07.02 Попытался делать LST. Главный стебель довольно толстый уже, особо не выгибал. Посмотрю далее на реакцию 12.02 — Проведение LST (с клипсами)

Wächst wunderbar ohne Training

It was a smooth start. 3/3 seeds succesfully Germinated

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Ran into some pH issues in the medium.. Runoff would come out at 5.5 at times.. But now that I think about it.. The plant was very Indica.. Fat leaves.. The leaves stayed super close to the main stalk.. Not letting ANY light to the lower branches.. But defoliation is obviously a must.. I grew this with another strain.. Mandarin Cookies and she was ready to flip weeks earlier for sure.. If I could have vegged maybe 2 more weeks she would have been able to fill her half of the tent alot better.. But all in all.. Great strain! Very terpy! Very complex aromas! Super dank strain! Pretty Indica, I'd say... Narcotic like effects. Beautiful plant.. Awesome tastes! Impossible to even try to explain!

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1 er Cycle : Gourmande e crossance et Flo ... La Tangerine Dream est une variété de cannabis à dominance sativa au goût acidulé, doux et intense. Les arômes d'agrumes ainsi que les effets qui sont à la fois cérébral et relaxant satisferont aussi bien les amateurs de THC comme les amateurs de CBD. Les juges de la Cannabis Cup 2010 étaient d'accord avec cette description car ils l'ont élu comme la meilleure variété de cannabis 2010. Barney's Farm annonce un temps de floraison de 60-65 jours, ce qui est court pour une variété sativa aussi puissante. La Tangerine Dream donne des plantes de un mètre de haut en intérieur avec beaucoup de branches latérales robustes. La production de la Tamngerine Dream est impressionnante, 600 gr/m2 en indoor. Les têtes sont grandes, compactes et recouvertes de pistils rouges orangés ainsi que d'une résine très odorante remplie de THC. L'effet de la Tangerine Dream de Barney's Farm dure longtemps. Quant à ses parents, la G13 est une variété de cannabis légendaire reconnue pour ses saveurs intenses ainsi que ses effets très puissants et la Neville's A5 Haze est une plante exotique raffinée. La Tangerine Dream de Barney's Farm est une variété de cannabis très productive et de floraison courte. Les taux de THC et de CBD annoncés par Barney's Farm sont exceptionnels car peu de variétés du marché actuel arrivent à 25% de THC et 1.8 de CBD. Parfait rien a dire. 2 ème Cycle:

arvest Report – Orange Apricot Glue XL Auto The grow is finished. The Orange Apricot Glue XL Auto had a tough spot right from the start. Growing in the shadow of the large Black Muffin photoperiod plant and getting only 12 hours of light instead of the usual 18–20 hours for an automatic, she couldn’t fully unfold her potential. Still, she pushed through and developed a nice layer of trichomes. The buds smell and taste great, offering a smooth mix of citrus notes and sweetness. Considering the conditions, I’m happy with the result.

02/23/2022 Lots of stretching this week,not much to say so I let the pics and videos talk for them selves🙏🏻 Enjoy Grape Skunk tall and lanky one I'm loving! I will number her for future reference. Biscotti Skunk has some droopy leaves, it's watering day.

My plant is still taking its sweet time ever since I cleared the roots of root root The roots look so healthy and truly have grown so much I am very proud. Getting another light this week the lower flowers aren’t getting enough light

🍀04/07 - Empieza su sexta semana en Etapa de floración. 🍀Me sorprende lo llena de tricomas que esta, su olor es fuerte y ya no veo la hora de poder probarla. 🍀Se encuentra perfecta de salud, solo que tuve que hacer un cambio de lugar a las lámparas porque crecieron demasiado alto para el indor que tengo, algunas hojas que se encuentran abajo están un poco amarillas pero nada grave. 🍀El ph del agua es de 6.2 🍀Voy a estar dejando videos de como se encuentra. 🍀En estos dias seguire subiendo mas imagenes. 🍀🇦🇷😶‍🌫️Podes seguirme en Insta gram como @bruweed_arg 🍀🇦🇷😶‍🌫️

Hello Folks 😎 7th week of flowering,I'm really amazed by these girls ! They are beautiful, the smell is powerful, and these buds are dense like rock !! 💪 They drink 4/5 liters every 5/6 days, for the last watering with fertilizer, I reduced the dose about 650 ppm my plan is to start the flush from day 50 until day 65 and from there, I'll see if I have to push a few more days while checking the color of the trichomes I think I'm going to enjoy this already legendary strain, like the smell a lot 😻 Follow, like and comment if you enjoy it I also have a second diary with 2 Moby Dick Peace Love & Weed 😍👊

Harvested 1 plant after 49 days flower & harvesting the last one at 56 days of flower.✂️ Flushing with ph adjusted tap water. 🌊 Really happy with this strain. 😊🌿

Pistols, Pistols, Pistols, seen the flowers began to develop, she stretched lot last night, feb7-8 ir video uploaded. Took all the excess leaves off my girl

Esta cosecha fue muy buena con excelentes resultados de flores muy compactas y demasiado resinosas , la genética en sí es muy resinosa con olor muy característico , con sabores terrosos muy marcados .