2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

@MephistoGenetics, Hi all the happy people here in GrowDiaries. This is my second cultivation ever and it will be fun to try a bigger space than my closet grow. First, I'm just going to say I'm done with the construction of my new growroom. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable environment in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 10 pcs 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. But there are no cultivation rooms to be displayed here, so I continue with what is most important. Today I have put my seeds in paper towel and hope the seeds have germinated within a few days. I am very excited to see how the new growroom will work and how this Stilton Special will turn out. Strain Name: Stilton Special F1 Genetic heritage - Sour Livers F3 x Northern Cheese Haze F3 Strain behaviour - Stilton grows well from the off, and develops into a stout but branchy specimine that leads to a fine yield of awesome flowers. She's not too stretchy but also is sizeable enough with good growth, to train and shape to your liking. Flowers develop pretty fast and grow to a good size, it may to advised to clear out some undergrowth and if needs be a very gentle defoliation mid-way through the grow, although leaf tucking may suffice well enough.The end product is of top shelf quality and she doesn't lack in yield either.Give your garden, nose, and body a treat and be sure to indulge in a slice of Stilton this year, You won't regret it. Size - 50 - 70 cm Structure - Medium height but bushy Flower Density - 9/10 Indica/Sativa - 65/35 Cycle Time - 65 to 70 days from sprout Yield - 90 to 140 grams as a single plant Best Method for overall high yield - 9-12 per 1,2m x 1,2m sq in 10-15 Liter pots (Soil) Aroma - Very strong, Cheesey/fruity/sour/spicey/ with a dash of coffee. Taste - Dank berries Effect - Good hybrid powerful but balanced effect Medicinal Benefits - TBA Best Grown - Indoor/Greenhouse Cannabinoids - TBA Extract information - Ideal extract candidate - High in resin, oil and terps. ................................................................................................................................................................................................................................................................................................................................................. 2017-08-21. Started to germinate the seed. ..................................................................................................................................................................................................................................................................................................................................................... 2017-08-23. Seed germinated and put in small pot in the humidity dome. ......................................................................................................................................................................................................................................................................................................................................................... 2017-08-27. Slow and steady she grows. ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-28. Transplanted to the 15 liter autopot. -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-29. New movie of the girl and a new pic of the grow room from now. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-29. Hello to you who read my diary, I just want to say that I am pleased that you have chosen to check in with me and in my diary. I just want to say that I do this for myself and for a steady flow of my medicine. Everything you read and see in my diary is 100% honest and I will never distort or beautify anything here. I document my crops so that I can learn from my mistakes and also to look back at those different crops. I try to update with pictures every day and with text if something special has happened in the garden. This is my strainhunt for the best medicine and the beginning of my journey with cannabis and the cultivation of it. ----------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-30. Cleaned the room this morning, just vacuuming and cleaning with chlorine solution. Im testing the fan to control temp and humidity, it works great. Added a movie. Everything is looking great right now. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 2017-08-31. 3 new pics. -------------------------------------------------------------------------------------------------------------------------------- 2017-09-01. New pics and a video of the room from today. ----------------------------------------------------------------------------------------------- 2017-09-02. New pic ---------------------------------------------------------------- 2017-09-03. Video of the girl this morning. ---------------------------------------------------------------------------------------------------------------------- 2017-09-04. Week 2 starts, new pics. ---------------------------------------------------------------------------------------------------- 2017-09-05. KL 08.00. Cleaned the room this morning, just vacuuming and cleaning with chlorine solution. KL 12.00. The girl got 2 liters of water with nutes in it, added a video. ---------------------------------------------------------------------------------------------------------------------- 2017-09-06. KL 08.00. The girls woke up after their beauty sleep, the leaves are always a bit down after 6 h of darkness, but they will stretch now when the HPS lamp starts. Everything looks good and I keep trying to tuck the leaves so they get the maximum amount of light where needed. Added pics and video. -------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-09-07. Day 17 from sprouting. I have never tested PH in my grows, when growing organic in soil iv learned that it adapt PH by it self. But im curious by nature so i had to test. Kl 10.00 Tested PH in the soil, it was 6.9 and tested my tap water and it was 6.8. Added video of the girl. Kl 22.30. New pics ------------------------------------------------------------------------------------------------------------------ 2017-09-08 KL 08.00. Good morning, added new video. 2017-09-08. Kl 23.50. Gave every girl 3 liters of water and nutes, added new video. -------------------------------------------------------------------------------------------------- 2017-09-09. Kl 21.00. Defoliated a lot and added pics. ------------------------------------------------------------------------------------ 2017-09-10. Kl 08.00. Last day of week 2. New video. 2017-09-10. Kl 23.00. 3 new videos. -----------------------------------------------------------------------------------

