Moin ihr growmies, und ein knackiges Highfive in die Runde! Diese Woche war die letzte vegi Woche alles läuft soweit bestens habe die Pflanzen jetzt auf die blüte Phase vorbereitet Entfernung von Fächer blättern und Wachstum was nicht relevant ist da es nach unten wächst. Sonnst ist nicht viel los die Damen bekommen alle 3tage 3L Wasser sind schon ziemlich durstig, hier und da mal ein wenig Lst um den Baldachin gleichmäßig zu halten ab nächster Woche ist dann 12/12 licht Plan angesagt......!

day 58: trichomes are 100% clear. still a few weeks before harvest

Harvest time

Greetings, fellow growers! Week 5 has brought us to a pivotal moment in our Anesia pheno hunt journey as we embark on the transformative process of topping our thriving beauties. Let's delve into the world of topping and explore the magic behind this essential technique. Topping is a fundamental aspect of plant training, involving the removal of the apical meristem, or main growth tip, to encourage lateral growth and bushier plant development. By snipping away the topmost growth, we redirect the plant's energy towards the lower branches, stimulating new growth and increasing overall yield potential. It's like giving our plants a strategic haircut, with the promise of fuller, more abundant foliage in return. But topping isn't just about increasing yield – it's also about promoting a more balanced and uniform canopy, ensuring that light penetration is maximized and no precious photons go to waste. With each well-placed snip, we're sculpting our plants into lush green masterpieces, ready to flourish under the glow of our Future of Grow LED lights. Speaking of lights, maintaining optimal environmental conditions is key to our plants' success. That's why we've raised our TDS to 420 and adjusted our pH to a precise 5.8 using our trusty Blue Lab Guardian monitor. This advanced monitoring system allows us to keep a close eye on nutrient levels and pH fluctuations, ensuring that our plants receive the perfect balance of nutrients for optimal growth and health. It's like having a guardian angel watching over our garden, guiding us towards horticultural excellence. And let's not forget the satisfying sight of water droplets glistening on freshly topped plants. These tiny droplets are more than just a visual treat – they serve as a gentle reminder of our care and attention to detail, ensuring that our plants receive the hydration they need to recover and thrive. It's a moment of zen in the midst of our bustling garden, a testament to the beauty of nature and the joys of cultivation. With each passing week, our Anesia pheno hunt grows more exciting, and I can't wait to see what the future holds. Stay tuned for more updates, more growth, and more green goodness as we continue to cultivate joy and growth together in our Green Oasis! Genetics Red Banana Pudding = RBP Apricot Oreoz = AO Zoomiez = Z Future#1 = F https://anesiaseeds.com/ Plant nutrition - Aptus Holland - https://aptus-holland.com LED Photons - Future of Grow - https://www.thefuturofgrow.com/ Controls - Trol Master - https://www.trolmaster.eu/ Watering - Autopot - https://autopot.co.uk/ Tent - Mars Hydro - https://marshydro.eu/ Love and attention - Me, myself and i As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and blessed with you all in my life, With true love comes happiness. Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so More info, the ocasional give-away and exclusive updates from all my adventures can be found - links in the profile description Friendly reminder all you see here is pure research and for educational purposes only Growers Love To you All 💚

