The Afghani x Mac1 auto from DNA recovered well from the training and is in full bloom. Bud sites are looking great. Had an issue with humidity this week and the leaves started looking rough. She got a dose of Recharge this week and drinks about 8-12oz of water throughout each day. All in all she's trucking right along. Very happy with how smooth this solo cup grow is going.

1 DE AGOSTO - DIA 8 ✅💨 - Hoy puse en funcionamiento el ventilador para remover el calor concentrado de la lámpara por sobre las plantas y distribuir aire fresco en todo el indoor. Beneficiando también a el tronco y las ramas, generando una resistencia al viento. El crecimiento se ve uniforme en todas las plantas. 2 DE AGOSTO - DIA 9 🌱💪🏼 - Hoy es un gran día!! Apliqué la técnica de LST a todas mis nenas. Mi deseo es obtener buenos resultados al final de la cosecha, pero ya me siento muy feliz porque al final del cultivo lograré ganar experiencia!! 3 DE AGOSTO - DIA 10 💦👨‍🌾🏻 - Hoy es día de riego. 1 litro de agua para cada una de las niñas (600 cc desclorada y 400 cc destilada) - PH 5.9 📊 - LSD-25 #2 y SIX SHOOTER #1 parecen ser las más vigorosas del cultivo. - Relleno las macetas con tierra debido al crecimiento del tallo de las plantas. Todo marcha muy bien!!! - LSD-25 #3 aparentemente esta pasando por un leve estrés, ha dejado de crecer a la par de las demás. ¿Que puedo hacer al respecto? ¿Perjudicará el ciclo de vida de la planta? 4 DE AGOSTO - DIA 11 💡📏 - Subo la lámpara a 40 cm de distancia. - Por lo visto las niñas respondieron bien a la técnica de LST. 5 DE AGOSTO - DIA 12 - Reviso la humedad del sustrato y aparentemente se encuentra en buenas condiciones, por lo tanto mañana voy regar las niñas. - Como todos los días controlo los ganchos y la soga colocadas, parece que las plantas se adaptaron al entrenamiento sin ningun problema. LSD-25 #2 y SIX SHOOTER #1 tienen un crecimiento mas rápido por sobre las demás. - LSD-25 #3 que habia pasado por leve estrés (dentro de mi punto de vista) parece comenzar a desarrollarse de manera normal. 6 DE AGOSTO - DIA 13 💦👨‍🌾🏻 - Hoy es día de riego. LSD-25 #2 y SIX SHOOTER #1. En primera instancia regaré solo a ellas dos, ya que el sustrato se encuentra bastante seco. 1 litro de agua a cada una (500 cc destilada y 500 cc desclorada) con una dosis de 2 ml VOODO JUICE 💀 y 2 ml PIRANAHA 🐟 - PH 5.9 📊 - Antes de finalizar el día repetí el riego con todas las demás nenas (LSD-25 #1, #2, SIX SHOOTER #2 y GORILLA GLUE) 7 DE AGOSTO - DIA 14 🌱💪🏼 - Hoy cumplen dos semanas en tierra. Estoy muy contento por que se nota el progreso de todas!! Cargue las fotos del día para culminar esta semana, se puede observar como el tallo y las ramas se van adaptando a los alambres que les fui colocando. Una BELLEZA 😍

2024-07-18 INDOOR Final Week for the Tangerine Snow. Shes fading wonderful, and pistils are changed. Trichomes are very good, so in a few das she will beHarvested. OUTDOOR Shes doing well, shes in stretch and i can see first d´signs for going into Flower too. She got a Feeding, to stay Happy Temperatures are hot and its sunny, so everything good for her finally Summer has come. BREEDER INFO Tangerine Snow F1 Fast Feminised is a 75% sativa, four-way cross of (Boost x Tangelo) with (Lavender x Power Plant). This Fast F1 hybrid is bred from Cali genetics and boasts great citrus terps, high resin production for extracts, high levels of THC, very good yields and excellent mould resistance. Tangerine Snow F1 Fast can be grown indoors as well as outdoors. Indoor flowering times are between 8 - 10 weeks while harvest time in northern latitudes is during September while in the southern hemisphere growers will be harvesting during March. Recommended climate regions are hot, dry, humid and warm. These are tall, semi-branched plants that grow in excess of 200cm and display a high degree of vigour with very good uniformity. In common with many other heavily sativa-dominant strains, Tangerine Snow F1 Fast offers excellent resistance to mould as well as to plant pests and diseases. The combination of citrus terps and plenty of resin makes thi a very good extract strain with the 'washing' method delivering very good yields of hash. The citrus terpene profile is reminiscent of mandarins and tangerines and also has sweet candy notes. THC production has been lab-verified at a strong 24% while CBD is low. The effect is uplifting and energising, perfect for use during the day and early evening.

