Greetings from Russia to my comrades! I was busy with work for a long time and could not deal with my diaries. Now I'm free and ready to devote every day to my plants. I have a lot of plans. My mom is coming to the end of 6 weeks. She feels great and cheerful, develops well, visible progress. While I do not train him, and give him free growth. I gave my plants the enzymes, together with the chief stimulator and the main force, Then I fed the plant folic acids under the root and on the leaves. Soon I will make my girl an intimate haircut. And in a few weeks, I'll be making the third tier of the pot.

June 14th - Day 118 : Heavy defoliation today as I can’t lower my humidity and tried everything from cleaning to lowering the humid in the lung room... But I mean...!!! Looooooks at theses beautiesssss

Spargeln extrem!! Sicher 3 mal so groß wenn fertig. Als beim start

First week of adding Resin finish to stop cel devision and increase trichome production. Dropped ec to 1.35 No change on buds just yet other than mild rusting / deficiencies. Maybe light increase is trichomes. Purchased a jewellers loupe so i can now watch the trichomes ripen

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Vamos familia octava semana de floración de estás Punch Pie de RoyalQueenSeeds . Que ganas tengo de ver el final de progreso de esta variedad, las plantas están sanas, se ven con buen color. La cantidad de agua cada 48h entre riegos, quitamos todos los nutrientes de la gama Agrobeta. Y entramos en la recta final, últimas semanas de floración ya, y como se están poniendo las flores. Estas próximas semanas veremos cómo acaban. Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨.

Very easy strain to get some large yields out of. Thanks to Sweet Seeds for the chance to grow some great unique strains!

This week I started feeding my plant with an ec between 0.6-0.8. I also lowered my lamp so they get more light and I topped the plants and started lst.

Some deficiencies due to PH levels too high. Replaced with new PH pen.. Waiting on recovery, should notice difference by the end of the week.. Going to week 12.😓🎋

Hiiigh friends 🙌 Welcome to week 8. 😍 Due to too much unwanted stress, flowering is delayed. The big lady was unable to withstand the training. Her neck is broken. But she is far from giving up and will fight to the end.. 😊 See you next week. 👋 Arturo for KannaKullektiv 🙏

Day 72 10/09/24 Tuesday Feed today using De-chlorinated tap water pH 6 today only. Day 74 12/09/24 Thursday De-chlorinated tap water pH 6 today with Plagron products. Picture and video update 💚 Day 76 14/09/24 Saturday De-chlorinated tap water pH 6 with Plagron products.

Day 58 - Started on making a natural intake box Day 59 - Isolated and sealed the natural intake box + added beter sealing on grow cabin doors & cabin Day 60 - Semi-automated intake.

Started Northern Lights Automatic. I will be applying LST so it doesn’t grow too big. Been feeding it RQS Organic Easy Grow tablets. All good so far!

Things are going great. These girls are getting nice and frosty. They are so easy. And the smell! Oh she's heavenly! She's almost sickeningly sweet. I'm very excited to try this strain. Last year I was more on the Indica dominant bandwagon, since I use Kratom during the day for pain management. But my obsession with CBD Blue Shark has me excited for more balanced hybrids. I've been having an issue with the quality of Kratom I've been getting from vendors so I may have to start in on wake&bakes, which FastBuds says this strain is perfect for!

