2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Day 7........

Week 8 of flower is in the books and this show is quickly coming to an end. I said last week we had about 7-10 days and I was spot on at the 10 day mark. I was only wrong about the order. The cheese is ready to chop before the rest and the gorilla cookies, cherry Bomb and lemon haze will follow in due course. The cheese is a beast and it’ll take a little time to get it all run through the bowl trimmer. At least the big one goes first. Her tallest colas have foxtailed like mar, despite the late supercrop. You can tell where the sweet spot for the light is at about 10” distance from the canopy. Any closer than that and the tails begin to show prominently. It’s a good example of just how close you can get to these fixtures with a little bit of prior experience. It’s been noted for the next run😎👍😎. The res. is on an h2o2 only flush for the remainder until the rest are ready to come down. Not a moment too soon either - the pollen chucking project has stalled out waiting for these girls to finish and now that we’ll have a little extra room, we can start running our cuts in the aerocloner🤟. Not long at all now folks. Thanks for tagging along through all this. Learned a bunch about this system and we’re back to hardware mode to make some serious improvements for the next run🤙😎. I love hardware mode😉. Background/Repetitive Nonsense The strain line up on this grow is look’n quite saucy 😈. We’ve got 2 FB strains this time around - Gorilla Cookies (seems we always have some GSC cross in the works). Mainly because the colours look stellar and remind me of a sugar breathe we did last year that was 🔥. The other FB is their original Cheese strain... because we already smoked all our cheese... and we really like our cheese...😉. Emerald triangle was kind enough to include a freebie of Lemon Haze. Wasn’t really big on the list but we haven’t done a haze strain to write home about yet so worth a go👍. The last is a Cherry auto from Bomb seeds that we’ve been trying to get to for months. Let’s just hope that she’s not a dud like the last bean. The new digs for this run are courtesy MarsHydro👊. Their 2x4 single grow tent, paired with a shiny new 240w QB courtesy ViparSpectra. These folks were kind enough to discuss a collaboration and as a result, here we are to test out the gear with an inaugural run of autos. Each week, we’ll try to expand on some of the benefits of each, as well as any limiting factors or considerations that need attention when pondering purchase. Already I can say that both are high quality products in terms of overall craftsmanship. MarsHydro 2x4 Grow Tent: Excellent construction in terms of durability. Reinforced stitching on the zippers and corners ensures zero tearing. Conveniently placed screen vents and intake/exhaust ports. The ceiling exhaust port only exists on the right side. Perfect for me but not great if you’re running exhaust from the top on the left side (left side wall port makes up for this however). The aluminum frame is rigid enough to support the weight of the lights and accessories. Once you have some weight in the tent, she’s go’n nowhere and you can assemble and break it down in record time if moving or stashing the grow op. Only limiting factors I’ve noticed is that temp. Control will be an issue for a tent. It just doesn’t have the same insulating ability as a wood cabinet or other permanent enclosed space. No worries, this is offset by a small desktop heater and keeps the environment exactly where desired. The other issue was a production problem - the ceiling vent was accidentally stitched into the corner seams on the assembly line. Again, no real issue - just pulled it out with no damage or fraying to the tent or port👍. Website link here: https://www.mars-hydro.com/grow-tent/buy-mars-hydro-grow-tent-24x48x70-2-x-4-cabinet-closet-grow-box-upgraded-diamond-1680d-mylar-for-sale ViparSpectra 240w Quantum Board: This thing is sleek and slick in terms of design. Closest comparable to it that I’ve seen would be spiderfarmers line of qbs. They feature the same top-o-the-line components and roughly similar spectrums. Where VS takes the advantage is in the convenience of the dimmer nob which is easily accessible and works great at 50% with no strobing👍. The heat sink is also larger which contributes to lower temps. That’s not a good thing in my case but beneficial for most folks. I can already say that this will be a great fixture through all stages of growth. Where I know this light will excel will be in the veg. Stage for sure. Dimming function gives seedlings a much better chance of success with the ability to use lower lighting levels early on and the coverage area on a 240w is great. The average grower will find this panel suitable and fitting to their needs in a 2x4 grow space although I’d suggest 2 units if going any larger than that. The total output in this space equates to about 30w/sq.ft. Which is a decent amount of coverage based on power vs. Growprint. Ideal for veg. Certainly but, in flower - I tend to run closer to 50-60w/sq.ft to achieve higher yields. Again, similar fixtures have performed admirably in the past and while this wouldn’t be considered an “entry-level” fixture, I’d recommend that any grower new to the game not skimp on lighting and start with one of these. The price points are a great value for quality LED tech. These days, so there’s little reason to even consider a lesser fixture👍. I have no detail on limiting factors for this light as yet but have heard that stock branded fixtures don’t necessarily incorporate enough red diodes. I’ve seen no issue myself but confess - I’m no lighting guru. Website link: https://www.viparspectra.com/collections/xs-series/products/xs-series-xs2000 ***8% discount when using code: “ItBudWhisper”*** Happy to have you along for the ride if you made it this far through my opening rant!!😎🤟😎🤟😎

That is my 2nd grow, so i tried some Lst. I use two tents and change positions depending on needs and size of the plants. I did half a Pack of "Monster Bud Mix" (Zamnesia) in the soil, now i feed little amount of Plagron (i dont feed every watering). She made no bigger problems (sometimes she showed signs of lightstress, iguess - i am still figuring out why), but i am a noob in this game and others would have better results. From day to day she looks more delicious. In all i like the result of this strain. 1 of 1 germinated august 28th.