Are these autoflowers doing ok my first grow week 4

Day 13 of flowering : we are starting to see the pompoms appear 🏵️🏵️🏵️

Flushing

Merry Christmas growers!!! Been a very good week, had plenty of time with the ladies. Nothing major to report other than these two LED's aren't enough for this size tent I don't think. Will definitely be upgrading for the next grow! Thursday - fed roughly 1.5L with nutrients. Friday - did nothing. Saturday - fed roughly 1.5L with nutrients. Sunday - did nothing. Monday - fed roughly 1.5L with nutrients. Tuesday - did nothing.

~ FastBuds PAPAYA SHERBET Photoperiod~ Well here we go on another journey together through 'Canna-land' and this one's kinda special... forget that, it's VERY special because this is FastBuds newest surprise for the community, a FEMINIZED PHOTOPERIOD STRAIN!🤯 I was fortunate enough to score these seeds, of which only 1000 were available and can't wait to see what this strain can do! This Papaya Sherbet, according to FastBuds, is a hybrid with a 9-10 weeks flowering period. For a more detailed and accurate description of this strain the following from FastBuds says it best: "Combining the massive stature of Papaya (Oni Selection) with the strength and resilience of one of our best keeper cuts (Sunset Sherbet), this strain develops into a big, expansive bush adorned with numerous bud sites that later transform into a generous harvest of medium-sized buds. Papaya Sherbet flowers deliver a signature flavor of premium cannabis with subtle citrus undertones that emerge upon inhaling. During growth, her aroma makes for a sweet yet pleasantly bitter fragrance, giving you an idea of what the smoke will taste like. Notably stress-resistant, Papaya Sherbet is a great choice for growers working in challenging environments. She is very forgiving and rebounds quickly from any adversity, allowing growers the freedom to experiment with confidence that she will take everything like a champion she is. This strain embodies resilience, flavor, and abundant yields in every grow cycle." Sounds like an epic strain and I personally cannot wait for this lady to strut her stuff!😍 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ The Setup: This is going to be an outdoor grow, but I have started the Papaya Sherbet photoperiod indoors as our weather is still a bit too chilly to put a newly sprouted seedling outside (nighttime temp's dipping regularly into the 40's℉). The plan is simple... let her grow inside under a 19/5 light schedule until the nighttime temperatures are in the mid 50's℉, which shouldn't be long. After which, she'll be moved outside and transplanted into the soil which I have already setup and inoculated with beneficial microbes from BioTabs and slow release dry amendments from Gaia Green. Once she's established herself outside she'll be given periodic top dressings of Gaia Green 4-4-4 and 2-8-4 along with worm castings and Compost Tea's. Her grow area is approx. 5'x5' and I have posts and a trellis net set up already for when she gets bigger to aid in training her. Let the fun begin!🤪💚 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 6/16- Week Six is here and the Papaya Sherbet from FastBuds is beginning to finally take off! Our weather is beautiful now with daytime high temp's in the 80's F without a lot of rain so I am watering her daily at sundown with approximately 5-7g of well water. 6/18- I gave the Papaya Sherbet photoperiod a good watering today at sundown via the garden hose with straight well water. 6/20- I watered today and also did a little canopy maintenance, removing some leaves down low, along with some small shoots that would only sap energy better used elsewhere on the plant in the future. 6/22- Six weeks from seed and I have a sneaking suspicion that the FastBuds PApaya Sherbet photoperiod is getting ready to take off in a big way! I could be wrong, but we'll have to wait and see! Thank you for checking out my diary, your positive comments and support make it all worthwhile! 💚Growers Love!💚😎🙏

Ready

April 18: Thank you MSNL seeds for the package i germinated as soon they got here 😄 So i start with 2 strains in my small tent, then when the 4x4 is free i will pump it full too with all of these for a few crops, cheers April 23: they both above ground now April 20: the seeds have roots and are now planted in 2 liter pots