DE: In der achten Blütewoche erreicht Last Dance ein Stadium, das kaum noch in Worte zu fassen ist. Die Pflanzen wirken wie komplett überzogen von Kristallen, die Farben brechen durch, und die Terpene explodieren in einer Komplexität, die man nur bei echten High-End-Genetiken erlebt. Alles an diesen Pflanzen schreit: Finale – Premium – Elite. Phänotyp 1 – Der dunkle Funkgott Der Black-Magic-lastige Phäno läuft jetzt auf absolutem Endlevel. Die Sugar Leaves sind so dicht gefrostet, dass sie fast weiß erscheinen, während zwischen den Kelchen tiefe, dunkle Violett- und Schwarzgrün-Töne hervorkommen. Diese Farbkontraste wirken wie perfekt abgestimmt – als hätte die Genetik genau für diesen Moment designed worden. Die Buds sind jetzt maximal geschwollen: Die Kelche drücken sich übereinander, die Harzdrüsen stehen wie kleine Diamanten hervor, und die gesamte Textur wirkt dick, kompakt und schwer. Aromaentwicklung in Woche 8: Die Gassigkeit ist noch tiefer geworden, bekommt aber jetzt eine warme, dunkle Süße dazu. Noten von fermentierter Beere, altem Hash, tiefem Wald und schwerem Funk verschmelzen zu einem Terpenprofil, das wirklich nur Top-Tier-Phenos erreichen. Beim Anfassen bleiben dicke ölige Harzspuren an den Fingern – klassisches Zeichen für extrem hohe Harzproduktion und Potenz. Phänotyp 2 – Der saftige Zangria-Phantom Der fruchtigere Phäno blüht in Woche 8 optisch komplett auf. Die Pink- und Lilatöne sind jetzt viel deutlicher geworden und ziehen sich teilweise sogar in die Kelche hinein. Der Frost-Level ist so hoch, dass die Buds wirken, als wären sie von einer dünnen Glas-Schicht überzogen. Aroma in Woche 8: Das Terpenprofil ist inzwischen eine Symphonie aus: dunkler Traube, süßer Rotwein-Note, tropischen Früchten, frischer Kirsche und einer leichten Zitruswürze im Nachklang. Es ist nicht mehr „fruchtig“ – es ist komplex, schwer, dicht und riecht wie ein komplett eigener Signature-Terpenmix, der selten in dieser Form vorkommt. Beim Öffnen des Zelts mischt sich dieses Zangria-Bouquet mit dem dunklen Funk des ersten Phenos – ein absolut einzigartiges Erlebnis. Gesamteindruck Woche 8 – Der Höhepunkt vor dem Finale Jetzt in dieser Phase ist die Pflanze auf ihrem Zenit: - Buds komplett geschwollen - Farben brechen maximal durch - Trichome glasig-milchig, erste Amber-Punkte sichtbar - Aroma extrem komplex und deutlich intensiver - Harzanteil auf beeindruckendem Niveau – selbst Stängel sind sticky Die Pflanzen sehen aus wie Showcase-Material für eine Breeder-Website. Blick auf Woche 9 – Der perfekte Zeitpunkt zum Spülen In der kommenden 9. Blütewoche beginnt das Final Flush. EN: Week 8 – The Last Dance Showcase Phase By the eighth week of flowering, Last Dance enters a stage that’s almost impossible to capture with words. The plants look fully crystallized, the colors break through aggressively, and the terpene expression becomes so layered and complex that it feels like a signature masterpiece of high-end genetics. Everything about these plants says: Final stretch – premium – elite cut potential. Pheno 1 – The Dark Funk Titan This Black-Magic-leaning phenotype is now performing at absolute top-tier level. The sugar leaves are so heavily frosted they appear nearly white, while deep violet and dark forest-green tones push through the calyxes. The contrast is insane – almost cinematic – as if the strain was bred exactly for this late-flower moment. The buds are now at maximum swell: Calyxes stack aggressively, trichomes stand tall and dense like crystals, the entire flower mass looks heavy, tight, and fully matured. Aroma development in Week 8: The gassy core has become darker and richer, now wrapped in warm, heavy sweetness. Notes of fermented berry, old-school hashish, deep forest funk, and that unmistakable dark Black Magic spiciness fuse together into a terpene profile only elite phenos deliver. Touching the buds leaves thick, oily resin streaks on your fingers — a classic indicator of extremely high potency and mature resin glands. Pheno 2 – The Juicy Zangria Phantom The fruit-forward phenotype hits full visual bloom in Week 8. Pink and purple hues become more pronounced, bleeding slightly into the calyx tips. The frost level is so extreme the buds look like they’re coated in a thin sheet of glass. Aroma in Week 8: This terpene profile is now a full symphony of: dark grape, sweet red wine, tropical berry, fresh cherry, and a citrusy sparkle in the finish. It’s not “fruity” anymore — it’s deep, layered, complex, and genuinely rare in its structure. When you open the tent, the Zangria bouquet blends with the dark funk of Pheno 1, creating an aroma that feels completely unique and unmistakable. Overall Impression Week 8 – The Peak Before the Finale At this stage, the plant is on its absolute zenith: - Buds fully swollen - Colors breaking through in maximum intensity - Trichomes milky with scattered amber - Aroma dramatically intensified and highly complex - Resin production at showcase level — even stems feel sticky The flowers look like something straight from a breeder’s promotional photoshoot. Looking Ahead to Week 9 – The Final Flush In the upcoming 9th week, you begin the final flush. This stage ensures: a clean fade of excess nutrients, boosted terpene purity and clarity, smooth, clean-burning ash, and the highest possible quality for your final product. During the flush week, colors often deepen even further, buds harden up, and the aroma becomes sharper, more defined, and unmistakably finished.

06/03/19 - unfortunately this was my own fault for being gullible with a mix of just not thinking, but I got a cal deficiency on my OG which was fitting seeing as everything was going well, but I think I have resolved the problem as I’m not see the new growth being affected but I do think it has slowed down in growth abit but I’m guessing that’s understandable, I’m really happy I’ve turned to mills as I can tell a difference from my previous grows, this slight hiccup was my fault but they were that healthy I’m almost positive it’s gonna spring right back 🤞🏻 09/03/19 - definitely looks like it stopped growing for awhile as the gelatos are a hell of a lot bigger now compare to my OG but I think the problems been solved as their was only a little progression on some of the newer leaves but the newest set of leaves seem unharmed includeding the side branches. 12/03/19 - a lot smaller than the gelatos but never the less I’ve started the LST/leaf braiding with the OG aswell to make sure the side branches catch up as they were stunted from the cal mag def

La banana è un sweet chesse (che sono entrambe nel vaso airport) in particolare la Banana sono in carenza di magnesio. Ho aumentano top max vedendo di risolvere il problema. Le altre due piante sono nel vaso air pot Ercole.

So I finally have time to finish this review. The buds cured now for over a month and I consumed them a lot because I simply don't want to smoke something else. The details are in the strain review but to make it short: 10/10 high, 9/10 taste, 0/10 grow (she hermed).