9/17 Didn't know this was a new week or I would've taken more pictures. I'm glad I took those two plants. As it dries and I start to trim I could see where it could've turned to rot real easy. It rained enough last night to soak all the girls. The two bottoms I harvest look like the have pm on them. If so I'll wash it or through it in the concentrate bag. Found a few colas a dropped which will go in the concentrate bag. Scoping the toasted toffy and the event horizon and the both look AMAZING! I've scoped the others and I'm extremely impressed. They may be difficult to grow strains but they have high cannabinoid content. The sherb cream pie has really come to life and turned totally purple with beautiful flowers. The girl in the 50 has several soda can sized buds. I hope this weather holds up. I don't like flowers this size getting wet. The sativa leaning hydrid us the only one I may have trouble with. It's only in a 10 gal but it's enormous! If needed I coyld bring it inside at night. The buds look like cat tails. I'm used to growing indica dominant strains. Plant us super healthy though. I'll be intrigued jail for a while and I've gotta make contact with whoever is going to do my extracts. I'll keep this updated. It's a lot of work bur it's worth it. 9/18 WATERED EVERYTHING I GALLON BUT THE BOTTOMS OF THE 2ND EV AND THE TT WHICH EACH GOT A HALF. Since harvest I've noticed some pm spring up on the toasted toffy. Looking at the other event horizon through a scope the trichs are all milky with some amber. I'm just waiting for A LITTLE more amber. And for me to have time and or room. I decided to harvest the tops and majority of one of my event horizon and toasted toffy. Today during an inspection I found a couple grey mold branches and some botrytis on my big GMO on the back. This plant is done. It's by far the furyhest ahead. Septoria effected the yield a little but pistols were retracted and amber was at like 10%. I thought about it and I watched the plant next to it with the pm have a branch I supported just "slough off". Right in my face. I figured if there was a sign tjis was it. I cut that rot off and put the buds from the branch in a bag and froze them then I went around and harvested the limbs on the other plant that were furyhest along. I took about half of it. I could've taken more and maybe I should've but I'm tired. I also noticed a couple spots of grey mold on the plant in the 50. All the leaves were falling off. I finally scoped it and it was right whetr I wanted it to be. Looking at the colors I'm glad I spent so much time the past few days. That one I hung whole. The sherb cream pie has something eating the fresy shoots (probably ear wigs. But the buds are fattening and the trichs aren't quite there. I'm going to let the rest of this stuff go ad long as possible. With the exception of the other event horizon. I finally tried it. It's fucking killer. I could take that plant now. The only reason I'm not is because I have to much work on my husband's as is. I'll do some videos amd pictures but I'm tired as fuck. Got to try event horizon. Very fucking impressed! 9/19 The past 3 days I've been working 5 a.m. to dark. I went back and harvested the bottoms of the toasted toffy and event horizon 1. After injury from harvest they contracted wpm. I used this as a learning experience and put some away in the freezer for concentrates and used a peroxide and water bucket. That's what kills the p.m. anyway. I hung them and they spun in the wind. I blew them lightly with a leaf blower before putting them ok strings I had previously setup with a fan in front of it. I kicked ass today. Toasted toffy got sampled and did NOT disappoint. I had it in a jar though and it was green so I'm not counting this. I do have some dry. I have indoor left too. Like in the video that one GMO looks like it's fucking dying or something. I'd rather cut it and use it for concentrates if it effects my healthy plants. I just checked on that plant in the 50 I hung. It looks amazing under the scope and smells better. Decisions, decisions. I do have a few calls I should make sooner rather than later. We are supposed to be getting rain. 9/20 The past 3 days have been hard. The wife's medical stuff is acting up and I had to tend to harvesting a few. My whole body hurts. I even did my first bud wash yesterday! The ev and tt I took and left some buds on ended up covered in pm. So I decided fuck it and tried bud washing. I also froze some for extracts. Speaking of which I need to male contact with whoever I'm going with this year because I think I've reached the point of diminishing returns on that dieing GMO. When it comes to grey mold and botrytis