150-200PPFD 20/4 Mein Grow Stuff Messen : + Messgerät - X-Sense WLAN Hygrometer 1 Basis SBS50 + 4 Sensoren + + Basis + + 4x WLAN Sensoren RH und Temp + Amtra Digital PH Tester + Amtra Digital TDC&EC Tester + Photone App (android Samsung S21 5G werkscallibriert (geprüft mit lichtreferenz)) + GEHE ph-Wert 500 Testkit + + Testflüssigkeit + + Pimpette + + Probenröhrchen + + Propfen + Pimpette 10ml Zelt : + Keimung und Vegetative Phase + + Growbox - DiamondBox Silver Line SL60 (60x60x160cm) (hohe luftfeuchtigkeit) + Blütenphase + + Spider Farmer 70x140x200cm Indoor Grow Tent Licht : + SpiderFarmer SF1000 (100Watt) Dim (hohe luftfeuchtigkeit) (Vegetativ) + Sanlight EVO 1.5 3-60 mit Bluethoot Dimmer (niedrige luftfeuchtigkeit) (Blüte) Abluft / Filter / Rohr: + 2x Abluftset - PrimaKlima PKEC-125-TC Set + + PKEC-125-TC Abluft + + PrimaKlima Aktivkohlefilter K2601 Flat (bis 70%rH) + Staubschutz + + Luftschlauch Aluflexrohr (Ummantelt), 3 Meter, ø 127mm + + Verbindungschelle 50mm (Metall mit Schaumstoff mit Aufhängemöglichkeit) + + 1x Universal-Schlauchschelle, 60-165mm Samen : + 3x Royal Dwarf Automatic (RQS) (buy from Zamnesia Seeds) Töpfe : + Nothers GZjiyu 6 Stk Pflanzsack Pack 2 Galonen = 8 Liter Stofftöpfe Medium : + BioBiz LightMix + + Urgeisteinsmehl für Ergänzungsstoffe + Vulkangranulat (Aquabedarf , drenage) Ventilator : + pro Zelt + + 2x Ventilator - Spider Farmer Grow Ventilator (EC 10Watt) PH Korrektur: + 500ml Plagron PH- + 1000ml Canna PH+ Dünger : + Dünger - Canna Terra Kit + + 1000 ml Terra Vega + + 1000 ml Terra Flores + + 500 ml Rhizotonic + + 500 ml PK13/14 (wird nicht genutzt , da unnötig) + + 1000 ml Cal-Mag Agent (fals ich den mal brauche) Entfeuchter : + Entfeuchter - MeacoDry ABC 10Liter Zubehör : + 1L Nanoprotect Isopropanol 99,9% (Reinigungsmittel) + 1x Topfuntersetzer 30x30cm + Grießkanne 5 Liter + Pflanzendrath 1mm x 5m + Sprüflasche 0.7L + Drucksprühflasche 2L + Messbecher + + 1L + + 0.5L + + 0.25L + Eimer 10L + Gartengießkanne + + 5L + + 10L + Plastikabdeckung mit löchern fürs Sämlingstadium (ehemals Kuchenabdeckung) + Luftschlauch Aluflexrohr, 3 Meter, ø 127mm + GrowPRO Schere Curved + Gardo Gartenschere GDGSB 180 + GrowPRO Pflanzendraht für Low Stress Training, 5 mm, 4,9 Meter + Dosierspritze, 5ml + 2x Universal-Schlauchschelle, 60-165mm + LIMITADA 15 Stücke Pflanzenbieger Verstellbar + 6er Steckdose + 2x Zeitschaltuhr (mechanisch) + Müllsack (umbau zur Trockenkammer) + Pappe + 2 x Gafa-Tape (Rolle) + Klettband 3m + Airpump Kit + + Air-Pump + + Luftschlauch silicon + + Air-Stone Trychome analyse : + Samsung S21 5G optical Zoom + DIGIMICRO Mobil + + x300 optical zoom + + HD Video + + 12MP photo + GrowPRO Mikroskop 40x mit LED CureBox : + CASO VacuBoxx E-Set Vakuumbehälter-Set aus Borosilikatglas mit Tritan Deckel + + 145x60x105 + + 170x65x125 + + 200x80x150 + + 220x80x165 (about 40-50g dry buds space) + + manual vacuumpumpe + + adapter für automatische vaccumpumpen VideoSystem: + GoPro Hero3+ Black + + Zeitraffer : 1 Bild pro Minute , gerendert in H.265 60fps . + + 12MP Bilder + + 1 Sekunde Film entspricht 1 Stunde Real . + + Ohne Dunkelzeiten. + Bild/Videoprogramme + + Photoshop (Bildsequenze render Video, Ausschnitt und Beschriftung) + + XMedia Recode (H.265 Neucodierung)

