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@PersasH
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Solo he cosechado una de las dos honey peach. A la más grande aún le faltan un par de semanas. El cogollo está muy resinoso y denso. Huele fantastico!! Citrico y dulce.
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@Brickie74
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Sour Diesel plant is growing great. Now at 27 inches and growing fast. She has been topped and is now in Flower. Now it will be watering and pruning until we get some buds.
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Unfortunately due the smell. I spray organic eco oil. Some nasty little animals to avoid. GDP stating to get some nice rainy water. All Cloudy, few orange one appear. ❤️
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After a few days I sent the plants to bloom together with others that are in the grows, I am having problems with the insects that I brought with me from the old environment where I had germinated the plants, I am trying to eradicate them but I am not succeeding , the last solution that comes to my mind is to go look for ladybugs, they are predators of these insects, I just don't know where they sell them, if I don't act immediately the plants will freeze and go into crisis!
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@Chubbs
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420Fastbuds-Greenhouse Grow 2025 The feminized strains RainbowMelon, GorillaMelon, LemonPaya, PapayaSherbet, LemonMandarin, the FastFlowering GG4/Sherbet from Fastbuds are doing amazing for there first full week in the greenhouse. The heat in SoCal is warming up with temps in the low 90's in the daytime and 60's for nighttime. Over all they're starting to reach for the stars after being topped. Besides a couple of yellow leafs I snipped at the soil level, I'm seeing growth almost daily. I'll start feeding this week Grow A & B as well as Calmag from AthenaBlendedLine. Since being transplanted into the 5gal fabric pots they've only got well water. Happy Growing.
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May 28: Excited to start some new varieties for the 2022 outdoor season! Started seed indoor May 8th under Fluence LED and transplanted outdoor to farm garden May 24th. Watered transplant and covered with plastic house to protect from wind and sun until seedlings are hardened off.
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@Naujas
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She is much prettier than she was a week ago :) I remembered that I have my first grow light, which is more compact and it will give me more space, so I changed my light, now the girl's side branches get light too:) I add a lot of video memes, because I really want to win Iphone16 pro ;) and those who don't take risks don't drink champagne:) good luck to everyone.
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Eine Knospe der Mac1 hat etwas entwickelt, das ich zuvor noch nie gesehen habe. Nach einer Recherche hat sich herausgestellt, dass sie ‚Resin Bleed‘ hat, was bedeutet, das Harz tritt aus den Knospen aus und hat sich zu einem zähen Tropfen gesammelt. Wir stehen etwa 3 Tage vor der Ernte. Ich habe heute das letzte Mal Wasser gegeben und werde sie morgen für 2 Tage im Dunkeln stehen und werde sie dann ernten.
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Woche 7 Blüte Woche 3 Die Money Maker lässt sich mit der Umstellung viel zeit. Die Wurzeln entwickeln sich auch weiterhin gut auch wenn sie nicht Weiß sind und im Eimer das Wasser auch mal knapp wird, Sie trinkt sehr viel. In dieser Woche habe ich an der Money Maker wenig gemacht außer das ich die Dünger Komponenten auf die nächste Phase der Blüte geändert habe. Voodoo, Tarantula, Piranha und Bud Ignitor habe ich abgesetzt und B-52 und Tasty Terpenes zum Dünge Schema aufgenommen. Bei Fragen einfach die Frage stellen, genauso bei Verbesserungen und Tipps für mich. Danke und viel spaß mit euren Pflanzen.
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This beautiful OG strain is such a diamond. I LOVE HER. super strong aroma, very OG, sticky flowers, very dense and compact. What else can I ask for. This strain is such a masterpiece. Definitely looking forward to grow her again. And the best is that she's 100% organic grown without any pks or any shit like that. Just Silicium flash by biotabs which contains a lot of nutrients npk and bug shit and also the soil has been prepared with Florians organic living soil. Which contains different strains of mycorrizae and endomycorryzhae. Also contains fulvic and humic acids it's such a super foof for plants that make them perform to it's full potential without adding any chemicals NPK. I only 💦 and watch the magic happen. A complete and rich in life soil is absolutely key to get a top quality organic cannabis. And here you got the results. Hope you guys enjoy ✌️💚❤️👨‍🌾
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Das wird wohl die letzte Woche, ich schaue mal wann ich ernte. Ich denke ich werde ab jetzt auch nicht mehr gießen damit die Erde etwas trocknet und die Pflanze etwas stressed. Ich werde auch 1-2 Tage vorher das Licht aus lassen. Aktuelle Werte: VPD: 1.6 Humidity: 54% Temp: 31°C DLI: 50 1055 PAR
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@DarkOG
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Boa colheita, apos algum stress algumas travaram e algumas começaram a criar seeds sem polinização no final da flora. As plantas cresceram bem suas ramas laterais formando bastantes tops. Cresceram 5 plantas diferentes, mesma strain. Na 3ª/4ª semana iniciaram a flora e duplicaram de tamanho. O engorde veio nas ultimas semanas de floração. Result: #1 = 23g #2 = 24g #3 = 14g #4 = 17g #5 = 17g Pretendo tira 20g de trimms e 10g popcorn pra extração Dry Ice.
