Hello friends here we are in week seven of flower. Sorry there was no daily time lapse or pics went out on vacation. So today Day 105 12/24/2021 marks the end of the week, She is coming along nicely and the smell is just WOW. She should be ready to start flush after next week. I checked the Trichomes and mostly all cloudy few clear and few amber. Also got a new tent last night that will be setting up for next year grow.

The frostiest plant I have ever grown, I will enjoy watching my growmies do the same. Buy this seed and share it. It is a wonderful cultivar. I had two disteonct phenos, both absolutely gorgeous and unique

Everything is not as accurate as I would like to say, but I wanted you guys to see what I can do

She flowered very fast. The smaller ones smell different and have more shiny flowers very nice, it smelled very strong at the end, while trimming the buds a smell of fruit and gas spread trough the whole house

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

MrJones Ztrawberriez Auto 🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓 💧 Feeding - Using Gaia Green Grow / Bloom and Compost Teas 🍃Training / LST-Defoliation-Topping TBA 🕷️ IPM - Will be using Green Cleaner" 1 OZ per Gallon, and CannControl from Mammoth alternating between products each month for Integrated Pest 🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓 ▶️Sunday 10.29.23 - Going to leave the light cycle alone for another week, these ladies are blowing up pretty well, don't want to mess with the MoJo :) 🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓 ▶️Bud Description Ztrawberriez Auto produces long and fat buds with a beautiful light green shade with oftentimes purplish hues and a thick layer of resin that starts at the top of the flowers and continues all the way to the tip of the sugar leaves. It’s the typical bud structure you would expect from hybrid varieties topped with an explosion of fruity terps that’ll hit you in the face with a blend of creamy, fruity, and sweet aromas as soon as you break the buds open. Definitely a must for terp heads and extractors looking for exaggerated flavors and aromas 🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓 ▶️SOIL MIX 40% ProMix HP 40% Coco Coir 70/30 15% Worm Castings 05% Malibu Compost 🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓╰⊰🍓´🍓⊱╮🍓

Plants are doing well, The 84" inch tent is a must if your not training or topping. I removed all the sacks that appeared last week and I haven't seen anymore show up so fingers cross, I check daily. Bigger tent also helps with heat control as the air has more room to rise. Hope you all like the pictures, I started to take videos as they provide more detail with less work.

Week 5 of flower is over! This grow is going by so fast because I'm so busy with so many other things I feel like I haven't really paid much attention to these girls but they are doing so well. Bulking up heavy now. Smell is amazing. Very dense buds. Can't wait to try this one out. Few weeks left now.

Läuft wie von alleine

📆 Semana 6 La Variegated Sour GPP está avanzando con personalidad propia. Aunque no ha mostrado variegación en esta ocasión, se está expresando de forma muy peculiar: los cogollos tienen una estructura apretada y densa, con una forma que recuerda a una pequeña coliflor —compacta, nudosa y muy distinta al resto del jardín. El plan nutricional sigue siendo el de XpertNutrients, que está funcionando sin pegas. Esta planta está comiendo bien y sin quejarse. El ritmo de riego lo mantengo ajustado a su consumo real, evitando saturaciones y permitiendo que respire entre riegos. Los Adlite siguen haciendo un trabajo excelente, y se nota sobre todo en cómo ha desarrollado las puntas: la iluminación pareja ha ayudado a que los cogollos se formen de manera simétrica y con buena producción de resina. A nivel climático, seguimos con temperaturas en torno a los 22-25°C y una humedad del 55% que, aunque no es ideal, no está generando problemas gracias a una buena ventilación y movimiento de aire constante. Los aromas comienzan a destacar con una mezcla ácida y ligeramente especiada, fiel al perfil Sour. Los tricomas se están volviendo lechosos, sin señales de ámbar todavía, así que aún queda margen para que siga cogiendo cuerpo. No tiene la variegación que esperábamos, pero sí una expresión floral única que la convierte en una candidata interesante para seguir observando. ¡Seguimos creciendo fuerte! 💪

Now tent is here ! plants are exploding:) little one starts to catch up ! Trust in the process👍🏽

Harvest day! Whole plant gets chopped and hung First day of of dry 10/2/25 Day 4 of dry 10/6/25 buds are drying up not much chlorophyll smell left except for on the bigger fan leaves that I kept on the plant buds are giving off a nice pungent smell

Hot, dry, couldn't of asked for better weather all week. 10g airpot started to flower, earlier then last year, should be excellent finishing weather. Super crop abuse from last week is recovering, height hasn't moved which is great, she'll be stackin in no time. Canopy on the large one is developing nicely, super flat. I pulled the net out which helps to gently guide and redirect the canopy as it gets to max height, and adds some epic support in the later season when size and winds are a factor. Just a shot of Monster Maxx, silica, 90min drip and weekly LCPT spray this week, smooth sailing.

She has been competing with other plants for light. That's why she is so stretched.

August 10-15th Flowers were popping and the tent looked happy and healthy

And another week has passed. The girls are doing great. --------------------------------------------------------------------------------------------------- Controller AC Infinity Pro - connector for the external light control, RJ-12 - 2 plugs with which I can control dehumidifier and humidifier. - 2 fans run 24/7, 1 Oscillating from spider farmer Light - 12/12 h PPFD - 900-1000 nmol VPD - 1,3 - 1,6

It's been cloudy for days but no rain right go figure ..have to try and keep those 2 cannabis off grid.. Experiment keep moving it for sun.. Then neighbor informer lol.. Im playing spy behind enemy lines ( right next door )..lol lol. Dam snitch..why other people has to watch other people is my guess

Ph issues resolved end of Week 10 with multiple applications of lime and 2 flushes with PHd water at 7.5 Just waiting to chop the El Chemi Kiwi at this point which is very close and most likely can be chopped right now. My drying tent is currently in use as I wait the weather to turn around so I can harden them off and plant in the back yard. The strawberry nuggets is likely 1.5 weeks away at least but it is really fattening up more than the chemkiwi.

Hi guys, The plant is done stretching but am showing N deficiency in lower leaves. I don't know if its because of the light mover, the lower plant not getting light, or maybe its the genetics. But should I be concerned? I tried to add some blood meal in my weekly tea and an extra dose of macro nutrients just for this one plant, but still seeing deficiencies. Well this is why I like to grow these girls, they behave differently.