Bellissime,abbondanti e cariche di infiorescenze resinose e profumate! Dimensioni grandi e qualità altissima! È stata una settimana bella e calda e ormai siamo in estate quindi continuano a riempirsi di infiorescenze molto resinose e dure. Ogni varietà è una vera soddisfazione! Grazie a tutte le banche dei semi che hanno creato queste varietà magnifiche! Per alcune manca poco alla raccolta mentre altre ci vuole più di una settimana di attesa anche due settimane. Sono felice, bravissimi tutti.

Week 15. Had a look at the trichomes on the a few sites. 50%cloudy some embers rest are clear. And getting very long in comparison with her sister zkittlez organic. This bud seems to be hitting that 21% THC better, yet, lots of things are still an unknown. For example the exact THC/YIELD /QUALITY. Finished with the grow nute, to see can I bring more resin/trichomes. Seems to have been ok. Won't know it's true effects if any for a wk or so. Moved back on to bloom and bloom nutes, root stims, enzymes. Gave a hard flush to get rid of any old grow left and clear the weeks salts. 31/3/22 Led grow bars arrived. They sent me the wrong1s. So contacted them and they said instead of sending the 4 bars I ordered they sent this set that is better than the 4 and also gave me back my all the money for that part of the order and gave me 40e light bars for free. 31/3/22 Installed both 48x2 led chip bars 10 both bars are 5 watts each. Should help increase bottom-mid growth as it lags behind. The SF-1000 Light is great this is just for the rest of the huge zkittlez to catch up. RH is in a good range today 54%RH for the whole day since I woke up. Slowing down on fluid uptake. Use to be over 2l before she'd throw anything back. 1.8l is her limit now. I know I'm abit all over the place. But after looking through my nutes. I missed out on giving Nirvana, yeah the soil grows got it once a week but in coco meant to be from wk3-6 of bloom currently we are in week 6, just started so gonna squeeze in a weeks worth of Nirvana and its increased the PPM by 250 aswel as the. 5 increase in some PK focused nutes and some extra silica for the plant as she is weak and has HST'ed 3 colas and 2 stems. So, gonna try put her back on it. I really have been all over the place with giving nutes, more of a case of tweaking things to figure out what's right. After my beginners buys I can now say I can find the same if not better nutes that do the very same thing in the AN line or the GH tripack. They also have kleen roots that's like voodoo juice. Must cheaper, and then we have senzyme and ancient organic earth tea for piranha and tarantula which cost 70e a L as opposed to 12e a L for senzyme and ancient tea. It's just a case of shopping round and not getting sucked in my big words. Check out diaries that these fungi/enzymes are used and they work just as good as the expensive stuff. Also the jungle juice micro is a great trace element nute. 2/4/22 Stacking on lots of nice looking terps colors of purple in the bud. With lots of sugar balls. Hopefully buds will get bigger by about 10-20% hard to see but still about 15-20 white hairs. Much more lower down the bud it's it's like 50/50 stigmaVSambers Decided for the plants benefit I'm doing a 30min hard flush and restarting the whole nute programme again. Just got a little run-off back from a 1.8l feed and was 2200ppm 800 nearly more than the feed. 2/4/22 Did a hard flush today. Took 3 mins to get it down from 2200 to tap water level then threw 500ml of filtered water with cal mag extra 1ml/l. She was so sticky just now after lifting moving her colas with my arm to the side to take the TDA pic. Fuck she is so sticky. I took the HST-ed bud today. Weighted in at 1.9 wet after trim. Needed a sample anyways. It's looking like I'm going to have to do a 2 fucking part harvest. As there is just waaaayyyy too much undeveloped bud sites to harvest the plant I'd be losing over 1/2 of my harvest. So I've decided when the top 5 nodes are done. I'm gonna take them and and set the plant up evenly and give her another 2-3 wks to finish and I should get way more if I bring her to harvest in total in 2-3wks. I'd be losing soooo much. It's hard to show you guys what I mean. All you can see is the top bud sites. But that's only 30% of the plant. At least 70% is behind 50 of that is so far behind it will not catch up in time as long as these top 5 colas are taking all the energy and nutes, it's the only thing that makes sense at this stage. With the added lights I know there only 10 watts but I'm hoping that will increase my mid sites. I'm gonna just try take as little off as I can but max on what I do take off should hopefully bring her about and I'll be for sure giving the rest of her a 3 day rest period in the dark. Oh btw. If anyone has any idea as how to go about this message me. I've read up all I can. I NEED PPL WHO HAVE DONE THIS BEFORE. So heres my plan. I'm gonna lower down the wk before I take the colas back down to a 16-8 then I'm gonna let her rest for 24hrs with full light water and so on, (she'll be on flawless finish for the week), hoping that'll keep what I need alive to finish her off, cause if she dies on me, I'm fucked. I know 24hrs without light is not good. But 50/50 she'll pull through and go right back to work after I take her colas off I'll hard flush her and back on nutes for 2-3weeks. What u guys think? 