Hello growmies 👩‍🌾👨‍🌾🌲🌲, 👋 Keep stretching, and big stretch on some stems, hedgehogs coming along with stems 🦔 💪 Continuing scrog and fight with leaves. 💧 Give water each 2/3 day 2 l Water + Roots + Bloom + Zym + Sugar Royal (1 + 3 + 1 + 1 ml/l) PH @6 💡Mars Hydro - FC 3000 50% 23 cm Mars Hydro Fan kit Setting 7 Have a good week and see you next week 👋 Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Mars Hydro - Smart FC3000 300W Samsung LM301B LED Grow Light💡💡 https://www.mars-hydro.com/fc-3000-samsung-lm301b-led-grow-light Mars Hydro - 6 Inch Inline Fan And Carbon Filter Combo With Thermostat Controller 💨💨 https://www.mars-hydro.com/6-inch-inline-duct-fan-and-carbon-filter-combo-with-thermostat-controller Fast Buds - Gorilla Cookies FF🌲🌲 https://2fast4buds.com/us/seeds/gorilla-cookies-fast-flowering

2 Black cherry gelato clones from my clone guy. 1000w LED, 4G Autopots,, coco/perlite 65/35, GH 3-part+ flourolicious, calimagic, SI, chelated iron, and humic acid , RO water, AC pro Controller and T8, 5x5 GG Tent with extension Garage grow SoCal This week the girls had a hot week in the garage averaging 88°. This week the girls started to show up to the party; bulking up and putting on weight. It’s like they stopped at Wendy’s and got a frosty overnight. We’re running just under 1000ppm with no issues. I’m having reservoir funk issues so I’m switching to jacks RO this week. 1g a day. This weeks accomplishments: Split electric load (safety) This week: Switch nutes upgrade reservoir and lines next week. Long term issues It’s hot. Straight 90s this week. So the same - Cooling and VPD targets during heat waves Preventing pest and mold issues during high humidity conditions. I hope you all are having as much fun as I am. Best of luck gromies. Check out my Purple push pops grow. Just posted my outdoor light dep grow - side by side with my indoor grow- same clone batch. Purple push, pops and black cherry gelato inside and outside . Garage versus backyard . This should be a great comparison of indoor versus outdoor grows side by side

day 70 was harvest day !! really fast strain, 24h light ON really played a big role as well frosty buds and amazing strawberry smell, now lets dry it !!

This Baby just can't stop grow. i wanna smoke this strain so bad so badddd. To give you an example of why i'm telling you this, check this out. i have my grow tent with the door open in my house with my infinity at maximum speed 10/10. AC ON 24/7. This days are really really hot over here and if i close the grow tent the Temp will raise up to 85%. Anyway.... 2 days ago i had a visit in home and i was a little afraid for the smell, turns that when they got here the first thing they ask for.... was the fruit that smell like that?. 😂😂 I said that i made a casquito de guayaba (that's a famous cuban dessert made from real guavas) smells very very sweet and taste asome. This plant smells like that. Like a candy or a very sweet fruit But she's taking her time and im glad of that. I want to tanks to @jinks101 @Trueorganics84 for the help they give me in this, my first grow. 🙏 thank you all guys...

Fue una exelente cosecha, esta planta en un principio se tuvo en imddor y posteriormente sacada al exterior. Es mi primera experiencia plantando en suelo. La planta llego a un gran tamaño de la cual se cosechó una gran cantidad superando la cantidad promedio.

unfortunately we noticed some budrod in the headbud. we cut it generously sb, unfortunately the papaya cookies still need something and the weather is now autumnal cold and rainy. We'll see in 7 days at the latest, but hopefully there won't be any more budrod by then... It's the most beautiful of all the outdoor plants so far.

Let's go ☺️

* 3rd December - Day 14 from seed * Well, what a week! And I wasn't even here to enjoy it. My partner lovingly tended Nora (we decided to name her) while I was away. Looking at 'germination week', you'll see I was constantly aerating the nutrient solution. For the first 4 days, my partner was pH adjusting the solution (Rhizotonic seems to leave pH constantly climbing - around half a point per day), pouring out any old solution from Nora's 'semi-kratky' pot, then syringing 24ml of solution through the rockwool. As soon as the tap root was around half way down the pot (4th day), we filled the pot so the tap root was just touching a well of nutrient solution within the pot. Each day, the solution was emptied and refreshed - not taking any Pythium chances here. Within the next 3 days the rest of the explosive root growth came bursting through the rockwool. So, time to transport her. Made some changes to the bucket: lots of holes in the rim for LST, full foil-bubble insulation (including around the rim of the net pot , and aluminium tape around the entire silicone air line - zero light leaks), a very large new air stone, and a 25w pump to power it. The pump is ridiculously quiet - not 'quiet for 25w', simply ridiculously quiet. And as you can see, it creates a bucket which looks like a pot on full boil. There's absolutely no way pythium is getting any sort of foothold on this grow. The root explosion made it very easy to position the rockwool cube within the hydroton - the roots are submerged while the rockwool sits just above. Decided to stick with the Formulex as it's doing a grand job, albeit the dose has been kicked up. Swapping the Rhizotonic for Regenaroot as I hear very good things, including that pH remains stable! Will now use Rhizo for foliar applications only.