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First week of flower , starting to stretch to the light and put in a scrog net this week after defoliating. Stretch to follow … * nearly two weeks into flower I decided to properly defoliate & it took a good 3 & 1/2 hours but my god it’ll be worth it ! Undid scrog & defoliated then placed all 5 big plants under a brand new net and really stretched them out and made sure we’re have no popcorn / larf so should be a nice easy trim once it’s all done
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@Traduhh
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Day 49: Today I changed the light schedule to 12/12. Notice some nute burn signs 3 days ago, gave plain water last feeding, Runoff was 369ppm/Ph:6.4. They seem to be very sensitive over 600ppm and start burning. Day 52: Feeding day today: 1ml Calmag, 1.5ml Silica, 5ml Micro, 2ml Gro, 5ml Bloom, 2.5ml Humic acids + a scoup of great white. Diluted to 540ppm. Ph: 6.5 / Runoff: 700ppm, Ph: 5.8. The girls are starting the stretch. Day 54: Today I did some more training with those cheap bending clips I found on aliexress. Stretch seems fast!
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Que pasa familia, vamos con la quinta semana de floración de estas Papaya sherbet feminizadas de fastbuds. Vamos al lío, de las 3 plantas, me quede con 2 por espacio, siempre pongo alguna semilla de más por si no abriese alguna por no perder ese hueco del indoor. También se trasplantaron a su maceta definitiva, en este caso de 7 litros, que el mismo trasplante provocó un shock, que también fue solucionado inmediatamente. El ph se controla en 6.2 , la temperatura la tenemos entre 20/22 grados y la humedad ronda el 50%. Las flores empezaron a formarse y progresan a buen ritmo. Hasta aquí todo, Buenos humos 💨💨💨
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@userflp
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harvesting due to the beginning of the spring equinox in the southern hemisphere and so the days have more than 12 hours of light. The plant showed signs of revegetation in recent days, the same occurred with two other plants that will make the posts and give continuity to the revegetation diaries of the plants. Att colheita realizada devido ao ínicio do equinocio de primavera no hemisferio sul e com isso os dias tem mais de 12 horas de luz. A planta apresentava sinais de revegetação nos ultimos dias, o mesmo ocorreu com outras duas plantas que realizarei as postagens e darei continuidade aos diarios de revegetação das plantas. Att 👽 ***update on effect after two weeks of healing; ironically, even though I had harvested early due to revegetation, after 2 weeks of healing the herb shows strong citrus and herbal taste and smell. about the effects ... The wave is insane, almost completely cerebral, too strong. It was a good choice to keep it in revegetation and not pick up the other copy which I also let revegetate and make a diary. Att
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Que pasa familia, aquí andamos que vamos actualizar una semana más Fumándonos un troncho de haribo con Rosin de Le Xupet Negre . Mientras tanto os comento ya en la recta final de estas Red Cookies Gelato, siendo sinceros están muy pepinas y bien resinadas, los cogollos van muy tricomados y están súper hinchados, pero comento también que la variedad no es del todo estable, es algo complicada tienes que estar pendiente de ellas cada día observar que no salga un macho porque de 100 plantas habrán salido 3 machos y alguna hermafrodita , son frágiles. Fue mi primer proyecto de cruce que aún así dentro de ser el primero a salido demasiado bien porque no esperaba ni resultados positivos pero ahí están y toda la gente que la probó, todos los que se molestaron en conocerla o interesarse por ella se agradece ,porque todos habláis muy bien de ella aún siendo así de frágil, se agradece y valora mucho vuestra opinión, Fieras. Voy hacer estrés hídrico, cada vez regaré menos cantidad para que no se mueran las plantas pero si dejen de comer de las raíces de la parte de abajo, aparte van amarilleando las hojas más rápido y mientras terminan de madurar. Hasta aquí es toda la semana y casi todo el cultivo como quien dice, así que buenos humos y hasta la semana que viene 💨💨💨
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.