbed by photoreceptor proteins called phototropins, which trigger a hormonal response that causes cells on the shaded side to elongate, making the plant bend toward the light. Try and fill this side a little. She is quite big already, just needs to find her stride again after the undue torture. 5 apex stems with 20-30 mini cola, let them develop a little, with the apical dominance shattered, all those 20-30 will all compete with each other as soon as that stretch is initiated. Key to a good stretch is making sure the plant is cycling efficiently, with large ATP conversions occurring lights out. For now, I'm keeping light intensity high. A plant will slow its vertical growth in very high light intensities, leading to a more compact form with thicker stems and leaves. This response is a protective mechanism against light stress, which can damage the photosynthetic apparatus and lead to symptoms like leaf scorching, yellowing, and brittleness. Instead of growing taller, the plant invests its energy into creating a more robust, stress-tolerant structure. Providing plants with necessary antioxidants helps protect the photosynthetic apparatus by scavenging reactive oxygen species (ROS) that cause damage from excess light. UV light exposure can impact the xanthophyll cycle by either enhancing its photoprotective role or causing damage, depending on the intensity and type of UV radiation. UV exposure can trigger the synthesis of more xanthophyll cycle pigments to increase the plant's capacity to dissipate excess energy, but it can also cause direct damage, particularly to Photosystem II, and may lead to a decrease in the de-epoxidation state (DEPS ratio) which indicates a reduced capacity to dissipate excess energy. Plants can respond to UV stress by increasing the synthesis of xanthophyll cycle pigments, such as violaxanthin and zeaxanthin, to improve their photoprotective capacity. UV-induced changes in xanthophyll cycle pigments can be linked to a plant's overall tolerance to high radiation stress. The xanthophyll cycle helps protect against photoinhibition, which is especially important when the plant is exposed to high levels of both UV and visible light. High doses of UV radiation can directly damage photosynthetic components, including the proteins, lipids, and pigments in the thylakoid membranes. Exposure to UV radiation can have a mixed effect on the de-epoxidation state (DEPS ratio) of the xanthophyll cycle pigments. In some cases, UV can inhibit the conversion of violaxanthin to zeaxanthin, resulting in a lower DEPS ratio and a reduced capacity for energy dissipation. However, the total pool of xanthophyll cycle pigments may increase, and this enhanced pool size could provide a greater potential for photoprotection despite a lower DEPS ratio. The xanthophyll cycle works alongside other mechanisms, such as the accumulation of flavonoids (UV screens), to protect the plant from UV-induced damage. Blue light repairs 100% UV-induced damage in plants through a process called photoreactivation, which uses a light-dependent enzyme called photolyase. This enzyme uses energy from blue and UV-A light to directly reverse the damaging pyrimidine dimers in the DNA caused by UV-B radiation, a key mechanism for maintaining the plant's genetic integrity. After carbon, light, water, temperature, and nutrients, the limiting factor of a plant's growth is often its own internal factors or the amount of a key ingredient. Chlorophyll concentration is one such factor, as the amount of this pigment limits how much light can be captured for photosynthesis. Other factors include chloroplast number, respiration rate, and the concentration of carbon dioxide in the atmosphere, as plants are often in a CO2-deficient condition. 60x60x18=64800seconds x 700 = 45,360,000moles. 45DLI Exposure to 165 µW/cm² of ultraviolet-B (UV-B) light for 3600 seconds = 1 hour, a extremely high, acute dose triggering stress responses and protective mechanisms. . The plant's photoreceptor protein, UVR8, senses the UV-B radiation. This triggers a signaling cascade that activates specific genes to protect the plant from damage. In response to the UV-B signal, the plant ramps up the biosynthesis of protective compounds like flavonoids, phenolic acids, and anthocyanins. These compounds absorb UV radiation and accumulate in the epidermal layers of leaves to shield inner photosynthetic tissues. The plant may increase leaf thickness or deposit more cuticular wax, creating a physical barrier to the radiation. The plant will produce more enzymatic and non-enzymatic antioxidants to neutralize the reactive oxygen species (ROS) produced by the UV-B radiation. The plant activates enzymes, including photolyases, to repair DNA damage caused by the UV-B. These repair mechanisms are critical for preventing permanent genetic mutations. While protective measures are activated, a high dose delivered over a short period can cause stress that overwhelms the plant's defenses. Photosynthesis is highly sensitive to UV-B. A high dose can inactivate Photosystem II (PSII), damage thylakoid membranes within the chloroplasts, and reduce chlorophyll content, which lowers the plant's overall photosynthetic capacity. Despite repair mechanisms, high UV-B doses can inflict persistent damage on the plant's DNA. One study found that acute, high-dose UV-B had a greater effect on genome stability than chronic, low-dose exposure. The overproduction of reactive oxygen species can cause oxidative stress, leading to the oxidation of lipids and proteins and disrupting cellular function.