2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

un peu moins de 5 mois de culture pour ce résultat, c'est long certes mais rendu sympa Faire attention à l'humidité, ventilation si indica ... mais sinon globalement tout à été très bien . A voir quand cela est sec .

7/14 4AM MONDAY MUST DEFOLIATE!!!!! I HAVE 4 INSTEAD OF 2...BECAUSE OF MY LITTLE ACCIDENT EARLY ON.. I WILL TAKE ONE OUT OF FLOWER AND RE-VEG TODAY!!! 7/15 420am They are great cutting I took last month or week is great. Amino and mb today!! 230pm spectacular results today heavy defoliation I removed branches. I prefer a four cola grow in small spaces. It’s just better for airflow better for the plants and they responded so great. It’s like they’re jumping straight up now. I’ve never seen this. They’re just so vibrant that means all the amino’s. Everything is working perfectly. 👍👍👍🚨🚨🚨🌿🌿🌿 7/16 320am 🌿🌿🌿🌿🎊🎊🎊🎊💥💥💥💥👍👍👍👍 7/18 3am NOTICING Elongated Sepals!!!! from Search Ai "Elongated sepals in plants refer to sepals that are longer than average, often appearing narrow and pointed. This characteristic can be a defining feature of certain plant species, such as the long-sepal beardtongue (Penstemon calycosus). The length and shape of sepals can vary significantly between species and even within the same plant, influencing the overall appearance and potentially the function of the flower. Elongated Sepals: More Details Definition: Sepals are leaf-like structures that form the outermost whorl of a flower, typically green and protecting the bud. Elongated sepals are simply those that are noticeably longer than the typical sepal length for a given plant or group of plants. Examples: Long-sepal beardtongue (Penstemon calycosus): This plant is named for its elongated sepals, which are a key characteristic. Berberis (Barberry): Some Berberis species exhibit elongated sepals, with variations in shape and length among different sepal types within the same flower. Significance: Identification: Sepal shape and length are important features used in plant identification and classification. Pollination: In some cases, elongated sepals might play a role in attracting pollinators or influencing how they interact with the flower. Protection: Sepals, even elongated ones, still function to protect the developing flower bud. Morphogenesis: Studies on Arabidopsis have shown that the shape of sepals, including the presence of elongated tips, can be influenced by mechanical feedback within the plant and the arrangement of microtubules."""🚨. THIS IS ON 2 OF THEM, SO I MAY HAVE 2 STRONGER THAN AVG. PLANTS. I WONDER IF THE MB, AMINOS ARE HELPING WITH THIS TOO??? 7/19 I GOT A MAGNETIC STIR THING. 150pm they didn’t need a lot of attention today. They do need to be trimmed back down to four colas. I’m running out of space they are very perfect and if I had more space the leafs would be bigger, but that’s limited by mine condition so there’s nothing I can do but they are absolutely incredible🎊👍👍

*Increased Ppm from 750 to 800 *1st week of flowering actually Tomorrow is :D * I got different phenols. Ones short, ones wide, and the other one is a mixture of both let’s see which one comes out better.

Día 31. Las plantas pegaron un estirón muy fuerte estos días. Estoy pensando en pasarlas a floración antes. Pero no quiero mudarlas al principio de la floración, para evitar stress. Sería lindo tener una referencia de cuanto ganan en altura en floración. Si es el doble en altura, no va a haber problema. Pero si es el triple o más, cómo muchas variedades que he hecho, se puede complicar de poco a mucho. Ya corté esquejes para intentar mantener las dos variedades. Voy a ir mirando, pero son 10 días más de crecimiento y de raíces que van a tapar todo.

D22. Defoliated, gave plain RO water and dimmed the light. Next is auto irrigation. D23. Finally received all of the Athena nutrients and fed for the first time. D25. Removed the small runt plant with the twisted stem. The plant started growing pollen sacks. D26. Raised light as much as I could without using zip ties. PPFD now 650-790. EC tonight 2.4.

Esta semana tuve dudas con el riego, como en casi toda la etapa de la planta, pero al fin, la veo super bien. Le doy 1 Litro de agua cada 3 dias, y parece que le encanta! Las ramas principales miden entre 70-80 cm, la mas grande llego a los 90 cm ! Ya no hay lugar para que siga creciendo mas alto, ya que no puedo subir mas la luz. Excepto ese asunto, todo va de 10 puntos! Ya estoy saboreando mis primeras cookies. Paz!

March 24 - 30 Pretty mellow week with final prep for leaving them unattended for 5 days. Drizzela is showing signs of a lime green discoloration in a small area to keep an eye on. Blumat is steady and dialed in. The plan will be to water over the Blumat system with recharge mixed in which will prolong calling for water from the reservoir.