I don't fuck around. Winds are really high today. Putting the scope to the sherb pie is making me second guess myself too. Oh well. It's not going to rain today. And if needed I can move them in the barn. If I get ahold of Mt processor I'll chop that GMO and bring it right there. The other thing is that I've got tons of weed and a lot of its hanging so I've gotta find someone that will do a batch of live rosin then like a elbow of dry. This is the best part of the year. We'll It's supposed to be. Cutting down good plants us bittersweet. I don't mind chopping that one gmo a little early though because it's close to hitting the point of no return. That little sativa dominant hybrid in the ten is HUGE! The buds are swelling like crazy too. It's got a COUPLE septoria spots but this thing is resilient and easy to move around. This will finish perfectly. So far I'm happy with what I have. I'm GRATEFUL. The two September plants I tried were GREAT. A 90/10 and an 80/20 and they both suprised me and got me exactly whete I wanted to be. I'll keep things updated. I did a video I'll upload. 9/21 Weather is still great and the buds don't even seem wet in the morning. The thing is is that something (rot or ear wigs probably both) have stripped lower interior branches. In some cases stripping them ENTIRELY or chewing the stem right off. It wasnt happening to my favorite GMO or the one in the 30 and the like on in the ten. The Sherb pie I noticed grey mold on 3 different branches. Last time I checked trichs it wasn't ready but I've got to get a handle on this mold/pests or it's going to he taken a little early. We have incliment weather coming Wednesday I think. I'll bring the 10 inside and if I have to I'll bring that perfect GMO in as well. I'm going to scope everything again today. I called a bunch of extractors for prices. I was going to do fresh rosin and just yank and drive but that is looking to be harder. I can get badder made CHEAP from a reputable lab. I think I'll do that. They prefer dry and that way I could through what's left from last year in there. I have this other one GMO that I've had the same issues with. I'm sick of removing bud rot or having to cut interior branches off due to pm. Tried toasted toffy and it was fucking amazing. I'm planning on trimmingvthe ev and if I finish I can bring the other one in. It's done. It's not getting any bigger. Trichs aren't changing. That GMO I am going to scope when I get home and it will be coming down in the next couple days. Otherwise I'll end up with half of what's there. The GMO I took the tops from is finishing up too. If I scope that and it looks decent and I have the time I'll take the remainder. I'm leaning towards doing at least a lb dry for extracts. I've got some frozen. I've got some hanging and most is still in the ground. I sleep on all major decisions. However, I know getting my nice buds soaked when I'm already losing shit to budrot and grey mold isn't the smartest thing. I'll probably take what I can and bring the others inside to keep them safe. I didn't think I'd have this much quantity. I knew quality would be good but this isn't a bad year quantity wise either. I'll keep this updated. 9/22 Busy as hell! Rain coming Tuesday. After a final check of the scope and the flowers and I took the GMO that all the leaves had fallen off of. That plant is going straight to extracts with the one in the thirty. I also "finished" harvesting that GMO that was furthest along. Trichs were getting amber. Been in trim jail. Was going to take the Sherb Pie but I'm not seeing amber yet. I did have to cut off some branches that had rotted. I'll keep an eye on it. It will come down when it's ready. It just sucks losing little bits of it. My favorite GMO is sooooo close to perfect. I should just take it but I'll wait. If we get rain I'll bring that and the sativa looking one in the ten is the barn. Back to work now. Before I forget what strain is what lol. 9/23 Still busy as fuck. I chopped the other event horizon amd hung it whole. It's DONE and it doesn't need to go through the rain storm. I also took the majority of the GMO early pheno. What's left will get plucked off and used for extracts. The big one in the 50 is getting dry. I have finished the event horizon and I'm not "finished" with the toasted toffy but I have it in 5 gal buckets with a food grade lid so u can at least cure correctly. The gmo is still hanging. I'm wondering whether I should wait it out or chop. I'm gonna take care of what I have inside first and hope that shit doesn't eat my plants or that it doesn't rot off. That perfect GMO has one branch with some grey mold. I might cut it off. I'll keep this

