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@Dsant
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Went on a holiday this week, left her with a new solution at the end of W6 and changed it at the start of W8. Another reason why I absolutely love Autopots.
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Flowers are starting to get thick. Still watering the same amount and everything is going great! The smell is definitely getting stronger too.
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I harvested when go began to see some amber trichomes, the plant grew very well, the maturation of trichomes was quite slow. The buds are nice and full bodied and the scent is very sweet and pleasant, reminiscent of gesolmino and strawberry bubblegum.
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As the plant enters the fifth week of flowering, the first trichomes have begun to form. The buds have started to thicken considerably during this week. They are becoming denser and more robust, which indicates that the plant is channeling its energy into producing larger, more resinous flowers. Throughout the growth process, the cannabis plant has shown no signs of health issues. It has remained vigorous and resilient, with no observable deficiencies or diseases. To ensure optimal light penetration and airflow, the plant was lightly defoliated this week. The cannabis plant is progressing well in its fifth week of flowering. The formation of trichomes, the thickening of buds, and the plant's overall health are all promising indicators of a successful flowering phase.
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@Salokin
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Hi Growmies, Not much to report, other than that her buds are coming along very nicely, filling the colas and starting to cover herself in trichomes. She is extremely thirsty, finishing up to 1.8—2l a day. I fed her the last tester of rokzbastic and will afterwards go down with the nutes. Still defoliated slightly as I wanted to get the colas a bit separated for aeration and light penetration. I have also added a pic of the orca bottle I use, just in case ;) Thanks for stepping by and hope to see you next week.
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Day 20 - All the girls doing well except one which was literally slow from germination, so ill be pulling that one and left with 3 Runtz. Aside from that, the rest of the plants are loving the environment, the cooler conditions definitely helps. Also have a thermostat that kicks in if its too cold and cuts out if too hot. Will start a little bit of plant training but tempted to just let them run and grow out part natural with a bit of assistance.
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#seedsman420growoff and #SeedsmanSeeds 📆 Week 15, 3-9 August 2024 3-9 August - Observed and let the plant grow. 📑 Pineapple OG performed well this week! Her water intake has slowed down, one of the final signs of maturity. About 4 more days of a light nutrient feed and then a fresh RO flush for 2-3 days. Harvest is right around the corner. 🍶 6 Aug nutrient solution changed 🍽️ 6 Aug feeding schedule updated 💧 Using reverse osmosis water with EC/TDS at 0 🐉 Nutrient Solution EC 1.9 at 74 degree F 🔆 Light power at 65%, DLI 40 canopy coverage at 12hrs 😤 Using General Hydroponics, HGC728040, Dual Diaphragm Air Pump, 320 GPH That is it for this week. Thanks for the look, read and stopping by.
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She's developing very nice on her second week, she's not developing as good as her sisters but she's definitely coming along. Let's see how she performs. 💎💚👨‍🌾
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Mix packs and Gorilla Z were fed Dr. Earth w/worm castings and top dressed with compost. All plants received full strength Silica Boost foliar spray. Mix pack#2 showed 2 unopen pollen sacks (?) which were removed.
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Day 40 she’s beautiful and huge and just perfect. I’m very excited for her and how she had progressed so far.
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@AlexS
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Week 8 day 51 1 day left to harvest the first one🤩🤩 I think the rest will be ready at day 56.
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@Waveform
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Day 17 (starting Aug. 2nd afternoon): Will water them with a lightly enhanced RO water that was imprinted with frequencies via a PEMF coli for about one day when their day starts. After being away over the extended weekend, on day 20 ladies have grown very nicely. I topped nr# 1 and #2 after I took the photos and will give them a bit of water with Root Complex to compensate for the shock later. Sadly my brilliant control equipment has crashed completely and will not even take a photo currently. Yikes. Hope I can get it back alive this week again. Also turned the left light up by another 20%, now running at 40 W, and lifted it a bit. Eye inspection told me lights were lower here and ladies can get a bit more. At the end of their day 20, they seem to have survived the topping well. I managed to cut right above the new nodes which are already growing. Ladies are at 20 cm height before going to sleep. Day 21 shows great growth again, with lady #1 being at 22 cm now and lady #2, while being a bit less tall, extremely good side branch development. I hope that the rearranged lighting on the left side of the tent will make #1 grow a bit bushier too. Besides from that, I am really happy I tested Hesi HPE. I had such enormous success with room plants that made me want to see how Cannabis plants react to it. It will not add much to salinity, so I dared to add it quite early. While the photos are processed to compensate the lighting, I did not add much to saturation, so leaves are indeed of a very vivid, luscious green and ladies looking extremely healthy. Speed of growth has increased too as the added video shows. It also tells me lighting is enough – they are turning their leaves away right at the end of their day. I am really uncertain if I will change to usual Hesi grow fertiliser at all … Did a bit of LST to the lowest branches of lady #2 which spread almost to the border of her pot. Both temporary girls will leave the tent soon, so that the competitors will have maximum space to develop. Day 22, end of week 3: Outside girl went to get fresh air from now on. Wishing her luck, good weather and not a single male in the vicinity. I rearranged the lights and positioned them a bit higher. First preflower signs appeared on the ladies, so I will change the lighting phase when I return next week. Currently, I have them at 18/6, 45 cm above heads, with 80% running to a total of 80 W. I wasn’t so lucky topping #2. She has only one "top side" branch now … But grows extremely well, so ok!
