At the start of my first proper week of growth she seemed to be doing really well. I noticed her stretching quite a bit, so I moved to the light a bit closer. This seems to have done the trick. Thanks to everyone on Grow Diaries who answered my question about burying some of the stem when transplanting. I decided to move to a slightly bigger pot (from 1litre to 3.8 litres) so that I could bury some of her stretchyness. This was based on advice from you guys - so thank you! She seems to have taken to the transplant well. I made sure the EC and PH of both mediums were very close.

Well, it's finally time for an update, right? And for a newbie like me this week has been quite eventful! 😲 After last week's "Helmet Head" (which I suppose was my fault for not burying the seed deep enough 😱) her leaves started to look twisty, Honestly don't know why that happened but my three hypothesis are: because that's just the way she is, because I didn't properly bury the seed or I made a mistake somewhere else. However I also think she looks pretty and this makes her look quite "special" 👍. In her 6-7th day after sprouting I noticed her leaves started to look even more twisty and she was about to outgrow her cup. I worried and decided that it was the right moment to transplant her. At first I didn't know "how" to do it and all I knew is that I could shock her if I did it wrong (which worried me haha 😨). None of the sites I checked really specified any kind of information regarding how I should prepare the medium for the new plant (just some vague information here and there) so I came with the idea to just water it bit and then "massage" the soil to avoid making it "lumpy" and I feel it did work out well in the end! I feel I didn't shock her much and in her second day she was growing even more as well! Nice! 😍 Next week I'll start feeding Puff with some nutrients but the PPM of the tap water where I live is somewhat high (around 600) and it's electroconductivity is around 1200 us/cm but a lot of growers in here still use it (they just wait for chlorine to evaporate) so I'll try doing the same. I'll use half dose of Biobizz Bio Grow and half dose of another product called Gold Sunshine from Quemanta (which is Cal-Mag with some amino acids) and see how things develop! 😃 I'll try to see if I can make my DSLR take the photos for future timelapses, because my webcam sucks for that job! Thanks everyone for reading 😉

In dieser Woche wurde die Pflanze nicht mehr besprüht und die Luftfeuchtigkeit ein wenig gesenkt 💦 Die RP43 ist in dieser Woche sehr gut gewachsen , deshalb wurde die Dünger Gabe erhöht . In der ersten Woche war es ca. die Hälfte ☘️🌱 Ich denke sie wird noch 1 -1 1/2 Wochen in der Veggi sein bevor ich das Licht umstelle 💡

Let’s go day 43!!!! Week went real well , girls started preflower so sometime this week I will be switching up the nutrients for flowering! We are stable and looking super healthy! Can’t wait to see what these ladies do this week! Hope you all enjoy !

Week F3: 11/04/2025 – 17/04/2025 12/04 & 14/04 – 2L nutes. Minor branch LST. 17/04 – Major defoliation. 2L nutes. Risk taken but plant strong. I was hesitant about this late defoliation, I should of gone harder prior to flowering, my mistake and easily avoidable with experience. All that in mind, there is ample room for airflow. Currently cool air is being brought in through the bottom of the tent, circulated via the internal fan, then pulled up and out through the exhaust fan system, air is then easily vent out of the external rom to manage humidity (also done by using an extractor fan - can you guess where it is yet?). I think the stretching is coming to a close, finally, almost ran out of headroom...

This strain is growing fast and flawless so far they are stretching alot more than expected and hope that stops soon running out of room after 4' tall wich is 5' tall from the tent floor was 2' only last week

09/07/2021 (F DAY 42) El olor es sumamente intenso, todo marcha muy bien! 13/07/2021 (F DAY 46) Continuo con los dos riegos semanales de 3 litros de te y las plantas están super contentas, Sweet cheese ya está amarillentando y es sin dudas la mas rápida. Pomelo Haze es la mas lenta y espero tenerla 1 a 2 semanas mas dentro del indoor luego del corte de las damas.

Growth was damn fast and high. She's up to 115 cm. Day 5 in Flower

Week 5 (Day 35) Flower- the week was uneventful. Building stacks, no issues, it’s been rainy here so the humidity has been high, the old dehumidifier is getting a workout 💪 I lowered the light an inch, might not seem like much, but I’ll watch and see if they can handle it. Full feed of nutrients tomorrow when the lights come on😎