January 24, 2024 Day 141 Gorilla Cookies: Number 2: This is the final week of flower for this lady. She may need another week to completely finish up, but this is the home stretch. The fade has started. Pistils are about 50% orange now. She's not taking in as much water, as I noted before. Today, the moisture content is 20%. I'll have to see how she does throughout the day. I'm really trying to not let her get that dry. Maybe I'll wait for tomorrow and let her dry out this one time. Maybe it will help speed along the maturing process. There really isn't much else to report today. I plucked a few more leaves off today. All of which were either dead or dying. Not too many though. There was maybe 10 total. Grow System Environment: Temp: 73.3° RH: 45.2% VPD: 1.50 kPa January 25, 2024 Gorilla Cookies: Number 2: Not much to do today. Soil moisture is at 20% as of this morning. I'll check later this afternoon if she needs water. I really don't want to water in the afternoon. If the moisture is below 15% by 6 pm, I'll have to water, but hopefully she holds off at 17% for the evening. I pulled a couple more leaves off today. Not much, just some lower dying leaves. The fade is in full swing now. No colors yet. Just a lot of yellowing leaves. In the next few days, I expect some colors to start showing. The top net isn't doing really anything. Some colas are standing a bit from it, but the majority are still leaning too far over. It's because I can't reach in the back and couldn't tie it up. So the net is just floating. Kinda wish it was a 4" grid. Oh well. It's there now, so it's not coming off. Grow System Environment: Temp: 73.9° RH: 45.0% VPD: 1.53 kPa January 26, 2024 Gorilla Cookies: Number 2: So last night I fed a half gallon to hold her over for the night. This morning she was at 16% moisture content. So she got a gallon of Flawless Finish. The fade is going full swing now. No colors yet, but more leaves are turning yellow. Colas are continuing to put on more weight. Not much, but still more. Everything else looks good. There's a couple more damaged leaves, but I honestly don't feel like getting rid of them yet. I'll wait for them to die out more. Although, there are very few of them left. I did pretty good at clearing them out originally. Also, after looking through the plant, there really isn't much more I can do for now. There's no sense in pulling healthy leaves right now and I can't do anything with the canopy. So basically it's all good to go now. Just watering and observing. In a week, I'll check trichomes and start trimming healthy leaves. It should be a day or 2 before harvest. Grow System Environment: Temp: 73.7° RH: 45.7% VPD: 1.50 kPa January 27, 2024 Gorilla Cookies: Number 2: The Beast is almost done. Lots of fading. Trichomes are almost 60% cloudy to 40% clean and some random amber ones. Her morning moisture content is 18%. She'll be getting a full gallon around noon today. I really want to let her dry out, but she has grown so well with the schedule she currently has. And she drinks way too fast to let her dry out today. Maybe I'll do a half gallon instead and a full gallon tomorrow. I pulled more lower dying leaves off today. Maybe around 20. It has to be a good sign along with the fade. She'll definitely need a bit more time than hoped for. Maybe another 7 days instead of 3 to 4. I'll start checking the trichomes in earnest in a few days. Colas are definitely heavier and a bit more dense now. I think that's all she has left. My major concern with yield is the lower half of the plant. Nearly all of it is popcorn or larf. Even the fully formed lower colas are loose, airy and look like whole bud foxtails. I'm honestly more worried about quality more than yield, but the yield is definitely less than I could have done. Much less. And the bud formation is just low quality. Most of it will get sifted for kief. Grow System Environment: Temp: 73.6° RH: 46.2% VPD: 1.48 kPa January 28, 2024 Gorilla Cookies: Number 2: Absolutely nothing to do today. No leaves to snip, no water to feed. Nothing. Soil moisture level is 20%. I'll keep an eye on her for the day. Just in case she drinks down to 15%. The right side of the plant is nearly horizontal. Especially the front right corner. However, I did lift on up and used the top net to do it. So that's a good thing. There's way more yellowing leaves today, but no colors at all. Maybe I started flushing a bit too early, now that I think about it. Maybe color will start coming out in the next few days. I still think she needs a bit more time. Update: I got bored and decided to inspect the trichomes anyway. There's a lot of amber on the leaves. Not a whole lot on the buds though. After viewing them, I think she has about 3 to 4 days left. I'm just waiting for the lower part of the plant to mature up now I guess. I also found more dead leaves. I had to dig a bit in the back left corner to get them. Anyway, I'm trying to get in the way back to help prevent mold or whatever else can happen. So far everything looks good. But I don't have much of a view. The colas are too tall