La Kosher Kush de la derecha abajo, tiene las hojas más finas y es más alta, las otras 3 Kosher Kush tienen las hojas más gruesas, la del medio abajo tiene un poco de color morado en el apical. Las Kandy Kush se parecen bastante, un poco más altas la de los laterales, pero se las ve bastante bien. Las Lemon Krystal son pequeñas y no se nota mucho, pero la del centro izquierda, tiene las hojas un poco más anchas. Si os fijáis en la de abajo izquierda, tiene una planta pequeñita al lado que es la anterior 1024 que se quedo una ahí en la tierra y la otra la saqué y la puse en una maceta de cactus, que también ha germinado, pero les ha costado, por eso están fuera y tenemos las Lemon Krystal, en el próximo trasplante, la sacare y la pondré en alguna maceta por el patio. Se han trasplantado a maceta de 6L.

18/6 from seed Only water twice a week Only once with nutes When in low pot now in bigger pot we will see how much she needs Very abundant roots you will see the video and notice how well she is growing I really doubt the stress from this fast transplant so we will see it's behavior We already added bloom nutes since she didn't have anything before but whoa so fast did not expected that from red poison but makes me feel great to have you I think you will be my favorite haha Happy growing guys Sweet smokes Thanks for that guys

I just harvested her today. Frosty looking nuggets with a pine citrus earthy aroma. Super sticky as well. I had about a 5 to 10 percent amber trichromes. I might of been a pinch early, but she was starting to foxtail. The colas look really nuggy. I had issues with mites early when I switched to flowering. This likely impacted some of the yield potential as she was neem oil treated for a week during that time. She did way better then I expected after that big stressor in play. Mites are long gone now. The plant will now hang dry 24 hours then get a light to mild agression wet trim, and end up in the cannatrol. To start a 8 day dry, and cure process. I will then run it in the bud trimmer. Then hand trim to finish. The G3000 light is a great quality light. I just ran it 19 weeks on a photoperiod grow, and had no issues with any of the Spider Farmer Equipment used. I used the 2.3x2.3x5tent, G3000 light, germination mat, 4 inch inline smart controller exhaust fan, and I will be using the new 16 inch Spider Farmer bud trimmer bowl when time comes. Excellent equipment from start to finish in my grow. I want to thank Spider Farmer, and Pure Instinto Seeds. 🤜🏻🤛🏻💪🏼❄️🌱 I also want to sat the biggest thank you to the grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Spider Farmer G300w: https://amzn.to/3S2zvsd Spider Farmer 10X20 Heat Mat Kit - https://amz.fun/lsa0J Spider Farmer Amazon Store: https://www.amazon.com/spiderfarmer Spider Farmer Official Site: https://spider-farmer.com Discount code: saveurcash (stackable)

1 - Gelato 41 - Somente água (only water) 2 - Big Buddy - Somente água (only water) 3 - Gelato 41 - Somente água (only water) 4 - Ak-47 - Somente água (only water) 5 - Generic Auto - Somente água (only water)

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 99. Garden is flying. Nothing else to mention now, you can easy spot - they bulk up ! Heavy feed in the morning, then 2 more cycles of feed/pure_water left till flush for Gorillas, all others at least a week behind. Hate to split waterings and feed, but even lazy person sometimes is forced to ... ;)) Happy growing ! Day 100. Disaster in Garden !!!! Had enough of RQS Royal Cookies. Two of them went Hermie. So current stats : 5 out 5 are mutated 2 out 5 currently Hermie 5 out of 5 overfed without any food even Because of that shitty genetics, had to make Control Garden. Even Cookie from CG which is in green house still grow mutated and leaves are on max healthy green side. Had no nutes for last 30 days, still haven't returned to normal ... So two girls chopped, hope they didn't pollinate all garden... In photos you can find bananas... Those air pots works really great, all space is used and roots are really nice. Just i think i will go to 30 liters next time. Have fabrics, but my doubts of those air pots are gone. Bad day for sure. 15 weeks, 50 pounds for seeds, 30 for soil, 50 for nutes. Thats my bill without electrics ..... #RQS is crap !!!!! James from them just asked where i bought the seeds, never had balls to replay back ... Ironic theirs girls has BALLS and it makes me angry ... Day 101. Damage is done, i see many seeds growing, it took me unprepared .... I dont sell weed, but to check every bud before grind is lame for me ... F@%k you RQS. Never again, despite having like 10 more theirs beans of 4 dif strains .... to the bin now !!!! Day 102. Girls are nice, but i see seeds forming and it makes me sad. Doubt it will be many updates till review. Happy Growing !!!!