4/4/22 GREAT UPDATE...I'M SO FUCKING HAPPY 😁😁😁😁 Finally, some bud anyone would be proud to have produced. It wasn't even fully done, the bud I dried in a box took 4 days. Got .45 back from 2.1 it was fucking AMAZING!! The bud isn't even done, wasn't rested. Cured and the taste/stone is top class. That alone I had a joint and a bong. And I'm BAKED. Defo more leaning on the indica side 75/25 Sativa. But left decent amount of trichs in the 3tier grinder. Defo needs a flush and to be finished she's going to be pushing near 20%THC. She already nearly there tbh but that bud alone had about 40% clear trichomes. And she's still more indica than sativa. The organic bud is 12-15% the 100 sativa. This bud, bloodshot eyes, heavy head stone. Munchies galore. I'm stuck to the couch. I WANT MORE. Roll on baby roll on, I'm so tempted to chop her. Not gonna happen though. I'm over the moon though. TBH i was worried that this bud would be 12-15%. Thank God it's not the case. It's powerful. Doesn't take much to get high. Idk about you but if I don't get that from bud within am hour I'd go through 1.5-2gs by myself to get what. 5 gets you in 10mins. I've slowly started to feel the Sativa high, uplifted, really happy after about 5mins of smoking my joint/Bong. Then 10-15 mins later bye bye sativa and HELLO indica. Heavy head high the last 2 hours 30-40mins in I got the munchies heavy. KFC sorted that, I dunno about you, but a weed is like a nice glass of wine and the weed made my meal go down a treat. Will be running it off for the next week but it was worth it. Think I'll have myself q little sleep now, in a little goof 2 hours in. 100% NOT A DAY TIME SMOKE. I can hardly operate this phone and I'm having trouble remembering what I'm writing, oh and am I raml8ng. 🤣🤣 night night at 6pm. I also took a 2.5 gram tester, it was a bud that I k8nds broke off when checking for stigma' (white pistils underneath the bud at the top part of the top cola broke off and this bud has purple notes in it, it's the very same looking bud that is in yday picks, That's when I broke a 2.4 wet purple resin/trich balls bud. It glitters in the light looks fill of trich balls. She's 75 cloudy. Some milky turning cloudy and about 10% long clear tops with milky bottoms, but the bud was taken by mistake just as the light turned off so it had no rest period at all. Hopefully if I can keep the RH low I'll be able to sort out my plants and even make them stronger over the next few weeks. BTW, For coco, taratula&piranha are very expensive, yea they have some benefit in coco of course. But there is much cheaper alternatives in the AN line. Senzyme will help break down old roots like tarantula, help nute uptake and also has piranha properties. Also mixed with ancient earth organic tea and that is more than enough fungi/enzymes for coco. But if you don't mind spending the money, you can use them but they are best in soil. Even do a 50/50 soil light mix/coco that alone is the best recommend substrate for doing autos/fems, especially if you are wanting to make the most out of these expensive nutes. Voodoo ((Tarantula)) ((Piranha ))) Use these cheaper subs for coco with the voodoo juice. SENZYME+ACIENT EARTH ORGANIC TEA. 6/4/22 Okay, so things, not so well. She's stopped taking nutes. And cut drinking by a 1/10. No nute change from the feed. She's in nute lock. It's cause the top parts are done. And they are the main colas but everything else coming from them is miles behind. So, my minds made up. I'm going to top harvest this week. I've no choice. She isn't taking nutes. If I don't do it now the bud/larf below will mature into light airy bud....larf. gotta get her back taking nutes. 