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Good day everyone, 3 days ago I harvested the Pineapple express. I can say only one - this was VERY fast grow. The fastest one I had ever. So I know why - Fast buds genetic DWC simple system I can not say anything about the strain yet, but the aroma is amazingly sweet and fruity. The buds are fully covered with the white crystals. I will update the DRY bud weight within 1 week the smoke report. Good luck

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Почки набухли как лицо астматика дяди Жоры, после того, как он покурит сушеных листьев клёна. Это критический прогресс в созревании. Выглядит многообещающе. На следующей неделе делаем Ripen и потираем руки в предчувствии скорого урожая. Но у меня с терпением всё в порядке и я хочу сделать эти цветы с максимально критическим эффектом. Я хочу назвать её "пол банки" или "курить лёжа" или "билет на Марс - раньше Илона". Запах сладкий, душистый, луговые цветы. Опустил лампу на 10 сантиметров. Мощность лампы - 100%, от растения 50 сантиметров, 55000lux, лампа теплая. https://www.viparspectra.com/products/xs-series-xs2000 при покупке примени код и получи скидку 😀👌 код: Itxsgdvip Тех, кто посмотрит все фото, в конце Вас ждет видео! Спасибо всем за поддержку и комментарии! 👊😁 Ищу спонсоров и предложения, для всех выгодное движение! 😀👌

Привет садоводы ! пару дней был в отъезде и за эти дни соцветия стали тяжелыми , некоторые ветки завалились на бок придется делать им сцепку из проволоки это только пятая неделя сегодня я полил растение обычной водой и наверное в следующий раз переведу ее на RIPEN А в общем отличный цветок растет Сегодня я полил ее Ripen

Day 57 and looking forward to harvesting. Hoping to dry slowly. Gotta chop with others all at same time, so just waiting on this girl to wrap up. What do you think - she about ready for harvesting? Harvest day 60 Found seeds, nanners and pollen sack - fucking lame. we grow again another day! Likely will make bubble out of this stuff.

We're halfway through the first week, day 10 to be more precise at the time of the photo. We've chosen to stay on 24/7 for at least the first few weeks, as we're in a hurry to finish at least the autoflowering plants so we can have some weed for Christmas. Our Karen Kush Auto is the best of the bunch, the queen of our autoflowering plants right now. Anything can happen, but look at the difference compared to the others. It seems incredible that she did all this in 10 days, but it happened. This is thanks to the 24/7 light, a practice that in my opinion is a bit aggressive, but one that growers in a hurry to harvest absolutely must embrace. I'm now going to switch to 20/4, but many are leaving it until the end. Naturally, it changes the nutrient intake a bit, and there are some delicate aspects to be careful about. The chosen soil is the recently released Plagron Bio LightMix, which will allow us a more regular and reliable start without the sudden changes that liquid fertilizers can cause. We've started fertilizing for vegetative growth: - Pure Zym 1 ml/l - Power Roots 1 ml/l - Sugar Royal 1 ml/l - Alga Grow 1 ml/l I calculated my personalized data sheet directly on the Plagron website. If you have the organic light mix, which isn't available yet, download the organic one; the values ​​are the same. www.plagron.com This seedling started out very well; the other had trouble releasing the seed and placenta, and germination stalled. I'm germinating another one; it's arriving very soon. https://www.zamnesia.io/en/10783-zamnesia-seeds-karen-kush-auto.html Zamnesia Description - There's no need to complain about this strain: Karen Kush Auto will satisfy even the most demanding smokers with its dense flowers, fragrant terpenes, and high THC content. Created by crossing Monster Bud Kush, White Widow, and ruderalis, Karen Kush Auto boasts first-class genetics that leave no room for complaint. Inhale its sweet, spicy, and diesel aroma and enjoy the physical buzz that envelops you. After a few minutes, the only thing you'll want is a comfortable place to relax. And best of all, you won't find this strain anywhere else, as it was created by the geneticists at Zamnesia Seeds. www.zamnesia.com

These girls are tall already. I can't wait to see what's next!!! No issues. Thanks for following!

Hi growmies, quick update this week nothing special to report, no news good news, see ya next week

Week 1 of early Veg I began the feeding schedule this week using the Canna range again for this grow as it worked well on my last run. Trying to maintain 55-60 Rh this week Growing an Auto strain for the first time under the new LED is throwing up some questions but I think all is going ok. These 2 seemed to take quite a long time to settle but are picking up growth now and look to be healthy. I plan to lower the light gradually over the next week to 24inch as they develop further. 🤓 ✌️

Licht Täglich erhöht bis auf 70% (Zu viel Licht)

This week has been going good I think I will be chopping some of the buds off of her within the next week to start the drying and curing process im going to try my hand at cloning this lady.

Booommm! Llegó la hora tan esperada Farmers nuestras flores llenas de resina acabandose de formar, la verdad que estas genéticas ayudan mucho al desarollo del cultivo espero que os guste!! Una locura de olores y terpenos!!💚

I finally got them in the garden! It was a trip getting out there, so they don't look the greatest because they're stressed. I made a video explaining almost everything except I forgot to mention that I added about 5-10% Biochar in with the Fox farm potting soil. Also, the row cover will protect against pests.