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.
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Harvested at day 85. I was a bit busy and didn’t take care of my girls enough, but they somehow managed to offer me very good yield and amazing terpenes! I can totally recommend this strain for everybody, who feels themself a cannabis connoisseur like myself😋 Dry weight is 182g and one of my plant turned out to be purple and got a really sweet smell and taste like candy and purple berries🍇 After curing I hope it gonna be more tasty, but I can just barely feel that hay bouquet. In conclusion it is a very forgiving plant, fast, beautiful and taaaaaasty, good to use LST and picking off lower bud sites early gonna make big fat buds on top and a little defoliation too. Thanks for everybody who followed my grow or see right now. Remember you are loved and you are not alone, be yourself and be kind to plants and animals they are beautiful creatures!🙏🏻 Happy Growing🌱
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Se me descalabro el medidor de ec, otro dwc que estaba a más de 3 de ec . Calibre el medidor y solucione el problema.
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I've added a video review about this strain, and here's the information about Beary White from mephistogenetics.com: -Beary white is a medium sized variety, she doesn't veg for too long before rapidly entering the flowering phase. She has a nice balance of resin coverage on both leaves and flowers. Flowers grow nicely compact and the quality is great. She is a lovely all-rounder in many aspects. -Effect - Strong, Happy, relaxed, but not too inda-couch. -Medicinal Benefits - Great strain for pain and stress relief. Thank you so much for the seeds to grow this great tasting strain. @MephistoGenetics #MephistoGenetics | October 2017000
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@BunnyBud
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Photoperiodic ones are definitely far more satisfying.
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Hello Diary, Titan F1 is developing incredibly fast, I think he will be the first ready to harvest. It reached just over 50 cm in height, which is great for smaller spaces like mine. It has a nice pronounced main cola and branches filled with nice hard flowers. She looks really impressive. The leaves are of a healthy dark green color, the smell has become more intense, which gives me special pleasure when I open the grow box. Watering has become more frequent, every three days each plant on the Farm receives about 2.5 liters of water. I continue adding Bio Flowering Booster and Easy Bloom tablets. Conditions on the farm are good. The temperature is around 28 degrees, while the humidity is around 45%. Here's what it looked like last week. 06/06/2023 - Day 38. Watering. I added all three components from the Bio Flowering Booster. pH I lowered it to 6.0. Each plant received about 2.5 liters of water. 09/06/2023 - Day 41. Watering. This time I put one Easy Bloom tablet in 7 liters of water and watered all three plants on the Farm with that amount. 10/06/2023 - Day 42. End of the sixth week. I photographed the plants and removed three or four yellow leaves. Titan F1 - 52 cm That's all for this week, thank you all for your support and see you soon.
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Bueno familia ya finalizamos, estas red hot cookies son una cepa bestial. La verdad que el secado muy bien 7 días en Malla y a los botes, 45% humedad y 23 grados es la temperatura ambiental que han tenido en el secado. 4 plantas que se cultivaron en un armario de 1.20 pero estos ejemplares solo ocuparon 80x80. Así que ya está cultivada esta gran variedad que es novedad este 2020, americana de la familia Cookies, la Tropicanna Cookies (Girl Scout Cookies x Tangie) con un porcentaje de thc entre los 17/25%, una bomba. Que decir de Agrobeta, lo peta 💪 en cuanto a nutrientes. Un saludo y buenos humos.
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Apricot Auto has really started to throw the trikes and frost up! She’s gonna start putting on the chunk really soon. She’s smelling absolutely amazing and when you squeeze her your fingers will stick together🔥🔥.