420Fastbuds-FBA2510 I'm excited as it's tester time. Germination week is complete. I planted 3 seeds and had all 3 sprout within 48hrs of being in the soil. I do soak them for 48hrs in a glass of water before planting. I can't wait to see what the weeks to come will have in store. Happy Growing

Outside Lady looking amazing

Germination was pretty good, we lost one of each strain. We soaked for 24hr then wet paper towel for 24hr then in a tray and dome for 2 days under a sun blaster 50 watt light for the first week or so

The northern lights from herbies was a best the plant was the fastest growing plant out of 10 strains. In the same amount of time as 9 other plants of different strains this plant grew huge 2 times as big and it finished up flowering after only 45 or 46 days of 12/12. It has a good smell. I only set back is it doesn’t seem to be super dense with tricombs. Don’t get me wrong the plant has plenty I would guess around the low 20% of thc. That’s not bad at all it’s just not a 30% plus thc type of plant but it’s still a great plant

Beginning a 2 week flush, just tap water from my bathroom.. nothing special lol smell is getting very dank

GORILLA KING AUTO / KANNABIA WEEK #11 OVERALL WEEK #6 FLOWER This week no real issues to report she's looking good buds are nice and dense and continue to grow her trichomes abound she's really sticky to the touch additionally she's got a sweet aroma to her!! Overall some great genetics from the KANNABIA TEAM!! STAY GROWING!! THANK YOU FOR STOPPING BY AND TAKING A LOOK IT'S MUCH APPRECIATED!!! THANK YOU KANNABIA!! 😊 KANNABIA.COM GORILLA KING AUTO

Pushing the flush, really big flowers and a sweet flavour. The plant did not drink so much in the last days hope that will not affect the flush time in relation to the maturation of the thricomes

Solo cup challenge continues to press along. Not much change, plants are in veg, growing and healthy. Still a few weeks before the flowers start so I hope to get a bit of size going before it does. Having fun and really like looking at the other entries in this contest. Check out WildeWeed, Butterstotch and BeanswithPork, silky, larimar, fireman

Good yielder, give them least 9 week flowering. Can take a good feed, i suggest to cut of N last 2 weeks. Had 2 pheno. 1 is very bubblegum type of smell and the other is just slight fruity. Harvested 3 plants. 1 was 56.20 dry at 52%RH 2 was 52.33 grams dry at 52%RH 3 was 80.23 grams dry at 55%rh Total=188.76 grams for 3 plants All the plants Peyote critical Orange SHerbert Candy Dawg where flowered under 300W Led Organic way. Total sum of grams for all plants 401.32 grams = 1.3333gram per watt Will i be growing this for the future? Nope, i'm looking for something more unique for my taste. Imo pretty generic kind of weed. Will update later for taste after 4 week cure

