01/10/21- Watered All Plants 2 sections each. small leaf discolorations. Diamonds is discolored 01/10/21- Watered All Plants 2 sections each on pour. small leaf discolorations. (JT Watered) 01/12/21- New Babies dropped off (10) 01/13/21- moved new babies to other light (did not water) 01/14/21- VanHorn topped & watered some of the new plants. Also trimmed the 1st Gen 01/16/21- 1st Gen super dry and Liberty leafs had crinkles. 2nd Gen very very droopy with discolorations. Watered 1st & 2nd Gen 01/17/21- Repotted 2nd Gen to 7 Gallon & watered 1.5 gallons each. Also trimmed 01/18/21- Watered 1st Gen 1 full pour per 3 plants. Watered 2nd Gen 1 full pour per 3 plants + 1 full over all. Refilled Water Res 1/20/21 Gen 1 half pitcher each Gen 2 1.5 pitxher

Ongoing fun with my plants, hope they still doing their jobs

Today marks 7 weeks of flowering. I started flushing this morning with distilled water (1-gallon per pot).

Had them veg somewhere else 1st days in tent

- BioBizz Light Mix soil - BioBizz Nutrients used according to their schedule - stopped LST

-------------------------------------------------- Day 8 Water: 50ml RO water Humidifier: 65% (MID-MIST) Fan Speed: Mid-Low Light on @ 19:00 (23° celsius @ 76% RH) Light off @ 13:00 (19.5° celsius @ 54% RH) ------------------------------------------------- Day 9 Water: N/A Humidifier: 70% (MID-MIST) Fan Speed: Mid-Low Light on @ 19:00 (22.5° celsius @ 74% RH) Light off @ 13:00 (19° celsius @ ~84% RH) ------------------------------------------------- Day 10 Water: 50ml RO water Humidifier: 65% (LOW-MIST) Fan Speed: Mid Light on @ 19:00 (22.1° celsius @ ~75% RH) Light off @ 13:00 (19° celsius @ 70% RH) ------------------------------------------------- Day 11 Water: N/A Humidifier: 65% (LOW-MIST) Fan Speed: Mid Light on @ 19:00 (23.3° celsius @ ~70% RH) Light off @ 13:00 (20.7° celsius @ 66% RH) ------------------------------------------------- Day 12 Water: N/A Humidifier: 70% (MID-MIST) Fan Speed: Mid Light on @ 19:00 (23.4° celsius @ 66% RH) Light off @ 13:00 (19.9° celsius @ 70% RH) ------------------------------------------------- Day 13 Water: 150ml RO water Humidifier: 65% (MID-MIST) Fan Speed: Mid Light on @ 19:00 (22.3° celsius @ 72% RH) Light off @ 13:00 (21.5° celsius @ 67% RH) ------------------------------------------------- Day 14 Water: N/A Humidifier: 65% (MID-MIST) Fan Speed: Mid Light on @ 19:00 (24.5° celsius @ 63% RH) Light off @ 13:00 (22° celsius @ 65% RH) -------------------------------------------------

Removed a few fan leaves.✂️🌿 Changed the nutrient solution. 🌊💉 Very happy with this strain so far. 😁👍

@ 1.2-1.3 kPa

Rain week 🙌

So far it seems to be fine even if the high humidity worries me even if the temperatures are still high then they have stopped growing and will finally focus on the flowers and slowly we recover these small deficiencies

. 🌱 : Defoliation + LST on day 32 💧 : 3l day 30, 3l day 32 💡 : Dli: 40 mol/m²/d 🤔 :

2024.09.12 D64 Its been two busy week, as i trim the 1 pond mother, get aknowledge that my wife use my grow sniper two pruned is badly infested rose bush, since 2 week and never proper clean it whith alcool.😱😱😱😷 in result a massive PM & mithe contamination in my grow tent... 😱😭😷😭 all plant have been shoot with H2O2 at 0.5% and set ouside for 2 day, wich turn to be rainy as f***, until i clean and serilized everything, twice and run the hepa filter .😷😰 the plant look ok, lightly over wartered, but no more pest or PM sign as they return in the tent.. 3 days later PM hardly bonce back, no choice to use more agressive treatment so they all have been shoot with saffer's defender 3 in 1. after 3 shoot in 24h and another tent desinfection we should have get rid of it 🙏 8 day laters everything look fine👍, all damage leaf have been removed, i also prune some unrelevant lower and inner grow

Screen is set at 11 inches plan on flowering at 20 inches at second trellis net, today went in defoliated and topped a few and made a few more clones deciding if I should fill with 5gals or 3 gals

Flowering day 22 since time change to 12/12 h. Hey guys :-) Another great week that is over. They all develop exactly as it should be 💚. Since the last leaf and substrate application against tripse, I finally haven't seen any of the little biters 👍. Watering was done twice this week with 1.3 l each time (see table above for nutrients). The lowest shoots were removed so that the energy is not wasted in the lower part :-) . Otherwise, as always, everything was cleaned and checked. Fresh osmosis water was filled with stale tap water in a 100 L tank for the coming week 👍. Have fun and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Strain at https://pcg.international/shop/pcg-int/ Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany

Still stretching but also flowering nicely, started her on advanced nutrients big bud today at full strength along with Ionic grow just for the last stretch. Bit worried about the EC so might drop BigBud down to half if there's any burning tomorrow fingers crossed she's fine.

Some lights out pics in this weeks line up! Another top feed 70/30 again

What do you guys suggest I give them for nutrients, I have only been giving them silica blast in their sohum medium.

Booom! Ya tenemos los cogollos formandose Farmers!! Ya casi no se nota la falta de magnesio por problema con el humidificador pero en breves ya empiezo lo buenoo!😜 mis favoritas las de Barneys Farms!!! espero que os guste farmers buen comienzo de semana!!💚

No training yet weeks 3 thru 5..

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.