This plant was harvested approximately ten days before it should have been.But this is because i'm going on vacation for 2 weeks.And I also need some weed for the next week

อาทิตที่ 3 แล้วผ่านไปอย่างรวดเร็ว ต้นไม้สมบูรณ์ดี ตอนนี้วัสดุปลูกที่เตรียมไว้เป็นเวลาสองอาทิตคาดว่าน่าจะพร้อมใช้งานได้แล้วดูจากเชื้อราที่ขึ้นบนผิว หลังจากเอาลงวัสดุปลูกที่เตรียมไว้ ไม่มีอาการใดๆต้นสมบูรณ์ดี

Weeks 6 & 7 💚 Lack of photos were taken so I have decided to consolidate these two weeks together, The 8th - 15th as well the following week of 16th - 23rd of March

So far everything is going smoothly. Last week I installed a 10L reservoir which supplies enough feed for 3 days approximately. It helps a lot.

She's coming along really well, I think she's almost done stretching, hopefully we will begin to see her bud structure fill out really well. The weather has been frigid outdoors, 11°F all week. My heater from AC Infinity is holding up incredibly against the temps. Can't wait to see this one through, I just know it'll be a good one.

Week 5 Flower. This is the start of Week 5 Flower. Grow is cruising along. Adding Epsom salts on the last 2 feeds of the week. Tip burn from using it too often.

Day 126. She is down. That girl lived 90 days from seed touching soil. Its part of my Control Garden for proving/finding RQS strain issue. For 3 weeks veged bag seed plant who spent 75% of his life not under direct light, sometimes even in shade and under most intense air fan , she is GOLD ! Cure day. She could go two weeks longer, but ... 96 g ;))) again, i can only look and admire. Crazy strain. Update on night shift ;)

Not an overly impressive strain and I would say she was not that hard to grow. If I even grew this one again I would let it go naturally with some LST. Wanted more grape flavours but they don't really come through in the smoke. There's a bit of a sharp fruity smell that's sort of piney with a hint of fuel at the end. The smoke is not too flavourful in my opinion but that just means I didn't grow it well. Super tight nugs so be careful with humidity as stated above. Also she's a bit of a light feeder so be careful with your nutes. As you can see I burnt her tips and over fed her a lot. Not a bad yield though! I've only really smoked the lowers on this one so far so that gave me a bit of a racey high, just slightly racey. I would accredit that to the lowers not being mature enough at chop. The plant is super dense and since I mainlined it the lowers didn't receive enough light. I tried to let the lowers mature more but the plant stopped maturing when I chopped the tops, this caused stress and the plant just shut down basically. Not sure if I would grow Blimburn's GDP again until I grow the real GDP.

A great week!! Chloe grew 8 inches!! Sofie grew 6!! I fed them yesterday and moved them into the flowering tent!! Looking forward to another great week... hoping they adapt to the new environment! Also, I can't believe they started flowering so early!! Unbelievable!! Otherwise, steady as she goes!

5' semaine de floraison, tous ces bien passés . Gêne sativa et bien la elles ont pris 3 cm encore . Les bourgeons commencent à grossir. Niveaux réglage culture rien de changé.. Merci les gens qui me suivent ,bonne culture à vous 🌱🌞🌱

Swell continues. Day 34 of 52

Schattenblätter entfernt. TopMax hoch gefahren und BioGrow ab jetzt weg lassen. 420✌️

Growth was damn fast and high. She's up to 115 cm. Day 5 in Flower

Dried at 26ºC and 65% rh

wet trim, nice buds, will update when more details are available --- got 0.3g of crazy scissors hash - vaped a tiny bit of it and the high is amazing 🤩 ---- 11/6/20 started curing & made some RSO of the trimming (~3ml) ---- 11/7/20 made a 2nd batch of RSO (~10ml)

The buds are starting to grow, lots of them!! PH water and substrate between 6 and 7 👍 Looks great, slow but steady! 👍👍👍 Lamp is on at night, hope to save electricity costs

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

1 week flowering,side branching seems to be minimal on this strain,hope I'm wrong.Very uniform in growth compared to each other.Signs of good Gene's.Will be feeding a fully saturated to run off in the next few days

Welcome to Bud Boutique Grow Diary - really appreciate all your love and support :) Dont forget to check out my other current grows! 🗓️ This Week: - Day 9 transplanted 2/2 with mycorrhizal Powder from Great White Shark into a 0.3 liter pots) - all still inside AC Infinity Germination Kit - keep up high humidity with about 80% and open the little air vents of the humidity dome to start with a minimum of air circulation Thank you for still staying with me 💚 ___________________________________________ --- 🌱 Strain (Sponsor) 🌱 --- 🏷️ Cookie Haze by Kannabia Seed Company https://www.kannabia.com/en/feminized-cannabis-seeds/cookies-haze --- 🥗 Nutrients and Feeding (Sponsor) 🥗 --- * 🍸 XPERT NUTRIENTS: Master Grow A+B, Master Root, Enzymes * 📅💪 baseline + additives: 2ml/l https://xpertnutrients.com/ ---💡 Lighting --- 💡AC INFINITY - Germination Kit https://acinfinity.com/grow-accessories/propagation/humidity-domes/humidity-dome-germination-kit-with-seedling-mat-and-led-grow-light-bars-5x8-cell-tray/#product-warranty --- 🏭 Grow Setup --- * 🏠🌿 Indoor: Homebox 120x120x200cm (4x4) * 📐🌀 PrimaKlima exhausting Fan 1180m3/h (running on 60-80%) & Can Light Filter 800m3/h & 1x Fanbox 1x Dyson fan for Air circulation https://primaklima.com/de/shop/ventilatoren-de/ec-ventilatoren/pk160ec-tc/ https://canfilters.com/products/filters/ All Likes and comments are highly appreciated!!! don't forget to check out my Instagram: budboutiquee - Bud Boutique