Week three of flower is off to an amazing start! At day 22, the ladies are looking incredibly healthy. They're praying like crazy, reaching for the light, and stacking nodes like it's their job. The buds are starting to plump up, and the trichome production is getting intense. I'm so excited to see how they develop over the next few weeks!

5. Woche Hab sie letzte Woche nomma getoppt und jetzt darf sie sich erstmal bisschen erholen. Update von Tag 40: Topping nr. 4 ist durch und jetzt darf sie sich erstmal erholen 😊

Currently have my first Grow finishing up, two bag seeds was told they were Pink kush and death Bubba they are coming out looking as the strains are supposed to. Lights will be switched to 18/6 in a week or so

Some yellowing leaves not sure if it's it's starting to fade or it's nutrient burn or a ph problem,...🤔I will figure it out...🙄

Just look at it. All deformed and shit. Already has a fat stem on it.. maybe the nutes should be toned down? God knows. 4/2. It's like it's growing sooooo many leaves... plain water at end of week. Contemplating defoliating it...

This girl has surprise me tremendously because for an auto plant it has a fairly generous vegetable time and with genetics that make them grow very big and tall and deliver such beautiful white snow coated buds looking forward for this one looking and smelling great. Thank you for reading I will continue to update have a happy grow

A white christmas for me after all! 😂 Week 13 harvest! Plant 1 = 67 grams/2.3 oz Plant 2= 55 grams/1.9 oz overall= 4.2 oz (5-7 day dry) The Gorilla Cookies are soooo damn frosty and stinky…i’ll be drooling at them for a month until I can taste them!!!! Photos mostly of plant 2..1 was coated in resin but plant 2..the frostiness can be seen from far away! My first top shelf grow 😈…hopefully more to come! My glove was literally sticking to the stems and getting stuck with plant #2 The stench reminds me of thanksgiving dinner LOL..sausage stuffing and gas is what comes to mind..very savory aroma! One plant flushed partially the other not flushed and I can tell you that the flushed plant has much more flavorrrr..if this didn’t prove to me that flushing is absolutely essential IDK what would! LOL

The plant is starting to show signs of early bud sights, and producing large fan leafs as well as good healthy gassy smell

steady as she goes.

Day8: First day of the new week. I assume still pretty much focusing on roots, as the top didn't really do much today. Day9: Nothing special. But she's growing Day10: Still roots, it seems. Very little happening up top. Day11: Effort being put into growing the top leaves. They increased in size. I assume that the next node will start tomorrow - kicking off the switch into Veg. Day12: Next node already showing :D Day13: I put in a humidifier and changed the cycle of the extractor to keep more moisture in the growbox. My humidity was way too low. Day14: This time the humidity was too high. It was sitting at around 95% when the lights went on.

Girls are doing well with the LST. Kosher cake was topped last week and now has what looks like 8 heads. 3rd of June have given the girls living soil veg.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

W3

MIMOSA by ROYAL QUEEN SEEDS Week #7 overall June 12th-18th Week #6 Veg This week she's doing good after supercropping she also got topped this week. Overall she's a good looking plant no issues to report this week! Stay Growing!! ROYAL QUEEN SEEDS MIMOSA

Another week of good growth. I think. I don't like how the lower leaves are turning yellow already so early in the flowering period. I give it all she might need.

Pensavo di raccogliere questa settimana , ma controllando i tricomi con la lente di ingrandimento possiamo notare che sono ancora trasparenti, quindi non pronti per il raccolto, aspettiamo la prossima settimana e vediamo dinuovo. HAPPY GROWING!!!! Ec 1843 ph 6

Today marks week 8 of veg! For my vision with this plant I plant on letting her recover from her defoliation within the nexts 2 weeks. Then maybe a light defoliation and making the switch to 12/12. Things have been going well for this plant, as you may know she struggled at first! But now shes adapted to the nutrients well and feeding program im feeding everyother day! Water inbetween! Im also not giving the plant a lot of water making sure I do not over water the plant! Learning over here! Ive been enjoy playing and traning the plant getting her to grow big and wide and thick!!! Its been super fun! Im so ready to kick things into bloom the next couple weeks! Much love & happy growing! Lets Go GUAVA SLUSHY

Oh yes, my friends .... Peaky is back stronger than before to compete with the best! Take a look at my updates The best crops and best regards

Hi legends, Day 47 of week 7 This week has seen a massive difference, 3 of the plants have started turning purple which I find strange as all the cutting were taken from the same mother plant. we also have a leaf with seed pod the same as Bnc grower, not sure what causes this anyone? but my guess is that she just wants to get germinated any way she can.. Have started to flush with pre RO water at ph 6.3, this is near impossible to ph with a meter at 0 CF, So I have to PH the water with PH 6. 7.6 colour chart. Also this week the buds are much more resinous and the aromas are fantastic, especially fruity with a very distinct peach smell. I also noted that IMO many smaller buds have not been receiving enough light and therefore not ripening as well, these will be used for making hash looking at the trichomes most still clear but on the purple plant they are mostly cloudy. Ttfn Greenies 😍

Cómo ven va bien grande mi chemical bride y va creciendo muy bien