Overdrive day!! Before start i make some good picture of this girls. We will compare after 2 weeks how buds are looks like. :)

Cut the little Canuck on day 75 before dawn and placed in tub of clean water for 4 hours to remove debris. Then hung under a shady tree to dry excess water. Very dry breezy day. By evening I weigh it at 168 grams stem and all. Then hung upside down bag open to dry. It was beginning to wilt after 48 hours... hopefully long enough to break down some carbs. I’ll be checking every day an if rain threatens I’ll put in shed. Hope to trim and bag in shed after 7 days to finish drying. Pyramid is ready to cut. It will get the same treatment. I know this is a little unorthodox and a bit risky getting the plant so wet before drying. I feel like it just gives the plant an extra day to use up any nutrients and break down those starches.

This plant seems to have grown much denser and better than my first plant. I just got the ac infinity ventilation setup with controller 69 for my 2x2, still trying to learn, hopefully grow #3 goes even better. Stay tuned

Week 3 January - 9 January 6 January - feeding day

wow

Hallo mein lieber Gärtner 👩‍🌾 sehr spannende Phase die 🌸 sind mit Puderzucker bezogen riecht mittel stark. Ich hab sie gestern gegossen und hab 5 Liter gegeben und wie ihr sieht bin noch nicht so gut ausgestattet mit drain schalen und so da hat bissel wasser ins zelt ausgebreitet. Und wie ihr sieht bin mit zwei verschiedenen Dünger unterwegs der Green Hous ist sehr gut nach meiner Meinung und ich booster mit bio bizz bissel. Wie ihr sieht hab auch rote blatt stiele und ganz minimal auf äste auch

Harvest in 3 days I guess Smell is intense Very stick and delicious It’s a great work from @sweetseeds

Really cold environment but it grows really well.

Apple Fritter was doing good till air stone clogged up. Changed that and hopefully back on track. She has the polyploidy mutation. I am curious if it carried over in clone. I imagine it should. Question is should I clone the clone to keep mutation alive? She is growing in a New Level Hydro bucket, under a Spider Farmer SE5000 light. Thank you Spider Farmer, New Level Hydro, and Amsterdam Marijuana Seeds 💪❄️🌱🤜🤛 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Www.amsterdammarijuanaseeds.com SE5000 https://amzn.to/3qFpAML Spider Farmer Official Website Links: US&Worldwide: https://www.spider-farmer.com UK: https://spiderfarmer.co.uk CA: https://spiderfarmer.ca EU: https://spiderfarmer.eu AU: https://spiderfarmer.com.au Coupon Code: saveurcash (is stackable in most cases) Www.newlevelhydro.com Www.hygrozyme.com

So I’ve over watered them like a idoit and then to top it off the girl on the right all her bottom leaves went droopy and didn’t look so strong so I cut them of , also I’ve noticed some yellowing on the tips of the leaves from the girl on the left but I think that’s due to over watering , anyway I’ve done from watering everyday to when top of the coco is dry nutes are the same just gone from feeding every day to every other day but overall that’s the end of week 3 and I’m still impressed on how there looking AS ALWAYS ANY ADVICE WOULD BE AMAZING

Heute ist 3-4ter tag bei mir in erde. Wie immer top clone die sofort das licht suchen. Freu mich auf die nächsten 2-3 monate mit ihnen. Die saubersten und vitalsten stecklinge gibts wie immer bei ROOTS-FARMS. The best in austria and maybe europe

La planta en prefloracion , no ha crecido demasiado , he tenido que retirar hojas en mal estado y la planta no bebe como debería hacerlo. Vamos a ver cómo evoluciona esta semana . Dejar vuestros consejos ,gracias !!!

Week 10 of flower, looking to chop next week. The trichomes are about 70/30 cloudy/amber. Doing a few days of darkness and really cold night temps to hopefully push out the colors and resin production here towards the end.