to really inspect fully, so hopefully keeping the RH below 50% and the air movement going will prevent any issues. The biggest issue is moving the plant forward to make room in the back. The colas just fall against the wall of the tent. So I will have to try to get back there to adjust the colas and make some room for them. I still can't see in the way back, so I have no idea what I'm working with. I guess it doesn't really matter much so close to harvest. Grow System Environment: Temp: 73.4° RH: 46.8% VPD: 1.45 kPa January 29, 2024 Gorilla Cookies: Number 2: Just a few days left for this lady. I checked the trichomes again this morning. They look about the same. Maybe a couple more amber here and there, but as I said, it looks like she'll be ready in a few days. Maybe 3 or 4? She got a full gallon top feed of Flawless Finish. I think this is the last watering she will get. Maybe 1 more, but I don't think so. Nearly 70% of the pistils in the lower part of the plant are orange. I didn't bother checking those trichomes. I'm debating if I should just cut the whole lower part of the plant and let it all dry on a tray at the bottom of the tent. Maybe not. I'm not too sure yet. What I do know is that I can't reach 90% of the foliage with all the larf in the way. So I'll remove what I can on harvest day I think. She'll also need a 48 hour dark period. That will start in 2 days or maybe tomorrow. It all depends on the trichomes. There's a ton of yellowing leaves now. No colors though. I wonder why that is. If that's the case, then maybe she needs a couple more days for the fade, then the harvest. So now looking back on all I've decided today, I think a total of 5 days left. 2 to 3 more maturing, 2 days of dark and then harvest day. If that's the case, then I'll be watering one more time I believe. Maybe I'll go down to a half gallon tomorrow morning and see how she does tomorrow night. I may add the second half of the gallon tomorrow night. Speaking of watering. I noticed the moisture content stayed at 14% overnight. Not sure if that's the probe itself going as low as the soil can go. It was really light this morning. And I definitely didn't get a single bit of runoff. That's all for now. Grow System Environment: Temp: 73.4° RH: 42.8% VPD: 1.57 January 30, 2024 Gorilla Cookies: Number 2: It is the end of the road for the Beast. Tomorrow will start with the 48 hour dark period. Then I'll try to defoliate what I can reach. I may end up having to just leave it alone til after she dries out. Mostly because I can't reach about 80% of the plant. The lower half is loaded with larf and popcorn. So navigating it and actually getting through the trellis is damn near impossible without damaging the lower part of the plant. Reaching over is a bad idea as well. My whole arm presses against the colas just to reach a middle cola. So nope. That's not happening. Looks like a true dry trim. That will take all day, if not longer. I learned that my tap water has a PH of 8.1. Now that I have PH up and down, I brought the PH down to 6.2. It's too late for the PH to make any difference whatsoever, but at least I'm getting started. The next grow will have a perfect PH and supplemental lighting. I can't wait to see how my new stuff works from start to finish. I've also decided to try to lower the temp down to 70°. Just for the last 2 days. With the lights out, it should be around 66°. I'm also trying to drop the humidity down to around 40% maybe 35% if I can swing it. Grow System Environment: Temp: 71.2° RH: 42.4% VPD: 1.46 kPa January 31, 2024 Apparently I can't make another flowering week, but it's been 7 days for this week. Oh well. I'll be harvesting in 2 days. She's definitely ready. Just want some more trichome maturation, then I'll be happy. And what better way to do it by a 48 hour dark period. No watering today. Although, I'm a bit concerned with how low the moisture content currently is. It's at 15% moisture content. It could get dangerously low before I give the good ole chop. I'll probably check her later today to see if she is suffering. If not, I'll check tomorrow. However, I don't think she will last for 2 days. Maybe I'll give a half gallon and let that be it for the rest of the dark period. I'm keeping the temp around 70° and RH around 40%. 35% if I can manage it. Grow System Environment: Temp: 70.3° RH: 42.1% (and dropping) VPD: 1.42 kPa February 1, 2024 Gorilla Cookies: Number 2: No pics today. Just a quick update. Moisture level is down to 11% and lights are still out. Another day and harvest time. I'll check in a bit to see if she is suffering from lack of water. Not sure what I'll do if that's the case. I guess harvest right away. I can't add more water and it's only been two days. Anyway, I'm pretty sure she'll be fine until tomorrow. Update: forget everything I wrote today. I chopped her down. She was drooping everywhere. So I harvested. Wet weight was an astonishing 110.4 oz. Based on my average 13.5% weight retention, I should end up with around 15 oz. Holy shit! Grow System Environment: Temp: 68.5° RH: 39.8% VPD: 1.39