Day#29Update : I took off about 8-10 fan leaves although I really did not want to take anything . I pony-tailed her main top although I wanted to pony-tail each of her tops . I’m hoping this second lite defoliation establishes those lateral branches letting them become symmetrical with the main top . If this happens I’ll pony-tail each branch for a day or two then when I untie them I’ll top each branch . For now that’s the plan , make as many arms “branches” with equal growth to the central “main” branch . Day#30 update : she’s been ponytailed for an entire 24hrs which is a 1st she always breaks out of the tie . This makes me happy I timed it better . Usually I ponytail her top when it’s slightly to large/strong so the benefits are short lived at most about 8hrs which still definitely helped those lateral branches reach the light but I was aiming to ponytail her at a smaller growth so that it would remain restrained until I decided to remove it . She’s almost ready for a drink . She could go for one today but I really want to wait until she’s absolutely famished and begging for it . So I’m going to wait another day or two then water her until runoff . The last time she received water was day #24. Day#31Update: well she’s still pony-tailed ! I’m shocked and amused . I’ll let her down finally tomorrow . Made it through an entire month she’s only gotten more beautiful since fixing the VPD issue. I broke and gave her water before she was absolutely begging for it . Mainly to address the low and falling humidity in my tent . I don’t want to lose anymore precious time dealing with issues and setback on those autos finishing up in here . After I last watered the tent day #24 I did a massive leaf defoliation of the flowering girls which dropped the overall tent humidity massively . So I adjusted the intake/exhaust fans as well as the oscillating fan to allow the humidity to rise in the tent . That worked well enough until but as the girls drink slowly the humidity is back falling again into the 45s. I don’t want it in the 40s until buds are swollen . I mixed up a gallon on water and added an ounce of trace micronutrients to it and ph’d it down to 6.4 loaded it into a 1gallon sprayer & sprayed my 3 autos . Having a little less than 1/4 gallon of this full strength solution I decided to see what my Cereal milk plant was made of and gave it to her . I haven’t given any of these girls a full strength dose of anything other than the Gaia Green . At day 42 & Day 35 of the autos in flower I did a too dress of flower nutes & a week later all 3 ladies showed burnt tips so I very well may exacerbate the issue by giving them a full stenches feeding of micronutrients . “Cellie” who went into this 1 gallon pot day#9 is showing what looks hunger signs with the yellowing of the lower leaves . Maybe because I’m letting her go thristy considering there should be 28 days of food in this medium at minimum really even more considering I added more than the recommended amount . So we’ll see. Month 1 Veg went great excited to see what kind of bush she looks like at the end of month 2 and fingers crossed by month 3 she’ll be read to flip to flower and scrog down Day#32 Update: RELEASE FROM PONYTAIL PRISON so to speak 😭. She has so many tops already ! It’s going to be a pleasure and a joy to defoliate her , ponytail all her tops for a couple days , then release the ponytails & top all the main tops once to widen her up some more ! She hasn’t shown signs of stress from that full strength micronutrient feeding I gave her last night so that’s wonderful . She’s going to look absolutely crazy when I strip her bare of a lot of these leaves and ponytail those tops BUT the woman she will be once she fills back out will be worth the temporary tragedy . It will take her relatively 14 days to rebound from the defoliation I’m going to put on her if I go as heavy as I’m planning to .

Buenas farmers!! Después de pasarnos tres dias con sólo agua volveremos con la mitad de nutrientes durante los próximos dos riegos, ya empezamos a prepar nuestras plantas para la fase final ! Un olor espectacular y mucha resina espero guste buenos humos family!💚

Still no problems. Starting to fill in more and also gaining a citrus smell that hits me when I open my tent.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Still planning to take them out this Saturday. I will begin flushing this Wednesday. 10/18 added flush water. Added short video of my flower tent and some root photography

02/12/21 The plants are really coming along well - one plant may be a male (see photos for the tall and skinny one) need to consider removing this tomorrow it is affecting the lighting of the plants in its pod because it is so tall the light needs to be higher. Lights at 80% and hung 20 inches away in general. 1ml Biogrow and 2ml Bio Heaven every other day.

As always it's just a pleasure to grow this sativa dominant strain which definitely reminds me of a great indica with her extremely fruity and tropical sweet terpenes, very happy to have her in my garden and to be able to smoke such a high quality strain once again, hope you guys have enjoyed as much as me!

So, I was going away for a week. Given that I'd been babying these on the daily, I had some trepidations about leaving them for a week through at least two if not three, watering cycles. I engaged the help of a trusted friend to stop by and make sure they were watered and cared for. As I'd been running the tent pretty hot, I wanted to increase the margin of error so I dropped the power down to 75% and raised the light about 6". Once I came back, the plants were very healthy for the most part. I did begin to get PM on one of the plants as you can see in the photo. So began treating that (defoliation, cleaning, spraying w water, obsessively searching for new spots). I was very pleased with the way they were crystalizing. The one plant with the super fat colas wasn't as resinated as the others at this point yet she was THICC and dense.

we have the second week of flowering behind us. the plant grows a good 10 cm a week. i have removed the large leaves. unfortunately the german summer is a long time coming. we sometimes only have 8 degrees at night. lots of rain. i hope it's not too much stress for the plant. because of all the rain, it only gets fertiliser every two days at the moment.

First feeding since germnation. 3tbsp Dr Earth Veg, 1tbp Flower Girl with mushroom compost dressing. 1 BT foliar spray and 1 silica watering.