I use Jacks aqua gold at 2.5 grams per gallon Epson salt 5g per gallon and jacks 15-0-0 2.5g per gallon. I ran out of advanced nutrients 3 part and will not be purchasing more. I will harvest everything on the 12th. Might pick off some of them before depending. I am not flushing but reducing ec. Currently feeding 1.2 ec

Buenas farmers!! Después de pasarnos tres dias con sólo agua volveremos con la mitad de nutrientes durante los próximos dos riegos, ya empezamos a prepar nuestras plantas para la fase final ! Un olor espectacular y mucha resina espero guste buenos humos family!💚

Buds are fattening, hairs are turning orange and the smell is very pungent. Getting some black tips on leaves, any advice would be great. I gradually lowered the ec last week and this week, wondering if that’s what caused it, ph has been dialed. About 2 weeks until harvest.

Technical and general parameters of the breeder: Mandarine XL Auto is quite a tall strain which doesn't come as a surprise due to the fact that Sativa genes dominate in it. This cross of Cream Mandarine Auto and Thai can reach 18-23% THC. As the name implies, sour-sweet citrus notes are dominant in this tangerine strain, however you can also taste a nutty flavor. The production of buds and flowers is stunning! Lavish resin covering the tops of the plants is incredibly aromatic and thick. Mandarine XL Auto gives a relaxing balanced effect. It will make you happy but it can also boost your appetite Genetics: Cream Mandarine Auto x Thai Flowering type: Automatic Strain type: Autoflowering Sex: Feminized Indica/Sativa/Ruderalis: 35/63.4/1.6% From seeds to harvest: 9 weeks Indoor height: 110-150 cm Harvest Indoor: 450-650 g/m2 Harvest Outdoor: 50-300 g/plant THC: 18-30% CBD: 0.6% Taste: Citrus fruits, Sweet, Sour, Mandarin Effect: Creativity boosting, Happy, Hungry, Psychedelic, Relaxing For cultivation I used: Cultivation area: 80x80x180cm 1x circulation fan: 15W LED lighting: Mars Hydro TS 1000 - 150W - vegetation and flowering 💡💡 Exhaust: pipe fan - RAMTT100 Odor filter: PRO-ECO HF carbon filter 160-240m3/h - 100mm - maximum air flow up to 240m3/h 4x textile flower pots 11l Substrate: Plagron Lightmix perlite coco coir Thank you to all my friends who support me in growing, who give me advice - @Happy_Rakosnicek, thank you to my friends from Grow Diaries for likes, advice and support 😍❤️❤️ Many thanks to the Ganja Farmer team for providing the sponsor seeds 🌱🌱😍 Thanks for the likes and you can follow me on Twitter 🐦: @ Targona666 Many thanks to Mars Hydro, specifically @Coco_Pan2022 for providing the Mars Hydro TS1000 sponsor light💡💡😉 Smoking Review: I will add after about 10-15 days of drying the buds 💨💨

Starting Week 7 Veg defoliated 10% Brewing a light prebloom cocktail (ACT) for 30 hrs . Nutrients should be ready for switch 2 bloom next week.😁 (EJ Grow, Bloom, Meta-K, MIcroblast,Catalyst, Hi-Brix) @ 30% strength

ultimas semanas de las nenas,en un par de dias se le realizara lavadl de raiz.

hi guys, This strain is fast becoming a fat lady😃💪 I love the smell, it reminds me of lemon and orange now with that touch of "fizz" if you know what i mean👍 .... it forgives mistakes (I gave her a little too much .. so she got burned a bit ...😐 she does not like much fertilizer .... very surprising .... noticed for the next time👆 ... but otherwise everything looks pretty good .. let's see how long she still grows, since she already looks premature .... because of this year's heat wave, all my plants have had problems in the root zone. these problems are now unfolding their effect .. the root zone has taken so much damage that the roots can no longer properly feed the plant, resulting in finishing wayyyy too fast ... shit happens The resin production is of the hook and she is producing more and more😍 The grow: ph 5.8 Ec.0.9 i will lower it to 0.8 and inrease N a bit at the same time RHD:45% very good for this stage.. have a nice one people till next week