Onto the next week of flower. Going on a short trip away for 2 days from 17-19th, so the reservoirs will be full before we leave. Got back to more growth and had to raise the light up. Ladies looking great and getting big. We are a few days before lollipop so the tent is a bit humid but not bad. It will be easier to keep VPD at 1.2 after day 50 or so when some of that unneeded growth is out of there. Lollipop done on 21st and 22nd. Next on 23rd to finish up. Things are getting frosty with the CropSalt Bloom nutrients. Ladies didn't mind the haircut one bit Buds are starting to swell!

Gush Mintz clone

Discount code NINJABUDS: Air cube GROwACE.com Discount code NINJABUdS: ELUfAH Everything looks healthy for now. Nothing big to update yet I have not chosen which 6 plants I’m going to keep yet. I’m only going to keep one plant of each strain

We start growing again, next a really hot summer!! Peace!

Ich werde einen Komposttee zubereiten ich finde, dass ihm Nährstoffe fehlen.

Harvest Notes ✂️ • Harvest Timing: Papaya Zoap was harvested at day 63, with select buds taken earlier at day 56 due to mold concerns. • Drying: After careful trimming, the buds were dried for 12 days. The quality and aroma during drying hinted at the stellar smoke to come. • Density & Yield: Buds were rock-solid, delivering an excellent yield with perfect density for storage and enjoyment. Terpene Profile & Aromas 🍋 • Primary Aromas: A tropical explosion with sweet papaya upfront, complemented by creamy, soapy undertones that make her truly unique. • Complexity: As the buds cure, a hint of citrus zest and exotic fruit emerges, creating a full-bodied terpene profile that’s simply irresistible. Cultivation Experience 💡 • Highlights: Her uniform growth, heavy trichome production, and response to super cropping made her a delight to grow. She thrived under careful spectrum adjustments and defoliation, showcasing her adaptability and vigor. • Challenges: The dense bud structure made her more prone to mold, especially in a high-humidity environment. However, proactive monitoring and swift action mitigated the spread. Verdict: 🌟🌟🌟🌟🌟🌟🌟🌟🌟🌟 (9.8/10) Papaya Zoap is a strain that commands attention with her beauty, aroma, and unstoppable growth. While her susceptibility to mold required extra care, her overall performance, yield, and quality were nothing short of exceptional. A grower’s dream and a frosty masterpiece! Stay tuned for their individual harvest reports, smoke reviews, and more as we wrap up this epic series! Discount Codes so you can save big on your next check out 💚💚💚 Kannabia - DOGDOCTOR 30% off SeedsmanSeeds - DOGDOCTOR 10% off CannaKan- DOGDOCTOR 15% off terpyz.eu - DOCTOR 15% off The Neutralizer - PORKIT5-DOG 15% off Fast Buds - DOGDOCT 15% off As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and so joyful with you all in my life 🙏
 With true love comes happiness 💚🙏 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so 💚 Friendly reminder all you see here is pure research and for educational purposes only Growers Love to you all 💚💚💚

May, 8th I tried to do different Trainings on the Cookie-Plants the one on the Pictures is growing quite Wild with alot of laterals but atm i let her do the Thing The others have a more strict Growingregime✊ All Girls sit in amended Soilmix, ( EASY BOOST ORGANIC NUTRITION) so only Water and Beneficals for them waiting little before I start Floweringnutes

welcome to📅 Day 8 6/14/2021. Things are going just as I would expect for this stage and no gnats as of yet thank god for that🙏 If things go as planed top feedings will stop by the end of the week and I will move on to the reservoir on a 2 week cycle. The Mars Hydro TS 1000 is performing just as I would expect and that's to say flawlessly this little light works its magic best in the veg faze of the grow when growing multiple plants but in flower it will need some help. If I had 2 of them I think I would be fine in my 4x4 tent one for each plant or better yet the TS 2000 for each plant Until Thursday Happy Growing and as always Keep your stick on the ice 🏒 Update 📅Day 11 6/17/2021 she is well on her way and I am very happy with the progress. My Mars Hydro TS 1000 light is preforming just as I would expect and I have turned is up to 75 %

I am back from my holiday left on 19th of December so my friend handled them in had to remove a lot of leaves atvteh bottom they are very tall pistils are out. Crazily nice! :)

wonderfull colours and smell, plant is looking fine, i see another 2/3 weeks from now, for a full development of the buds. heavy and frequent rain occurring, didn't have to water so much, hope it get better from here to the cut. i am gonna decrease on the nutrients, to make them disappear in 10-15 days and a have a full week on just pure water. the quality of the smell is incredible, very sugar-candy like, not so citric.