6/4/22 YAY, Finally a dehumidifier that works. This thing literally has been running 1 hr. With the grow room door being closed the RH can drop to 45% but with the HEPA filter the smell is gone out the house and it has heated the whole house, it's crazy what this 20l dehuimifier does. Bonkers. Finally I am hitting peak conditions for growing, when my door is closed I can get the RH in the tent to hit 70%+ and now with this machine I can get it as low as 45%, it's only been on 1hr. In my tent this is how much electric it uses. Look at the pic above 218watts in 20c temp 60% RH that's our house when the heating is off by turning it on it drops in the range of 50% after a couple of hours. temp is 23% so I'd say it uses in the mid range so maybe 230watts. Yes, the electricity bill will go up but bye bye gas, as this thing heats out whole house much better. Worth the money, if you have high RH in your tent don't put a dehuimifier in your tent get a good 20l at least or 12l if you keep your grow room door closed. This one is 259£ 330euro. It A energy rated. Won tons of awards and with not having to use the central heating anymore it'll actually even itself out. Great buy, it's really going to transform what I can get out of the bud as for quality and with my tent getting to 70%+ with the grow room door closed and then when in flower I can get it down to 45% and maybe that'll get even better. SAME DAY: So with my RH under perfect cond I think waiting any longer would be madness as I will risk losing a massive bloom in the mid-lower section that's full of stigma that If I don't act fast will end up turning into lots of amber pistils like little balls of stigma. I was preparing for this: I've been giving her water the last 3 days, today is the third. Overfed her to get run-off and was 1650, (meaning the top colas have stopped feeding). Hard flushed got ppm down to 210 and then after 2 hours through 500ml of flawless finish 2ml/l dose. This is meant to be given for a week, but here's the thing, I'm only harvesting 30% of the plant. Maybe will push it to 40% I'm taking the top colas any any well formed enough bud with no more than 30% of stigma. So 70% amber pistil buds will be taken. The 30% is just white pistils that could do with another week. But I'd rather take 10% and help the plant max out and hopefully she'll finish up. Cause if she stalls I'm gonna be left with a lot of poxy larf. So, sadly the flush will be halted t9morrow come 12pm I'm putting her into 12hrs of dark and taking what I need to. Hard flushing the flawless finish, let her drink up plain for till the next day and then back on a 800-1200 ppm depending how the plant reacts. I really don't know what's gonna happen. I know the top colas will be harsh but I've had a tester and to be honest I'm more than happy with it, I won't be curing the colas it will only being out the chemicals in her. 30-40% of petty dark good bud, little harsh but more than good enough, she'll been in nute lock the last week anyways so. She barley taking 3-400ppm out of her feeds and that's just a guess. Let's see what happens. And there wasn't any way I could of kept the top colas going as the plant is confused. I know that might not make sense. But the plant feeds from the top down, if the top isn't drawing up feed it's not drawing it full stop. Hence why the lower-mid growth has stalled. This is more of a see what can I save now. I could either just let her finish for the next 2 weeks. Takes what's on top and be happy with 50-60% larf. So, 40% of good bud and hopefully beem able to briny the other 60% to full harvest makes more sense to me. If it fails it fails the bud will finish off the way it would of, or worse it'll just finish. Let's see what happens over the next 48hrs. BTW dehuimifier works 1hr on 1 off goes between 45RH to 53 over the hours it's not on. Thanks for reading gottagrowsometime

Eva my little fast buds lady is such a wonder. In week 3 she is bigger than her Both sisters at the Same time. That‘s why I cut leaves only at first in her 3 week, because they are to many and to big and I am surprised about mother nature, because Eva‘s First Leaves are so nice and huge that i call her Aircraft Carrier…see with your own eyes. Respect and i will love her week 4 … I know.

25.05. 🌿 Day 25 – Stretching Begins! The plants have noticeably stretched since the last update — a clear sign that they’re moving deeper into the vegetative phase. The vertical growth is picking up pace, but they’re still looking strong and well-balanced. Despite the rapid upward growth, structure remains solid, with thick stems and healthy leaf development. The 19L pots and BioGrow feeding seem to be doing their job — no signs of deficiencies or stress. Light distance and environment are being carefully managed to control the stretch and keep the plants from getting too lanky. So far, they’re adapting really well. 💪 Overall: they’re thriving! —————————————————————— 27.05.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. 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