Background Fingers crossed that these Spicy Bitch regs provide at least one male so that we can back build some stock of it. In the event that does take place we’ve got a single clone of each strain from the previous pollen chuck to sit in the tent with them so they can make babies for the next few months. The whole point of the last year was to start making some foundational genetics for a third generational cross we could call our own. If you’ve been following along than you’ll know that it’s been anything but easy. Our previous pollen picks didn’t pan out at all so we’re back to the drawing board with a new option from ExoticSeeds. This’ll be a 10 plant run with 5 regs and 6 strains for pollination including the strain itself. We’re running in a 2x4 MarsHydro grow tent all powered by a Mars Sp6500. That’s a little more than 600w packed into half the recommended footprint😳. Why? you ask - because we like our buds big and overcompensatory😂. Really, I’m just of the mind that we can pack more light into this space than recommended and get much better results. The math doesn’t lie. At least not as long as I wasn’t high when using the calculator again….. Many thanks to @MarsHydroLED for the opportunity to test run the equipment👊. In addition, we’ve also moved to a new nutrient system - Druid Nutrients out of the Netherlands. They don’t really have a presence on GD yet but you’ll be hearing about them very soon I’m sure. The beauty of this system is that it’s literally a 1 (one) component system for the entirety of the plants grow cycle. It really has changed the game in terms of ease of feeding and maintenance. It’s also very versatile and seems to provide everything needed across multiple different strains in the same ratios. Keep your eyes peeled for these folks. Pretty much a guarantee that they absolutely blow up once more people know about them👍👌😎. Strain Background Spicy Bitch: predominantly sativa, heavy yielder with a daytime kinda trip profile. It’s a cross of Queen Mother and a Pre-2000 AK47. We’ll pull what pollen we can and hope to have a female run out a larger supply of seed for the future. Many thanks to @Exoticseeds for the chance to give these beans a go👍👊. Pollination Strains: Holy Surfer S1 - this is one of the focal points for this cycle. A fellow growmie was kind enough to ship over some S1 genetics I took an interest in. The only ask back was that if I did cross it to send a few his way for testing👍. Shoutout and cheers to @blendmedmedman. Hoping to have something back to ya in short order. For full details on this alluring hybrid option check my buddies link at seedfinder - https://en.seedfinder.eu/strain-info/Holy_Surfer/Smiling_Tiger/ Northern Lights - this came from a particularly interesting batch of seeds that have shown a very dependable variegation trait. It’s a compact and squat plant requiring very little maintenance. Typical for the strain really. It’s been chosen as an option to potentially mellow out the high , shorten the stature of the resulting hybrid and inject it with some of that mellow indica effect. Boogie Nights - total shot in the dark. Still haven’t even tried the strain myself yet but it was available at the right time and interesting enough to make a salad with. Big thanks to AMS who’ve been nothing short of an excellent seedbank to collaborate with. Expect similar growth characteristics to that of the Northern Lights. Better resilience however. Blueberry - this particular clone is from extremely strong stock. Spliff seeds broke the mold with this strain. She’s lower maintenance than the NL, more resilient than anything else we’ve got on the menu and the terp. Profile on this girl is out of this world. If we can bring any of these qualities to the cross, then it could be magic👌. White Widow - garrghh, I just can’t get away from this particular plant. Again, still haven’t even tested the buds yet and all I can say is that the growth characteristics are extremely vigorous and desirable. This plant is one of 3 unicorns that I have yet to tame due to a combo of extremely bad luck and circumstance. All told, it’s still the same story: a VERY experimental breeding project designed to shotgun a broad swath of genetics against a reliable and dependable Breeder who’s put out proven fire 🔥🤟😎👍.

Hi guys Entering week 6 on the 4 strain fastbuds 420 auto tent Grown under the marshydro tsl2000. Major defoliation done yesterday and lollipopping. I no there autos and you shouldn't do this 🤣🤣🤣 never affectes them in my opinion. Few plants are in need of some calmag so I will give them some tomorrow. Some nice colors on the cherry cola 💜 ill keep you all updated.

Genética de Jack Herer en cultivo 100×100 orgánico, en éste caso con una poda apical realizada y con muy buen resultado. Grandes flores cubiertas de tricomas hasta el punto de formar gotas de azúcar, es impresionante. Sabor diferente, muy especiado, con un efecto eufórico y creativo, gran pegada.

Scrog netting is becoming a challenge, I am trying to fill bottom right corner but the plant continues to grow in all directions. The areas that are filled keep growing, I have stopped topping and keep tucking. I have put 4 clones in the corner because I do not want to wait anymore as my 2x4 will get too big during the flower cycle of this tent. Turned off light and will keep off for 48 hours, officially starting the flip into flower cycle. Plants look good, no issues so this is the perfect time for me. Wish me luck, I hear this strain loves to really stretch. Fed nutes at 50%, they just got off a lockout so taking it easy, they'll tell me when to up the feed.

Sour Diesel Haze is coming along nicely, lots of upward growth and node development. I gave her a FIM pinch this week. Expect the flowering stretch to start at any time. 👍

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

For fweek 11 she’s looking great with more and more ripening of the buds getting fatter every day and insane thrichome production she’s quite small build size because of the lowerlevel of scrog bit her width makes up for in height with the thriches and pistols starting to change colour getting fatter and fatter getting closer to flushing date

Week 6 already! The buds are getting more and more tricomes and slowly getting fatter, we also gave some PK but i think it was a little too much combined with the palm tree ashes in the soil, but nothing to worry about, we wil lower the dosis and start the flush next week 😁👌

The tricombe production on this strain is ridiculous! It looks amazing under my microscope. The heads are mostly milky coloured so this may be finishing sooner than expected. The space in the tent is getting a bit tight so I'll be glad to have more room when this lady has done her thing.