Everything was going well in the first week, but unfortunately, a mistake caused stress in the plant. The distance between the lamp and the plant was too short. This week, I gave the plants a small amount of root growth stimulant

Welcome to Harvest in Flower Week 9-10 of Divine Seeds Auto Black Op1um I'm excited to share my grow journey with you all as part of the Divine Seeds Autoflowering Competition 2025. It's going to be an incredible ride, full of learning, growing, and connecting with fellow growers from all around the world! For this competition, I’ve chosen the Feminized Automatic strain: Auto Black Op1um Here’s what I’m working with: • 🌱 Tent: 120x60x80 • 🧑‍🌾 Breeder Company: Divine Seeds • 💧 Humidity Range: 50 • ⏳ Flowering Time: 8W-10W • Strain Info: 25%THC • 🌡️ Temperature: 26 • 🍵 Pot Size: 0.5l • Nutrient Brand: Narcos • ⚡ Lights : 200W x 2 A huge thank you to Divine Seeds for allowing me to be a part of this amazing competition and Sponsoring the Strains. Big thanks for supporting the grower community worldwide! Your genetics and passion speak for themselves! I would truly appreciate every bit of feedback, help, questions, or discussions – and of course, your likes and interactions mean the world to me as I try to stand out in this exciting competition! Let’s grow together – and don’t forget to stop by again to see the latest updates! Happy growing! Stay lifted and stay curious! Peace & Buds!

The fourth week into flowering – and our results are simply stunning! Our girl has transformed into a true beauty: thick stems, lush greenery, and plenty of gorgeous buds. Thanks to LST training, our little one has become even bushier and more compact – doubling in size over the past week alone! And it's all thanks to Xpert Nutrients fertilizers. Currently, we're actively watering with bloom-stage additives: Master Bloom, Master Root, and Sugar Shot, diluted at a rate of 2 ml per 1 liter of water. Of course, the water has been purified and has a pH of 6.5 and TDS of 1500 ppm. Everything is going according to plan – we can't wait to see what else lies ahead!

Всем привет! Моему малашу сегодня 11 дней. Выглядит здоровым и счасиливым. Поливаю водой, ни чем не кормлю Признаков болезни и нехватки элементов не замечено! Всем и мира и добра!

Another good week without any worries so far, crossing my finger. Although watching the time lapse, I see them sag during certain times, and then sprout other times. I am trying to watch when I feed them and to only do it when the leaves start to droop. For instance, as I am writing this, I see on my live cam they seem to be doing well, I have been checking the PH most times when I water them, but sometimes I just trigger the pump remotely without testing as it was good the last time I checked it. I can see them starting to develop buds, and hopefully they start to thicken up this week. According to my chart, I will be dropping the Medi One to 1.5 ml/l this week, and continue with 5 ml/l for the massive bloom. I also had to raise up my light a bit, and hopefully they don't get much taller as I only have a couple of inches left on the cord.

Same old same vege stage

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

The buds are growing bigger still and the tent is really starting to smell, the stems have turned red from being close to the grow lights and my center buds have foxtailed, i now know for future runs to stay more on top of training so the plant cant grow as tall. pistils are starting to turn, If i had to guess I would say 11 weeks seems like an accurate flowering time I have also let them get dry a few times.

Hey everyone :-) This week it smells more and more sweet and fruity in the whole room 😍. There is actually not much to report, everyone is now under 12/12 :-). This is the last grow that comes in the entire diary. From now on, each plant will be added to the diary 👍. I wish you all a lot of fun watching, stay healthy 🙏🏻 and let it grow 😎👌

So, they streched a lot, I had to bend severely. And so quick, like 5 cms in a day! They touch the light, I had no more room... I did a late FIM on 2 of them.... emergency I finally found a way to get the lamp 5 cms higher. Lukily it has stabilized but still, top of the buds are at 5 cms from light!!!! That's why I decided to drop the power at 50% and I lower the day light to 16 hours.