Vigorous Males, Strong Clones, and Bud Formation This week was full of action in the grow! Two out of the three males are in full bloom, and I’m actively collecting pollen. They are impressive plants, with strong structures and large pollen sacs. If everything stays on track, I believe the females will be ready for pollination in about a week. Observations: All 18 clones have successfully rooted! Some have already entered the vegetative stage and are receiving supplemental light at nightfall to keep their growth steady. The plants responded very well to the light defoliation I did earlier. Now, the buds are starting to take shape, showing great progress in the flowering phase. To optimize this stage, I spaced out the pots, ensuring better airflow and increased light penetration, which should support full and healthy flowering. This week, I plan to top off the pots with more substrate, ensuring the plants have enough space and nutrients to reach their full potential. Next Steps: Keep monitoring the males and collecting pollen at the right time. Watch the females closely to determine the ideal moment for planned pollinations. Observe the clones' growth and adjust care as needed. The grow is on the right track, and expectations for the coming days are high!

4iem semaines de floraison... Tout ce passe bien 😜

- Third week Came back from my trip and she really shot up PH was down (around 5.5) so she absorbed a good amount of nutrients while I was away Changed reservoir with a dosage of Base A- Base B- Vege- Silica- using 1.0 ML per Liter Roots are nice but I'm starting to think she might have some sort of rot going on, going to introduce Peroxide in the water to help her out No sexing yet, I'm assuming she's a female (hopefully) Did some small LST in order for the bottom line to grow a little bit

So endlich freie Samen von RQS vom letzten Jahr eingepflazt...im Moment keine speziellen Dünger...nuf etwas Fishmix...Erde ist Biobizz Allmix...Pflanze scheint es zu mögen...Plan ist...so ab ca. Woche 6-7 in die freie Natur verpflanzen (guerrilla)

Looking

Getting really close now. Should be down within the week. This has been a super good grow. ILGM always comes thru with good stuff!!

So all my stuff arrived this week. Will be using fox farm ocean floor soil for this grow. I have blue dream going for this grow. My seeds are germinating and I don’t have pics of them from the paper towel and from when I first put them beneath to soil in the dixi cups. The soil I used was miracle grow bloom potting soil for my initial planting (now I know was probably not the best choice) but I actually noticed some great results to start with the grow. I’ll keep updating as I go and hope to get some great feed back and comments while I go along. Happy growing everyone.

Buds are bulking up! Watering pots separately as the soil dries out. Watered in some Microbial Mass to give the soil more available microbes.

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 44 since time change to 12/12 h. Hey guys :-) . The lady is developing very well. The buds keep getting thicker and smell better and better :-). This week it was poured twice with 1.2 l each (sewing materials, see table above). The tent was cleaned and everything checked. Stay healthy 🙏🏻 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. You can buy This Strain at : www.Zamnesia.com ☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼 Strain Gelato clone from mother (Zamnesia ) ☝️ Genetics: Wedding Cake x Gelato x Gelato 33 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Nutrients : Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4

Transplanted today added another blue CFL 250w +:125w temps gone up nicely.... Looks like it was needed! After a week I will switch to the gavita dual end once these are settled haven't got tent up they seem to be fine so far but they are touching each other

This week I change the period to 12/12. Girls looks happy

Esplosioni di cime e terpeni manca sempre meno alla fine. Concimi e nutrienti usati 25 g di guaino di pipistrello 16 ml di adv nutriens 25 g di pellet a rilascio lento rqs

Getoppt +Lst, paar Trauermücken....

I’m about to lose it. Big progress both from the broken stem and the plant as a whole. I think though the accident was very stressful and it turn it into a hermie. Once the lovely community makes sure I got a hermie I will cut the balls off(no cap). Day 36, one day after the hermie incident.I cut the sacs of and keeping a really close eye on it.Like checking every hour on different lighting again and again. Main cola looks like neither full bounced back, nor losing the battle from the harm I did to her in the middle of the fifth week,the tape is still on. I'm anxious if I face a light stress till the full recovery of the stem. I can't LST thats for sure and I have an HPS lamp. I'm aware that my yields will not be the same as the true potencial of the strain but I'm sure the taste and high will be diffrent for me due to the work I'm putting in and the "stress" I'm going through. Day 37: No sight of sacs. Looks thirsty every day and sometimes I water twice.The soil is dry one the first cm.